Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Crustaceana ◽  
2015 ◽  
Vol 88 (1) ◽  
pp. 51-65 ◽  
Author(s):  
María S. Michiels ◽  
Juana C. del Valle ◽  
Alejandra A. López Mañanes
Keyword(s):  
Cyclic Amp ◽  
Direct Effect ◽  
Lipase Activity ◽  
Ecological Research ◽  
Biochemical Characteristics ◽  
Neohelice Granulata ◽  
Concomitant Decrease ◽  
Heat Tolerant ◽  
Burrowing Crab

We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crabNeohelice granulata(Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent ), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10−4 M dopamine (about 100%), 2 × 10−3 M glucagon (about 250%) and 10−4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism.

The Influence of Competency of Preschool Teachers in Fujian Province on Subjective Well-Being: The Mediating Role of Psychological Capital

2019 ◽  
Author(s):  
Xuguang Sun ◽  
Ailing Huang
Keyword(s):  
Direct Effect ◽  
Psychological Capital ◽  
Preschool Teachers ◽  
Well Being ◽  
Mediating Effect ◽  
Subjective Well Being ◽  
Positive Psychological Capital ◽  
Mediating Role ◽  
Mediator Variable

The intermediary effect interval of the preschool teachers' competence characteristics → positive psychological capital → the subjective well-being of the preschool teachers is (0.23—0.55), does not contain 0, and the effect amount is 0.35. The competency characteristics of preschool teachers → The direct effect interval of subjective well-being of preschool teachers is (0.05—0.36), excluding 0, and the effect quantity is 0.20, indicating that positive psychological capital as a mediator variable has the characteristics of preschool teachers and the subjective well-being of preschool teachers. Partial mediating effect, the ratio of mediating effect to total effect is 64.01%.

Service Quality and Repeat Purchase: The Moderating Effect of Perceived Value, and Satisfaction

2020 ◽  
Vol 11 (1) ◽  
pp. 5-5
Author(s):  
Aprihatiningrum Hidayati ◽  
Agus W. Soehadi ◽  
Aji Hermawan ◽  
Hartoyo Hartoyo
Keyword(s):  
Service Quality ◽  
Direct Effect ◽  
Purchase Intention ◽  
Perceived Value ◽  
Moderating Variables ◽  
Mediating Role ◽  
Prior Literature ◽  
Repeat Purchase ◽  
Purchase Satisfaction

The purpose of this study is to examine the direct and indirect effect of these determinants on repeat purchase. Competitive training industries impose pressure on managers to increase repeat purchase. Even though service quality and repeat purchase are well studied in prior literature, their determinants such as perceived value and satisfaction have not been fully investigated together as moderating variables. Most of literature stated both of perceived value and satisfaction as independent variables. Besides, most of literature consider purchase as an attitudinal (namely purchase intention), while this study focus on behavioural (namely repeat purchase). Data from a survey of 300 customers of public training are used to empirically evaluate the model. Results indicate that perceived value and the satisfaction have no direct effect on repeat purchase. However, these constructs indirectly influence repeat purchase through the mediating role of service quality. Satisfaction and perceived value play a major role in enhancing service quality, but do not directly impact repeat purchase. Interestingly, the direct effect on repeat purchases stems from service quality while recent researches suggest satisfaction is the antecedent. The findings suggest that training companies should invest more resources aimed at enhancing service quality through service delivery training for their employees. Even though prior research has considered the concepts studied here, this study aims to empirically evaluate a variety of antecedent factors that potentially affect repeat purchase. Relationships are established utilizing data collected in Indonesia (an increasingly important consumer market) which adds value to extant knowledge in this area. Keywords: path analysis, perceived value, repeat purchase, satisfaction, service quali

scholarly journals Role of cyclic AMP in the control of cell-specific gene expression

1993 ◽  
Vol 4 (6) ◽  
pp. 204-209 ◽  
Author(s):  
Wolfgang Schmid ◽  
Doris Nitsch ◽  
Michael Boshart ◽  
Günther Schütz
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals Regulation of the expression of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene. Its role in the control of ketogenesis

1992 ◽  
Vol 283 (1) ◽  
pp. 261-264 ◽  
Author(s):  
N Casals ◽  
N Roca ◽  
M Guerrero ◽  
G Gil-Gómez ◽  
J Ayté ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Rat Liver ◽  
Genetic Expression ◽  
Fat Feeding

We have explored the role of mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase in regulating ketogenesis. We had previously cloned the cDNA for mitochondrial HMG-CoA synthase and have now studied the regulation in vivo of the expression of this gene in rat liver. The amount of processed mitochondrial HMG-CoA synthase mRNA is rapidly changed in response to cyclic AMP, insulin, dexamethasone and refeeding, and is greatly increased by starvation, fat feeding and diabetes. We conclude that one point of ketogenic control is exercised at the level of genetic expression of mitochondrial HMG-CoA synthase.

scholarly journals Heterologous desensitization of the cyclic AMP-independent glycogenolytic response in rat liver cells

1981 ◽  
Vol 200 (3) ◽  
pp. 509-514 ◽  
Author(s):  
B Bréant ◽  
S Keppens ◽  
H De Wulf
Keyword(s):  
Cyclic Amp ◽  
Glycogen Phosphorylase ◽  
Liver Glycogen ◽  
Receptor Interaction ◽  
Alpha Adrenergic Receptors ◽  
Rat Liver Cells ◽  
Heterologous Desensitization ◽  
Highly Correlated ◽  
Alpha Agonist

Vasopressin and alpha-adrenergic agonists are known to be potent cyclic AMP-independent Ca2+-dependent activators of liver glycogen phosphorylase. When hepatocytes are pre-incubated with increasing concentrations of vasopressin or of the alpha-agonist phenylephrine, they become progressively unresponsive to a second addition of the respective agonist. The relative abilities of six vasopressin analogues and of five alpha-agonists to activate glycogen phosphorylase and to cause subsequent desensitization are highly correlated, indicating that the same vasopressin and alpha-adrenergic receptors are involved in both responses. About 5-times-higher peptide concentrations are needed to desensitize the cells than to activate their glycogen phosphorylase, whereas the concentrations of alpha-agonists required for the desensitization are only twice those needed for the activation of phosphorylase. The desensitization is not mediated by a perturbation in the agonist-receptor interaction. It is clearly heterologous, i.e. it is not agonist-specific, and must therefore involve a mechanism common to both series of agonists. The evidence for a role of Ca2+ movements or phosphatidylinositol turnover is briefly discussed.

The role of cyclic AMP in canine secretin-stimulated bile flow

1979 ◽  
Vol 27 (1) ◽  
pp. 57-61 ◽  
Author(s):  
D.L. Kaminski ◽  
M.J. Ruwart ◽  
Y.G. Deshpande
Keyword(s):  
Cyclic Amp ◽  
Bile Flow
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