Significance of High-Risk Human Papillomavirus Testing for Atypical Glandular Cells on Cervical Cytology

2018 ◽  
Vol 62 (5-6) ◽  
pp. 405-410 ◽  
Author(s):  
Hiroaki Komatsu ◽  
Tetsuro Oishi ◽  
Daiken Osaku ◽  
Mayumi Sawada ◽  
Akiko Kudoh ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Intraepithelial Neoplasia ◽  
Adenocarcinoma In Situ ◽  
Diagnostic Significance ◽  
Predictive Values ◽  
Squamous Cells ◽  
Glandular Cells ◽  
Atypical Glandular Cells

Objectives: The aim of this study was to evaluate the diagnostic significance of high-risk human papillomavirus (hrHPV) testing for managing women with atypical glandular cells (AGC) and to explore the distribution of hrHPV genotypes. Methods: We analyzed cytologic and histopathologic diagnoses in patients referred to our institution due to AGC or atypical squamous cells of undetermined significance (ASC-US). All patients underwent hrHPV testing and genotyping, and positive (PPV) and negative predictive values (NPV) for cervical intraepithelial neoplasia (CIN) 2 or worse [CIN2+/adenocarcinoma in situ (AIS)+] were calculated. Results: Among 41 cases previously diagnosed with AGC, 22 (53%) were classified as CIN2+ (2 squamous cell carcinomas), whereas only 2 were AIS or adenocarcinoma. Twenty-seven (65.8%) cases in the AGC group were hrHPV positive. The most frequent genotypes in both the ASC-US and AGC groups were HPV16 and HPV52. The PPV of hrHPV testing for CIN2+/AIS+ was significantly higher in the AGC than in the ASC-US group (74.1 vs. 35.0%; p = 0.0005). The NPV for CIN2+/AIS+ was significantly lower in the AGC than in the ASC-US group (74.4 vs. 100%; p = 0.0441). Conclusion: In patients with AGC, both glandular and squamous lesions must be monitored. hrHPV testing is useful for detecting CIN2+/AIS+ in AGC.

Human papillomavirus DNA detection and histological findings in women referred for atypical glandular cells or adenocarcinoma in situ in their Pap smears

2004 ◽  
Vol 95 (3) ◽  
pp. 618-623 ◽  
Author(s):  
Sophie Françoise Mauricette Derchain ◽  
Silvia Helena Rabelo-Santos ◽  
Luis Otávio Sarian ◽  
Luiz Carlos Zeferino ◽  
Eliane Regina de Oliveira Zambeli ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Dna Detection ◽  
Pap Smears ◽  
Adenocarcinoma In Situ ◽  
Histological Findings ◽  
Glandular Cells ◽  
Atypical Glandular Cells ◽  
Papillomavirus Dna

Clinical Utility of Adjunctive High-Risk Human Papillomavirus DNA Testing in Women With Papanicolaou Test Findings of Atypical Glandular Cells

2010 ◽  
Vol 134 (1) ◽  
pp. 103-108
Author(s):  
Chengquan Zhao ◽  
Anca Florea ◽  
R. Marshall Austin
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Cervical Intraepithelial Neoplasia ◽  
Pap Test ◽  
Intraepithelial Neoplasia ◽  
Adenocarcinoma In Situ ◽  
Test Results ◽  
Pap Tests

Abstract Context. Atypical glandular cell (AGC) Papanicolaou (Pap) test interpretations are challenging. Most biopsy findings are benign, but AGC results may also reflect highly significant noninvasive neoplastic and malignant histologic outcomes. High-risk human papillomavirus (hrHPV) test use with AGC Pap test results is evolving. Objective. To further evaluate the utility and limitations of hrHPV testing with AGC Pap tests. Design. Hospital records were searched for AGC Pap tests results from June 1, 2005, to August 31, 2007. Cases of AGC with hrHPV tests and histopathologic follow-up were included. Results. Of the 662 women with AGC Pap test results and follow-up analyzed, hrHPV results were available for 309 (46.7%) and were positive in 75 cases (24.3%). Among the 75 cases with hrHPV+ AGC results, 13 (17.3%) had cervical intraepithelial neoplasia grades 2/3, 10 (13.3%) had adenocarcinoma in situ, and 3 (4.0%) had cervical invasive adenocarcinoma, whereas for 234 women with hrHPV− results, 1 (0.4%) had cervical intraepithelial neoplasia grades 2/3, 1 (0.4%) had adenocarcinoma in situ, 1 each (0.4%) had cervical adenocarcinoma and ovarian carcinoma, and 8 (3.4%) had endometrial carcinoma. Conclusions. Positive hrHPV AGC results were most strongly associated with cervical intraepithelial neoplasia grades 2/3 and adenocarcinoma in situ in women younger than 50 years. Positive hrHPV AGC results were also present in all 3 cases of invasive cervical adenocarcinoma in women younger than 50 years. Of note, hrHPV− AGC results were present in 10 of 13 carcinomas (76.9%) detected after AGC Pap tests, all in women 40 years or older with endometrial adenocarcinomas (n  =  8), ovarian carcinoma (n  =  1), and cervical adenosquamous carcinoma in a woman (n  =  1) in her 50s. Testing for hrHPV after AGC Pap testing was most helpful in the detection of cervical intraepithelial neoplasia grades 2/3, adenocarcinoma in situ, and invasive cervical adenocarcinomas in women younger than 50 years.

