scholarly journals Uptake of norepinephrine in an isolated artery from normotensive humans.

Hypertension ◽  
1991 ◽  
Vol 18 (3) ◽  
pp. 348-354
Author(s):  
D G Wyse
Keyword(s):  
Isolated Artery

Effects of hypertensive plasma on the responses of an isolated artery preparation to noradrenaline

1976 ◽  
Vol 10 (2) ◽  
pp. 268-274 ◽  
Author(s):  
D. S. BLOOM ◽  
M. G. STEIN ◽  
C. ROSENDORFF
Keyword(s):  
Isolated Artery

scholarly journals Assessment of Endothelin-A Receptor Expression in Subcutaneous and Orthotopic Thyroid Carcinoma Xenografts in Vivo Employing Optical Imaging Methods

Endocrinology ◽  
2012 ◽  
Vol 153 (6) ◽  
pp. 2907-2918 ◽  
Author(s):  
Katrin Büther ◽  
Matthijs G. Compeer ◽  
Jo G. R. De Mey ◽  
Otmar Schober ◽  
Michael Schäfers ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Optical Imaging ◽  
Near Infrared ◽  
Functional Performance ◽  
Thyroid Tumor ◽  
Receptor Expression ◽  
Imaging Methods ◽  
Carcinoma Cell Lines ◽  
Isolated Artery

Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ETAR) in murine models of thyroid carcinoma using optical imaging methods. A recently developed near-infrared fluorescent tracer was first assessed in isolated artery preparations for its functional performance in comparison with known ETAR antagonists BQ123 and PD156707. Before evaluation of the tracer in vivo, different thyroid carcinoma cell lines were characterized with respect to their ET receptor expression by RT-PCR and autoradiography. In vivo, sc and orthotopic papillary thyroid tumor xenografts were clearly visualized by fluorescence reflectance imaging and fluorescence-mediated tomography up to 48 h after injection of the tracer. Binding specificity of the probe was demonstrated by predosing with PD156707 as a competing inhibitor. In conclusion, optical imaging with a fluorescent ETAR tracer allows the noninvasive imaging of tumor-associated ETAR expression in vivo. In the future, this technique may help surgeons to evaluate lesion dimensions in intraoperative settings (e.g. thyroidectomy).

Effect of hydrogen ion changes on vascular resistance in isolated artery segments

1964 ◽  
Vol 207 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Oliver Carrier ◽  
Meredith Cowsert ◽  
John Hancock ◽  
Arthur C. Guyton
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Sulfuric Acid ◽  
Nitric Acid ◽  
Perfusion Pressure ◽  
Control Level ◽  
Linear Increase ◽  
Hydrogen Ion ◽  
Isolated Artery ◽  
Ph Range

Isolated arterial segments, 1 cm in length and 0.5–1.0 mm in diameter, were perfused with Tyrode's solution titrated to various levels of pH. Po2, Pco2, and temperature were held at physiological levels; the perfusion pressure was held at 100 mm Hg, and flow was measured by a drop counter. There was a linear increase in flow as the pH was decreased from 7.4, 0.05 units at a time, with an increase of 87% obtained at pH 7.15. As the pH was further decreased, the flow dropped until at pH 6.8 it leveled off slightly above control level. When the pH was raised, there was an initial 35% decrease in flow by the time pH 7.50 was reached, followed by an increase, reaching 50% above control level at 7.65. At still higher pHs a precipitous decrease in conductance occurred, flow leveling off slightly below control level at pH 7.80. Consistent results were obtained on 45 vessels using Tyrode's solution titrated to the desired pH with lactic acid, hydrochloric acid, acetic acid, sulfuric acid, nitric acid, sodium hydroxides, or sodium bicarbonate. These results indicate that vessels have a very narrow pH range in which they maintain physiological tone.

Formation of adenosine nucleotide derivatives in isolated hog carotid artery strips

1977 ◽  
Vol 233 (2) ◽  
pp. H299-H304
Author(s):  
G. L. Van Harn ◽  
R. Rubio ◽  
R. M. Berne
Keyword(s):  
Carotid Artery ◽  
Specific Activity ◽  
Purine Derivatives ◽  
Twofold Increase ◽  
Threefold Increase ◽  
Isolated Artery ◽  
Adenosine Nucleotide

Media strips of hog carotid artery formed hypoxanthine and inosine during incubation under conditions of normoxia (95% O2, 5% CO2). During anoxia (95% N2, 5% CO2), hypoxanthine increased fivefold and inosine twofold. Stimulation with 124 mM K+ resulted in a twofold increase in hypoxanthine and a threefold increase in inosine. Concurrent with the increase in the concentrations of purine derivatives was a decrease in tissue ATP. Although significant amounts of adenosine were not detected in the medium of incubating artery strips, the following evidence suggests adenosine was formed and rapidly deaminated to inosine: 1) Exogenous adenosine added to the medium of incubating strips was rapidly deaminated to inosine. 2) Exogenous 5'-AMP concentration decreased, whereas adenosine and, subsequently, inosine levels increased during incubation of artery strips. The reaction was specific for 5'-AMP and the data suggest that AMP is dephosphorylated to adenosine. 3) The specific activity of exogenous [U-14C]adenosine added to the medium of incubated strips decreased after 15 min. It is concluded that adenosine is formed in isolated artery strips but is rapidly deaminated to inosine.

