Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs

2022 ◽  
Author(s):  
Woo Keyoung Kim ◽  
Wan Hee Kim ◽  
Oh-Kyeong Kweon ◽  
Byung-Jae Kang
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Acute Spinal Cord Injury ◽  
Cord Injury ◽  
Shock Proteins

The potential role of heat shock proteins in acute spinal cord injury

2014 ◽  
Vol 23 (7) ◽  
pp. 1480-1490 ◽  
Author(s):  
Yijun Zhou ◽  
Leilei Xu ◽  
Xinghua Song ◽  
Liwen Ding ◽  
Jiangtao Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Potential Role ◽  
Acute Spinal Cord Injury ◽  
Cord Injury ◽  
Shock Proteins

scholarly journals Erratum to: The potential role of heat shock proteins in acute spinal cord injury

2014 ◽  
Vol 23 (7) ◽  
pp. 1582-1582
Author(s):  
Yijun Zhou ◽  
Leilei Xu ◽  
Xinghua Song ◽  
Liwen Ding ◽  
Jiangtao Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Potential Role ◽  
Acute Spinal Cord Injury ◽  
Cord Injury ◽  
Shock Proteins

scholarly journals The role of heat shock proteins in spinal cord injury

2008 ◽  
Vol 25 (5) ◽  
pp. E4 ◽  
Author(s):  
Sangala Jaypal Reddy ◽  
Frank La Marca ◽  
Paul Park
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Motor Neurons ◽  
Ependymal Cells ◽  
Neuroprotective Effects ◽  
Experimental Drugs ◽  
Cord Injury ◽  
Shock Proteins

Heat shock proteins (HSPs) are normal intracellular proteins that are produced in greater amounts when cells are subjected to stress or injury. These proteins have been shown to play a key role in the modulation of the secondary injury that occurs after the initial spinal cord injury (SCI). Heat shock proteins normally act as molecular chaperones and are called protein guardians because they act to repair partially damaged proteins. Normally intracellular, HSPs can also be liberated into the systemic circulation to act as important inflammatory mediators. In the setting of SCI, HSP induction has been shown to be beneficial. These proteins are liberated primarily by acutely stressed microglial, endothelial, and ependymal cells. Heat shock proteins have also been shown to assist in the protection of motor neurons and to prevent chronic inflammation after SCI. In animal models, several experimental drugs have shown neuroprotective effects in the spinal cord and appear to function by modulating HSPs.

scholarly journals Effect of mesenchymal stem cell-incorporated hydroxyapatite-collagen scaffold on tissue repair in acute spinal cord injury, and the mechanism involved

2020 ◽  
Vol 19 (5) ◽  
pp. 1099-1103
Author(s):  
Zhipeng Yao ◽  
Wenge Liu ◽  
Chenyang Song
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Tissue Repair ◽  
Acute Spinal Cord Injury ◽  
Bone Marrow Mscs ◽  
Significant Difference ◽  
Cord Injury

Purpose: To study the effect of hydroxyapatite-collagen (HC) scaffold with mesenchymal stem cells (MSCs) on tissue repair in acute spinal cord  injury (SCI).Method: Adult female Sprague-Dawley rats weighing 200 - 230 g were randomly divided into two groups implanted either with bone marrow-MSCs (experimental group) or HC scaffold alone (control group). Spinal cord injury was induced using laminectomy, resulting in a 2.0-mm gap at T10 of the spinal cord. The gap was filled in both groups with 2-mm HC scaffold at day 10 of culture. Cellular development, viability, and proliferation inside the scaffold were determined. Angiogenesis was determined by measuring fibronectin (FN) immunofluorescence, von Willebrand factor (vWF), hypoxiainducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF).Results: HC scaffold strengthened MSCs. Bone marrow MSCs exhibited no statistically significant difference when compared with cells in culture at day 10 (47.03 ± 3.135 %, p > 0.05). Moreover, on days 5 and 10, FN deposition was higher in MSCs with scaffold than in scaffold-free MSCs. The expressions of FN, vWF, HIF-1α and VEGF were positively correlated, indicating that incorporation of HC scaffold into MSCs significantly improved tissue repair by improving angiogenesis via a differentiation process (p < 0.001).Conclusion: These findings suggest that HC scaffold with MSCs is a potential therapeutic procedure for spinal cord injury. Keywords: Mesenchymal stem cells, Hydroxyapatite-collagen, Spinal cord injury, HC scaffold

scholarly journals Intravenous Infusion of Mesenchymal Stem Cells Alters Motor Cortex Gene Expression in a Rat Model of Acute Spinal Cord Injury

2019 ◽  
Vol 36 (3) ◽  
pp. 411-420 ◽  
Author(s):  
Tsutomu Oshigiri ◽  
Toru Sasaki ◽  
Masanori Sasaki ◽  
Yuko Kataoka-Sasaki ◽  
Masahito Nakazaki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Motor Cortex ◽  
Rat Model ◽  
Intravenous Infusion ◽  
Acute Spinal Cord Injury ◽  
Cord Injury

scholarly journals Bone Marrow Mesenchymal Stem Cells Transplantation on Acute Spinal Cord Injury

2020 ◽  
Vol 29 (2) ◽  
pp. 91-98
Author(s):  
Hua Song ◽  
Shiqi Suo ◽  
Chao Ning ◽  
Yang Zhang ◽  
Weidong Mu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Acute Spinal Cord Injury ◽  
Cord Injury ◽  
Marrow Mesenchymal Stem Cells ◽  
Stem Cells Transplantation

Trehalose protects against spinal cord injury through regulating heat shock proteins 27 and 70 and caspase-3 genes expression

2019 ◽  
Vol 31 (1) ◽  
Author(s):  
Roya Nasouti ◽  
Mohammad Khaksari ◽  
Moghaddameh Mirzaee ◽  
Mahdieh Nazari-Robati
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Caspase 3 ◽  
Male Rats ◽  
Vehicle Group ◽  
Genes Expression ◽  
Cord Injury ◽  
Shock Proteins

AbstractBackgroundHeat shock proteins (HSPs) are a class of highly conserved proteins responsible for various functions critical to cell survival. Pharmacological induction of HSPs has been implicated in the regulation of neuronal loss and functional deficits in peripheral and central nervous system injuries. Accordingly, the present study was conducted to investigate the effect of trehalose on spinal expression of HSP27, HSP70 and caspase-3 genes following traumatic spinal cord injury (SCI) in rats.MethodsMale rats weighing 250–300 g underwent laminectomy and were divided into four groups including sham, SCI (received SCI), vehicle (received SCI and phosphate buffer saline intrathecally) and trehalose (received 10 mM trehalose intrathecally following SCI). On days 1, 3 and 7 after injury, HSP27, HSP70 and caspase-3 genes transcripts were quantified in spinal cord tissues via a real-time PCR technique. In addition, locomotor function was assessed using the Basso, Beattie and Bresnahan (BBB) rating scale.ResultsSCI induced the expression of HSP27, HSP70 and caspase-3 genes and BBB score at all time points. Trehalose treatment upregulated HSP27, HSP70 genes expression at 1 day after SCI. Interestingly, a significant reduction in the expression of HSP27 and HSP70 genes was observed on days 3 and 7 following trauma compared with the vehicle group (p < 0.01). Caspase-3 gene showed a decrease in expression in the trehalose-treated group at all times. In addition, neurological function revealed an improvement after treatment with trehalose.ConclusionThis study suggests that the neuroprotective effect of trehalose is mediated via regulation of HSP27 and HSP70, which are involved in cytoprotection and functional recovery following SCI.

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