Steinernema beitlechemi n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from South Africa

Nematology ◽  
2016 ◽  
Vol 18 (4) ◽  
pp. 439-453 ◽  
Author(s):  
Harun Çimen ◽  
Vladimír Půža ◽  
Jiří Nermuť ◽  
Justin Hatting ◽  
Tshima Ramakuwela ◽  
...  
Keyword(s):  
South Africa ◽  
New Species ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Monophyletic Clade ◽  
African Species ◽  
Spicule Length ◽  
Tail Tip ◽  
A New Species

Steinernema beitlechemi n. sp. is a new species of entomopathogenic nematode isolated from soil samples collected in Bethlehem, Free State province, South Africa. The infective juveniles of S. beitlechemi n. sp. are characterised by a body length of 696 (606-768) μm, position of nerve ring of 96 (86-105) μm from the head and lateral fields with six ridges (i.e., seven lines) in mid-body. First-generation males of S. beitlechemi n. sp. can be distinguished by a spicule length of 93 (88-100) μm and 12 pairs of genital papillae. First-generation females have a moderately protruding vulva and a rounded tail tip bearing one spine-like projection. The new species is further characterised by sequences of the ITS and partial 28S regions of the ribosomal DNA. Phylogenetic analyses show S. beitlechemi n. sp. as a member of a strongly supported monophyletic clade with three other African species: S. sacchari, S. cameroonense and S. nyetense.

Steinernema pwaniensis n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Tanzania

2016 ◽  
Vol 91 (1) ◽  
pp. 20-34 ◽  
Author(s):  
V. Půža ◽  
J. Nermut ◽  
Z. Mráček ◽  
S. Gengler ◽  
S. Haukeland
Keyword(s):  
New Species ◽  
First Generation ◽  
Second Generation ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
East African ◽  
African Species ◽  
Group Members ◽  
Tail Tip

AbstractA new species of entomopathogenic nematode, Steinernema pwaniensis n. sp., belonging to the glaseri group, is described from Tanzania. The infective juveniles of S. pwaniensis n. sp. are characterized by a body length of 978 μm (808–1131), distance from anterior end to excretory pore of 86 μm (80–95) and a tail length of 87 μm (75–95). The ratios a, c and E% of S. pwaniensis n. sp. are substantially lower than those of all other African ‘glaseri’ group members. The first-generation males of S. pwaniensis n. sp. can be distinguished by a large spicule length of 92 μm (80–97) and by the absence of the caudal mucron, while second-generation males possess a short spine-like mucron. First-generation females have a peg-like tail tip bearing three spine-like projections. Second-generation females can be recognized by a slightly protruding vulva and well-developed post-anal swelling. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions of the ribosomal DNA. Phylogenetic analyses show that S. pwaniensis n. sp. forms a strongly supported monophyletic clade with two other East African species, S. ethiopiense and S. karii.

Steinernema nguyeni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa

Nematology ◽  
2016 ◽  
Vol 18 (5) ◽  
pp. 571-590 ◽  
Author(s):  
Antoinette P. Malan ◽  
Rinus Knoetze ◽  
Louwrens R. Tiedt
Keyword(s):  
South Africa ◽  
New Species ◽  
Ribosomal Dna ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
Infective Juvenile ◽  
Lateral Field ◽  
Spicule Length ◽  
Molecular Features ◽  
Generation Female

Steinernema nguyenin. sp. was recovered by baiting from beneath anOlea africanatree in South Africa. The combination of morphological and molecular features suggests thatS. nguyenin. sp. is a member of thefeltiae-kraussei-oregonensegroup, clustering with members of this group in Clade III. The new species is morphologically characterised by the infective juvenile body length of 737 (673-796) μm and the number of ridges in the infective juvenile lateral field is 2, 8, 2. The male of the first generation can be recognised by the spicule length of 66 (58-75) μm and a gubernaculum length of 43 (30-55) μm. The first generation female can be recognised by the vulval lips only slightly protruding and the presence of low, double-flapped epiptygmata. Analysis of the ITS and D2-D3 regions of the ribosomal DNA confirms thatS. nguyenin. sp. differs from all other knownSteinernemaspecies.

