scholarly journals Inhibition of biofilm formation of periodontal pathogens by D-Arabinose

2021 ◽  
Vol 46 (3) ◽  
pp. 111-118
Author(s):  
Sun-Jin An ◽  
◽  
Jong-Uk Namkung ◽  
Kyung-Won Ha ◽  
Hye-Kyoung Jun ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Periodontal Pathogens

scholarly journals The Anti-biofilm Activity of Nanometric Zinc doped Bioactive Glass against Putative Periodontal Pathogens: An in vitro Study

2018 ◽  
Vol 4 (1) ◽  
pp. 95-107
Author(s):  
Nasrin Esfahanizadeh ◽  
Mohammad Reza Nourani ◽  
Abbas Bahador ◽  
Nasrin Akhondi ◽  
Mostafa Montazeri
Keyword(s):  
Biofilm Formation ◽  
Sol Gel ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Antibiofilm Activity ◽  
Periodontal Pathogens ◽  
Microplate Reader ◽  
45S5 Bioglass

Abstract Colonization of periodontal pathogens on the surgical sites is one of the primary reasons for the failure of regenerative periodontal therapies. Bioactive glasses (BGs) owing to their favorable structural and antimicrobial properties have been proposed as promising materials for the reconstruction of periodontal and peri-implant bone defects. This study aimed to investigate the antibiofilm activity of zinc-doped BG (Zn/BG) compared with 45S5 Bioglass® (BG®) on putative periodontal pathogens. In this in vitro experimental study, the nano BG doped with 5-mol% zinc and BG® were synthesized by sol-gel method. Mono-species biofilms of Aggregatibacter actinomycetemcomitans (A. a), Porphyromonas gingivalis (P. g), and Prevotella intermedia (P. i)were prepared separately in a well-containing microplate. After 48 hours of exposure to generated materials at 37°C, the anti-biofilm potential of the samples was studied by measuring the optical density (OD) at 570nm wavelengths with a microplate reader. Two-way ANOVA then analyzed the results. Both Zn/BG and BG® significantly reduced the biofilm formation ability of all examined strains after 48 hours of incubation (P=0.0001). Moreover, the anti-biofilm activity of Zn/BG was significantly stronger than BG® (P=0.0001), which resulted in the formation of a weak biofilm (OD<1) compared with a moderately adhered biofilm observed with BG® (1<OD<2). Zn/BG showed a significant inhibitory effect on the biofilm formation of all examined periodontal pathogens. Given the enhanced regenerative and anti-biofilm properties of this novel biomaterial, further investigations are required for its implementation in clinical situations.

scholarly journals Antibacterial Activity of Boswellia sacra Flueck. Oleoresin Extract against Porphyromonas gingivalis Periodontal Pathogen

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 859
Author(s):  
Nashwah G. M. Attallah ◽  
Walaa A. Negm ◽  
Engy Elekhnawy ◽  
Najla Altwaijry ◽  
Elshaymaa I. Elmongy ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Extracellular Polysaccharide ◽  
Surface Hydrophobicity ◽  
Periodontal Pathogen ◽  
Volatile Components ◽  
Antibiofilm Activity ◽  
Periodontal Pathogens ◽  
Boswellia Sacra

Boswellia sacra Flueck. oleoresin extract (frankincense) has traditionally been used in the treatment of different diseases, but there are no sufficient studies on its potential activity against periodontal pathogens. Therefore, antibacterial and antibiofilm activity of frankincense extract against Porphyromonas gingivalis clinical isolates were studied. The phytochemical composition of the volatile components of the extract was identified by GC-MS analysis revealing 49 compounds as trans-nerolidyl formate, cycloartenol acetate, ursenoic acid 3-oxomethyl ester, bisabolene epoxide, and kaur-16-ene. It decreased the growth and increased the leakage of nucleotides in 58.3% and 33.3% of isolates, respectively. Additionally, it reduced the extracellular polysaccharide production and the cell surface hydrophobicity in 41.67% and 50% of the isolates, respectively. Crystal violet assay revealed inhibition of biofilm formation by the tested isolates. Light microscope and scanning electron microscope were used to examine the biofilms and they confirmed the reduction of biofilm formation by frankincense extract. Downregulation of the genes linked to biofilm formation (fimA, hagA, and hagB) was observed using qRT-PCR after treatment with the frankincense extract. This study suggested that the frankincense extract could exhibit antibacterial and antibiofilm activity against P. gingivalis isolates. Thus, the frankincense extract could be used as a treatment approach for periodontitis.

scholarly journals Short-Chain N-Acylhomoserine Lactone Quorum-Sensing Molecules Promote Periodontal Pathogens in In Vitro Oral Biofilms

2019 ◽  
Vol 86 (3) ◽  
Author(s):  
Andrea Muras ◽  
Celia Mayer ◽  
Paz Otero-Casal ◽  
Rob A. M. Exterkate ◽  
Bernd W. Brandt ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Dental Plaque ◽  
Biofilm Formation ◽  
Lactic Acid Production ◽  
Plaque Formation ◽  
Microbial Colonization ◽  
Periodontal Pathogens ◽  
Gum Disease

