Analysis of the 5’ End Structure of HCV Subgenomic RNA Replicated in a Huh7 Cell Line

Intervirology ◽  
2005 ◽  
Vol 48 (2-3) ◽  
pp. 104-111 ◽  
Author(s):  
Hitoshi Takahashi ◽  
Masashi Yamaji ◽  
Masahiro Hosaka ◽  
Hiroe Kishine ◽  
Makoto Hijikata ◽  
...  
Keyword(s):  
Cell Line ◽  
Huh7 Cell ◽  
Subgenomic Rna ◽  
Huh7 Cell Line

Optimization of in vitro HBV replication and HBsAg production in HuH7 cell line

2013 ◽  
Vol 189 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Daniela Cavallone ◽  
Francesco Moriconi ◽  
Piero Colombatto ◽  
Filippo Oliveri ◽  
Ferruccio Bonino ◽  
...  
Keyword(s):  
Cell Line ◽  
Huh7 Cell ◽  
Hbv Replication ◽  
Huh7 Cell Line

scholarly journals Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway

2020 ◽  
Vol 9 ◽  
pp. 1896
Author(s):  
Maedeh Olya ◽  
Hamid Zaferani Arani ◽  
Amirhossein Shekarriz ◽  
Amirhossein Zabolian ◽  
Hadi Zare Marzouni ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Flow Cytometry ◽  
Liver Cancer ◽  
Cell Line ◽  
Real Time ◽  
Real Time Pcr ◽  
Huh7 Cell ◽  
P53 Expression ◽  
Huh7 Cells ◽  
Huh7 Cell Line

Background: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present study aimed to evaluate the apoptotic effect of hypericin on the Huh7 as the liver cancer cell line and its relation with the gate keeper gene P53. Materials and Methods: In this study, the Huh7 cell line and fibroblast cells (as control group) were treated with different concentrations of hypericin for 24 and 48 hours. Detection of cell death was performed by MTT assay and flow cytometry. The expression of bax, bcl2 and p53 mRNAs was evaluated by Real-time PCR. Also, Immunocytochemistry (ICC) analysis was used for further evaluation of P53expression. Results: The results showed that hypericin has a dose-dependent cytotoxic effect on the Huh7 cell line, with no or marginal effect on fibroblastic cells. According to flow cytometry results, about 53%cells underwent apoptosis after exposure to LD50 of hypericin for 24 hours. Real-time PCR data demonstrated that the pro-apoptotic genes Bax and P53 expression level increased. Expectedly ICC results confirmed the up-regulation of P53 proteins in treated samples. Conclusion: Our results indicate the cytotoxicity of hypericin on Huh7 cells by affecting the expression of the gate keeper gene P53; furthermore it is suggested that this herb can be utilized simultaneously with modalities targeting P53 up-regulation or related molecular pathways. [GMJ.2020;9:e1896]

3 IMPROVED IN VITRO HBV REPLICATION IN HuH7 CELL LINE TRANSFECTED WITH FULL LENGTH CIRCULAR CLOSED HBV DNA BY MODIFICATIONS OF THE GUNTHER'S METHOD

2010 ◽  
Vol 42 ◽  
pp. S2 ◽  
Author(s):  
D. Cavallone ◽  
P. Colombatto ◽  
F. Moriconi ◽  
A. Stabile ◽  
A.M. Maina ◽  
...  
Keyword(s):  
Cell Line ◽  
Huh7 Cell ◽  
Hbv Dna ◽  
Full Length ◽  
Hbv Replication ◽  
Huh7 Cell Line

scholarly journals Thiazolidinedione or Rhodanine: A Study on Synthesis and Anticancer Activity Comparison of Novel Thiazole Derivatives

2017 ◽  
Vol 20 (1) ◽  
pp. 415 ◽  
Author(s):  
Cigdem Ozen ◽  
Meltem Ceylan Unlusoy ◽  
Nazanin Aliary ◽  
Mehmet Ozturk ◽  
Oya Bozdag Dundar
Keyword(s):  
Hepatocellular Carcinoma ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Condensation Reaction ◽  
Huh7 Cell ◽  
Anticancer Activities ◽  
Thiazole Derivatives ◽  
Ic50 Values ◽  
Huh7 Cell Line

