scholarly journals Insulin and Glucagon Impairments in Relation with Islet Cells Morphological Modifications Following Long Term Pancreatic Duct Ligation in the Rabbit – A Model of Non-insulin-dependent Diabete

2001 ◽  
Vol 2 (2) ◽  
pp. 101-112 ◽  
Author(s):  
J. Catala ◽  
M. Daumas ◽  
A. Pham Huu Chanh ◽  
B. Lasserre ◽  
E Hollande
Keyword(s):  
Pancreatic Duct ◽  
Islet Cells ◽  
Rabbit Model ◽  
Fold Increase ◽  
Pancreatic Duct Ligation ◽  
A Cells ◽  
Duct Ligation ◽  
Insulin Dependent

Plasma levels of glucose, insulin and glucagon were measured at various time intervals after pancreatic duct ligation (PDL) in rabbits. Two hyperglycemic periods were observed: one between 15–90 days (peak at 30 days of 15.1 ± 1.2mmol/l, p < 0.01), and the other at 450 days (11.2 ± 0.5 mmol/l, p < 0.02). The first hyperglycemic episode was significantly correlated with both hypoinsulinemia (41.8 ± 8pmol/l, r= –0.94, p < 0.01) and hyperglucagonemia (232 ± 21ng/l, r=0.95, p < 0.01). However, the late hyperglycemic phase (450 days), which was not accompanied by hypoinsulinemia, was observed after the hyperglucagonemia (390 days) produced by abundant immunostained A-cells giving rise to a 3-fold increase in pancreatic glucagon stores. The insulin and glucagon responses to glucose loading at 180, 270 and 450 days reflected the insensitivity of B- and A-cells to glucose. The PDL rabbit model with chronic and severe glycemic disorders due to the predominant role of glucagon mimicked key features of the NIDDM syndrome secondary to exocrine disease.

Immunocytochemistry and release of pancreatic and gut hormones after long-term pancreatic duct ligation

1980 ◽  
Vol 1 ◽  
pp. S62
Author(s):  
G Klöppel ◽  
R Klapdor ◽  
G Bommer ◽  
A Knipper ◽  
EP Schorn
Keyword(s):  
Pancreatic Duct ◽  
Gut Hormones ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

ROLE OF PANCREATIC DUCT LIGATION FOR SEGMENTAL PANCREAS AUTOTRANSPLANTATION

1979 ◽  
Vol 28 (6) ◽  
pp. 469-475 ◽  
Author(s):  
Luis H. Toledo-Pereyra ◽  
Javier Castellanos
Keyword(s):  
Pancreatic Duct ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

scholarly journals Long term follow-up of a simplified and less burdened pancreatic duct ligation model of exocrine pancreatic insufficiency in Goettingen Minipigs

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Andreas Minh Luu ◽  
Alexander Brock ◽  
Sabrina Ritz ◽  
Sandra Junghänel ◽  
Ingo Aldag ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Pancreatic Insufficiency ◽  
Pancreatic Enzyme ◽  
Exocrine Pancreatic Insufficiency ◽  
Pancreatic Ducts ◽  
Health State ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

Abstract Background Pancreatic duct ligation in a minipig model leads to exocrine pancreatic insufficiency (EPI). This allows the study of digestive processes and pancreatic enzyme replacement therapies. However, detailed descriptions of the surgical procedure, perioperative management, a determination of exocrine pancreatic insufficiency are scarce in the literature. Data of the long-term health status of minipigs upon EPI induction are still not available. Therefore, the present study describes in detail an experimental approach to the induction of exocrine pancreatic insufficiency via pancreatic duct ligation in minipigs and the long term follow up of the animal’s health state. Methods 14 Goettingen minipigs underwent pancreatic duct ligation via midline laparotomy for the induction of exocrine pancreatic insufficiency. Fecal fat content, fat absorption, chymotrypsin levels, body weight and blood vitamin and glucose levels were determined. Results Exocrine pancreatic insufficiency was successfully induced in 12 Goettingen minipigs. Two minipigs failed to develop exocrine insufficiency most likely due to undetected accessory pancreatic ducts. All animals tolerated the procedure very well and gained weight within 8 weeks after surgery without requiring pancreatic enzyme replacement therapy. The follow up for approx. 180 weeks showed a stable body weight and health state of the animals with normal blood glucose levels (Table 1). From approx. 130 weeks post pancreatic duct ligation, all animals were supplemented with pancreatic enzymes and vitamins resulting in blood concentrations almost within the reference range. Conclusions Pancreatic duct ligation in minipigs is an excellent method of inducing exocrine pancreatic insufficiency. It is important to identify and ligate accessory pancreatic ducts since persistence of accessory ducts will lead to maintenance of exocrine pancreatic function. The EPI model caused no persistent side effects in the animals and has the potential to be used in long-term EPI studies with up to 100 weeks post-OP without supplementation with enzymes and vitamins.

