Molecular and phylogenetic studies on Pratylenchidae from Iran with additional data on Pratylenchus delattrei, Pratylenchoides alkani and two unknown species of Hirschmanniella and Pratylenchus

Nematology ◽  
2013 ◽  
Vol 15 (6) ◽  
pp. 633-651 ◽  
Author(s):  
Zahra Majd Taheri ◽  
Zahra Majd Taheri ◽  
Zahra Tanha Maafi ◽  
Zahra Majd Taheri ◽  
Zahra Tanha Maafi ◽  
...  
Keyword(s):  
Ornamental Plants ◽  
Rrna Gene ◽  
28S Rrna ◽  
Diagnostic Pcr ◽  
28S Rrna Gene ◽  
Phylogenetic Studies ◽  
Unknown Species ◽  
Tea Plantations ◽  
Cereal Fields ◽  
High Level

Thirteen species of Pratylenchidae: Pratylenchus coffeae, P. delattrei, P. loosi, P. neglectus, P. penetrans, P. pseudopratensis, P. thornei, P. vulnus, Pratylenchus sp., Pratylenchoides alkani, P. ritteri, Hirschmanniella sp. and Zygotylenchus guevarai were collected from different crops and plants throughout Iran. The specimens were identified using morphological and molecular methods. Morphometrics and morphology are given for Pratylenchus sp., P. delattrei, Pratylenchoides alkani and Hirschmanniella sp. The D2-D3 expansion segments of the 28S rRNA gene were amplified and sequenced for all 13 species studied. Diagnostic PCR-ITS-RFLP profiles are given for Pratylenchus delattrei, P. penetrans, P. pseudopratensis, Pratylenchus sp., Pratylenchoides alkani and P. ritteri. Pratylenchus neglectus and P. thornei, collected from cereal fields, P. loosi from tea plantations, P. coffeae from banana, P. penetrans from ornamental plants, P. vulnus from pines and Z. guevarai from almonds showed a high level of similarity in the D2-D3 sequences with corresponding GenBank sequences. Nucleotide differences between Iranian populations and reference species were in the intraspecific range. Pratylenchus delattrei, found in vegetable fields, and Pratylenchus sp. from palm rhizosphere, formed a highly supported clade with P. zeae, the two former species being morphologically very close to the latter except in tail shape. Pratylenchus pseudopratensis, from cereal fields, clustered with P. vulnus with low support. Phylogenetic relationships within Pratylenchus species were mainly congruent with those obtained in previous studies. Despite the morphological similarities between P. ritteri and P. alkani, the D2-D3 of 28S rRNA gene sequences differed by 5 bp. Hirschmanniella sp., from a rice field, formed a clade with H. loofi and H. kwazuna.

scholarly journals Differentiation of Basidiobolus spp. Isolates: RFLP of a Diagnostic PCR Amplicon Matches Sequence-Based Classification and Growth Temperature Preferences

2021 ◽  
Vol 7 (2) ◽  
pp. 110
Author(s):  
Maike Claussen ◽  
Stefan Schmidt
Keyword(s):  
Growth Temperature ◽  
Restriction Analysis ◽  
Screening Method ◽  
Rflp Analysis ◽  
Physiological Characteristics ◽  
Molecular Characteristics ◽  
Rrna Gene ◽  
28S Rrna ◽  
Diagnostic Pcr ◽  
28S Rrna Gene

The genus Basidiobolus, known since 1886, is primarily associated with reptiles and amphibians. Although globally distributed, rare infections caused by members of this genus mainly occur in tropical and subtropical regions. Morphological and physiological characteristics were used in the past for the description of species. However, some of these characteristics vary depending on culture conditions. Therefore, most species names are regarded as synonyms of B. ranarum as the only pathogenic species. Yet, not all environmental isolates are necessarily pathogenic. This study aimed to analyze if environmental Basidiobolus isolates can be distinguished reliably based on morpho-physiological and molecular characteristics. Eleven isolates originally obtained from feces of south African reptiles and one type strain, Basidiobolus microsporus DSM 3120, were examined morpho-physiologically. Sequence analysis of the 18S and partial 28S rRNA gene and restriction analysis of a diagnostic amplicon (restriction fragment length polymorphism, RFLP) were performed for all 12 strains. Based on the results obtained, morphological features and the 18S rRNA sequence proved insufficient for the reliable differentiation of isolates. However, isolates were distinguishable by growth temperature profiles, which matched isolate clusters established by partial 28S rRNA gene sequence and restriction analysis of a Basidiobolus specific diagnostic PCR amplicon. Our results indicate that RFLP analysis can be used as a fast screening method to identify Basidiobolus isolates with similar physiological characteristics.

