A Comprehensive Review on the Role of Various Materials in the Osteogenic Differentiation of Mesenchymal Stem Cells with a Special Focus on the Association of Heat Shock Proteins and Nanoparticles

2014 ◽  
Vol 199 (2-3) ◽  
pp. 81-102 ◽  
Author(s):  
Supriya Patil ◽  
Subhankar Paul
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Osteogenic Differentiation ◽  
Special Focus ◽  
Comprehensive Review ◽  
Shock Proteins

scholarly journals Role of Extracellular Vesicles in Compromising Cellular Resilience to Environmental Stressors

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Mona G. Alharbi ◽  
Seok Hee Lee ◽  
Aaser M. Abdelazim ◽  
Islam M. Saadeldin ◽  
Mosleh M. Abomughaid
Keyword(s):  
Stem Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Extracellular Vesicles ◽  
Special Focus ◽  
Cellular Protection ◽  
Small Regulatory Rnas ◽  
Shock Proteins ◽  
Physical And Chemical

Extracellular vesicles (EVs), like exosomes, are nanosized membrane-enveloped vesicles containing different bioactive cargo, such as proteins, lipids, mRNA, miRNA, and other small regulatory RNAs. Cell-derived EVs, including EVs originating from stem cells, may capture components from damaged cells or cells impacted by therapeutic treatments. Interestingly, EVs derived from stem cells can be preconditioned to produce and secrete EVs with different therapeutic properties, particularly with respect to heat-shock proteins and other molecular cargo contents. This behavior is consistent with stem cells that also respond differently to various microenvironments. Heat-shock proteins play roles in cellular protection and mediate cellular resistance to radiotherapy, chemotherapy, and heat shock. This review highlights the possible roles EVs play in mediating cellular plasticity and survival when exposed to different physical and chemical stressors, with a special focus on the respiratory distress due to the air pollution.

Potential role of heat shock proteins in neural differentiation of murine embryonal carcinoma stem cells (P19)

2011 ◽  
Vol 35 (7) ◽  
pp. 713-720 ◽  
Author(s):  
Elahe Afzal ◽  
Marzieh Ebrahimi ◽  
S. Mahmoud Arab Najafi ◽  
Arezoo Daryadel ◽  
Hossein Baharvand
Keyword(s):  
Stem Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Neural Differentiation ◽  
Potential Role ◽  
Embryonal Carcinoma ◽  
Shock Proteins

Expression of heat shock proteins (HSPs) in caprine bone marrow- derived mesenchymal stem cells

2020 ◽  
Vol 26 (1) ◽  
pp. 128
Author(s):  
D. Jena ◽  
S.D. Kharche ◽  
K. Gururaj ◽  
S.P. Singh ◽  
Sonam Rani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Shock Proteins

scholarly journals MicroRNA treatment modulates osteogenic differentiation potential of mesenchymal stem cells derived from human chorion and placenta

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kulisara Marupanthorn ◽  
Chairat Tantrawatpan ◽  
Pakpoom Kheolamai ◽  
Duangrat Tantikanlayaporn ◽  
Sirikul Manochantr
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factor ◽  
Osteogenic Differentiation ◽  
Differentiation Potential ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

AbstractMesenchymal stem cells (MSCs) are important in regenerative medicine because of their potential for multi-differentiation. Bone marrow, chorion and placenta have all been suggested as potential sources for clinical application. However, the osteogenic differentiation potential of MSCs derived from chorion or placenta is not very efficient. Bone morphogenetic protein-2 (BMP-2) plays an important role in bone development. Its effect on osteogenic augmentation has been addressed in several studies. Recent studies have also shown a relationship between miRNAs and osteogenesis. We hypothesized that miRNAs targeted to Runt-related transcription factor 2 (Runx-2), a major transcription factor of osteogenesis, are responsible for regulating the differentiation of MSCs into osteoblasts. This study examines the effect of BMP-2 on the osteogenic differentiation of MSCs isolated from chorion and placenta in comparison to bone marrow-derived MSCs and investigates the role of miRNAs in the osteogenic differentiation of MSCs from these sources. MSCs were isolated from human bone marrow, chorion and placenta. The osteogenic differentiation potential after BMP-2 treatment was examined using ALP staining, ALP activity assay, and osteogenic gene expression. Candidate miRNAs were selected and their expression levels during osteoblastic differentiation were examined using real-time RT-PCR. The role of these miRNAs in osteogenesis was investigated by transfection with specific miRNA inhibitors. The level of osteogenic differentiation was monitored after anti-miRNA treatment. MSCs isolated from chorion and placenta exhibited self-renewal capacity and multi-lineage differentiation potential similar to MSCs isolated from bone marrow. BMP-2 treated MSCs showed higher ALP levels and osteogenic gene expression compared to untreated MSCs. All investigated miRNAs (miR-31, miR-106a and miR148) were consistently downregulated during the process of osteogenic differentiation. After treatment with miRNA inhibitors, ALP activity and osteogenic gene expression increased over the time of osteogenic differentiation. BMP-2 has a positive effect on osteogenic differentiation of chorion- and placenta-derived MSCs. The inhibition of specific miRNAs enhanced the osteogenic differentiation capacity of various MSCs in culture and this strategy might be used to promote bone regeneration. However, further in vivo experiments are required to assess the validity of this approach.

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