Description of Ruehmaphelenchus taedae n. sp. (Nematoda: Aphelenchoididae) found in Loblolly pine logs from the USA

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Xiaoling Lü ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Loblolly Pine ◽  
Excretory Pore ◽  
Lateral Field ◽  
Female Tail ◽  
The Status ◽  
The Usa ◽  
Males And Females ◽  
A New Species

Summary Ruehmaphelenchus taedae n. sp., isolated from Loblolly pine logs (Pinus taedae L.) from the USA, is described and figured. It is characterised by a relatively slim body (a = 42 and 43 for males and females, respectively), three lines in the lateral field, male spicules relatively small (12-18 μm) with high and dorsally bent condylus and weakly developed rostrum, bursal flap absent, short tail possessing a long terminal spike ending in a bluntly rounded tip and 8.7-13.3 μm long, vulva positioned at ca 83% of body length, vulval flap absent, vulval lips slightly protruding, post-vulval uterine branch extending for less than half of vulva to anus distance, and female tail conoid, ca 3-4 anal body diam. long, with 13.7-18.5 μm terminal projection. The new species can be separated from all other species of the genus by the male tail possessing a long terminal spike and the more anterior excretory pore. Detailed phylogenetic analysis based on 28S D2-D3 region sequences confirmed the status of this nematode as a new species.

Bursaphelenchus paraleoni n. sp. (Tylenchina: Aphelenchoididae) intercepted in Loblolly pine logs imported into China from the USA

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Xinxin Ma ◽  
Xiaoling Lu
Keyword(s):  
New Species ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Precloacal Papilla ◽  
Long Tail ◽  
Lateral Field ◽  
The Usa ◽  
Males And Females ◽  
A New Species ◽  
Huge Variation

Summary Bursaphelenchus paraleoni n. sp. was isolated from Loblolly pine, Pinus taeda, logs from the USA. The new species is characterised by a lateral field with three lines, 13-16 μm long stylet with small basal swellings, and the excretory pore located slightly posterior to the nerve ring. The male spicules are mitten-shaped, the lamina smoothly tapering towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular or conical with finely rounded tip, and cucullus absent. One midventral precloacal papilla, one adcloacal pair and two postcloacal pairs of genital papillae are present. The bursal flap is long and spade-like with the posterior margin truncate or irregular. Females have a small vulval flap 4-6 μm long, a post-vulval uterine sac extending for 34-49% of the vulva to anus distance, a very long tail with slight ventral curvature, mostly filiform and with a finely rounded terminus, occasionally bluntly rounded. Huge variation in the tails of males and females were observed in a culture. It is similar to species of B. eidmanni, B. leoni, and B. silvestris in the leoni-group but detailed phylogenetic analysis based on 18S, ITS and D2-D3 28S sequences confirmed it as a new species.

Bursaphelenchus parayongensis n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from China

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Ute Schönfeld ◽  
Xinxin Ma ◽  
Xiaoling Lü
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Posterior Part ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
The Status ◽  
A New Species ◽  
Partial 18S

Summary Bursaphelenchus parayongensis n. sp. was isolated from packaging wood of a consignment from China exported to Germany. The new species is characterised by the lateral field with three lines, 9-13 μm long stylet with small basal swellings, and the excretory pore located near the posterior part of metacorpus. The male spicules are mitten-shaped, the lamina smoothly tapers towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular with sharply pointed tip, and without cucullus. One precloacal papilla, one adcloacal pair and two postcloacal pairs of male papillae present, with the posteriormost pair forming ‘gland papillae’. Bursal flap long, spade-like with posterior margin truncate or irregular. Females have a small vulval flap of 2-3 μm long, and a post-vulval uterine sac extending for 55-77% of the vulva to anus distance. Tail cylindrical, mostly (about 70%) with a robust mucron, very short to less than 3 μm, but occasionally tail terminus bluntly rounded, without mucron. It is similar to species in subgroup 3 of the eggersi group including B. carpini, B. clavicauda, B. cryphali and B. yongensis, but morphologically different. Detailed phylogenetic analysis based on partial 18S, ITS and D2-D3 region of 28S sequences has confirmed the status of this nematode as a new species.

Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaph-elenchidae) in packaging wood from Singapore—a new species of the B. xylophilus group

Zootaxa ◽  
2005 ◽  
Vol 988 (1) ◽  
pp. 1 ◽  
Author(s):  
JIANFENG GU ◽  
JIANCHENG ZHANG ◽  
HELEN BRAASCH ◽  
WOLFGANG BURGERMEISTER
Keyword(s):  
New Species ◽  
Body Length ◽  
Morphological Differentiation ◽  
Deciduous Trees ◽  
Lateral Field ◽  
Distal Extremity ◽  
Female Tail ◽  
Males And Females ◽  
Group B ◽  
A New Species

Bursaphelenchus singaporensis sp. n. isolated in China from packaging wood of deciduous trees, imported from Singapore is described and illustrated. This new species clearly belongs to the B. xylophilus group, having males with the typically shaped spicules with a cucullus at their distal extremity, the typical position and number of caudal papillae (three pairs and one single) and the anterior vulval lip of the females developed as a distinct flap. The new species is characterized by a body length of 792 (553–950) µm and 850 (690–961) µm of males and females, respectively, robust body (a= 34 and 31, resp.), 15–16 µm-long stylet, lateral field with four lines, long postuterine branch (averaging 102 µm) and a strongly conoid female tail (c= 20) with a finely rounded, only slightly ventrally-bent terminus, male with very strong spicules (41–48 µm long), distinct rostrum and small cucullus, and a dorso-ventral visible terminal bursa. Bursaphelenchus singaporensis sp. n. is closely related to other species of the B. xylophilus group (B. xylophilus, B. mucronatus, B. kolymensis, B. fraudulentus, B. conicaudatus, B. baujardi and B. luxuriosae) and similar to B. abruptus. The morphological differentiation is mainly based on the shape of the female tail. However, B. singaporensis sp. n. differs from all other species of the B. xylophilus group by larger spicules. The new species can be differentiated from B. abruptus, B. xylophilus, B. mucronatus, B. fraudulentus, B. conicaudatus and B. luxuriosae by means of ITS-RFLP patterns.

Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hongkong, China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 557-566 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Weijun Duan ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Female Ratio ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Abstract Bursaphelenchus paraparvispicularis n. sp. is described and figured from pine packaging wood originating in Hongkong, China, and inspected in Ningbo harbour, China. The new species clearly belongs to the hofmanni group. It is characterised by a relatively stout body (a = 26.7 and 26.5 for males and females, respectively), three lines in the lateral field, seven caudal papillae, spicules relatively small (12.6-15.3 μm), mitten-shaped, with lamina dorsal line smoothly arcuate but calomus relatively straight, condylus squared or round, well developed, rostrum well developed with round terminus, cucullus absent, the shape of the female tail, which is short and ventrally bent with a bluntly pointed terminus, and vulval lips not forming a vulval flap. The new species is morphologically closest to B. parvispicularis and can be distinguished by smaller and stouter body, lower female ratio c′ (average 2.8 vs 4.4) and longer spicule condylus. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 and partial LSU sequences, which revealed that B. paraparvispicularis n. sp. is closest to B. parvispicularis.

Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Botrytis Cinerea ◽  
Bursaphelenchus Xylophilus ◽  
Lateral Field ◽  
Female Tail ◽  
Phylogenetic Status ◽  
Rflp Profile ◽  
Molecular Phylogenetic ◽  
Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 709-723 ◽  
Author(s):  
Zhen Wang ◽  
Wim Bert ◽  
Jianfeng Gu ◽  
Marjolein Couvreur ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Medicago Sativa ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male Tail ◽  
The Usa ◽  
Group 3 ◽  
A New Species

Summary Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μm long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μm long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis.

