scholarly journals Glucocorticoids Inhibit Superoxide Anion Production and p22 Phox mRNA Expression in Human Aortic Smooth Muscle Cells

Hypertension ◽  
1998 ◽  
Vol 32 (6) ◽  
pp. 1083-1088 ◽  
Author(s):  
Takeshi Marumo ◽  
Valérie B. Schini-Kerth ◽  
Ralf P. Brandes ◽  
Rudi Busse
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
Superoxide Anion ◽  
Muscle Cells ◽  
Superoxide Anion Production ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Anion Production ◽  
P22 Phox

Superoxide anion impairs contractility in cultured aortic smooth muscle cells

2002 ◽  
Vol 283 (1) ◽  
pp. H382-H390 ◽  
Author(s):  
Chiwaka Kimura ◽  
Wei Cheng ◽  
Kazunari Hisadome ◽  
Yi-Ping Wang ◽  
Tetsuya Koyama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Superoxide Anion ◽  
Collagen Gel ◽  
Muscle Cells ◽  
Smooth Muscle Contraction ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
A 23187 ◽  
High K

We examined the effects of superoxide anion (O[Formula: see text]) generated by xanthine plus xanthine oxidase (X/XO) on the intracellular Ca2+ concentration ([Ca2+]i) and muscle contractility in cultured bovine aortic smooth muscle cells (BASMC). Cells were grown on collagen-coated dish for the measurement of [Ca2+]i. Pretreatment with X/XO inhibited ATP-induced Ca2+ transient and Ca2+release-activated Ca2+ entry (CRAC) after thapsigargin-induced store depletion, both of which were reversed by superoxide dismutase (SOD). In contrast, Ca2+ transients induced by high-K+ solution and Ca2+ ionophore A-23187 were not affected by X/XO. BASMC-embedded collagen gel lattice, which was pretreated with xanthine alone, showed contraction in response to ATP, thapsigargin, high-K+ solution, and A-23187. Pretreatment of the gel with X/XO impaired gel contraction not only by ATP and thapsigargin, but also by high-K+ solution and A-23187. The X/XO-treated gel showed normal contraction; however, when SOD was present during the pretreatment period. These results indicate that O[Formula: see text] attenuates smooth muscle contraction by impairing CRAC, ATP-induced Ca2+ transient, and Ca2+ sensitivity in BASMC.

scholarly journals Angiotensin II modulates calponin mRNA expression in aortic smooth muscle cells in vitro and in vivo

1998 ◽  
Vol 31 ◽  
pp. 104
Author(s):  
W.M.M. van de Greef ◽  
G. Castoldi ◽  
C.R.T. di Gioia ◽  
F. Clementi ◽  
M.-R. Camellitti ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Modulation of tissue factor expression by rapamycin and FK-506 in lipopolysaccharide-stimulated human mononuclear cells and serum-stimulated aortic smooth muscle cells

2005 ◽  
Vol 94 (07) ◽  
pp. 46-52 ◽  
Author(s):  
Soumeya Hammal ◽  
Nejma Ameziane ◽  
Marie-Thérèse Labro ◽  
Dominique de Prost ◽  
Véronique Ollivier
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
Tissue Factor ◽  
Mononuclear Cells ◽  
Muscle Cells ◽  
Monocytic Cells ◽  
Fk 506 ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

SummaryInflammation is a key pathogenic component of atherosclerosis; it also promotes thrombosis, a process underlying acute coronary events and stroke. Cells present in atherosclerotic plaque show abnormal tissue factor (TF) expression. Macrolides, in addition to their antimicrobial properties, have antiinflammatory effects that might help prevent atherothrombosis. The aim of this study was to determine the effect of an immunosuppressant macrolide, rapamycin (Sirolimus), on the expression of TF and its inhibitor (TFPI) by monocytic cells (human blood mononuclear and THP-1 cells) and human aortic smooth muscle cells, in comparison with FK-506 and azithromycin. In monocytic cells, rapamycin and FK-506 inhibited LPS-induced TF activity, antigen and mRNA expression through a transcriptional mechanism involving NF-κB. In smooth muscle cells, rapamycin and azithromycin had no effect on serum-induced TF expression, while FK-506 increased serum-induced TF protein and mRNA expression. TFPI levels in the culture supernatants of serum-stimulated smooth muscle cells were not modified by any of the three macrolides. Rapamycin slightly inhibits TFPI induction by LPS in monocytic cells. In addition to its recently established efficacy in the prevention of stent restenosis, the inhibitory effect of rapamycin on theTF pathway might have interesting therapeutic implications.

scholarly journals Dipyridamole Suppresses High Glucose-Induced Osteopontin Secretion and mRNA Expression in Rat Aortic Smooth Muscle Cells

2010 ◽  
Vol 74 (6) ◽  
pp. 1242-1250 ◽  
Author(s):  
Ming-Song Hsieh ◽  
Wen-Bin Zhong ◽  
Shu-Chuan Yu ◽  
John Yi-Chung Lin ◽  
Wei-Ming Chi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
High Glucose ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

scholarly journals Effect of high glucose concentrations on prostacyclin-stimulating factor mRNA expression in cultured aortic smooth muscle cells

Diabetologia ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 134-140 ◽  
Author(s):  
Y. Ono ◽  
F. Umeda ◽  
M. Kunisaki ◽  
N. Sekiguchi ◽  
T. Hashimoto ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
High Glucose ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Stimulating Factor
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