Steinernema phyllophagae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Florida, USA

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 425-442 ◽  
Author(s):  
Eileen Buss ◽  
Khuong Nguyen
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
White Grub ◽  
Lateral Field ◽  
Quercus Virginiana ◽  
Live Oak ◽  
Tail Tip ◽  
A New Species

AbstractA new species of entomopathogenic nematode, Steinernema phyllophagae n. sp., was isolated from a white grub (Phyllophaga sp.) in a live oak (Quercus virginiana) nursery. The new species is characterised by morphometrics of the infective Sjuvenile (IJ) with body length 1289 (1133-1395) μm, distance from anterior end to the excretory pore 99 (84-120) μm, tail 89 (77-109) μm, ratio a = 31 (27.6-35.5), H% = 57 (51-60) and E% = 110 (70-139). The pattern of the lateral field of IJ of the new species is 2, 5, 6, 8, 6, 2. The male of the first generation can be recognised by the length and shape of the spicule and gubernaculum, position of the excretory pore, D% = 75 (68-80), and GS% = 71 (61-77). The female can be recognised by the vulva having low epiptygma and by the presence of several papilla-like structures near the tail tip. Steinernema phyllophagae n. sp. differs further from other species of Steinernema by characteristics of ITS and D2D3 regions of rDNA.

Steinernema kandii n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from northern Benin

Nematology ◽  
2019 ◽  
Vol 21 (2) ◽  
pp. 107-128 ◽  
Author(s):  
Anique Godjo ◽  
Leonard Afouda ◽  
Hugues Baimey ◽  
Marjolein Couvreur ◽  
Lionel Zadji ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
28S Rdna ◽  
Infective Juvenile ◽  
Type Isolate ◽  
Spicule Length ◽  
A New Species

Summary Two nematode isolates from the genus Steinernema were collected in northern Benin. Morphological, morphometric, molecular and cross-hybridisation studies placed these nematodes into a new species, Steinernema kandii n. sp., within the bicornutum-group. Phylogenetic analyses based on both ITS and D2-D3 regions of 28S rDNA revealed that S. kandii n. sp. is different from all known Steinernema species and sister to S. abbasi (97.3-97.6% ITS nucleotide similarity) and S. bifurcatum (98.3-98.4% D2-D3 similarity). Steinernema kandii n. sp. can be separated from other members of the bicornutum-group by the greater infective juvenile (IJ) max. body diam. of 35 (27-48) μm (type isolate). It differs from S. abbasi by the greater IJ body length 707 (632-833) μm (type isolate), EP distance 55 (52-60) μm (type isolate), spicule length 67 (57-75) μm (type isolate) and the occurrence of one pair of genital papillae at the cloacal aperture.

Steinernema lamjungense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Lamjung district, Nepal

Nematology ◽  
2011 ◽  
Vol 13 (5) ◽  
pp. 589-605 ◽  
Author(s):  
Hari Bahadur Khatri-Chhetri ◽  
Lieven Waeyenberge ◽  
Sergei Spiridonov ◽  
Hira Kaji Manandhar ◽  
Maurice Moens
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
First Generation ◽  
Second Generation ◽  
Entomopathogenic Nematode ◽  
Soil Samples ◽  
Field Pattern ◽  
Lateral Field ◽  
Spicule Length ◽  
A New Species

Abstract A new species of entomopathogenic nematode, Steinernema lamjungense n. sp., was recovered from soil samples collected from Tarku, Lamjung district of Nepal, during a survey in 2007. The analysis of ITS-rDNA sequence placed S. lamjungense n. sp. in the arenarium-glaseri-karii-longicaudum group. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of different stages and by characterisations and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by medium-long body length of 832 (690-950) μm, pharynx length of 127 (110-142) μm, H% = 61 (55-69) and E% = 79 (69-97). The lateral field pattern is 2, 6, 7, 8, 3, 2. The males have well curved, yellowish-brown spicules with a prominent velum. The spicule terminus is blunt with a longitudinal ventral notch. Spicule length is 87 (81-94) μm and gubernaculum length is 57 (50-66) μm in the first generation males. A tail mucron is absent in first but present in second generation males. First generation males have a lateral field with a single ridge. A post-anal swelling is not developed in first generation females but is well developed in second generation females. Three other isolates of the same species were recovered from the neighbourhood of the type locality and yet another isolate was collected from Swarketari, Syangja district.

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

scholarly journals Heterorhabditis noenieputensis n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa

2012 ◽  
Vol 88 (2) ◽  
pp. 139-151 ◽  
Author(s):  
A.P. Malan ◽  
R. Knoetze ◽  
L. Tiedt
Keyword(s):  
South Africa ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Morphological Traits ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
The Body ◽  
Species Comparison ◽  
Citrus Orchard ◽  
A New Species

