Abstract B12: HPV-positive tumor cell lines exhibit major alterations in Toll-like receptor pathways and depend on HMGB1 expression

2018 ◽  
Author(s):  
Mirian Galliote Morale ◽  
Walason da Silva Abjaude ◽  
Aline Montenegro da Silva ◽  
Luisa Lina Villa ◽  
Enrique Boccardo
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Toll Like Receptor ◽  
Positive Tumor Cell ◽  
Hmgb1 Expression

scholarly journals Isolation of rabbit single domain antibodies to B7-H3 via protein immunization and phage display

2020 ◽  
Vol 3 (1) ◽  
pp. 10-17
Author(s):  
Ruonan Feng ◽  
Ruixue Wang ◽  
Jessica Hong ◽  
Christopher M Dower ◽  
Brad St Croix ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Phage Display ◽  
Cell Lines ◽  
Single Domain ◽  
Tumor Cell Lines ◽  
Positive Tumor Cell ◽  
Drug Candidates ◽  
Potential Applications ◽  
Single Domain Antibodies ◽  
Protein Immunization

Abstract Single domain antibodies have certain advantages including their small size, high stability and excellent tissue penetration, making them attractive drug candidates. Rabbit antibodies can recognize diverse epitopes, including those that are poorly immunogenic in mice and humans. In the present study, we established a method to isolate rabbit VH single domain antibodies for potential cancer therapy. We immunized rabbits with recombinant human B7-H3 (CD276) protein, made a phage-displayed rabbit VH single domain library with a diversity of 7 × 109, and isolated two binders (A1 and B1; also called RFA1 and RFB1) from phage panning. Both rabbit VH single domains exhibited antigen-dependent binding to B7-H3-positive tumor cell lines but not B7-H3 knockout tumor cell lines. Our study shows that protein immunization followed by phage display screening can be used to isolate rabbit single domain antibodies. The two single domain antibodies reported here may have potential applications in cancer immunotherapy.

In vitroevaluation of99mTc-EDDA/tricine-HYNIC-Q-Litorin in gastrin-releasing peptide receptor positive tumor cell lines

2013 ◽  
Vol 21 (4) ◽  
pp. 383-388 ◽  
Author(s):  
Fatma Yurt Lambrecht ◽  
Kübra Durkan ◽  
Aykut Özgür ◽  
Cumhur Gündüz ◽  
Çığır Biray Avcı ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Positive Tumor Cell ◽  
Peptide Receptor ◽  
Gastrin Releasing Peptide

Small interfering RNA (siRNA) inhibits the expression of the Her2/neu gene, upregulates HLA class I and induces apoptosis of Her2/neu positive tumor cell lines

2003 ◽  
Vol 108 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Aniruddha Choudhury ◽  
Jehad Charo ◽  
Sunil K. Parapuram ◽  
Richard C. Hunt ◽  
D. Margaret Hunt ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Small Interfering Rna ◽  
Hla Class I ◽  
Class I ◽  
Tumor Cell Lines ◽  
Positive Tumor Cell ◽  
Interfering Rna

Interaction of Human Tumor Cells with Human Platelets and the Coagulation System

1983 ◽  
Vol 50 (03) ◽  
pp. 726-730 ◽  
Author(s):  
Hamid Al-Mondhiry ◽  
Virginia McGarvey ◽  
Kim Leitzel
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Human Tumor ◽  
Human Platelets ◽  
Factor Vii ◽  
Coagulation System ◽  
Breast Adenocarcinoma ◽  
Activate Factor ◽  
Tumor Cell Lines

SummaryThis paper reports studies on the interaction between human platelets, the plasma coagulation system, and two human tumor cell lines grown in tissue culture: Melanoma and breast adenocarcinoma. The interaction was monitored through the use of 125I- labelled fibrinogen, which measures both thrombin activity generated by cell-plasma interaction and fibrin/fibrinogen binding to platelets and tumor cells. Each tumor cell line activates both the platelets and the coagulation system simultaneously resulting in the generation of thrombin or thrombin-like activity. The melanoma cells activate the coagulation system through “the extrinsic pathway” with a tissue factor-like effect on factor VII, but the breast tumor seems to activate factor X directly. Both tumor cell lines activate platelets to “make available” a platelet- derived procoagulant material necessary for the conversion of prothrombin to thrombin. The tumor-derived procoagulant activity and the platelet aggregating potential of cells do not seem to be inter-related, and they are not specific to malignant cells.

Differential Increases in Glutathione S-Transferase Activities in a Range of Multidrug-Resistant Human Tumor Cell Lines

1989 ◽  
Vol 1 (6) ◽  
pp. 359-365 ◽  
Author(s):  
Richard D. H. Whelan ◽  
Louise K. Hosking ◽  
Alan J. Townsend ◽  
Kenneth H. Cowan ◽  
Bridget T. Hill
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Human Tumor ◽  
Multidrug Resistant ◽  
Tumor Cell Lines ◽  
Glutathione S Transferase ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines

Cytotoxic Activities of Red Algae Collected from Jeju Island Against Four Tumor Cell Lines

2006 ◽  
Vol 11 (3) ◽  
pp. 177-183 ◽  
Author(s):  
Kil-Nam Kim ◽  
Ki-Wan Lee ◽  
Choon-Bok Song ◽  
Chang-Bum Ahn ◽  
You-Jin Jeon
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Red Algae ◽  
Jeju Island ◽  
Cytotoxic Activities ◽  
Tumor Cell Lines
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