scholarly journals Intra-species sequence variability in 28s rRNA gene of Oesophagostomum venulosum isolated from goats of West Bengal, India

2010 ◽  
Vol 3 (7) ◽  
pp. 515-518 ◽  
Author(s):  
Subhasish Bandyopadhyay ◽  
Asit Kumar Bera ◽  
Sourav Sikdar ◽  
Sumanta De ◽  
Subhashree Ghosh ◽  
...  
Keyword(s):  
West Bengal ◽  
Rrna Gene ◽  
28S Rrna ◽  
Sequence Variability ◽  
28S Rrna Gene

scholarly journals Phylogenetic Analysis of 28S rRNA Gene of Indigenous Xiphinema pachydermum

2019 ◽  
Vol 8 (07) ◽  
pp. 1960-1968
Author(s):  
Anju Sharma ◽  
Satish K. Sharma ◽  
Kiran Rana ◽  
Anil Kumar Verma
Keyword(s):  
Phylogenetic Analysis ◽  
Rrna Gene ◽  
28S Rrna ◽  
28S Rrna Gene

scholarly journals Molecular and morphological characterisation of Xiphinema granatum n. sp. and Longidorus pisi Edward, Misra & Singh, 1964 (Dorylaimida: Longidoridae) from Iran

Nematology ◽  
2012 ◽  
Vol 14 (8) ◽  
pp. 949-960 ◽  
Author(s):  
Majid Pedram ◽  
Ebrahim Pourjam ◽  
Juan E. Palomares-Rius ◽  
Razieh Ghaemi ◽  
Carolina Cantalapiedra-Navarrete ◽  
...  
Keyword(s):  
New Species ◽  
Species Group ◽  
Rrna Gene ◽  
28S Rrna ◽  
Morphometric Data ◽  
Molecular Analyses ◽  
Morphological Characterisation ◽  
28S Rrna Gene ◽  
Close Relationship ◽  
Female Genital

Xiphinema granatum n. sp. from pomegranate in Saveh, Markazi province, central Iran, is described and illustrated. The new species belongs to species group 8 of the genus Xiphinema sensu Loof & Luc, 1990. The new species is characterised by a body 3.5-4.1 mm long, 37-49 μm diam., odontostyle and odontophore 118-132 and 65-74 μm long, respectively, presence of sperm in the female genital tracts, uterus devoid of any Z-differentiation or spines, and abundant males with spicules 45-58 μm long. The polytomous identification codes of the new species are: A4, B4, C5a, D5, E56, F4, G23, H2, I3, J4, K2, L2. Beside morphological and morphometric data, molecular analyses of D2-D3 fragments of 28S rRNA gene placed the new species in close relationship with X. vuittenezi and X. italiae and some species belonging to Xiphinema morphospecies group 6, but clearly separated from X. index. In addition, an Iranian population of Longidorus pisi from the same locality is morphologically and molecularly characterised.

Placement of Cacoceria and phylogenetic relationships of the xylotine genera of the tribe Milesiini (Diptera, Syrphidae: Eristalinae) based on molecular characters

Zootaxa ◽  
2006 ◽  
Vol 1171 (1) ◽  
pp. 17 ◽  
Author(s):  
GUNILLA STÅHLS
Keyword(s):  
Intraspecific Variation ◽  
Phylogenetic Relationships ◽  
Rrna Gene ◽  
28S Rrna ◽  
Large Fragment ◽  
Mitochondrial Coi ◽  
Phylogenetic Placement ◽  
28S Rrna Gene ◽  
Molecular Characters

