scholarly journals Systematic position of the genus Atetylenchus Khan, 1973 (Nematoda: Tylenchidae) with description of two new species

Nematology ◽  
2020 ◽  
Vol 22 (10) ◽  
pp. 1155-1167
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Pablo Castillo ◽  
Juan E. Palomares-Rius ◽  
Reza Ghaderi
Keyword(s):  
Anterior Part ◽  
Molecular Data ◽  
Systematic Position ◽  
Ribosomal Genes ◽  
The Body ◽  
28S Rrna ◽  
Khuzestan Province ◽  
Two New Species ◽  
Tail Shape ◽  
Partial 18S

Summary Two new and one known species of Atetylenchus recovered from Dezful, Khuzestan Province, south-western Iran, are illustrated and described using morphological, morphometric and molecular data. Atetylenchus dezfuliensis n. sp. is characterised by medium-sized body 700-998 μm long, stylet 10.0-11.5 μm long, characteristic tail shape, first tapering regularly but then cylindrical to slightly clavate and 90-136 μm long, phasmids located at one-third anterior part of tail and short spicules 18.7 μm long. Atetylenchus longilabiatus n. sp. is characterised by small-sized body 593-720 μm long, prominent lip region, stylet 9.5-12.5 μm long, conical tail with hook-shaped terminus and 42-63 μm long, phasmids located near mid-tail and short spicules 17.5 μm long. Atetylenchus cf. graminus is characterised by small-sized body 643-787 μm long, stylet 10.7-11.7 μm long, vulva located at 51.7-55.1% of the body length, and conical tail with finely rounded end and 63-69 μm long. Molecular data are provided for the characterisation of this genus from these two new and one known species using ribosomal genes (18S and 28S rRNA). This genus is molecularly separated from Psilenchus, but closely related in both genes. These molecular markers showed different phylogenies for both genera; partial 18S showed their relationship with Tylenchidae, while 28S rRNA showed their relationship with Merliniidae. A key to the genus is provided for species identification.

scholarly journals Taxonomical considerations and molecular phylogeny of the closely related genera Bitylenchus, Sauertylenchus and Tylenchorhynchus (Nematoda: Telotylenchinae), with one new and four known species from Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
M. Hosseinvand ◽  
A. Eskandari ◽  
S. Ganjkhanloo ◽  
R. Ghaderi ◽  
P. Castillo ◽  
...  
Keyword(s):  
New Species ◽  
Anterior Part ◽  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Molecular Data ◽  
28S Rrna ◽  
Natural Habitats ◽  
Morphometric Characters ◽  
Molecular Approaches ◽  
Tail Tip

Abstract During several nematological surveys in cultivated and natural habitats in Khuzestan and Zanjan provinces of Iran, a new species, Bitylenchus parvulus n. sp., two new records for Iran – namely, Tylenchorhynchus agri and Tylenchorhynchus graciliformis – and a population of Bitylenchus parvus and one of Sauertylenchus maximus were recovered and characterized based upon morphological and molecular approaches. The new species is characterized by lip region with five to seven annuli, stylet 17.7 (17.0–18.5) μm long, sub-cylindrical tail narrowing abruptly near terminus giving a bluntly digitate shape to the tail tip, cuticle near anterior part of vulva wrinkled and post-rectal sac occupies whole of tail cavity. The phylogenetic analyses were carried out using molecular data from D2–D3 expansion segments of large ribosomal subunit (28S rRNA) for all studied species and the partial small ribosomal subunit (18S rRNA) for the new species. The representatives of Bitylenchus and Sauertylenchus formed distinct clades from Tylenchorhynchus members, supporting the hypothesis in which Bitylenchus and Sauertylenchus could be considered as valid genera, but rejecting the ‘large-genus’ concept for Tylenchorhynchus. Also, Sauertylenchus ibericus was proposed as a junior synonym of S. maximus based on the results from morphological and phylogenetic analysis. Furthermore, an identification key for all known species included in the three genera Bitylenchus, Tylenchorhynchus and Sauertylenchus is presented herein. The number of transverse annuli on the lip region and presence/absence of post-rectal sac were considered as the main diagnostic characters for classifying the species into seven groups, and other morphological and morphometric characters were subsequently used for distinguishing species in each group.

