scholarly journals Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis

2017 ◽  
Vol 27 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Huayou Chen ◽  
Jawad Ullah ◽  
Jinru Jia
Keyword(s):  
Bacillus Subtilis ◽  
Industrial Development ◽  
Vaccine Development ◽  
Low Cost ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coat Proteins ◽  
Spore Surface ◽  
Spore Surface Display ◽  
Alkaline Tolerance

Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, susceptibility to heat, pH, and chemicals. Hence, spores are the best choice to avoid these problems, and they have various applications over nonspore formers due to amenability for laboratory purposes. Various strains of <i>Clostridium</i> and <i>Bacillus</i> are spore formers, but the most suitable choice for display is <i>Bacillus subtilis</i> because, according to the WHO, it is safe to humans and considered as “GRAS” (generally recognized as safe). This review focuses on the application of spore surface display towards industries, vaccine development, the environment, and peptide library construction, with cell surface display for enhanced protein expression and high enzymatic activity. Different vectors, coat proteins, and statistical analyses can be used for linker selection to obtain greater expression and high activity of the displayed protein.

Bacillus subtilis Spore Surface Display of Haloalkane Dehalogenase DhaA

2019 ◽  
Vol 76 (10) ◽  
pp. 1161-1167 ◽  
Author(s):  
Fuli Wang ◽  
Tianyu Song ◽  
Hui Jiang ◽  
Chengxin Pei ◽  
Qibin Huang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Haloalkane Dehalogenase ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display

scholarly journals Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

2019 ◽  
Vol 29 (2) ◽  
pp. 179-190 ◽  
Author(s):  
Zhang Guoyan ◽  
An Yingfeng ◽  
Hossain M Zabed ◽  
Guo Qi ◽  
Miaomiao Yang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display ◽  
Display Technology

scholarly journals A system of vectors for Bacillus subtilis spore surface display

2014 ◽  
Vol 13 (1) ◽  
pp. 30 ◽  
Author(s):  
Adam Iwanicki ◽  
Iwona Piątek ◽  
Małgorzata Stasiłojć ◽  
Anna Grela ◽  
Tomasz Łęga ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display

scholarly journals Bacillus subtilis spore surface display of photodecarboxylase for the transformation of lipids to hydrocarbons

2021 ◽  
Vol 5 (6) ◽  
pp. 1727-1733
Author(s):  
Marianna Karava ◽  
Peter Gockel ◽  
Johannes Kabisch
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Efficient Strategy ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display ◽  
Cost Efficient

We suggest spore display as a simple and cost efficient strategy for the production of immobilized photodecarboxylase utilized for the conversion of oils to biofuels.

scholarly journals Bacillus subtilis Spore Coat

1999 ◽  
Vol 63 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Adam Driks
Keyword(s):  
Bacillus Subtilis ◽  
Posttranslational Processing ◽  
Spore Coat ◽  
Coat Proteins ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Cell Type ◽  
Dormant Cell ◽  
Bacillus Subtilis Spore ◽  
Specialized Program

SUMMARY In response to starvation, bacilli and clostridia undergo a specialized program of development that results in the production of a highly resistant dormant cell type known as the spore. A proteinacious shell, called the coat, encases the spore and plays a major role in spore survival. The coat is composed of over 25 polypeptide species, organized into several morphologically distinct layers. The mechanisms that guide coat assembly have been largely unknown until recently. We now know that proper formation of the coat relies on the genetic program that guides the synthesis of spore components during development as well as on morphogenetic proteins dedicated to coat assembly. Over 20 structural and morphogenetic genes have been cloned. In this review, we consider the contributions of the known coat and morphogenetic proteins to coat function and assembly. We present a model that describes how morphogenetic proteins direct coat assembly to the specific subcellular site of the nascent spore surface and how they establish the coat layers. We also discuss the importance of posttranslational processing of coat proteins in coat morphogenesis. Finally, we review some of the major outstanding questions in the field.

Spore Surface Display

2016 ◽  
pp. 349-366
Author(s):  
Rachele Isticato ◽  
Ezio Ricca
Keyword(s):  
Surface Display ◽  
Spore Surface ◽  
Spore Surface Display

scholarly journals Surface Display of Antigen Protein VP8* of Porcine Rotavirus on Bacillus Subtilis Spores Using CotB as a Fusion Partner

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3793 ◽  
Author(s):  
Wanqiang Li ◽  
Jie Feng ◽  
Jiajun Li ◽  
Jianzhen Li ◽  
Zhenhua Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Strain ◽  
Shuttle Vector ◽  
Surface Display ◽  
Mucosal Vaccine ◽  
Viral Gastroenteritis ◽  
Fusion Partner ◽  
Spore Surface ◽  
Effective Measure ◽  
Porcine Rotavirus

Porcine rotavirus is a major cause of acute viral gastroenteritis in suckling piglets, and vaccination is considered to be an effective measure to control these infections. The development of a live mucosal vaccine using Bacillus subtilis spores as an antigen delivery vehicle is a convenient and attractive vaccination strategy against porcine rotavirus. In this study, a shuttle vector was constructed for the spore surface display of the spike protein VP8* from porcine rotavirus (the genotype was G5P[7]). A successful display of the CotB-VP8* fusion protein on the spore surface was confirmed by Western blot and immunofluorescence microscopy analysis. The capacity for immune response generated after immunization with the recombinant strain was evaluated in a mouse model. The intestinal fecal IgA and serum IgG were detected by enzyme-linked-immunosorbent serologic assay (ELISA). Importantly, recombinant strain spores could elicit strong specific mucosal and humoral immune responses. These encouraging results suggest that recombinant B. subtilis BV could provide a strategy for a potential novel application approach to the development of a new and safe mucosal subunit vaccine against porcine rotavirus.

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