AbstractA new entomopathogenic nematode in the genus Heterorhabditis is described from South Africa, from two singular isolates found 1000 km from each other, from beneath a fig tree and in a citrus orchard, respectively. Morphological and molecular studies indicate both isolates to be the same and a new undescribed Heterorhabditis species. Comparison of sequences of the internal transcribed spacer (ITS) rDNA and the D2D3 region of the 28S rDNA gene with available sequences of other described species within the genus, indicate the two isolates as a new species. Phylogenetic analysis of the sequence data concerned placed the new species, H. noenieputensis n. sp., closest to H. indica and H. gerrardi in the indica-group. The new species, H. noenieputensis n. sp., is distinguished from other species in the genus by a combination of several morphological traits of the males and the infective juveniles (IJs). The new species differs from all other species previously described, as regards the body length of the IJs, except for H. indica and H. taysearae, in which the IJ is smaller. The IJ also differs from that of H. indica in the length of the oesophagus, the body diameter, the length of the tail and the E%. In addition, males of H. noenieputensis n. sp. differ from their closest relative, H. indica, in the position of the excretory pore, SW% and D%; and from H. gerrardi in the length of the oesophagus and SW%. The seventh pair of genital papillae of H. noenieputensis n. sp. are normally developed, while for H. indica they are often branched or swollen at the base, while 8 and 9 are usually absent in both species.

scholarly journals A new species of Eurytoma (Hymenoptera: Eurytomidae) attacking Quadrastichus spp. (Hymenoptera: Eulophidae) galling Erythrina spp. (Fabaceae), with a summary of African Eurytoma biology and species checklist

Zootaxa ◽  
2008 ◽  
Vol 1751 (1) ◽  
pp. 1 ◽  
Author(s):  
M. GATES ◽  
G. DELVARE
Keyword(s):  
South Africa ◽  
New Species ◽  
New Combinations ◽  
African Species ◽  
Species Checklist ◽  
A New Species ◽  
Host Associations

Eurytoma erythrinae Gates and Delvare, new species, is described and illustrated. This species was reared from fieldcollected galls on Erythrina spp. (Fabaceae) induced by Quadrastichus spp. (Hymenoptera: Eulophidae), in Tanzania, Ghana, and South Africa. It is compared with very similar African species, Eurytoma radicicola Risbec. Afrotropical species classified in Eurytoma are reviewed and twenty-seven new combinations are proposed: Aximopsis acaciacola (Hedqvist) comb. n., A. caryedocida (Rasplus) comb. n., A. lamtoensis (Rasplus) comb. n., A. mateui (Hedqvist) comb. n., A. mimosarum (Rasplus) comb. n., A. obocki (Risbec) comb. n., A. oryzivora (Delvare) comb. n., A. saharensis (Hedqvist) comb. n., A. senegalensis (Risbec) comb. n., A. tropicana (Risbec) comb. n., Bruchophagus conapionis (Rasplus) comb. n., Fronsoma ellenbergeri (Risbec) comb. n., Gibsonoma amborasahae (Risbec) comb. n., G. aphloiae (Risbec) comb. n., G. bararakae (Risbec) comb. n., G. eugeniae (Risbec) comb. n., G. mandrakae (Risbec) comb. n., G. pauliani (Risbec) comb. n., G. plectroniae (Risbec) comb. n., G. tavolae (Risbec) comb. n., Philolema arachnovora (Hesse) comb. n., P. arnoldi (Waterston) comb. n., P. bambeyi (Risbec) comb. n., P. braconidis (Ferrière) comb. n., and P. syleptae (Ferri_re) comb. n., Phylloxeroxenus cressoni (Howard) comb. n., and Sycophila plectroniae (Risbec) comb. n. Lectotypes are designated for ten species: Eurytoma perineti Risbec, E. radicicola Risbec, E. toddaliae Risbec, Gibsonoma amborasahae (Risbec), G. aphloiae (Risbec), G. eugeniae (Risbec), G. mandrakae (Risbec), G. pauliani (Risbec), G. tavolae (Risbec), and P. bambeyi (Risbec). We also provide a checklist of Afrotropical species that are or were classified in Eurytoma and tabulate the known host/associations of these species with references.

Tremateia murispora sp. nov. (Didymosphaeriaceae, Pleosporales) from Guizhou, China

Phytotaxa ◽  
2019 ◽  
Vol 416 (1) ◽  
pp. 79-87 ◽  
Author(s):  
YAO FENG ◽  
SHENG-NAN ZHANG ◽  
ZUO-YI LIU
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Monophyletic Clade ◽  
Distinct Lineage ◽  
A New Species

An Ascomycetous species was found from decaying woody stems in Guizhou, China. Morphologically, the fungus can be assigned well in Tremateia in having immersed ascomata, cellular pseudoparaphyses and muriform ascospores, but it differs from other species in having the 3–7 transverse septa ascospores surrounded by a gelatinous sheath. Phylogenetic analyses based on a concatenated dataset of ITS, LSU, SSUand TEF1-α sequence data indicated that the fungus belongs to the genus Tremateia, Didymosphaeriaceae, four taxa formed a monophyletic clade and represent the genus Tremateia with well supported. The new collection formed a distinct lineage and can be recognized as a new species. Therefore, a new species Tremateia murispora is introduced with detailed description and illustration, the notes on its morphologically and phylogenetically related taxa is provided.

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