Human Papillomavirus-specific Genotypes in Cervical Lesions of Women Referred for Smears With Atypical Glandular Cells or Adenocarcinoma In Situ

2009 ◽  
Vol 28 (3) ◽  
pp. 272-278 ◽  
Author(s):  
Silvia Helena Rabelo-Santos ◽  
Sophie Françoise Mauricette Derchain ◽  
Luísa Lina Villa ◽  
Maria Cecília Costa ◽  
Luis Otávio Zanatta Sarian ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Adenocarcinoma In Situ ◽  
Cervical Lesions ◽  
Glandular Cells ◽  
Atypical Glandular Cells

Cytological Diagnoses of Adenocarcinoma in situ of the Cervix: Common Misdiagnoses

2015 ◽  
Vol 59 (1) ◽  
pp. 91-96
Author(s):  
Shuxia Li ◽  
Dan Tian ◽  
Ying Li
Keyword(s):  
False Negative ◽  
Adenocarcinoma In Situ ◽  
Small Cell Neuroendocrine Carcinoma ◽  
Squamous Cells ◽  
Not Otherwise Specified ◽  
Glandular Cells ◽  
Atypical Glandular Cells ◽  
Intraepithelial Lesion ◽  
Intraepithelial Lesions

Objective: In routine screening, glandular lesions have high false-negative and false-positive rates. Our study sought to improve the cytological diagnoses and differentiation of glandular lesions. Study Design: A total of 24 cytology slides were retrospectively reviewed. Among these slides, 15 were confirmed via histology to have varying amounts of adenocarcinoma in situ (AIS) components. The other 9 cytology slides exhibited AIS mimics that had been originally considered to be AIS or atypical glandular cells (AGCs) and were selected for differentiation from AIS. Results: Overall, 12 of the 15 cytological slides exhibited significant atypical glandular features that met the criteria for AIS. However, the original cytological diagnoses included 2 cases of AIS, 3 AGCs-favor neoplasia (AGC-FN), 2 AGCs, 1 case that was not otherwise specified (AGC-NOS), 1 NILM (negative for intraepithelial lesion and malignancy), 3 HSILs (high-grade squamous intraepithelial lesions), and 2 ASC-Hs (atypical squamous cells, cannot exclude HSIL). The latter 9 cytological slides were subsequently histologically confirmed to be 2 metaplasias, 2 reactive lesions, 1 decidual change, 3 serous adenocarcinomas, and 1 SCNEC (small-cell neuroendocrine carcinoma). Conclusion: Cytological screening will be valuable for identifying glandular lesions as effectively as squamous lesions if the doubly high false rates can be decreased.

scholarly journals Human Papillomavirus Testing and Reporting Rates in 2012: Results of a College of American Pathologists National Survey

2014 ◽  
Vol 139 (6) ◽  
pp. 757-761 ◽  
Author(s):  
Chengquan Zhao ◽  
Ann T. Moriarty ◽  
Mohiedean Ghofrani ◽  
Mujtaba Husain ◽  
Rosemary H. Tambouret ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Proficiency Testing ◽  
Hpv Testing ◽  
Papanicolaou Test ◽  
Squamous Cells ◽  
Human Papillomavirus Testing ◽  
Glandular Cells ◽  
Atypical Glandular Cells ◽  
Reflex Testing ◽  
Roche Cobas

Context College of American Pathologists (CAP) surveys are used to establish national benchmarks for laboratory parameters. Objective To evaluate changes in laboratory human papillomavirus (HPV) testing patterns in laboratories incorporating HPV testing with Papanicolaou tests in 2012. Design Data were analyzed from the CAP HPV Supplemental Questionnaire distributed to 1771 laboratories participating in either CAP HPV or CAP Papanicolaou proficiency testing in 2013. Results A total of 1022 laboratories (58%) responded. There were more high-risk (HR) HPV tests performed per institution as compared to previous surveys. There were more HPV tests performed within an institution as compared to previous surveys. Hybrid Capture 2 (HC2) remains the most common method (42.4%, 239 of 564); Cervista and cobas methods are used in 37.2% (210 of 564) and 14.9% (84 of 564) of laboratories, respectively. Human papillomavirus testing is offered as a reflex test after a Papanicolaou test result of atypical squamous cells of undetermined significance (ASC-US) in 89.6% of laboratories (476 of 531); as a cotest for women aged 30 years and older in 60.3% (404 of 531); as reflex testing after atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) in 42.7% (320 of 531); and as reflex testing after atypical glandular cells (AGC) in 27.3% (145 of 531). The HPV-positive rates for ASC-US and ASC-H were similar in 2012 and 2006. Cervista (49.2%, 88 of 179) and Roche cobas (27.4%, 49 of 179) are the most common methods used for genotyping. Most laboratories use the CAP Human Papillomavirus for Cytology Program for proficiency testing. Conclusions There was an increase in annual volume of HR-HPV testing with a shift toward in-house HR-HPV testing. Genotyping volumes also increased. HC2 and Cervista are most commonly used, with an increasing volume of Roche cobas testing. The most common indication for HPV testing among all laboratories was ASC-US reflex testing, but an increase in HPV cotesting was observed. The data provide an update into persisting and newer trends in HPV testing practices.

High-risk human papillomavirus DNA testing: A marker for atypical glandular cells

2006 ◽  
Vol 34 (3) ◽  
pp. 235-239 ◽  
Author(s):  
Anjali Saqi ◽  
Prabodh K. Gupta ◽  
Maria Erroll ◽  
Anamaria Babiac ◽  
Dorothy Blackmun ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Dna Testing ◽  
Glandular Cells ◽  
Atypical Glandular Cells ◽  
Papillomavirus Dna
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