scholarly journals Comparative morphometrics and ribosomal DNA sequence analysis of Longidorus orientalis Loof, 1983 (Nematoda: Longidoridae) from Spain and Iran

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 631-640 ◽  
Author(s):  
Juan E. Palomares-Rius ◽  
Blanca B. Landa ◽  
Zahra Tanha Maafi ◽  
David J. Hunt ◽  
Pablo Castillo
Keyword(s):  
18S Rrna ◽  
Date Palm ◽  
Original Description ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Khuzestan Province ◽  
South West ◽  
Graminaceous Plants ◽  
Partial 18S ◽  
Ribosomal Dna Sequence

Abstract During recent nematode surveys in a muddy soil around undetermined graminaceous plants in El Rocío, Huelva Province, in southern Spain, and from the rhizosphere of date palm associated with graminaceous vegetation from Abadan, in Khuzestan Province, south-west Iran, populations of Longidorus orientalis were identified. Morphological and morphometrical studies on these populations fit the original description and represent the first report from Spain and Europe. Molecular characterisation of L. orientalis from Spain and Iran, using D2-D3 expansion regions of 28S rRNA, 18S rRNA and ITS-rRNA, is provided. Sequences of the D2-D3 expansion regions and partial 18S genes were analysed using maximum likelihood and Bayesian inference to reconstruct phylogenetic relationships within L. orientalis and other Longidorus species. The results revealed a closer phylogenetic relationship with L. goodeyi for the D2-D3 expansion region and with L. vineacola for the partial 18S region.

scholarly journals Integrative taxonomy reveals two new cryptic species of Hyphessobrycon Durbin, 1908 (Teleostei: Characidae) from the Maracaçumé and middle Tocantins River basins, Eastern Amazon region

2020 ◽  
Vol 723 ◽  
pp. 77-107
Author(s):  
Erick Cristofore Guimarães ◽  
Pâmella Silva De Brito ◽  
Pedro Henrique Negreiros Bragança ◽  
Jadson Pinheiro Santos ◽  
Axel Makay Katz ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Morphological Characters ◽  
Molecular Data ◽  
Color Pattern ◽  
Integrative Taxonomy ◽  
The Body ◽  
Dorsal Fin ◽  
Two New Species ◽  
Molecular Phylogenetic

Two new species, Hyphessobrycon frickei Guimarães, Brito, Bragança, Katz & Ottoni sp. nov. and H. geryi Guimarães, Brito, Bragança, Katz & Ottoni sp. nov., are herein described, based on seven different and independent species delimitation methods, and on molecular and morphological characters, making the hypothesis of these new species supported from an integrative taxonomy perspective. They belong to the “Rosy tetra” clade, which is mainly characterized by the presence of a dark brown or black blotch on the dorsal fin and the absence of a midlateral stripe on the body. These two new species are distinguished from the other members of this clade mainly by the arrangement, shape and color pattern of humeral and dorsal-fin spots, as well as by other characters related to scale counts and body pigmentation. The placement of the new species within the “Rosy tetra” clade was based on the combination of morphological character states mentioned above and corroborated by a molecular phylogenetic analysis using the mitochondrial gene cytochrome oxidase subunit 1. In addition, a new clade (here termed Hyphessobrycon copelandi clade) within the “Rosy tetra” clade is proposed based on molecular data, comprising H. copelandi, H. frickei sp. nov., H. geryi sp. nov. and a still undescribed species. Our results corroborate the occurrence of hidden species within the “Rosy tetra” clade, as suggested by previous studies.

Studies on Longidorus iberis (Escuer & Arias, 1997) n. comb. (Nematoda: Longidoridae) from Spain

Nematology ◽  
2021 ◽  
pp. 1-15
Author(s):  
Ilenia Clavero-Camacho ◽  
Gracia Liébanas ◽  
Miguel Escuer ◽  
Carolina Cantalapiedra-Navarrete ◽  
Antonio Archidona-Yuste ◽  
...  
Keyword(s):  
Molecular Markers ◽  
18S Rrna ◽  
Molecular Data ◽  
Valid Species ◽  
28S Rrna ◽  
Closely Related Species ◽  
North West ◽  
Molecular Studies ◽  
First Time ◽  
Partial 18S

