Scanning electron microscopic studies on spermatozoa of anurans from India and Sri Lanka

2001 ◽  
Vol 22 (3) ◽  
pp. 303-308 ◽  
Author(s):  
Hareesh Joshy ◽  
Mitsuru Kuramoto

AbstractThe shape and size of spermatozoa of 11 frog species from India and Sri Lanka were examined by scanning electron microscopy. The spermatozoa of the genera Limnonectes and Euphlyctis were of the generalized type with a thick sperm head and a thin tail, whereas Indirana semipalmata had peculiar spermatozoa with a densely coiled sperm head and a thick tail. Rhacophorus microtympanum is likely to belong to the genus Philautus from sperm morphology. The spermatozoa of Microhyla ornata and Ramanella obscura were very similar, with a cone-shaped sperm head and a thin tail.

1989 ◽  
Vol 51 (4) ◽  
pp. 775-791 ◽  
Author(s):  
Mitsuo ABE ◽  
Kazushige TAKEHANA ◽  
Kenji IWASA ◽  
Takeo HIRAGA

1979 ◽  
Vol 88 (6_suppl4) ◽  
pp. 2-14 ◽  
Author(s):  
Malcolm D. Graham ◽  
Rodney Perkins

The structure of the normal human stapes was studied with the scanning electron microscope. Specimens were obtained 48 hours after death from adult human temporal bones free from obvious inflammatory disease. The specimens were fixed, dissected, critical-point dried and coated with gold. In this scanning electron microscopic study an attempt has been made to systematically demonstrate the average scanning electron microscopic features of various areas of the normal human stapes. An emphasis has been placed upon demonstrating as clearly as possible the details previously unclear or unrecognized and duplication of many excellent earlier light and electron microscopic studies has not been attempted. The typical appearance of the stapes head, neck, arch, crura and footplate has been presented. It is apparent that there exists a high degree of structural specialization particularly in the stapes arch and footplate area.


2017 ◽  
Vol 9 (1) ◽  
pp. 461-465
Author(s):  
Tanvi Sharma ◽  
Neelam Joshi ◽  
Anu Kalia

This work was aimed to identify the LC50 of the indigenous fungal isolates for controlling L. erysimi infesta-tion in mustard aphid besides to probe the mechanism of action of the local isolates and comparison of the efficacy with the reference culture and commercial formulation ‘Mycojaal’. Three isolates of entomopathogenic fungi Beauveria bassiana were tested for infection on nymph of Lipaphis erysimi Kalt. using scanning electron microscopy (SEM) to record any variation. The SEM revealed adhesion of spores of B.bassiana followed by penetration of L.erysimi nymph surface. It was observed that all Beauveria isolates showed little variation with respect to penetration and adhesion at different time intervals. Further, lethal concentration (LC50) values of B. bassiana isolates against L. erysimi was recorded and was lowest (0.05x107 spores/ml) in B. bassiana MTCC 4495 and highest (0.11.X107 spores/ml) was recorded in native isolate F10 after 120 hours of treatment. The study has established the need for the isolation and evaluation of the indigenous Beauveria isolate. Moreover, it also exhibited the efficacy of the reference and commercially available biocontrol agents.


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