ROLE OF PANCREATIC DUCT LIGATION FOR SEGMENTAL PANCREAS AUTOTRANSPLANTATION

1979 ◽  
Vol 27 (6) ◽  
pp. 469-475 ◽  
Author(s):  
LUIS H. TOLEDO-PEREYRA ◽  
JAVIER CASTELLANOS
Keyword(s):  
Pancreatic Duct ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

scholarly journals Long - term follow up of a simplified and less burdened pancreatic duct ligation model of exocrine pancreatic insufficiency in Goettingen Minipigs

2020 ◽  
Author(s):  
Andreas Minh Luu ◽  
Alexander Brock ◽  
Sabrina Ritz ◽  
Sandra Junghänel ◽  
Ingo Aldag ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Pancreatic Insufficiency ◽  
Exocrine Pancreatic Insufficiency ◽  
Pancreatic Ducts ◽  
Fat Absorption ◽  
Glucose Levels ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

Abstract BackgroundPancreatic duct ligation in a minipig model leads to exocrine pancreatic insufficiency (EPI). This allows studies of digestive processes and pancreatic enzyme replacement therapies (PERT). However, detailed descriptions of the surgical procedure, perioperative management, and determination of exocrine pancreatic insufficiency are scarce in literature. To date, data of long-term health status of minipigs after induction of EPI are not available. This study describes in detail the experimental approach of EPI-induction via pancreatic duct ligation in minipigs and long-term results of the animal’s health state.Methods14 Goettingen minipigs underwent pancreatic duct ligation via midline laparotomy for the induction of exocrine pancreatic insufficiency. Fecal fat content, fat absorption, body weight, chymotrypsin levels, blood vitamin levels and glucose levels were determined. Follow-up data was gathered for a period of 180 weeks postoperatively.ResultsExocrine pancreatic insufficiency was successfully induced in 12 Goettingen minipigs. Fecal fat content increased from 9.0% to 30.9% (p < 0.001). Mean coefficient of fat absorption decreased from 90% (± 2.8) to 24.2% (± 8.3). PERT led to a significant increase of fat absorption. Two minipigs failed to develop exocrine insufficiency most likely due to undetected accessory pancreatic ducts. All animals tolerated the procedure very well and gained weight within 6 weeks past surgery. Follow up for 180 weeks showed a stable body weight and health state of the animals with normal blood glucose levels. Vitamin E and B12 levels dropped significantly between post-op week 125 and 134 requiring vitamin supplementation. ConclusionsPancreatic duct ligation in minipigs is an excellent method to induce exocrine pancreatic insufficiency. It is important to identify and ligate accessory pancreatic ducts since persistence of accessory ducts will lead to maintenance of exocrine pancreatic function. The EPI model caused no persistent side effects in the animals and can be applied in long-term EPI studies. However, it is important to substitute Vitamin B12 and E as their concentration drops in the long-term period.

scholarly journals Publisher Correction: Pancreatic duct ligation reduces premalignant pancreatic lesions in a Kras model of pancreatic adenocarcinoma in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Cáceres ◽  
Rita Quesada ◽  
Mar Iglesias ◽  
Francisco X. Real ◽  
Maria Villamonte ◽  
...  
Keyword(s):  
Pancreatic Duct ◽  
Pancreatic Adenocarcinoma ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Somatostatin levels and binding in duodenal mucosa of rabbits with ligation of the pancreatic duct

1988 ◽  
Vol 118 (2) ◽  
pp. 227-232 ◽  
Author(s):  
L. G. Guijarro ◽  
E. Arilla
Keyword(s):  
Pancreatic Duct ◽  
Binding Sites ◽  
Exocrine Pancreas ◽  
Duodenal Mucosa ◽  
Physiological Significance ◽  
Scatchard Analysis ◽  
Pancreatic Duct Ligation ◽  
Parallel Increase ◽  
Duct Ligation ◽  
Number Of Binding Sites

ABSTRACT Atrophy of the exocrine pancreas was induced in rabbits by pancreatic duct ligation. Somatostatin concentration and binding in cytosol from rabbit duodenal mucosa were studied after 6 and 14 weeks of pancreatic duct ligation. Somatostatin-like immunoreactivity was significantly increased in the duodenal mucosa in both periods. Scatchard analysis showed a parallel increase in the number of binding sites rather than a change in their affinity. The physiological significance of these findings remains to be clarified. J. Endocr. (1988) 118, 227–232

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