Allelic state of the molecular marker for golden nematode (Globodera rostochiensis) resistance gene H1 among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars

2013 ◽  
Vol 47 (5) ◽  
pp. 294-297 ◽  
Author(s):  
A. V. Karelov ◽  
L. A. Pylypenko ◽  
N. O. Kozub ◽  
R. O. Bondus ◽  
O. I. Borzykh ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Marker ◽  
Resistance Gene ◽  
Globodera Rostochiensis ◽  
Golden Nematode ◽  
Allelic State

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont.) de Bary

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 249-257 ◽  
Author(s):  
A. El-Kharbotly ◽  
J. M. E. Jacobs ◽  
B. te Lintel Hekkert ◽  
W. J. Stiekema ◽  
A. Pereira ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Transgenic Potato ◽  
Potato Genome ◽  
Rflp Map ◽  
Polymerase Chain ◽  
R1 Gene ◽  
Map Location

The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reaction or plasmid rescue techniques and used as probes for RFLP linkage analysis. The RFLP map location of 60 T-DNAs carrying Ds–NPTII was determined. The T-DNA distribution per chromosome and the relative distance between them appeared to be random. All 12 chromosomes have been covered with Ds-containing T-DNAs, potentially enabling tagging of any gene in the potato genome. The T-DNA insertions of two transformants, BET92-Ds-A16-259 and BET92-Ds-A16-416, were linked in repulsion to the position of the resistance gene R1 against Phytophthora infestans. After crossing BET92-Ds-A16-416 with a susceptible parent, 4 desired recombinants (Ds carrying T-DNA linked in coupling phase with the R1 gene) were discovered. These will be used for tagging the R1 gene. The efficiency of the pathway from the introduction to localization of T-DNAs is discussed. Key words : Solanum tuberosum, Phytophthora infestans, Ds element, transposon tagging, R genes, euchromatin.

Development of a molecular marker for a bruchid (Callosobruchus chinensis L.) resistance gene in mungbean

Euphytica ◽  
2007 ◽  
Vol 157 (1-2) ◽  
pp. 113-122 ◽  
Author(s):  
Huei-Mei Chen ◽  
Chien-An Liu ◽  
C. George Kuo ◽  
Ching-Mei Chien ◽  
Horng-Chi Sun ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Resistance Gene ◽  
Callosobruchus Chinensis

Potato cyst nematode hatching activity and hatching factors in inter-specific Solanum hybrids

Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Ken Devine ◽  
Peter Jones
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Species Analysis ◽  
Specific Hybrid ◽  
Hybrid Clones ◽  
Different Levels

AbstractIn studies using three sibling F1 clones from each of five crosses between Solanum tuberosum and different wild tuberbearing Solanum species, root leachate hatching activity towards each of the two potato cyst nematode (PCN) species, Globodera rostochiensis and G. pallida, segregated independently. Sephadex G-10 fractionation of the leachates of S. sucrense-hybrid clones revealed differences between clones in the hatching factor elution profiles for the two PCN species. Analysis of individual hatching factors indicated examples both of selectivity (both PCN species hatched in response to a particular hatching factor, but at different levels of hatching response) and of specificity (only one PCN species hatched in response to a particular hatching factor) of hatching factors. The hatching factor profiles of the S. sucrense-hybrids indicated segregation of hatching factors from the parents, but also detected novel factors unique to specific hybrid clones. Total hatching activity of the root leachate of the S. sucrense clones was positively correlated with the proportion of S. tuberosum-derived hatching factors.

scholarly journals Marcadores moleculares asociados a genes de resistencia para Globodera sp. y virus PVY en papa (Solanum tuberosum L.)

2019 ◽  
Vol 22 (2) ◽  
pp. 10-32
Author(s):  
J. Gabriel, A. Terán, S. Veramendi, M. Baldelomar, G. Main
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Globodera Rostochiensis

Las enfermedades causadas por virus y nematodos reducen los rendimientos y la calidad del tubérculo semilla en los cultivos de papa de todo el mundo, por lo que se toman diversas medidas y esfuerzos en investigación para combatir estas enfermedades. Esta investigación se planteó con el objetivo de validar tres marcadores moleculares asociados a genes de resistencia al nematodo quiste (Globodera rostochiensis y G. pallida) y al virus PVY en cultivares mejorados de papa. El estudio se realizó en condiciones de laboratorio e invernadero. En laboratorio la PCR permitió evaluar la presencia de los genes de resistencia a través del marcador RySC3 para resistencia al virus PVY y los marcadores HC y Gro 1-4 para resistencia a G. pallida y G. rostochiensis respectivamente. En invernadero el análisis de resistencia y/o susceptibilidad se determinó a través de la absorbancia (método DASELISA) y severidad para el virus PVY y la tasa de multiplicación en raíz (TMR) para el nematodo del quiste. Se verificó que el alelo para el marcador RySC3 está co-localizado con el gen Ryadg para resistencia a PVY, que fue observado en todos los cultivares a excepción de Huaycha. Por otro lado el alelo para el marcador HC que co-localiza con el gen/QTL RGp5-vrnHC para resistencia a G. pallida, se observó en 14 de las 20 cultivares evaluados, es decir el 70% mostraron el alelo de resistencia y el alelo para el marcador Gro1-4 que co-localiza con el gen Gro1-4 para resistencia a G. rostochiensis se observó en 7 de los 20 cultivares, es decir el 35% de los cultivares mostraron el alelo de resistencia a G. rostochiensis.

scholarly journals Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt

2021 ◽  
Vol 13 (4) ◽  
pp. 11083
Author(s):  
Sanaa A. HAROON ◽  
Zafar HANDOO ◽  
Mihail KANTOR ◽  
Andrea SKANTAR ◽  
Maria HULT
Keyword(s):  
Solanum Tuberosum ◽  
Nematode Species ◽  
Morphological Characterization ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Cultivated Land ◽  
Zigzag Pattern

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich (1959) is a damaging soilborne quarantine pest of Solanum tuberosum (potato) and other solanaceous crops worldwide. In spring of 2021 a survey was conducted in area of Abo El Matamer, Bahera governorates in Egypt. Soil samples were taken in zigzag pattern throughout 65 acres of potato cultivated land and processed in Nematology lab, Fayoum University, Egypt. In June 2021, two hundred soil samples were collected from nearby areas to evaluate the distribution of this potato cyst nematode in other cultivated land located in area of first infection but fortunately the golden potato cyst nematode was not detected from neighboring locations. The nematode species was identified by both morphological and molecular means as Globodera rostochiensis. To our knowledge this is the first molecular and morphological characterization of G. rostochiensis from Egypt.

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