Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran

Biologia ◽  
2013 ◽  
Vol 68 (5) ◽  
Author(s):  
Mahnaz Hassani-Kakhki ◽  
Javad Karimi ◽  
Ebrahim Shokoohi
Keyword(s):  
First Record ◽  
Morphological Characterization ◽  
The Body ◽  
Phylogenetic Position ◽  
Rdna Genes ◽  
Pristionchus Pacificus ◽  
The Family ◽  
Identification Measurements ◽  
Morphometric Characterization

AbstractDuring a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69–0.83 mm), the stoma (6–10 μm) bearing a dorsal tooth and a conical elongated tail (146–220 μm). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.

Polymorphism and inconsistencies in the taxonomy of Diplodinium anisacanthum da Cunha, 1914 (Ciliophora, Entodiniomorphida, Ophryoscolecidae) and taxonomic notes on the genus Diplodinium Schuberg, 1888

Zootaxa ◽  
2017 ◽  
Vol 4306 (2) ◽  
pp. 249 ◽  
Author(s):  
FRANCIANE CEDROLA ◽  
ROBERTO JÚNIO PEDROSO DIAS ◽  
ISABEL MARTINELE ◽  
MARTA D’AGOSTO
Keyword(s):  
Morphometric Analysis ◽  
Single Species ◽  
Distinct Species ◽  
Molecular Data ◽  
Morphological Characterization ◽  
The Body ◽  
General Morphology ◽  
Polymorphic Species ◽  
Morphometric Characterization

The genus Diplodinium includes 31 species of ophryoscolecid ciliates with two retractable ciliary zones in the anterior end of the body, with no skeletal plates, inclined macronucleus and two contractile vacuoles. They have a broad geographical distribution and low host specificity. The genus has several taxonomic inconsistencies, mainly due to polymorphism of most species. Diplodinium anisacanthum is a case of taxonomic inconsistency in the genus. It presents polymorphism in posterior end of the body, so it is considered a polymorphic species or seven distinct species. This study presents the morphological characterization of six D. anisacanthum morphotypes based on general morphology, morphometric analysis and oral infraciliature characterization and taxonomic notes on the genus Diplodinium. The morphological and morphometric characterization of D. anisacanthum showed great similiarities among morphotypes suggesting that these ciliates constitute a single species with polymorphisms. A brief review conducted highlights the inconsistencies in the taxonomy of the genus Diplodinium and demonstrates the need for recent studies with representatives of the genus. There is no information on how polymorphism of Diplodinium anisacanthum morphotypes correlates to molecular data, since there are only two sequences of this species available in the GenBank database. 

scholarly journals First record of Palombitrema triangulum (Suriano, 1981) Suriano, 1997 (Monogenea: Dactylogyridae) from freshwater fishes in Brazil

2015 ◽  
Vol 75 (1) ◽  
pp. 229-233
Author(s):  
VD. Abdallah ◽  
RK. Azevedo ◽  
RJ. Silva
Keyword(s):  
Nasal Cavity ◽  
Body Surface ◽  
First Record ◽  
Freshwater Fishes ◽  
Morphological Characterization ◽  
The Body ◽  
Native Fish ◽  
Morphological Differences ◽  
First Time

This study reports for the first time the monogenean Palombitrema triangulum Suriano (1981) in freshwater fishes from Brazil, highlighting new sites of infection for this helminth and some morphological differences. Monogeneans were collected on the body surface, gills and nasal cavity of two native fish species from Brazil, Cyphocharax modestus (Fernández-Yépez, 1948) and Cyphocharax nagelii (Steindachner, 1881). A brief morphological characterization of this species is presented.

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Morphological Characterization ◽  
Test Method ◽  
Analysis Method ◽  
Nitrogen Base ◽  
Rdna Genes ◽  
Antibacterial Screening ◽  
Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

Morphological characterization of Trichotylenchus gorganiensis n. sp. (Nematoda: Dolichodoridae) and photomicrographs of several other species of the subfamily Telotylenchinae Siddiqi, 1960

Zootaxa ◽  
2018 ◽  
Vol 4526 (4) ◽  
pp. 447
Author(s):  
REZA GHADERI ◽  
AKBAR KAREGAR ◽  
ESMAEIL MIRAEIZ
Keyword(s):  
New Species ◽  
Morphological Characterization ◽  
The Body ◽  
Morphometric Data ◽  
Tail Region ◽  
Body Contour ◽  
Closely Related Species ◽  
Pharyngeal Bulb ◽  
Anal Sac

