Die Vermehrung Von Globodera Rostochiensis (Wollenweber, 1923) Behrens, 1975, Pathotyp 1, in Wurzeln Resistenter Solanum Tuberosum Ssp. Andigena-Hybriden

Nematologica ◽  
1988 ◽  
Vol 34 (2) ◽  
pp. 182-187
Author(s):  
H. Stelter
Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Ken Devine ◽  
Peter Jones

AbstractIn studies using three sibling F1 clones from each of five crosses between Solanum tuberosum and different wild tuberbearing Solanum species, root leachate hatching activity towards each of the two potato cyst nematode (PCN) species, Globodera rostochiensis and G. pallida, segregated independently. Sephadex G-10 fractionation of the leachates of S. sucrense-hybrid clones revealed differences between clones in the hatching factor elution profiles for the two PCN species. Analysis of individual hatching factors indicated examples both of selectivity (both PCN species hatched in response to a particular hatching factor, but at different levels of hatching response) and of specificity (only one PCN species hatched in response to a particular hatching factor) of hatching factors. The hatching factor profiles of the S. sucrense-hybrids indicated segregation of hatching factors from the parents, but also detected novel factors unique to specific hybrid clones. Total hatching activity of the root leachate of the S. sucrense clones was positively correlated with the proportion of S. tuberosum-derived hatching factors.


2019 ◽  
Vol 22 (2) ◽  
pp. 10-32
Author(s):  
J. Gabriel, A. Terán, S. Veramendi, M. Baldelomar, G. Main

Las enfermedades causadas por virus y nematodos reducen los rendimientos y la calidad del tubérculo semilla en los cultivos de papa de todo el mundo, por lo que se toman diversas medidas y esfuerzos en investigación para combatir estas enfermedades. Esta investigación se planteó con el objetivo de validar tres marcadores moleculares asociados a genes de resistencia al nematodo quiste (Globodera rostochiensis y G. pallida) y al virus PVY en cultivares mejorados de papa. El estudio se realizó en condiciones de laboratorio e invernadero. En laboratorio la PCR permitió evaluar la presencia de los genes de resistencia a través del marcador RySC3 para resistencia al virus PVY y los marcadores HC y Gro 1-4 para resistencia a G. pallida y G. rostochiensis respectivamente. En invernadero el análisis de resistencia y/o susceptibilidad se determinó a través de la absorbancia (método DASELISA) y severidad para el virus PVY y la tasa de multiplicación en raíz (TMR) para el nematodo del quiste. Se verificó que el alelo para el marcador RySC3 está co-localizado con el gen Ryadg para resistencia a PVY, que fue observado en todos los cultivares a excepción de Huaycha. Por otro lado el alelo para el marcador HC que co-localiza con el gen/QTL RGp5-vrnHC para resistencia a G. pallida, se observó en 14 de las 20 cultivares evaluados, es decir el 70% mostraron el alelo de resistencia y el alelo para el marcador Gro1-4 que co-localiza con el gen Gro1-4 para resistencia a G. rostochiensis se observó en 7 de los 20 cultivares, es decir el 35% de los cultivares mostraron el alelo de resistencia a G. rostochiensis.


2021 ◽  
Vol 13 (4) ◽  
pp. 11083
Author(s):  
Sanaa A. HAROON ◽  
Zafar HANDOO ◽  
Mihail KANTOR ◽  
Andrea SKANTAR ◽  
Maria HULT

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich (1959) is a damaging soilborne quarantine pest of Solanum tuberosum (potato) and other solanaceous crops worldwide. In spring of 2021 a survey was conducted in area of Abo El Matamer, Bahera governorates in Egypt. Soil samples were taken in zigzag pattern throughout 65 acres of potato cultivated land and processed in Nematology lab, Fayoum University, Egypt. In June 2021, two hundred soil samples were collected from nearby areas to evaluate the distribution of this potato cyst nematode in other cultivated land located in area of first infection but fortunately the golden potato cyst nematode was not detected from neighboring locations. The nematode species was identified by both morphological and molecular means as Globodera rostochiensis. To our knowledge this is the first molecular and morphological characterization of G. rostochiensis from Egypt.


Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 293 ◽  
Author(s):  
I. Niragire ◽  
M. Couvreur ◽  
G. Karssen ◽  
B. Uwumukiza ◽  
W. Bert

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 3082
Author(s):  
L. Cortada ◽  
J. Omagwa ◽  
J. Kisitu ◽  
M. Adhiambo ◽  
S. Haukeland ◽  
...  

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Omaira Pineda ◽  
Merideth W. Bonierbale ◽  
Robert L. Plaisted ◽  
Bill B. Brodie ◽  
Steven D. Tanksley

The H1 gene from Solanum tuberosum ssp. andigena confers high levels of resistance to the potato cyst nematode Globodera rostochiensis and is used extensively in potato breeding. Using a dihaploid segregating population, a search was conducted for linkage between this gene and markers on the potato/tomato RFLP map. A total of 60 RFLP markers covering the entire genome were screened on bulk resistant and susceptible segregants. Linkage was indicated for eight markers on chromosome 5. Individual plant analysis placed the closest marker, CD78, at a maximum map distance of 2.7 cM from H1. A molecular marker for the H1 should be useful both as a correlative screening tool for incorporation of resistance into new cultivars and as starting point for map-based cloning of this important gene.Key words: Solanum tuberosum, potato, resistance genes, Globodera rostochiensis, H1, RFLP, cyst nematodes.


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