First Record of a B Chromosome in Polybia fastidiosuscula Saussure (Vespidae) and Investigation of Chromatin Composition Through Microsatellite Mapping

2020 ◽  
Vol 160 (11-12) ◽  
pp. 711-718
Author(s):  
Priscila Marchioro ◽  
Lucio A.O. Campos ◽  
Denilce M. Lopes
Keyword(s):  
Chromosome Number ◽  
First Record ◽  
B Chromosome ◽  
Base Pairs ◽  
Fluorochrome Staining ◽  
First Case ◽  
The Family ◽  
Microsatellite Mapping ◽  
Evolution Of Genomes

The characterization of karyotypes is an important aspect in understanding the structure and evolution of genomes. Polybia is a genus of social wasps of the family Vespidae. This genus has 58 species, but for only 8 of these chromosome number and morphology have been reported in the literature. The aim of this study was to describe and characterize the Polybia fastidiosuscula Saussure karyotype, presenting the first case of a B chromosome in Vespidae. In addition, we investigated the chromatin composition of this species through C-banding, base-specific fluorochrome staining, and physical mapping of 7 microsatellites and 18S rDNA. Four colonies of P. fastidiosuscula from Minas Gerais and Paraná states, Brazil, were analyzed. The chromosome number identified was 2n = 34, and 2 colonies presented a B chromosome. We characterized the chromatin composition of this species, analyzing the existence of different microsatellite-rich heterochromatic regions which are also enriched with AT or GC base pairs. We suggest an intraspecific origin of the B chromosome based on the homology of the heterochromatic composition with A chromosomes and also verify that the TTAGG and TCAGG sequences are not telomeric, but only microsatellites that occur in the centromeres of most chromosomes, as well as GAG and CGG.

scholarly journals Axanthism in amphibians: A review and the first record in the widespread toad of the Bufotes viridis complex (Anura: Bufonidae)

2020 ◽  
Vol 144 (2) ◽  
Author(s):  
Daniel Jablonski ◽  
Andrej Alena ◽  
Petr Vlček ◽  
David Jandzik
Keyword(s):  
First Record ◽  
Pigment Cells ◽  
Extensive Review ◽  
First Case ◽  
Green Toad ◽  
The Family ◽  
Color Aberration

Axanthism in amphibians is a relatively rare color aberration reported less often than leucism or albinism. It is caused by lack of specific types of pigment cells in the skin, namely xanthophores, erythrophores, and iridophores. Here, we present the first case of occurrence of this aberration in a widely distributed toad of the green toad (Bufotes viridis) complex and provide an extensive review of axanthism occurrence in amphibians. So far it has been reported in more than 20 species from nine families of amphibians with the highest occurrence in the family Ranidae.

First Record on Sex Chromosomes in a Species of the Family Cynodontidae: Cynodon gibbus (Agassiz, 1829)

2020 ◽  
Vol 160 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Vanessa S. Pinheiro Figliuolo ◽  
Leonardo Goll ◽  
Patrik Ferreira Viana ◽  
Eliana Feldberg ◽  
Maria C. Gross
Keyword(s):  
Chromosome Number ◽  
Repetitive Dna ◽  
Sex Chromosomes ◽  
First Record ◽  
Diploid Chromosome Number ◽  
Fish Family ◽  
Female Heterogamety ◽  
The Family

The fish family Cynodontidae belongs to the superfamily Curimatoidea, together with the Hemiodontidae, Serrasalmidae, Parodontidae, Prochilodontidae, Chilodontidae, Curimatidae, and Anostomidae. The majority of the species of this superfamily that have been analyzed to date have a diploid chromosome number of 2n = 54. Differentiated sex chromosomes (with female heterogamety) have been observed only in the Prochilodontidae, Parodontidae, and Anostomidae. The present study provides the first description of differentiated sex chromosomes in the cynodontid species Cynodon gibbus, which has a ZZ/ZW system, and shows that repetitive DNA has played a fundamental role in the differentiation of these sex chromosomes.

