Validation of Fenoterol to Study β2-Adrenoceptor Function in the Rat Urinary Bladder
Fenoterol is a β<sub>2</sub>-adrenoceptor (AR)-selective agonist that is commonly used to investigate relaxation responses mediated by β<sub>2</sub>-AR in smooth muscle preparations. Some data have questioned this because fenoterol had low potency in the rat urinary bladder when a muscarinic agonist was used as a pre-contraction agent and because some investigators proposed that fenoterol may act in part via β<sub>3</sub>-AR. We designed the present study to investigate whether fenoterol is a proper pharmacological tool to study β<sub>2</sub>-AR-mediated relaxation responses in the rat urinary bladder. Firstly, we have compared the effect of pre-contraction agents on fenoterol potency and found that fenoterol potency was about 1.5 log units greater against KCl than carbachol (pEC<sub>50</sub> 7.19 ± 0.66 and 5.62 ± 1.09 of KCl and of carbachol, respectively). To test the selectivity of fenoterol, we have determined the effects of the β<sub>2</sub>-AR antagonist ICI 118,551 and the β<sub>3</sub>-AR antagonist L 748,337 on relaxation responses to fenoterol. While 300 nM L 748,337 had little effect on the potency of fenoterol (pEC<sub>50</sub> 6.56 ± 0.25 and 6.33 ± 0.61 in the absence and presence of L 748,337, respectively), the relaxation curve for fenoterol was right-shifted in the presence 300 nM ICI 118,551 (pEC<sub>50</sub> 5.03 ± 0.18). Thus, we conclude that fenoterol is a proper pharmacological tool to assess β<sub>2</sub>-AR-mediated responses in the rat urinary bladder and most likely in other smooth-muscle preparations containing multiple subtypes of the β-AR.
Smooth muscle and parasympathetic nerve terminals in the rat urinary bladder have different subtypes of α1
adrenoceptors
