A novel compound RY10-4 downregulates P-glycoprotein expression and reverses multidrug-resistant phenotype in human breast cancer MCF-7/ADR cells

2014 ◽  
Vol 68 (8) ◽  
pp. 1049-1056 ◽  
Author(s):  
Pingping Xue ◽  
Xiaofan Yang ◽  
Yang Liu ◽  
Chaomei Xiong ◽  
Jinlan Ruan
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Multidrug Resistant ◽  
Human Breast ◽  
Resistant Phenotype ◽  
P Glycoprotein ◽  
Mcf 7

The Impact of Tetrahydropalmatine on 99Tcm-MIBI Uptake of Human Breast Cancer MCF-7 Cell

2011 ◽  
Vol 138-139 ◽  
pp. 1078-1081
Author(s):  
Jun Yun ◽  
Ling Wang ◽  
Jia Ying Yuan
Keyword(s):  
Breast Cancer ◽  
Uptake Rate ◽  
Human Breast Cancer ◽  
Multidrug Resistant ◽  
Carcinoma Cells ◽  
Human Breast ◽  
Glycoprotein P ◽  
P Glycoprotein ◽  
The Impact ◽  
Mcf 7

Objective: To observe the effects of chemosensitizer on expression of P-glycoprotein (P-gp) in multidrug-resistant (MDR) carcinoma cells and the changes of corresponding 99Tcm-MIBI (referring to methoxy isobutyl isonitrile) uptake kinetics. Methods: Firstly, the immunocytochemistry was used to test the experssion level of P-gp in carcinoma cells; then the γ counter was used to test the uptake rate of radioactive precipitation of 99Tcm-MIBI; and finally to do statistical analysis of the test data. Results: The P-gp expression in MCF-7/S cells was negative, while it was strongly positive in MCF-7/ADM cells, and the P-gp expression in MCF-7/ADM cells reduced significantly after the tetrahydropalmatine (Tet) effect. The uptake rate of MCF-7/S cells reached the maximal value (1.39%) after being cultured with 99Tcm-MIBI for 90 minutes, and then it kept in equilibrium. The 99Tcm-MIBI uptake rate of MCF-7/ADM cells had been maintained at low level, being 0.371% after 90 minutes. The 99Tcm-MIBI uptake rate of MCF-7/S cells was higher than that of MCF-7/ADM cells. After the tetrahydropalmatine and verapamil effects, the 99Tcm-MIBI uptake rate of MCF-7/S cells had no significant change, while the 99Tcm-MIBI uptake rate of MCF-7/ADM cells increased by 1.9 times. Conclusion: Tet can reverse the MDR in MCF-7 cells, and the mechanism can achieve the effect of reversing the drug resistance through adjusting down the expression of P-gp in carcinoma cells.

scholarly journals Psoralen reverses the P-glycoprotein-mediated multidrug resistance in human breast cancer MCF-7/ADR cells

2016 ◽  
Vol 13 (6) ◽  
pp. 4745-4750 ◽  
Author(s):  
JINGRU JIANG ◽  
XIAOHONG WANG ◽  
KAI CHENG ◽  
WANZHONG ZHAO ◽  
YITONG HUA ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multidrug Resistance ◽  
Human Breast Cancer ◽  
Human Breast ◽  
P Glycoprotein ◽  
Mcf 7

scholarly journals Downregulation of mdr-1 expression by 8-Cl-cAMP in multidrug resistant MCF-7 human breast cancer cells.

1995 ◽  
Vol 96 (2) ◽  
pp. 1026-1034 ◽  
Author(s):  
S Scala ◽  
A Budillon ◽  
Z Zhan ◽  
Y S Cho-Chung ◽  
J Jefferson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Multidrug Resistant ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mdr 1 ◽  
Mcf 7
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