Abstract 763: Resistance to cabazitaxel is associated withABCB1/P-glycoprotein activation, alterations in β-tubulin content and dynamics, reduced BRCA1, and a mesenchymal phenotype in MCF-7 human breast cancer variants

2014 ◽  
Author(s):  
George E. Duran ◽  
Yan C. Wang ◽  
E Brian Francisco ◽  
Francisco J. Martinez ◽  
Branimir I. Sikic
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Mesenchymal Phenotype ◽  
P Glycoprotein ◽  
Tubulin Content ◽  
Β Tubulin ◽  
Mcf 7

scholarly journals Psoralen reverses the P-glycoprotein-mediated multidrug resistance in human breast cancer MCF-7/ADR cells

2016 ◽  
Vol 13 (6) ◽  
pp. 4745-4750 ◽  
Author(s):  
JINGRU JIANG ◽  
XIAOHONG WANG ◽  
KAI CHENG ◽  
WANZHONG ZHAO ◽  
YITONG HUA ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multidrug Resistance ◽  
Human Breast Cancer ◽  
Human Breast ◽  
P Glycoprotein ◽  
Mcf 7

A novel indirubin derivative PHII-7 potentiates adriamycin cytotoxicity via inhibiting P-glycoprotein expression in human breast cancer MCF-7/ADR cells

2011 ◽  
Vol 669 (1-3) ◽  
pp. 38-44 ◽  
Author(s):  
Ruizan Shi ◽  
Wei Li ◽  
Xiuli Zhang ◽  
Yanjun Zhang ◽  
Hongwei Peng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Human Breast ◽  
P Glycoprotein ◽  
Mcf 7

The Impact of Tetrahydropalmatine on 99Tcm-MIBI Uptake of Human Breast Cancer MCF-7 Cell

2011 ◽  
Vol 138-139 ◽  
pp. 1078-1081
Author(s):  
Jun Yun ◽  
Ling Wang ◽  
Jia Ying Yuan
Keyword(s):  
Breast Cancer ◽  
Uptake Rate ◽  
Human Breast Cancer ◽  
Multidrug Resistant ◽  
Carcinoma Cells ◽  
Human Breast ◽  
Glycoprotein P ◽  
P Glycoprotein ◽  
The Impact ◽  
Mcf 7

Objective: To observe the effects of chemosensitizer on expression of P-glycoprotein (P-gp) in multidrug-resistant (MDR) carcinoma cells and the changes of corresponding 99Tcm-MIBI (referring to methoxy isobutyl isonitrile) uptake kinetics. Methods: Firstly, the immunocytochemistry was used to test the experssion level of P-gp in carcinoma cells; then the γ counter was used to test the uptake rate of radioactive precipitation of 99Tcm-MIBI; and finally to do statistical analysis of the test data. Results: The P-gp expression in MCF-7/S cells was negative, while it was strongly positive in MCF-7/ADM cells, and the P-gp expression in MCF-7/ADM cells reduced significantly after the tetrahydropalmatine (Tet) effect. The uptake rate of MCF-7/S cells reached the maximal value (1.39%) after being cultured with 99Tcm-MIBI for 90 minutes, and then it kept in equilibrium. The 99Tcm-MIBI uptake rate of MCF-7/ADM cells had been maintained at low level, being 0.371% after 90 minutes. The 99Tcm-MIBI uptake rate of MCF-7/S cells was higher than that of MCF-7/ADM cells. After the tetrahydropalmatine and verapamil effects, the 99Tcm-MIBI uptake rate of MCF-7/S cells had no significant change, while the 99Tcm-MIBI uptake rate of MCF-7/ADM cells increased by 1.9 times. Conclusion: Tet can reverse the MDR in MCF-7 cells, and the mechanism can achieve the effect of reversing the drug resistance through adjusting down the expression of P-gp in carcinoma cells.

Effect of P-glycoprotein Expression on Sensitivity to Hormones in MCF-7 Human Breast Cancer Cells

1992 ◽  
Vol 84 (19) ◽  
pp. 1506-1512 ◽  
Author(s):  
R. Clarke ◽  
S. Currier ◽  
O. Kaplan ◽  
E. Lovelace ◽  
V. Boulay ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
P Glycoprotein ◽  
Mcf 7

scholarly journals Aged garlic extract potentiates doxorubicin cytotoxicity in human breast cancer cells

2020 ◽  
Vol 19 (8) ◽  
pp. 1669-1676
Author(s):  
Huda Mohammed Alkreathy ◽  
Noura Farraj AlShehri ◽  
Fatemah Omer Kamel ◽  
Ahmed Khalaf Alghamdi ◽  
Ahmed Esmat ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Garlic Extract ◽  
Human Breast ◽  
Aged Garlic Extract ◽  
P Glycoprotein ◽  
Aged Garlic ◽  
Mcf 7

Purpose: To investigate the potential chemo-sensitizing effect of aged garlic extract (AGE) on doxorubicin (DOX) in breast cancer cells (MCF-7), and the possible underlying mechanisms.Methods: Human breast cancer cell line (MCF-7) was treated with AGE and DOX. The cytotoxic effects of AGE and DOX were investigated via cell cycle analysis and apoptosis induction, using flow cytometry. Mechanistic studies involved the determination of cellular uptake of DOX and  p-glycoprotein (P-gp) activity.Results: Combined treatment of MCF7 cells with AGE and DOX produced no significant effect at AGE dose of 10 mg/mL. However, co-treatment with AGE at doses of 50 and 93 mg/mL enhanced the cytotoxicity of DOX on MCF-7 cells, with IC50 values of 0.962 and 0.999 μM, respectively, whencompared with 1.85 μM DOX alone. Moreover, Annexin V-FITC and PI techniques showed that AGE significantly increased percentage of cells in late apoptosis. Besides, AGE-DOX treatment significantly increased cellular uptake of DOX and inhibited P-gp activity, when compared with DOX alone (p < 0.05).Conclusion: AGE enhances the cytotoxic effect of DOX on MCF-7 cells, most likely due to cell cycle distribution, stimulation of apoptosis, increased uptake of DOX by MCF7, and inhibition of P-gp activity. Keywords: Aged garlic extract, Doxorubicin, Breast cancer, MCF-7 cell line, P-glycoprotein, Apoptosis, Cell cycle

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