scholarly journals Role of store-operated Ca2+ entry in adenosine-induced vasodilatation of rat small mesenteric artery

2009 ◽  
Vol 297 (1) ◽  
pp. H347-H354 ◽  
Author(s):  
Shengpeng Wang ◽  
Yan Zhang ◽  
W. Gil Wier ◽  
Xiaojiang Yu ◽  
Ming Zhao ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
Protective Role ◽  
Dependent Manner ◽  
Resistance Arteries ◽  
Concentration Dependent Manner ◽  
Scanning Confocal Microscopy ◽  
Store Depletion ◽  
Intracellular Ca ◽  
Isolated Artery

Store-operated Ca2+ entry (SOCE) has recently been proposed to contribute to Ca2+ influx in vascular smooth muscle cells (VSMCs). Adenosine is known for its protective role against hypoxia and ischemia by increasing nutrient and oxygen supply through vasodilation. This study was designed to examine the hypothesis that SOCE have a functional role in adenosine-induced vasodilation. Small mesenteric resistance arteries and mesenteric VSMCs were obtained from rats. Isometric tensions of isolated artery rings were measured by a sensitive myograph system. Laser-scanning confocal microscopy was used to determine the intracellular Ca2+ concentration of fluo 3-loaded VSMCs. Adenosine (0.1–100 μM) relaxed artery rings that were precontracted by phenylephrine in a concentration-dependent manner. In cultured mesenteric VSMCs, passive store depletion by thapsigargin and active store depletion by phenylephrine both induced Ca2+ influx due to SOCE. Adenosine inhibited SOCE-mediated increases in cytosolic Ca2+ levels evoked by the emptying of the stores. In isolated artery rings, adenosine inhibited SOCE-induced contractions due to store depletion. A2A receptor antagonism with SCH-58261 and adenylate cyclase inhibition with SQ-22536 largely attenuated adenosine responses. The cAMP analog 8-bromo-cAMP mimicked the effects of adenosine on SOCE. Our results indicate a novel mechanism of vasodilatation by adenosine that involves regulation of SOCE through the cAMP signaling pathway due to activation of adenosine A2A receptors.

scholarly journals Microsurgical anatomy of the arterial compartment of the cavernous sinus: analysis of 24 cavernous sinus

2005 ◽  
Vol 63 (2a) ◽  
pp. 259-264 ◽  
Author(s):  
Gustavo Isolan ◽  
Evandro de Oliveira ◽  
João Paulo Mattos
Keyword(s):  
Carotid Artery ◽  
Internal Carotid Artery ◽  
Cavernous Sinus ◽  
Internal Carotid ◽  
Sella Turcica ◽  
Microsurgical Anatomy ◽  
Isolated Artery ◽  
Anatomy Knowledge ◽  
Tentorial Artery ◽  
Meningohypophyseal Trunk

The cavernous sinus is a complex compartment situated in both sides of the sella turcica, being its microsurgical anatomy knowledge of fundamental importance when consider to approach surgically. We studied the arterial microanatomy of 24 cavernous sinus at the microsurgical laboratory, considering that in all the internal carotid artery were filled with colored latex. The meningohypophyseal trunk was present in 18 cases (75%) with its origin in intracavernous portion of the internal carotid artery. In relation to the 18 presented cases with meningohypophyseal trunk, 14 (77.7%) had a trifurcate and 4 (23.3%) had a bifurcate pattern. The tentorial artery was present in all. Its origin was observed, arising from the meningohypophyseal trunk in 17 (70.8%) and as an isolated artery in some extension of the intracavernous portion in 7 (29.1%). An accessory tentorial artery was found in one specimen. The dorsal meningeal artery was present in 22 cases (91.6%). Its origin was in the meningohypophyseal trunk in 17 cases (77.2%), arising from internal carotid artery in 4 cases (18.1%) and from inferior hypophyseal artery in one case (4.1%).The inferior hypophyseal artery was present in all cases, having its origin at the meningohypophyseal trunk in 16 cases (66.6%). In the remaining 8 cases (33.3%) the artery was found arising alone from the intracavernous portion also. The artery of the inferior cavernous sinus or inferolateral trunk was present in all cases and had its origin from internal carotid artery in its intracavernous segment. The McConnell's artery was not found in any cavernous sinus.

EFFECT OF VERAPAMIL ON THE CONTRACTILE PROPERTIES OF LEAD—TREATED ISOLATED ARTERY

Abstracts ◽  
1978 ◽  
pp. 41
Author(s):  
F. Piccinini ◽  
A. Rozza ◽  
E. Lanza ◽  
N.C. Chiari ◽  
L. Favalli
Keyword(s):  
Contractile Properties ◽  
Isolated Artery
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