scholarly journals Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt

2021 ◽  
Vol 13 (4) ◽  
pp. 11083
Author(s):  
Sanaa A. HAROON ◽  
Zafar HANDOO ◽  
Mihail KANTOR ◽  
Andrea SKANTAR ◽  
Maria HULT
Keyword(s):  
Solanum Tuberosum ◽  
Nematode Species ◽  
Morphological Characterization ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Cultivated Land ◽  
Zigzag Pattern

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich (1959) is a damaging soilborne quarantine pest of Solanum tuberosum (potato) and other solanaceous crops worldwide. In spring of 2021 a survey was conducted in area of Abo El Matamer, Bahera governorates in Egypt. Soil samples were taken in zigzag pattern throughout 65 acres of potato cultivated land and processed in Nematology lab, Fayoum University, Egypt. In June 2021, two hundred soil samples were collected from nearby areas to evaluate the distribution of this potato cyst nematode in other cultivated land located in area of first infection but fortunately the golden potato cyst nematode was not detected from neighboring locations. The nematode species was identified by both morphological and molecular means as Globodera rostochiensis. To our knowledge this is the first molecular and morphological characterization of G. rostochiensis from Egypt.

Potato cyst nematode hatching activity and hatching factors in inter-specific Solanum hybrids

Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Ken Devine ◽  
Peter Jones
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Species Analysis ◽  
Specific Hybrid ◽  
Hybrid Clones ◽  
Different Levels

AbstractIn studies using three sibling F1 clones from each of five crosses between Solanum tuberosum and different wild tuberbearing Solanum species, root leachate hatching activity towards each of the two potato cyst nematode (PCN) species, Globodera rostochiensis and G. pallida, segregated independently. Sephadex G-10 fractionation of the leachates of S. sucrense-hybrid clones revealed differences between clones in the hatching factor elution profiles for the two PCN species. Analysis of individual hatching factors indicated examples both of selectivity (both PCN species hatched in response to a particular hatching factor, but at different levels of hatching response) and of specificity (only one PCN species hatched in response to a particular hatching factor) of hatching factors. The hatching factor profiles of the S. sucrense-hybrids indicated segregation of hatching factors from the parents, but also detected novel factors unique to specific hybrid clones. Total hatching activity of the root leachate of the S. sucrense clones was positively correlated with the proportion of S. tuberosum-derived hatching factors.

Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations

Nematology ◽  
1999 ◽  
Vol 1 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Asier Fullaondo ◽  
Enrique Barrena ◽  
Miguel Viribay ◽  
Imanol Barrena ◽  
Azucena Salazar ◽  
...  
Keyword(s):  
Nematode Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Detection Methods ◽  
South American ◽  
Specific Primer ◽  
Pomme De Terre ◽  
Highly Sensitive ◽  
Specific Band

AbstractRAPD fragments specific for potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida were identified. These were used to derive two primer combinations for PCR which amplify only one specific band in each species. The primer combinations were evaluated on 39 samples of PCN populations containing all currently described pathotypes as well as some South American PCN populations. This method for species identification is highly sensitive and also allows the detection of species mixtures in a sample. Furthermore, the generation of unique amplification products facilitates the application of automatic detection methods without electrophoretic separation and is therefore particularly useful for routine diagnoses. Identification des especes de nematodes a kyste de la pomme de terre Globodera rostochiensis et G. pallida par PCR grace a l'utilisation de combinaisons d'amorces specifiques - Des fragments RAPD specifiques des especes de nematodes a kyste de la pomme de terre (PCN) Globodera rostochiensis et G. pallida, ont ete identifies. A partir de ces fragments, deux combinaisons d'amorces, capables d'amplifier en PCR une seule bande, specifique pour chaque espece, ont ete obtenus. Les combinaisons d'amorces ont ete evaluees sur 39 echantillons de populations de PCN contenant tous les pathotypes decrits ainsi que quelques populations provenant d'Amerique du Sud. Cette methode d'identification d'espece est tres sensible et elle permet de plus l'identification d'un melange d'especes dans un meme echantillon. En sus, la generation de produits uniques d'amplification permet l'utilisation de methodes d'identification automatisees, sans separation electrophoretique, tres utiles donc pour le diagnostic de routine.

scholarly journals Globodera rostochiensis (Woll.) Behrens (Tylenchida, Heteroderidae), the only potato cyst nematode species found in Finland

1992 ◽  
Vol 1 (5) ◽  
pp. 519-525
Author(s):  
Jari Heikkilä ◽  
Kari Tiilikkala
Keyword(s):  
Isoelectric Focusing ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Potato Seed ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Production Area ◽  
Dimensional Electrophoresis

About 10000 soil samples, 519 thereof infected with potato cyst nematode (PCN), were studied during 1984-1988. Cysts from infected samples were tested by isoelectric focusing to identify PCN species. All the infected samples were also tested with H1-resistant (Satuma) and susceptible (Bintje) potato cultivars to separate resistance breaking populations. Cysts from the roots of Satuma were tested by two-dimensional electrophoresis. The potato seed production area in Finland was found to be free of PCN of any kind. In other parts of Finland all tested samples revealed G. rostochiensis banding pattern, but no G. pallida was found. Except for the most common pathotype Ro1-Ro4, we only found Ro2.