scholarly journals Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus

2005 ◽  
Vol 28 (3) ◽  
pp. 390-393 ◽  
Author(s):  
Alayne Magalhães Trindade Domingues ◽  
Ana Maria Waldschmidt ◽  
Sintia Emmanuelle Andrade ◽  
Vanderly Andrade-Souza ◽  
Rogério Marco de Oliveira Alves ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Fluorochrome Staining ◽  
C Banding ◽  
Trigona Fulviventris

scholarly journals Characterization of the geographical distribution pattern of the family Limacinidae Gray, 1840 (Mollusca - Gastropoda) in the waters of Northeastern of Brazil

2014 ◽  
Vol 14 (2) ◽  
Author(s):  
Valdeni Soares de Oliveira Koblitz ◽  
Maria Eduarda de L. Larrazábal
Keyword(s):  
Geographical Distribution ◽  
Mesh Size ◽  
First Record ◽  
Program Assessment ◽  
Ecological Aspects ◽  
The Family ◽  
First Time ◽  
Plastic Containers ◽  
Geographical Distribution Pattern

The geographical distribution of the family Limacinidae in the Northeastern coast of Brazil was analyzed by taking into account the most relevant ecological aspects, aiming to increase the knowledge about the Family. The material was collected during the 4th Oceanographic Expedition of the REVIZEE program (Assessment of the Sustainable Potential of Living Resources in the Exclusive Economic Zone - Avaliação do Potencial Sustentável de Recursos Vivos da Zona Econômica Exclusiva), in the months from September to December 2000. The studied area is located between 00°46’45”N and 13°53’45”S and between 29°15’40”W and 39°49’42”W, where six trips were performed, totaling 123 stations. The samples were collected using a bongo net (300- and 500-µm mesh size and net mouths of 60 cm in diameter) coupled with a digital flowmeter in oblique hauls from a depth of 0 to 200 m. For this study, the organisms retained in the 300-µm mesh were considered. On board the ship, the samples were placed in plastic containers, labeled, and fixed in 4% formaldehyde buffered with sodium tetraborate. In the laboratory, the samples were analyzed on a “Bogorov” tray under a binocular stereomicroscope. A total of 5655 individuals of the family Limacinidae were examined and were distributed as follows: 3 genera and 5 species. Representatives of the family Limacinidae were observed at high temperatures and salinities and were thus characterized as tropical and euhaline.Heliconoides inflatus was the species with the greatest abundance and frequency. Limacina lesueurii proved to be a rare, infrequently observed species. Limacina trochiformis andL. bulimoides were not abundant or frequent.Limacina lesueurii and L. bulimoides were recorded in neritic waters for the first time; this was also the first record ofL. lesueurii in the waters of Northeastern Brazil.Limacina trochiformis and L. bulimoidesexhibited wide distributions, although they were neither very frequently observed nor abundant. The distribution of Thielea helicoideswas restricted to oceanic waters. A correlation between Limacina bulimoides, Heliconoides inflatus, Thielea helicoides, and L. lesueurii was observed because they coexisted in the same niche.

scholarly journals Karyotypic characterization of Prochilodus mariae, Semaprochilodus kneri and S. laticeps(Teleostei: Prochilodontidae) from Caicara del Orinoco, Venezuela

2003 ◽  
Vol 1 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Claudio Oliveira ◽  
Mauro Nirchio ◽  
Ángel Granado ◽  
Sara Levy
Keyword(s):  
Chromosome Number ◽  
Chromosome Pair ◽  
Nucleolus Organizer ◽  
Single Chromosome ◽  
Nucleolus Organizer Regions ◽  
The Family ◽  
Structural Chromosome ◽  
Almost All ◽  
Freshwater Environments

Fish of the family Prochilodontidae are considered one of the most important components of commercial and subsistence fishery in freshwater environments in South America. This family consists of 21 species and three genera. In the present study, the karyotypes of Prochilodus mariae, Semaprochilodus kneri, and S. laticeps from Caicara del Orinoco, Bolivar State, Venezuela were studied. The species P. mariae, S. kneri and S. laticeps exhibited 2n=54 chromosomes (40 metacentric and 14 submetacentric), a single chromosome pair with nucleolus organizer regions, and a large amount of heterochromatin found at centromeric and pericentromeric positions in almost all chromosomes. The P. mariae specimens studied displayed 0 to 3 supernumerary microchromosomes. The data obtained here confirm the conservative nature of the chromosome number and morphology of Prochilodontidae and reinforce the hypothesis that small structural chromosome rearrangements were the main cause of the karyotypic diversification seen in this group.

Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran

Biologia ◽  
2013 ◽  
Vol 68 (5) ◽  
Author(s):  
Mahnaz Hassani-Kakhki ◽  
Javad Karimi ◽  
Ebrahim Shokoohi
Keyword(s):  
First Record ◽  
Morphological Characterization ◽  
The Body ◽  
Phylogenetic Position ◽  
Rdna Genes ◽  
Pristionchus Pacificus ◽  
The Family ◽  
Identification Measurements ◽  
Morphometric Characterization

AbstractDuring a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69–0.83 mm), the stoma (6–10 μm) bearing a dorsal tooth and a conical elongated tail (146–220 μm). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.

First record of albinism in spiny rats of genus Proechimys (Rodentia: Echimyidae) from Western Amazon

Mammalia ◽  
2020 ◽  
Vol 84 (6) ◽  
pp. 605-609
Author(s):  
Jeronymo Dalapicolla ◽  
Paulo Ricardo de Oliveira Roth ◽  
Alexandre Reis Percequillo
Keyword(s):  
Natural Populations ◽  
Rare Condition ◽  
First Record ◽  
Small Population ◽  
Mitochondrial Marker ◽  
Base Pairs ◽  
The Family ◽  
Population Sizes ◽  
Albino Phenotype ◽  
Porto Velho

AbstractAlbinism is a rare condition in natural populations. One of the factors that would lead to higher allele frequency for the albino phenotype is bottleneck events, causing small population sizes and loss of genetic diversity. Here, we report the first record of albinism in one specimen of Proechimys gardneri from Porto Velho, Rondônia, Brazil. This is also the first record of albinism for the genus and for the family Echimyidae. We identified the specimen through morphology and confirmed the identification by 801 base pairs (bp) of mitochondrial marker cytochrome b (Cyt b). We also discuss the selection against albino phenotype in natural populations.

Morphological and phylogenetic characterization of a novel Unicauda species, infecting the kidney of Astyanax altiparanae (Teleostei: Characidae) in Brazil

2018 ◽  
Vol 63 (3) ◽  
pp. 495-503 ◽  
Author(s):  
Letícia P. Vidal ◽  
Douglas McIntosh ◽  
José L. Luque
Keyword(s):  
Mature Spore ◽  
First Record ◽  
Phylogenetic Characterization ◽  
The Family ◽  
Parasitological Survey ◽  
A New Species ◽  
Process Characteristic ◽  
Unequal Length ◽  
Occurrence Of Species

Abstract During a parasitological survey of Astyanax altiparanae captured in the Mogi Guaçú river, Pirassununga, state of São Paulo, Brazil, a new species of myxozoa was observed infecting the kidney of 30 of 42 specimens. Plasmodia of the new myxozoa, referred to herein as Unicauda whippsi n. sp. were spherical and varied from 450 to 1000 μm in length. The mature spore was elongated and measured 11 ± 1.2 (10–12) μm long by 5.4 ± 0.6 (4.7–7.2) μm wide. Polar capsules of unequal length with the filament coiled 7–9 times and a unique caudal process characteristic of the genus Unicauda, were recorded. Phylogenetic analysis demonstrated that U. whippsi n. sp. clustered with members of the genus Unicauda and Myxobolus, confirming it’s placement within the family Myxobolidae. This is the first record of a species of Unicauda infecting characiform fish, as well as the first registered occurrence of species of this genus in South America.

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

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