scholarly journals Monocyte Chemoattractant Protein 1–Mediated Migration of Mesenchymal Stem Cells Is a Source of Intimal Hyperplasia

2013 ◽  
Vol 33 (6) ◽  
pp. 1271-1279 ◽  
Author(s):  
Monika K. Grudzinska ◽  
Ewa Kurzejamska ◽  
Krzysztof Bojakowski ◽  
Joanna Soin ◽  
Michael H. Lehmann ◽  
...  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiangcheng Zhan ◽  
Jingwei Zhang ◽  
Saiyang Li ◽  
Xiaolu Zhang ◽  
Linchao Li ◽  
...  

Abstract Background Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell development in vitro. Results Intratesticular injection of MCP-1(10 ng/testis) into Leydig cell-depleted rat testis from post-EDS day 14 to 28 significantly increased serum testosterone and luteinizing hormone levels, up-regulated the expression of Leydig cell proteins, LHCGR, SCARB1, CYP11A1, HSD3B1, CYP17A1, and HSD17B3 without affecting progenitor Leydig cell proliferation, as well as increased ERK1/2 phosphorylation. MCP-1 (100 ng/ml) significantly increased medium testosterone levels and up-regulated LHCGR, CYP11A1, and HSD3B1 expression without affecting EdU incorporation into stem cells after in vitro culture for 7 days. RS102895, a CCR2 inhibitor, reversed MCP-1-mediated increase of testosterone level after culture in combination with MCP-1. Conclusion MCP-1 stimulates the differentiation of stem and progenitor Leydig cells without affecting their proliferation.


Stem Cells ◽  
2014 ◽  
Vol 32 (7) ◽  
pp. 1843-1854 ◽  
Author(s):  
Jia Liu ◽  
Weiqiang Li ◽  
Yinfen Wang ◽  
Wendong Fan ◽  
Panlong Li ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133746 ◽  
Author(s):  
Anna Labedz-Maslowska ◽  
Barbara Lipert ◽  
Dominika Berdecka ◽  
Sylwia Kedracka-Krok ◽  
Urszula Jankowska ◽  
...  

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