Characterisation of potato cyst nematode populations from Portugal

Nematology ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Maria José Da Cunha ◽  
Isabel Luci Da Conceição ◽  
Isabel Maria De O. Abrantes ◽  
Ken Evans ◽  
M. Susana De A. Santos
Keyword(s):  
Isoelectric Focusing ◽  
Specific Protein ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Direct Sampling ◽  
Species Specific ◽  
The University

Abstract Populations of potato cyst nematode (PCN) were assembled from all of the potato growing regions of Portugal, either by direct sampling of fields or from cyst samples sent to the University of Coimbra for identification. When 30 or more cysts were available, isoelectric focusing of species-specific protein bands was used to identify which PCN species were present. PCN was present in 35% of a set of 226 soil samples. PCN was also identified in a larger set of 423 populations. Of the 423 populations, 83% were pure Globodera rostochiensis, 8% were pure G. pallida and 9% were mixtures of the two. PCN was found in all of the main potato growing areas but G. pallida was not detected in Coimbra, Setúbal or Viana do Castelo districts.

scholarly journals First Report of the Golden Potato Cyst Nematode, Globodera rostochiensis, in Bosnia and Herzegovina

Plant Disease ◽  
2011 ◽  
Vol 95 (7) ◽  
pp. 883-883
Author(s):  
I. Ostojić ◽  
D. Grubišić ◽  
M. Zovko ◽  
T. Miličević ◽  
T. Gotlin Čuljak
Keyword(s):  
Soil Sample ◽  
Bosnia And Herzegovina ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Ware Potato ◽  
Quarantine Species

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975, is the most problematic pest of potato (Solanum tuberosum L. subsp. tuberosum) worldwide and it is recognized as an A1 plant health quarantine species. In Bosnia and Herzegovina, there have been no surveys for the presence of potato cyst nematodes (PCN) in ware potato lands, although they have been documented in the nearby countries of Serbia and Croatia (3). To determine the presence and distribution of PCN and to prevent further spread or increase of PCN populations, a survey in ware potato land was undertaken in 2008, 2009, and 2010. Soil samples consisting of 100 cores of 4 to 5 ml of soil were taken in a grid pattern throughout the plots. Samples of approximately 500 g were processed in the laboratory. The samples were air dried and cysts were extracted using a Spears's flotation method (4) from a 100-ml subsample. Species identification was based on morphometrical characters (2) and real-time PCR where the ribosomal internal transcribed spacer (ITS5, PITSr3) region was amplified and detected by the Sequence Dection Software v1.3 (Applied Biosystems, Carlsbad, CA) (1). In 2008, soil sampling was conducted in five locations and PCN were not detected in any of 10 soil samples taken. In June 2009, 17 soil samples were collected from seven locations. Two viable cysts of G. rostochiensis were detected in one soil sample from Tihaljina (Zaside; E 6447659.560, N 4797392.101, altitude 130 m). A total of 110 soil samples were collected from 90 locations in May and June 2010. Five viable cysts of G. rostochiensis were detected in one soil sample taken from Čapljina (Opličići – Turajlovina; E 6485169, N 4775964, altitude 222 m). On the basis of the survey results, G. rostochiensis is not widely spread but the cysts are detectable, indicating that the infestation is relatively recent and that there is a threat of further spread of this quarantine species. Consequently, producers need to grow resistant potato cultivars and implement additional control measures to keep the nematode populations below damaging levels. References: (1) J. Bacic et al. Russ. J. Nematol. 16:1, 61, 2008. (2) C. C. Fleming and T. O. Powers. Potato Cyst Nematodes: Biology, Distribution and Control. R. J. Marks and B. B. Brodie, eds.CAB International, Wallingford, 1998. (3) D. Grubišic et al. J. Pest Sci. 80:21, 2007. (4) J. F. Spears. Agr. Handb. No. 353. U.S. Dep. Agric., 1968.

scholarly journals First Report of Potato Cyst Nematode (Globodera rostochiensis) Infecting Potato (Solanum tuberosum) in Rwanda

Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 293 ◽  
Author(s):  
I. Niragire ◽  
M. Couvreur ◽  
G. Karssen ◽  
B. Uwumukiza ◽  
W. Bert
Keyword(s):  
Solanum Tuberosum ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
First Report

scholarly journals First Report of Potato Cyst Nematode, Globodera rostochiensis, Infecting Potato (Solanum tuberosum) in Uganda

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 3082
Author(s):  
L. Cortada ◽  
J. Omagwa ◽  
J. Kisitu ◽  
M. Adhiambo ◽  
S. Haukeland ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
First Report

Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematode Globodera rostochiensis

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Omaira Pineda ◽  
Merideth W. Bonierbale ◽  
Robert L. Plaisted ◽  
Bill B. Brodie ◽  
Steven D. Tanksley
Keyword(s):  
Solanum Tuberosum ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Rflp Markers ◽  
Individual Plant ◽  
Cyst Nematodes ◽  
Entire Genome ◽  
Rflp Map ◽  
Starting Point

The H1 gene from Solanum tuberosum ssp. andigena confers high levels of resistance to the potato cyst nematode Globodera rostochiensis and is used extensively in potato breeding. Using a dihaploid segregating population, a search was conducted for linkage between this gene and markers on the potato/tomato RFLP map. A total of 60 RFLP markers covering the entire genome were screened on bulk resistant and susceptible segregants. Linkage was indicated for eight markers on chromosome 5. Individual plant analysis placed the closest marker, CD78, at a maximum map distance of 2.7 cM from H1. A molecular marker for the H1 should be useful both as a correlative screening tool for incorporation of resistance into new cultivars and as starting point for map-based cloning of this important gene.Key words: Solanum tuberosum, potato, resistance genes, Globodera rostochiensis, H1, RFLP, cyst nematodes.

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