Utilisation of entomopathogenic nematodes, Heterorhabditis spp. and Steinernema spp., for the control of Agrotis ipsilon (Lepidoptera, Noctuidae) in China

Nematology ◽  
2014 ◽  
Vol 16 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Xun Yan ◽  
Xiandong Wang ◽  
Richou Han ◽  
Xuehong Qiu
Keyword(s):  
Larval Stage ◽  
Entomopathogenic Nematodes ◽  
Field Trials ◽  
Fourth Instar ◽  
Agrotis Ipsilon ◽  
Exposure Rate ◽  
Black Cutworm ◽  
The Third ◽  
Lepidoptera Noctuidae

Entomopathogenic nematodes (EPN) have good application prospects for the control of the black cutworm, Agrotis ipsilon. In the present study, the effects of isolate, exposure rate, temperature and larval stage on EPN infectivity to A. ipsilon were evaluated. Results of in vitro tests showed that Steinernema carpocapsae Mex and Heterorhabditis indica LN2 were the most virulent and promising species, causing 80.0 and 83.3% mortality, respectively, to the third instar larvae at 25°C and 72 h after infection. Mortality of A. ipsilon caused by the nematodes was significantly affected by EPN exposure rate, temperature and the larval stage. Both S. carpocapsae and H. indica caused greater mortality to the third instar of A. ipsilon at 25°C than at 15, 20 and 30°C. Both EPN isolates also caused higher mortality to the second instar than to the third and fourth instar larvae of A. ipsilon. The field trials of EPN for the control of A. ipsilon also showed that S. carpocapsae Mex and H. indica LN2 reduced the damage caused by A. ipsilon and increased the cabbage yield when compared with the control. Both EPN isolates showed better control effects than cyfluthrin and Bacillus thuringiensis, indicating that these two EPN isolates could be used for sustainable control of A. ipsilon in vegetable fields in China.

scholarly journals Canadian entomopathogenic nematode isolates: virulence against black cutworm (Lepidoptera: Noctuidae)

2013 ◽  
Vol 93 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Guy Bélair ◽  
Louis Simard ◽  
Julie Dionne
Keyword(s):  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Steinernema Carpocapsae ◽  
Agrotis Ipsilon ◽  
Black Cutworm ◽  
Laboratory Conditions ◽  
Lepidoptera Noctuidae

The virulence of ten indigenous and two commercial isolates of entomopathogenic nematodes against the black cutworm, Agrotis ipsilon, was assessed under laboratory conditions. When comparing commercial isolates, Steinernema carpocapsae exhibited higher virulence than S. feltiae. One indigenous isolate of S. carpocapsae (6Sc) provided similar or higher virulence than the commercial isolate against black cutworm larvae. An indigenous strain of S. kraussei demonstrated low virulence in our study.

Field Evaluation of Steinernema carpocapsae (Rhabditida: Steinernematidae) Against Black Cutworm (Lepidoptera: Noctuidae) Larvae In Field Corn

1992 ◽  
Vol 27 (4) ◽  
pp. 427-435 ◽  
Author(s):  
Eli Levine ◽  
Hassan Oloumi-Sadeghi
Keyword(s):  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Field Evaluation ◽  
Growing Season ◽  
Steinernema Carpocapsae ◽  
Agrotis Ipsilon ◽  
Black Cutworm ◽  
Field Corn ◽  
Lepidoptera Noctuidae ◽  
Better Than

An entomopathogenic nematode, Steinernema carpocapsae (Weiser) All strain (BioVector®), applied at two rates (1.25 and 2.5 × 109 nematodes/ha) was compared with several registered insecticides for controlling black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), larvae during the 1991 growing season in Illinois. Fonofos, tefluthrin and chlorpyrifos were applied at planting time; permethrin and the two rates of nematodes were applied as postemergence rescue treatments. The nematodes generally performed as well as or better than the conventional insecticides in controlling black cutworm larval injury to seedling corn. Bioassays with black cutworm larvae showed that nematode efficacy was lost 8 days after application in the field. Entomopathogenic nematodes hold promise for controlling black cutworms in corn, particularly for corn grown under irrigation.

Efficacy of Entomopathogenic Nematodes Against the Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

2019 ◽  
Author(s):  
Xun Yan ◽  
Muhammad Shahid Arain ◽  
Yinying Lin ◽  
Xinghui Gu ◽  
Limeng Zhang ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Entomopathogenic Nematodes ◽  
Larval Mortality ◽  
Mortality Rates ◽  
The Other ◽  
Exposure Rate ◽  
The Third ◽  
Tobacco Cutworm ◽  
Different Temperatures ◽  
Lepidoptera Noctuidae

Abstract Spodoptera litura (F.) is an obnoxious cosmopolitan pest that causes serious damage to different economic crops. Entomopathogenic nematodes (EPN) have the potential to control the S. litura larvae. Fifteen EPN isolates were screened, and Steinernema sp. 64-2, four isolates of S. carpocapsae (Weiser), S. longicaudum (Shen & Wang) X-7, and two isolates of H. indica (Poinar, Karunaka & David) were found to cause higher mortality of the second, third, and fourth instars of S. litura than the other tested isolates, with larval mortality rates > 90% after 48 h of exposure. An exposure rate of 12.5 infective juveniles per larva was enough for S. carpocapsae A24, All, and G-R3a-2 and S. longicaudum X-7 to cause 100% mortality of the second instar, and for S. longicaudum X-7 and H. indica 212-2 to cause 100% mortality of the third instar. Five EPN isolates were tested on their virulence at different temperatures and found that all the five EPN isolates performed well against the S. litura larvae at 25 and 30°C, but were not active at 10 and 15°C. Two S. carpocapsae isolates (All and Mex) were virulent against the S. litura larvae at lower temperatures. The five tested EPN isolates were also found to have the ability to infect and kill the pupae of S. litura in the laboratory. The present study further proves that EPN are effective at controlling S. litura, which may partially substitute the use of chemical insecticides, thus reduce the overuse of chemical insecticides.

scholarly journals The Novel Agrotis ipsilon Nora Virus Confers Deleterious Effects to the Fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae)

2021 ◽  
Vol 12 ◽  
Author(s):  
Tong Li ◽  
Ruobing Guan ◽  
Yuqing Wu ◽  
Su Chen ◽  
Guohui Yuan ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Agrotis Ipsilon ◽  
The Novel ◽  
Black Cutworm ◽  
A Genome ◽  
Nora Virus ◽  
Novel Host ◽  
Lepidoptera Noctuidae

In the present study, we identified a novel, positive-sense single-stranded RNA virus in the Chinese black cutworm, Agrotis ipsilon. It has a genome length of 11,312 nucleotides, excluding the poly(A) tails, and contains five open reading frames. The ORF2 encodes the conserved domains of RNA helicase and RNA-dependent RNA polymerase, while ORF4 and 5 encode three viral proteins. Herein, the A. ipsilon virus was clustered with a Helicoverpa armigera Nora virus and was thus provisionally named “Agrotis ipsilon Nora virus” (AINV). AINV was successfully transmitted into a novel host, Spodoptera frugiperda, through injection, causing a stable infection. This found the possibility of horizontal AINV transmission among moths belonging to the same taxonomic family. Nonetheless, AINV infection was deleterious to S. frugiperda and mainly mediated by antiviral and amino acid metabolism-related pathways. Furthermore, the infection significantly increased the S. frugiperda larval period but significantly reduced its moth eclosion rate. It suggests that AINV is probably to be a parasitic virus of S. frugiperda.

Sign in / Sign up

Export Citation Format

Share Document