Abstract 1480: CCL4 promotes oral squamous cell carcinoma angiogenesis by targeting the angiopoietin-2 signal pathway

Background: There are various secreted proteins affecting the prognosis of oral squamous cell carcinoma (OSCC) and one of them is Angiopoietin-2(Ang-2) which is thought to have an essential role in the development and progression of the tumor. Aim of the study: This study was conducted to determine the expression of (Ang-2) in (OSCC) to assess its correlations with clinicopathological parameters of the tumor. Material and Methods: 36 formalin- fixed, paraffin- embedded tissue blocks histologically diagnosed as OSCC were examined for Ang-2 immunohistochemical expression semi quantitively. Results: The expression of Ang-2 was significantly associated with histopathological grade (P value=0.023), while there is no significant association with the clinical parameters analyzed in OSCC patients. Conclusion: A significant association between Ang-2 expression and histopathological grade of OSCC may predict its biological behavior.

Download Full-text

Expression and function of dinkkopf 4 in oral squamous cell carcinoma and in vitro

10.21203/rs.3.rs-17619/v1 ◽

2020 ◽

Author(s):

Xinjia Cai ◽

Zhigang Yao ◽

Junhui Huang

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Malignant Tumors ◽

Plasmid Vector ◽

Signal Pathway ◽

And Migration ◽

Proliferation And Migration

Abstract Background Oral squamous cell carcinoma accounts for about 90% of malignant tumors of the head and neck, and its 5-year survival is less than 50%. The number of new cases of oral cancer worldwide is expected to increase by 62% in 2035. Wnt/β-catenin signal pathway plays a role in the tumorigenesis and progression of cancer. dinkkopf 4 is involved in a variety of cancers as a molecule in Wnt/β-catenin signal. To our knowledge, the study of DKK4 in OSCC has not been reported. Methods The well differentiated oral squamous cell carcinoma and normal oral mucosa samples were collected from Department of Pathology of Xiangya Stomatological Hospital of Central South University. Human oral epithelial cell HOEC and human oral squamous cell carcinoma cell TSCC1 were cultured for experiments. The plasmid vector was constructed to inhibit the expression of DKK4 to form TSCC1-shDKK4 cells and TSCC1-NC cells were transfected with blank plasmid. Cell proliferation was detected by CCK-8 test. Apoptosis was detected by Annexin V-FITC/PI assay. Cell migration was detected by Transwell assay. IBM SPSS Statistics 24.0 and GraphPad Prism 8 were performed for statistical analysis and graphing. Results We found that the expression of DKK4 and β-catenin increased in OSCC and in vitro (P<0.05). dinkkopf 4 may promote the expression of β-catenin in OSCC. The proliferation and migration of TSCC1-shDKK4 cells decreased and the apoptosis of TSCC1-shDKK4 cells increased (P<0.01). Conclusions dinkkopf 4 may promote the proliferation and migration of OSCC cells and inhibit the apoptosis of OSCC cells in vitro by regulating Wnt/β-catenin signal pathway.

Download Full-text