Purpose: A new series of thiazolyl-2,4-thiazolidinedione / rhodanine compounds T1-T23 was synthesized and tested for their anticancer activities. Hepatocellular carcinoma cell lines were chosen due to their strong drug resistance to test the new compounds. Methods: All compounds were synthesized via Knoevenagel Condensation reaction and thiazolidinedione ester compounds (T3,T9,T15,T20) were hydrolyzed for obtaining the acidic compounds (T6,T12,T17,T23). All compounds were firstly screened for their anticancer activity against two hepatocellular carcinoma (HCC) cell lines, Huh7 and Plc/Prf/5 (Plc) cell lines by sulforhodamine B assay. Further IC50 values were calculated for three candidates (T4, T15, T21) in five different HCC (Huh7, Plc, Snu449, HepG2, Hep3B) and one breast cancer (Mcf7) cell line. Results: Compounds T4, T15, T21 had very strong anticancer effects even though their 10 µM concentration in Huh7 cell line. According to IC50 values, T21 was the most effective compound with IC50 values in a range from 2 to 16 µM in 6 cancer cell lines. In terms of cytotoxicity T21 mostly affected Huh7 and interestingly it was less effective against Plc. Conclusions: Considering these results it can be suggested that compounds T4, T15 and T21 may lead to the development of more potent anticancer drugs in the future. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals Identification of Characteristic Genomic Markers in Human Hepatoma Huh7 and Huh7.5.1-8 Cell Lines

2020 ◽  
Author(s):  
Masaki Kawamoto ◽  
Toshiyuki Yamaji ◽  
Kyoko Saito ◽  
Kazuhiro Satomura ◽  
Toshinori Endo ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Huh7 Cell ◽  
Host Cells ◽  
Human Hepatoma ◽  
Loss Of Function ◽  
Genomic Deletions ◽  
Huh7 Cells ◽  
Huh7 Cell Line

AbstractThe human hepatoma-derived Huh7 cell line and its derivatives (Huh7.5 and Huh7.5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these cell lines represent host cells suitable for propagating the hepatitis C virus (HCV) in vitro. The Huh7.5.1-8 cell line, a subline of Huh7.5.1, can propagate HCV more efficiently than its parental cells. To provide genomic information for cells’ quality control, we performed whole-genome sequencing of Huh7 and Huh7.5.1-8 and identified their characteristic genomic deletions, some of which are applicable to an in-house test for cell authentication. Among the genes related to HCV infection and replication, 53 genes were found to carry missense or loss-of-function mutations likely specific to the Huh7 and/or Huh7.5.1-8. Eight genes, including DDX58 (RIG-I), BAX, EP300, and SPP1 (osteopontin), contained mutations observed only in Huh7.5.1-8 or mutations with higher frequency in Huh7.5.1-8. These mutations might be relevant to phenotypic differences between the two cell lines and may also serve as genetic markers to distinguish Huh7.5.1-8 cells from the ancestral Huh7 cells.

Amino-functionalized nanoparticles as a platform for mTOR activity modulation in hepatocellular carcinoma Huh7 cell line

2017 ◽  
Vol 66 (1) ◽  
pp. S645-S646
Author(s):  
M. Lunova ◽  
V. Zablotskii ◽  
A. Prokhorov ◽  
M. Jirsa ◽  
M. Hof ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Huh7 Cell ◽  
Functionalized Nanoparticles ◽  
Huh7 Cell Line ◽  
Mtor Activity

scholarly journals Cell Clones Selected from the Huh7 Human Hepatoma Cell Line Support Efficient Replication of a Subgenomic GB Virus B Replicon

2002 ◽  
Vol 76 (15) ◽  
pp. 7736-7746 ◽  
Author(s):  
Amedeo De Tomassi ◽  
Maura Pizzuti ◽  
Rita Graziani ◽  
Andrea Sbardellati ◽  
Sergio Altamura ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Subgenomic Rna ◽  
Human Hepatoma Cell Line ◽  
Huh7 Cells

ABSTRACT Tamarins (Saguinus species) infected by GB virus B (GBV-B) have recently been proposed as an acceptable surrogate model for hepatitis C virus (HCV) infection. The availability of infectious genomic molecular clones of both viruses will permit chimeric constructs to be tested for viability in animals. Studies in cells with parental and chimeric constructs would also be very useful for both basic research and drug discovery. For this purpose, a convenient host cell type supporting replication of in vitro-transcribed GBV-B RNA should be identified. We constructed a GBV-B subgenomic selectable replicon based on the sequence of a genomic molecular clone proved to sustain infection in tamarins. The corresponding in vitro-transcribed RNA was used to transfect the Huh7 human hepatoma cell line, and intracellular replication of transfected RNA was shown to occur, even though in a small percentage of transfected cells, giving rise to antibiotic-resistant clones. Sequence analysis of GBV-B RNA from some of those clones showed no adaptive mutations with respect to the input sequence, whereas the host cells sustained higher GBV-B RNA replication than the original Huh7 cells. The enhancement of replication depending on host cell was shown to be a feature common to the majority of clones selected. The replication of GBV-B subgenomic RNA was susceptible to inhibition by known inhibitors of HCV to a level similar to that of HCV subgenomic RNA.

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