ABSTRACT Acylhomoserine lactones (AHLs), the quorum-sensing (QS) signals produced by a range of Gram-negative bacteria, are involved in biofilm formation in many pathogenic and environmental bacteria. Nevertheless, the current paradigm excludes a role of AHLs in dental plaque formation, while other QS signals, such as AI-2 and autoinducer peptides, have been demonstrated to play an important role in biofilm formation and virulence-related gene expression in oral pathogens. In the present work, we have explored the effect of externally added AHLs on in vitro oral biofilm models for commensal, cariogenic, and periodontal dental plaque. While little effect on bacterial growth was observed, some AHLs specifically affected the lactic acid production and protease activity of the biofilms. Most importantly, the analysis of bacterial diversity in the biofilms showed that the addition of C6-homoserine lactone (C6-HSL) results in a shift toward a periodontal bacterial composition profile by increasing the relative presence of the orange-complex bacteria Peptostreptococcus and Prevotella. These results point to a relevant role of AHL-mediated QS in dental plaque formation and might be involved in the development of dysbiosis, the mechanism of which should be further investigated. This finding potentially opens new opportunities for the prevention or treatment of the periodontal disease. IMPORTANCE Dental plaque is omnipresent in healthy oral cavities and part of our commensal microbial colonization. At the same time, dental plaque is the cause of the most common human diseases, caries and gum disease. Dental plaque consists of billions of microbes attached to the surface of your teeth. Communication among these microbes is pivotal for development of these complex communities yet poorly studied in dental plaque. In the present study, we show that a specific communication molecule induces changes within the community related to the development of gum disease. This finding suggests that interfering with microbial communication may represent an interesting novel strategy to prevent gum disease that should be further investigated.

scholarly journals Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fariza Abrão ◽  
Thayná Souza Silva ◽  
Claudia L. Moura ◽  
Sérgio Ricardo Ambrósio ◽  
Rodrigo Cassio Sola Veneziani ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Biofilm Formation ◽  
Clinical Isolates ◽  
Antibiofilm Activity ◽  
Inhibition Assay ◽  
Periodontal Pathogens ◽  
Protease Inhibition ◽  
Periodontal Tissues ◽  
Causative Agents

AbstractInvasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evaluates the antibacterial and antivirulence activity of oleoresins obtained from different copaifera species and of ten isolated compounds against two causative agents of periodontitis. The following assays were performed: determination of the minimum inhibitory concentration (MIC), determination of the minimum bactericidal concentration (MBC), and determination of the antibiofilm activity by inhibition of biofilm formation and biofilm eradication tests. The antivirulence activity was assessed by hemagglutination, P. gingivalis Arg-X and Lis-X cysteine protease inhibition assay, and A. actinomycetemcomitans leukotoxin inhibition assay. The MIC and MBC of the oleoresins and isolated compounds 1, 2, and 3 ranged from 1.59 to 50 μg/mL against P. gingivalis (ATCC 33277) and clinical isolates and from 6.25 to 400 μg/mL against A. actinomycetemcomitans (ATCC 43717) and clinical isolates. About the antibiofilm activity, the oleoresins and isolated compounds 1, 2, and 3 inhibited biofilm formation by at least 50% and eradicated pre-formed P. gingivalis and A. actinomycetemcomitans biofilms in the monospecies and multispecies modes. A promising activity concerning cysteine protease and leucotoxin inhibition was also evident. In addition, molecular docking analysis was performed. The investigated oleoresins and their compounds may play an important role in the search for novel sources of agents that can act against periodontal pathogens.

scholarly journals Evaluation of the Antibacterial Effects and Mechanism of Plantaricin 149 from Lactobacillus Plantarum NRIC 149 on the Peri-Implantitis Pathogens

2021 ◽  
Author(s):  
Xiaolong Lin ◽  
Jiajia Xu ◽  
Yuedan Xu ◽  
Zhiwei Shi ◽  
Tao Fu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Lactobacillus Plantarum ◽  
Biofilm Formation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Periodontal Pathogens ◽  
Patch Clamp Technique ◽  
Marrow Stromal Cell ◽  
Channel Currents ◽  
Scanning Electron

Abstract Peri-implantitis is a common reversible disease after tooth implantation, caused by a variety of pathogenic microorganisms. Based on non-surgical or surgical treatment principles, supplementation by local or systemic drugs might enhance treatment efficacy. Porphyromonas gingivalis (Pg) (ATCC 33277) and Prevotella intermedius (Pi) (ATCC 25611) were used as test strains. The effects of Pln 149 on the biofilm formation and growth of four periodontal pathogens were evaluated by RT-PCR, fluorescence microscopy, and scanning electron microscopy. The antibacterial mechanism was tested by the patch-clamp technique. The cytotoxicity of Pln 149 (125 µg/ml) to bone marrow stromal cell (BMSC) was assessed using an MTT assay. Pln 149 exhibited significant inhibitory effects on Pg and Pi (P<0.05), with significant differences in the biofilm images of fluorescence microscope and scanning electron microscope (P<0.05). Pln 149 could change the sodium channel currents and exerted no cytotoxicity on bone marrow stromal cell. Pln 149 could inhibit the biofilm formation and growth of periodontal pathogens. Considering the absence of antimicrobial resistance and cytotoxicity, we suggest that the Pln 149 from Lactobacillus plantarum 149 might be a promising option for managing peri-implantitis.

Bacterial biofilm behavior in water-supply lines of dental units

1992 ◽  
Vol 50 (1) ◽  
pp. 882-883
Author(s):  
B.D. Tall ◽  
K.S. George ◽  
R. T. Gray ◽  
H.N. Williams
Keyword(s):  
Water Supply ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Bacterial Biofilm

Studies of bacterial behavior in many environments have shown that most organisms attach to surfaces, forming communities of microcolonies called biofilms. In contaminated medical devices, biofilms may serve both as reservoirs and as inocula for the initiation of infections. Recently, there has been much concern about the potential of dental units to transmit infections. Because the mechanisms of biofilm formation are ill-defined, we investigated the behavior and formation of a biofilm associated with tubing leading to the water syringe of a dental unit over a period of 1 month.

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