The phylogenetic relationships of traditional xylotine genera of tribe Milesiini (Diptera, Syrphidae: Eristalinae) were explored using molecular character of a large fragment of the mitochondrial COI and the D2-3 region of the nuclear ribosomal 28S rRNA gene. Of particular interest was the phylogenetic placement of the enigmatic Neotropical genus Cacoceria. The ingroup included 48 taxa, and Cheilosia illustrata (Rhingiini, Eristalinae) was used as outgroup. Multiple specimens of more common taxa were sequenced for surveying levels of intraspecific variation. The combined dataset was analysed using parsimony and optimisation alignment, using the program POY. Cacoceria was resolved within the Chalcosyrphus clade. Earlier hypotheses placed the taxon in the tribe Myoleptini or Chrysogasterini, or in Xylotini without stating a closer relationship to any particular xylotine taxon. The representatives of traditional xylotine taxa were resolved as ((Hadromyia + (Brachypalpoides + Blera + Lejota) + (Xylota sg. Hovaxylota + Xylota sg. Sterphoides) + (Xylota (including Sterphus)) while Neplas and Brachypalpus were resolved in the Myoleptini albeit with very low support.

scholarly journals Reconstruction of the phylogeny of the Opisthobranchia (Mollusca: Gastropoda) by means of 18s and 28s rRNA gene sequences

2005 ◽  
Vol 71 (2) ◽  
pp. 113-125 ◽  
Author(s):  
Verena Vonnemann ◽  
Michael Schrödl ◽  
Annette Klussmann-Kolb ◽  
Heike Wägele
Keyword(s):  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
28S Rrna Gene

Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H. koreana comb. n. and H. orientalis comb. n. (Tylenchida: Heteroderidae)

Nematology ◽  
2008 ◽  
Vol 10 (5) ◽  
pp. 611-632 ◽  
Author(s):  
Manuel Mundo-Ocampo ◽  
Alberto Troccoli ◽  
Sergei A. Subbotin ◽  
Julio Del Cid ◽  
James G. Baldwin ◽  
...  
Keyword(s):  
Morphological Identification ◽  
Rrna Gene ◽  
Similar Species ◽  
28S Rrna ◽  
Diagnostic Pcr ◽  
Second Stage ◽  
Pcr Rflp ◽  
Identification Of Species ◽  
Its Rrna ◽  
Labial Disc

Abstract Phylogenetic analysis of five gene fragments: ITS-rRNA, D2 and D3 of 28S rRNA, 18S rRNA, Hsp90 and actin, of Heterodera species and two representative Afenestrata species, A. koreana and A. orientalis, form a clade with H. cynodontis, H. bifenestra and an unidentified Heterodera sp. infecting grasses. Based on these results and the consideration that the key diagnostic characters of Afenestrata are convergent and do not define a clade, synonymisation of Afenestrata with Heterodera is proposed. The following new combinations are made: H. africana comb. n., H. axonopi comb. n., H. koreana comb. n., and H. orientalis comb. n. Furthermore, H. (= Afenestrata) sacchari is renamed as H. saccharophila nom. nov. to avoid homonymy. All these species, together with H. bamboosi, are regarded as members of a paraphyletic ‘Afenestrata group’ within Heterodera. Whilst recognised as artificial, the Afenestrata group is nevertheless an aid to discussion about these similar species. Morphological and molecular characterisation of populations of H. koreana comb. n. from Florida and H. orientalis comb. n. from Florida and Guatemala verify the identification of these populations as valid representatives for molecular studies of the species. Light and SEM observations also provide new detail and a broader understanding of the morphological range of both species. These include a longer stylet for females of H. koreana comb. n. and H. orientalis comb. n. than reported in the original descriptions. In addition, previously unreported tuberculate ridges are noted on the surface of vulval lips of H. orientalis comb. n. The lip region of second-stage juveniles of H. koreana comb. n. and H. orientalis comb. n. both include fused adjacent submedian lips that also fuse with the labial disc and the second lip annulus. The ITS-rRNA gene sequences of H. orientalis comb. n. populations from Florida and Guatemala were similar to those from the Russian type locality. Diagnostic PCR-RFLP of ITS-rRNA profiles with six enzymes for H. orientalis comb. n. and H. koreana comb. n. are given. A key for the morphological identification of species of the Afenestrata group is provided.

Phylogenetic position of the genus Mesochytrium (Chytridiomycota) based on zoospore ultrastructure and sequences from the 18S and 28S rRNA gene

Nova Hedwigia ◽  
2010 ◽  
Vol 90 (1) ◽  
pp. 81-94 ◽  
Author(s):  
Sergey A. Karpov ◽  
Peter M. Letcher ◽  
Maria A. Mamkaeva ◽  
Kira A. Mamkaeva
Keyword(s):  
Phylogenetic Position ◽  
Rrna Gene ◽  
28S Rrna ◽  
28S Rrna Gene
Sign in / Sign up

Export Citation Format

Share Document