Description of Stegelletina lingulata sp. n. (Nematoda, Rhabditida, Cephalobidae) from xeric environments in Iran

Zootaxa ◽  
2017 ◽  
Vol 4358 (3) ◽  
pp. 462
Author(s):  
JOAQUÍN ABOLAFIA ◽  
EBRAHIM SHOKOOHI
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Small Body ◽  
Reproductive Systems ◽  
Lateral Field ◽  
Male Tail ◽  
Line Drawings ◽  
Female Tail ◽  
Body Diameter ◽  
A New Species

A new species belonging to the genus Stegelletina, S. lingulata sp. n., is described from xeric natural areas from Iran. It is characterized by having small body (0.51–0.69 mm in females and 0.45–0.64 mm in males), cuticle slightly tessellated especially at anterior end, lateral field with three longitudinal incisures, lips with long acute process at primary axils and three more dentate processes at secondary axils, primary axils with one triangular axillar guard process and secondary axils lacking axillar processes, labial probolae long (9–11 µm) with slender peduncle lacking adaxial expansion and with convergent smooth prongs, stoma, pharynx and reproductive systems cephaloboid, excretory pore at isthmus level, spermatheca 1.1–1.4 times, and post-vulval sac 0.9–1.2 times the corresponding body diameters in length, female rectum 0.6–0.8 times anal body diameter long, female tail conoid (35–47 µm long, c = 14.0–15.6, c' = 2.1–2.7) with fine rounded terminus, male tail conoid, curved ventrad (53–59 µm long, c = 11.0–12.0, c' = 1.9–2.5) with rounded terminus, spicules 31–33 µm long with ventrally straight manubrium and gubernaculum 14–17 µm long. Measurements and illustrations (line drawings, LM and SEM) are provided for this species. In addition, comments on the morphology of the lip region of the species of the genus Stegelletina are included. 

Description of Ruehmaphelenchus quercophilus n. sp. (Nematoda: Aphelenchoididae) from dying oak, Quercus robur, in Poland

Nematology ◽  
2017 ◽  
Vol 19 (9) ◽  
pp. 1051-1064
Author(s):  
Munawar Maria ◽  
Jianfeng Gu ◽  
Marek Tomalak ◽  
Yiwu Fang ◽  
Hongmei Li
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Quercus Robur ◽  
Nerve Ring ◽  
Its Sequences ◽  
Lateral Lines ◽  
The Status ◽  
A New Species

Ruehmaphelenchus quercophilus n. sp., isolated from a dying oak, Quercus robur, in the State Forests, Łopuchówko District, Poland, is described and figured. It is characterised by three lateral lines, relatively stout body, excretory pore posterior to nerve ring, five caudal papillae, spicules relatively small (12-14 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 78% of body length, vulval flap absent, post-uterine branch extending for slightly over half of vulva to anus distance, and conoid tail with a bend at the distal end forming a terminal projection 10-14 μm long. The new species is very similar to four other described species, namely R. formosanus, R. juliae, R. thailandae, and R. sirisus. Detailed phylogenetic analysis based on 18S, 28S D2-D3 and ITS sequences has confirmed the status of this nematode as a new species.

Description of Bursaphelenchus irokophilus n. sp. (Nematoda: Aphelenchoididae) isolated from Milicia excelsa (Welw.) C.C. Berg wood imported into Italy from Cameroon

Nematology ◽  
2019 ◽  
Vol 21 (9) ◽  
pp. 957-969 ◽  
Author(s):  
Giulia Torrini ◽  
Agostino Strangi ◽  
Giuseppe Mazza ◽  
Leonardo Marianelli ◽  
Pio Federico Roversi ◽  
...  
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Central Italy ◽  
Lateral Field ◽  
Female Tail ◽  
Milicia Excelsa ◽  
The Relationship ◽  
A New Species ◽  
Molecular Characters

Summary A new species of Bursaphelenchus, extracted from unprocessed logs of Milicia excelsa from Cameroon and arriving in the port of Leghorn, Tuscany, Central Italy, is described. Bursaphelenchus irokophilus n. sp. is based upon morphological and molecular characters and is characterised by the two-lined lateral field, mitten-shaped spicule possessing cucullus and well-developed and slightly dorsally curved condylus with narrowly rounded tip, female vulva with side flap, and long, smoothly tapering and strongly ventrally recurved female tail. The new species belongs to the abietinus-group and is similar to, but distinguished from, B. abietinus and B. rainulfi by the position of secretory-excretory pore, longer and slender female tail and some morphometric values. In addition, the phylogeny based on SSU and D2-D3 LSU regions shows that this species belongs to the abietinus-group, the relationship among the species of this group being discussed.

Sign in / Sign up

Export Citation Format

Share Document