Summary Specimens of a thin longidorid species collected in Peñalba (Huesca), north-west Spain, were previously described as Paralongidorus iberis. However, we conclude, through scanning electron microscopy and molecular studies on a population from about 15 km from the type locality and on paratype specimens, that this species was originally placed in the wrong genus. Both populations have pore-like amphidial apertures, not slit-like as in Paralongidorus, and the species is therefore transferred to Longidorus. Longidorus iberis n. comb. is regarded as a valid species and is clearly different from closely related species such as L. tabernensis, L. iliturgiensis, L. alvegus and L. indalus in morphometrics and molecular markers. Molecular data are reported for the first time, including the D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, partial 18S rRNA and partial mitochondrial coxI regions. These molecular markers were used for inferring the phylogenetic relationships with other species within Longidorus and Paralongidorus, all clearly separating L. iberis n. comb. from other related taxa and placing the species in the Longidorus clade, rather than with Paralongidorus.

Morphological and molecular characterisation of Coslenchus persicus n. sp. (Nematoda: Tylenchidae) from Iran

Nematology ◽  
2020 ◽  
pp. 1-10
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Ribosomal Genes ◽  
28S Rrna ◽  
Lateral Field ◽  
Rrna Tree ◽  
Basal Bulb ◽  
Stylet Length ◽  
North Western ◽  
A New Species

Summary A new species of Coslenchus from north-western Iran is illustrated and described using morphological, morphometric and molecular data. Coslenchus persicus n. sp. is characterised by a medium-sized body of 694-810 μm, often 18 (seldom 20-22) longitudinal ridges excluding lateral field, head with four distinct annuli, stylet 9.6-10.8 μm long, pharyngeal basal bulb large and cylindrical, spermatheca offset and ovate, without sperm, vulval flaps extending for two annuli, 5-7 μm in length, tail striated with finely to bluntly rounded tip, 110-153 μm long and without males. The new species comes close to C. assamensis, C. diversus, C. japonicus, C. leiocephalus, C. maritus, C. multigyrus, C. paramaritus, C. pastor, and C. polonicus, but can be distinguished from all by differences in body length, stylet length and shape of basal bulb, post-vulval uterine sac, tail and certain morphometric data. Molecular data are provided for the characterisation of the new species using ribosomal genes (18S and 28S rRNA). In the 18S rRNA tree, the new species was placed in a clade including one isolate of Coslenchus sp. from Iran. In the reconstructed 28S rRNA phylogenetic tree, C. persicus n. sp. clustered with other known Coslenchus species.

scholarly journals Molecular and morphological characterisation of Longidorus polyae sp. n. and L. pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria

ZooKeys ◽  
2019 ◽  
Vol 830 ◽  
pp. 75-98 ◽  
Author(s):  
Stela S. Lazarova ◽  
Milka Elshishka ◽  
Georgi Radoslavov ◽  
Lydmila Lozanova ◽  
Peter Hristov ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Nematode Species ◽  
Molecular Data ◽  
18S Rrna Gene ◽  
The Body ◽  
Integrative Approach ◽  
Rrna Gene ◽  
28S Rrna ◽  
Stage Juvenile

Longidoruspolyaesp. n., a bisexual nematode species found in the rhizosphere of pear tree (Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8–9.1 mm; a comparatively long odontostyle (114.0–127.5 μm); a narrow lip region (14.0–15.5 μm), anteriorly flattened and almost continuous with the body profile; pocket-like amphidial pouches long, deeply bilobed, and slightly asymmetrical, a guide ring at 37–42 μm from the anterior end; normal arrangement of pharyngeal glands; and a short bluntly rounded to hemispherical tail. Four juvenile stages identified: the first stage with a digitate tail, and the second and subsequent stages with a bluntly rounded tail. Males have one adcloacal pair and a row of 10 or 11 single ventromedian supplements; spicules 71.0–74.5 μm long. Based on morphometric data, the new species belongs to a group of species spread over Europe (L.arthensis, L.silvae, L.uroshis,), Iran (L.kheirii), and Syria (L.pauli), which share common characters such as amphidial fovea, lip region and tail shapes, similar odontostyle and body length, and similar first-stage juvenile tail shape. Codes for identifying the new species are A5, B2, C34, D3, E3, F45, G12, H1, I2, J1, K7. The phylogenetic analysis based on D2-D3 expansion domains of the rRNA gene revealed that the new species has the closest relationships with L.athesinus from Italy and three unidentified Longidorus spp. from USA (Longidorus sp. 1, Longidorus sp. 2, and Longidorus sp. 6). New morphometric and molecular data (18S rRNA gene, ITS1-5.8S-ITS2 regions and D2-D3 28S rRNA gene sequences) for three populations of L.pisi from Bulgaria were obtained and variations between populations are discussed.