Trichotylenchus gorganiensis n. sp. is described and illustrated based on morphological and morphometric data. The new species is characterized by its 760–1073 µm long body, conoid-rounded lip region continuous with the body contour and bearing 5–7 fine striae, 22.0–24.5 µm long stylet, basal pharyngeal bulb offset or slightly overlapping intestine, post-anal sac extending 50–73 % of the tail region, and cylindrical or subclavate tail with a striated terminus. Differences of the new species from the closely related species T. astriatus, T. astriatoides, T. changlingensis and T. papyrus are discussed. Photomicrographs and several taxonomic notes on 13 other species of Telotylenchinae, collected from Iran, are provided. 

scholarly journals Genetic and Morphological Characterization of Mangifera indica L. Growing in Egypt

HortScience ◽  
2018 ◽  
Vol 53 (9) ◽  
pp. 1266-1270 ◽  
Author(s):  
Nader R. Abdelsalam ◽  
Hayssam M. Ali ◽  
Mohamed Z.M. Salem ◽  
Elsayed G. Ibrahem ◽  
Mohamed S. Elshikh
Keyword(s):  
Genetic Diversity ◽  
Mangifera Indica ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Molecular Techniques ◽  
Germplasm Management ◽  
Fruit Crops ◽  
The Family ◽  
High Diversity

Mango (Mangifera indica L.) is a fruit crops belong to the family Anacardiaceae and is the oldest cultivated tree worldwide. Cultivars maintained in Egypt have not been investigated previously. Mango was first brought to Egypt from South Asia. Morphological and molecular techniques were used to identify the genetic diversity within 28 mango cultivars. SSR and EST-SSR were used for optimizing germplasm management of mango cultivars. Significant variations were observed in morphological characteristics and genetic polymorphism, as they ranged from 0.71% to 100%. High diversity was confirmed as a pattern of morphological and genotypes data. Data from the present study may be used to calculate the mango relationship and diversity currently grown in Egypt.

scholarly journals A Novel Genotype and First Record of Trypanosoma lainsoni in Argentina

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 731
Author(s):  
Anahí G. Díaz ◽  
Paula G. Ragone ◽  
Fanny Rusman ◽  
Noelia Floridia-Yapur ◽  
Rubén M. Barquez ◽  
...  
Keyword(s):  
Host Range ◽  
First Record ◽  
Morphological Characterization ◽  
Pairwise Identity ◽  
Wild Mammals ◽  
Transmission Electron ◽  
Chaco Region ◽  
Veterinary Importance ◽  
First Time

Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the Trypanosoma genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with Trypanosoma lainsoni, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of T. lainsoni and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of T. lainsoni was expanded (Leopardus geoffroyi, Carenivora, Felidae; and Calomys sp., Rodentia, Cricetidae), showing a wide host range for this species.

scholarly journals Morphometric Characterization of the Lidia Cattle Breed

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1180
Author(s):  
Juan Manuel Lomillos ◽  
Marta E. Alonso
Keyword(s):  
Cattle Breed ◽  
Internal Variability ◽  
Morphological Characterization ◽  
Relative Depth ◽  
Sexually Dimorphic ◽  
Muscular Development ◽  
Morphological Differences ◽  
Positive Correlations ◽  
Morphometric Characterization

Morphometric studies in Lidia cattle are scarce due to the challenges of handling and approaching this breed of cattle. For this reason, the first morphological characterization of the Lidia breed was carried out using photogrammetry. In the present work, 264 adult individuals (184 males and 80 females), belonging to 21 different herds, were studied. A total of 20 morphological measurements and five indexes were determined in every individual. There were many positive correlations between the measures, giving the model great morphostructural harmony. Considerable internal variability of the studied parameters was observed. This breed reflected significant sexually dimorphic features and internal morphological differences between the different genetic lines of the breed. Lidia cattle are small and mostly have a sub-concave profile (58.4% males and 69.7% females). The male proportionality indexes and the relative depth of the thorax indicated great muscular development of the anterior third and high thoracic capacity. The phaneroptic information describes a mostly black animal with black mucous and hooves and an outstanding development of the dewlaps and the tail in the males.