A new dagger nematode, Xiphinema poasense n. sp. (Nematoda: Longidoridae), from Costa Rica

Nematology ◽  
2018 ◽  
Vol 20 (3) ◽  
pp. 235-252 ◽  
Author(s):  
Ingrid Varela-Benavides ◽  
Walter Peraza-Padilla ◽  
Carolina Cantalapiedra-Navarrete ◽  
Juan E. Palomares-Rius ◽  
Pablo Castillo ◽  
...  
Keyword(s):  
Costa Rica ◽  
18S Rrna ◽  
Mitochondrial Gene ◽  
The Body ◽  
Wild Plants ◽  
Rrna Gene ◽  
28S Rrna ◽  
Body Contour ◽  
Genital Branch ◽  
Partial 18S

A new dagger nematode,Xiphinema poasensen. sp., is described and illustrated from three populations extracted from soil associated with a combined plantation ofEucalyptussp.,Cupressussp. andPennisetumsp. and wild plants from a tropical pre-montane forest in Costa Rica. The new dagger nematode is characterised by a moderate body size 2612 (2416-3042) μm long, a rounded lip region 15.0 (13.5-16.5) μm broad, separated from the body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a very long odontostyle (175 (164-188) μm), stylet guiding ring located 167 (136-181) μm from anterior end, vulva situated anterior to mid-body (36-40%), anterior genital branch complete but strongly reduced, without uterine differentiation, female tail short, hemispherical to convex-conoid with a c′ ratio = 0.7 (0.6-0.8) and bearing two pairs of caudal pores, and male absent. Integrative diagnosis was completed with molecular data using D2-D3 expansion segments of 28S rRNA, ITS1 region, partial 18S-rRNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships based on D2-D3 segments of this species with otherXiphinemaspp. of theX.non-americanumgroup indicated thatX. poasensen. sp. clustered with other species with a reduced anterior genital branch from the morphospecies Group 2,viz.,X. costaricenseandX. krugi. However, the phylogeny ofcoxIand partial 18S rRNA gene revealed that the new species did not cluster withXiphinemaspecies having the anterior genital branch absent or reduced (i.e., morphospecies Groups 1 and 2, respectively).

Two new species and three morphospecies of Ficophagus Davies & Bartholomaeus, 2015 (Nematoda: Aphelenchoididae) from Ficus subgenus Pharmacosycea (Moraceae) in Central America

Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 351-374 ◽  
Author(s):  
Kerrie A. Davies ◽  
Weimin Ye ◽  
Barbara Center ◽  
Natsumi Kanzaki ◽  
Faerlie Bartholomaeus ◽  
...  
Keyword(s):  
New Species ◽  
Central America ◽  
Dna Sequence ◽  
Sequence Data ◽  
Morphological Characters ◽  
Molecular Data ◽  
Dna Sequence Data ◽  
Female Tail ◽  
Two New Species ◽  
Tail Shape

Aphelenchoidid nematodes were collected from the sycones ofFicussubgenusPharmacosyceafrom Central America. Two new species ofFicophaguswere recovered, and are described herein asF. maximasp. n. andF. yoponensissp. n. fromFicus maximaandF. yoponensis, respectively. They are differentiated from other species of the genus by a combination of morphological characters including having C-shaped females and spiral males, EP opening close to the lips, a short to long post-uterine sac, spicule characters, three pairs of subventral caudal papillae, DNA sequence data, biogeographical range, and host wasp andFicusspecies affiliation. The new species are differentiated from each other by spicule characters, length of the post-uterine sac, spermatheca shape, and female tail shape. In addition, three morphospecies were collected from sycones ofFicus glabrata,F. insipidaandF. tonduzii, respectively. Their morphological descriptions are presented but these taxa are not formally named as they currently lack molecular data.

scholarly journals Distribution of trichodorid species in mainland China with description of Trichodorus hangzhouensis sp. nov. (Nematoda, Triplonchida)

ZooKeys ◽  
2020 ◽  
Vol 945 ◽  
pp. 163-189
Author(s):  
Xuqing Li ◽  
Munawar Maria ◽  
Ruihang Cai ◽  
Eda Marie Barsalote ◽  
Vlada Peneva ◽  
...  
Keyword(s):  
Ventral View ◽  
Anterior Part ◽  
Mainland China ◽  
Molecular Data ◽  
Extensive Study ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Widespread Species ◽  
Pharyngeal Bulb

Seven trichodorid species including a new one (Trichodorus hangzhouensissp. nov., T. nanjingensis, T. pakistanensis, T. cedarus, Paratrichodorus porosus, Nanidorus renifer and N. minor) were recovered from the rhizosphere of different hosts in 13 provinces of China. Each of the recovered species was characterized based on morphology and molecular data using rRNA gene sequences. Trichodorus hangzhouensissp. nov. is characterized by its males having medium-sized onchiostyle (46–49 µm) and three ventromedian cervical papillae (CP) anterior to the secretory-excretory (S-E) pore, CP1 located opposite the anterior part of isthmus, S-E pore opposite the isthmus or anterior end of pharyngeal bulb, spicules slightly ventrally curved, relatively small, 33.2 (32.0–34.5) µm long, wider slightly marked capitulum, lamina partially striated without bristles at striation; and females having rounded triangular sclerotized vaginal pieces with tips directed towards vulva, 1.5–2.0 µm sized, at about 1 µm apart, vulva pore-like in ventral view. Phylogenetic analysis based on D2-D3 28S rRNA gene sequences differentiated the new species among Trichodorus species from Europe, Asia and USA which formed a large clade. A review of the distribution of Trichodorus, Nanidorus and Paratrichodorus species revealed that T. cedarus, T. nanjingensis, T. pakistanensis and P. porosus are the most widespread species recorded from different provinces of China. This is the first extensive study of trichodorid species occurring in China.

scholarly journals Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverflies species

2019 ◽  
Vol 89 (1) ◽  
pp. 74-125 ◽  
Author(s):  
Laura Likov ◽  
Ante Vujić ◽  
Nataša Kočiš Tubić ◽  
Mihajla Đan ◽  
Nevena Veličković ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Systematic Position ◽  
Species Group ◽  
Rrna Genes ◽  
Coi Gene ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Diagnostic Key ◽  
Morphological And Molecular Data

The putative monophyly and systematic position of Merodon nigritarsis group was assessed based on morphological and molecular data of the mitochondrial COI and nuclear 28S rRNA genes. The previously reported concept of the group has been redefined, and M. crassifemoris Paramonov, 1925 is now excluded. The related M. avidus group is redefined here, including the Merodon avidus complex and M. femoratus Sack, 1913. Species delimitation of morphologically defined species of M. nigritarsis group was well supported by COI gene analysis, with the exception of M. alagoezicus Paramonov, 1925 and M. lucasi Hurkmans, 1993. Descriptions are given for three new species of the M. nigritarsis species group: Merodon cohurnus Vujić, Likov et Radenković sp. n., Merodon longisetus Vujić, Radenković et Likov sp. n. and Merodon obstipus Vujić, Radenković et Likov sp. n., and one new species from the M. avidus group: Merodon rutitarsis Likov, Vujić et Radenković sp. n. A lectotype is designated for M. femoratus Sack, 1913, and two new synonymies of this species were proposed: M. biarcuatus Curran, 1939 and M. elegans Hurkmans, 1993. Here we review 18 species from the M. nigritarsis group and six species from the M. avidus group and provide morphological diagnoses of the species groups. Additionally, diagnosis of 12 branches (groups or individual taxa) of M. avidus-nigritarsis lineage, an illustrated diagnostic key for the males, and distribution map are provided for the new species.

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