Phylogenetic position of Sphaerospora testicularis and Latyspora scomberomori n. gen. n. sp. (Myxozoa) within the marine urinary clade

Parasitology ◽  
2010 ◽  
Vol 138 (3) ◽  
pp. 381-393 ◽  
Author(s):  
PAVLA BARTOŠOVÁ ◽  
MARK A. FREEMAN ◽  
HIROSHI YOKOYAMA ◽  
MONICA CAFFARA ◽  
IVAN FIALA
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Dicentrarchus Labrax ◽  
Sensu Stricto ◽  
Phylogenetic Position ◽  
Species Cluster ◽  
The Family ◽  
Sutural Line ◽  
Taxonomic Changes

SUMMARYAn amendment of the family Sinuolineidae (Myxosporea) is proposed in order to include a newly described genus Latyspora n. gen. The type species Latyspora scomberomori n. gen. n. sp. is a coelozoic parasite in the kidney tubules of Scomberomorus guttatus. In addition to the morphological and molecular characterization of L. scomberomori n. gen. n. sp., we also present novel SSU rDNA data on Sphaerospora testicularis, a serious parasite of Dicentrarchus labrax. Performed phylogenetic analyses revealed that both species cluster within the marine urinary clade encompassing the representatives with a shared insertion within their V4 SSU rRNA region and grouping according to the shape of their spores’ sutural line and their similar tissue tropism in the host. Sphaerospora testicularis is the closest relative to Parvicapsula minibicornis within the Parvicapsula subclade and L. scomberomori n. gen. n. sp. is the basal species of the Zschokkella subclade. The phylogenetic position of S. testicularis, outwith the basal Sphaerospora sensu stricto clade, and its morphology suggest it being a non-typical Sphaerospora. The sequence data provided on S. testicularis can help in future revisions of the strongly polyphyletic genus Sphaerospora. We recommend re-sequencing of several sphaerosporids as an essential step before such taxonomic changes are accomplished.

scholarly journals First record of a reticulated toadfish, Arothron reticularis (Tetraodontiformes: Tetraodontidae), in Korea

2020 ◽  
Vol 23 (1) ◽  
Author(s):  
Chung-Bae Kang ◽  
Sang-Hwa Lee ◽  
Tae-Sik Yu ◽  
Hae-Rim Lee ◽  
Kyeong-Ho Han
Keyword(s):  
Maximum Likelihood ◽  
Coastal Waters ◽  
Morphological Characteristics ◽  
First Record ◽  
The Body ◽  
Single Specimen ◽  
White Line ◽  
Maximum Likelihood Tree ◽  
White Spots ◽  
The Family

AbstractA single specimen of Arothron reticularis (398 mm in total length), belonging to the family Tetraodontidae, was collected in the coastal waters off Hansan island using a set net in May 2019. The morphological characteristics of the specimen are as follows: large eyes and mouth, white spots on the body, and a white line surrounding the eyes in an annular shape. A result of maximum likelihood tree showed that A. reticularis is related to A. hispidus (93.6%). We proposed the Korean name “Geu-murl-mu-nui-kkeo-kkeurl-bok,” in accordance with the characteristics of the specimen.

scholarly journals New multipodomerous appendages of stem-group euarthropods from the Cambrian (Stage 4) Guanshan Konservat-Lagerstätte

2021 ◽  
Vol 8 (11) ◽  
Author(s):  
De-guang Jiao ◽  
Stephen Pates ◽  
Rudy Lerosey-Aubril ◽  
Javier Ortega-Hernández ◽  
Jie Yang ◽  
...  
Keyword(s):  
South China ◽  
First Record ◽  
Phylogenetic Position ◽  
Ecological History ◽  
Stem Group ◽  
Stage 4 ◽  
The Family ◽  
Guanshan Biota ◽  
History Of ◽  
Element Bearing

Stem-group euarthropods are important for understanding the early evolutionary and ecological history of the most species-rich animal phylum on Earth. Of particular interest are fossil taxa that occupy a phylogenetic position immediately crownwards of radiodonts, for this part of the euarthropod tree is associated with the appearance of several morphological features that characterize extant members of the group. Here, we report two new euarthropods from the Cambrian Stage 4 Guanshan Biota of South China. The fuxianhuiid Alacaris ? sp. is represented by isolated appendages composed of a gnathobasic protopodite and an endite-bearing endopod of at least 20 podomeres. This material represents the youngest occurrence of the family Chengjiangocarididae, and its first record outside the Chengjiang and Xiaoshiba biotas. We also describe Lihuacaris ferox gen. et sp. nov. based on well-preserved and robust isolated appendages. Lihuacaris ferox exhibits an atypical combination of characters including an enlarged rectangular base, 11 endite-bearing podomeres and a hypertrophied distal element bearing 8–10 curved spines. Alacaris ? sp. appendages display adaptations for macrophagy. Lihuacaris ferox appendages resemble the frontal appendages of radiodonts, as well as the post-oral endopods of chengjiangocaridid fuxianhuids and other deuteropods with well-documented raptorial/predatory habits. Lihuacaris ferox contributes towards the record of endemic biodiversity in the Guanshan Biota.

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