Ottolenchus sinipersici n. sp. (Rhabditida: Tylenchidae) from the Persian Gulf mangrove forests, Iran

Summary A new species of Tylenchidae from the rhizosphere of mangrove trees in Hormozgan and Khuzestan provinces, Iran, is described based on morphological and molecular data. Ottolenchus sinipersici n. sp., is characterised by a slightly fusiform body 560-665 μm long, lateral field in the form of a narrow band with two faint incisures that are not visible in fatter females, indistinct transverse annuli under the light microscope, cephalic region continuous with the body contour, smooth and flattened dorsoventrally, longitudinal and narrow sigmoid amphidial slits, stylet delicate, 10.1-11.2 μm long, with small rounded to slightly posteriorly sloping knobs, well-developed median bulb, offset and pyriform pharyngeal basal bulb, vulva located at 66.9-69.6% of body length, offset spermatheca, short post-vulval uterine sac, spicules 18.5-20.5 μm long with highly curved blades, and a 113-135 μm long filiform tail with a hook-like or coiled terminus. In Bayesian inference phylogenetic trees based on the partial small subunit ribosomal DNA (SSU rDNA) and D2-D3 expansion segment of large subunit ribosomal DNA (D2-D3 LSU rDNA) genes, the new species clustered together with O. facultativus (KJ869310) in SSU, and forms a clade with three isolates of O. discrepans in LSU phylogeny. Ottolenchus fungivorus n. comb. (= Filenchus fungivorus) is proposed.

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Description of Ektaphelenchus cupressi n. sp. (Nematoda: Ektaphelenchinae) from Iran

Nematology ◽

10.1163/15685411-00003245 ◽

2019 ◽

Vol 21 (7) ◽

pp. 679-691 ◽

Cited By ~ 1

Author(s):

Behrouz Golhasan ◽

Mehdi Abdollahpour ◽

Yiwu Fang ◽

Joaquín Abolafia ◽

Ramin Heydari

Keyword(s):

New Species ◽

Large Subunit ◽

Small Subunit ◽

The Body ◽

Cupressus Sempervirens ◽

Mazandaran Province ◽

Head Region ◽

Northern Iran ◽

Body Contour ◽

Lateral Field

Summary Ektaphelenchus cupressi n. sp. was isolated during a survey of nematodes associated with bark samples of a dead cypress tree (Cupressus sempervirens) in Mazandaran province, northern Iran. The new species is characterised by a female body length of 612 (520-693) μm, stylet 17-19 μm long with wide lumen and lacking basal swellings, head region hemispherical in lateral view and slightly offset from the body contour by a shallow constriction, three incisures in the lateral field, excretory pore situated anterior to level of metacorpus valve, hemizonid not seen, post-uterine sac 29-35 μm long, shape of the tail terminus, and arrangement of the male caudal papillae. By a combination of morphological characters, e.g., stylet without knobs or swellings at the base, short conical tail, PUS length, and only a vestigial anus and rectum in most individuals, the new species shares similarities with species belonging to three genera, i.e., Anomyctus, Ektaphelenchus, and Seinura. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rDNA confirmed its status as a new species.

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Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽

10.11646/zootaxa.4369.3.6 ◽

2018 ◽

Vol 4369 (3) ◽

pp. 406 ◽

Cited By ~ 3

Author(s):

MAJID PEDRAM ◽

MAHYA SOLEYMANZADEH ◽

EBRAHIM POURJAM ◽

MAHYAR MOBASSERI

Keyword(s):

New Species ◽

Large Subunit ◽

Small Subunit ◽

Phylogenetic Study ◽

Molecular Characteristics ◽

Lsu Rdna ◽

Northern Iran ◽

Lateral Field ◽

Molecular Phylogenetic ◽

Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree.

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Description of Ektaphelenchus masseyi n. sp. (Rhabditida: Ektaphelenchinae) found in dead wood, from Iran

Nematology ◽

10.1163/15685411-bja10057 ◽

2020 ◽

pp. 1-12

Author(s):

Fariba Heydari ◽

Majid Pedram

Keyword(s):

New Species ◽

Large Subunit ◽

The Body ◽

Body Region ◽

Lsu Rdna ◽

Similar Species ◽

Natural Forests ◽

Lateral Field ◽

Rotten Wood ◽

Lateral Lines

Summary Ektaphelenchus masseyi n. sp. is described and illustrated. The new species was isolated from dead/rotten wood collected from natural forests in north Iran. It is characterised by 501-758 μm long females having three lines in lateral field, more or less continuous cephalic region separated from the body only by a shallow depression, relatively short tripartite stylet (total length 13-14 μm) lacking basal knobs or swellings, excretory pore at base of metacorpus or slightly anterior to it, post-vulval uterine sac 24-32 μm long, rectum and anus absent, posterior body region conical with finely rounded terminus, and males rare, with a dorsally convex and ventrally flat tail with a small mucron at terminus, 13-15 μm long spicules, and seven caudal papillae. The new species was morphologically compared with typologically similar species of Ektaphelenchus having three lines in the lateral field, namely E. joyceae, E. macrobulbosus, E. taiwanensis, and E. winteri. It was further compared with E. olitorius and E. prolobos having a similar female posterior body end shape and with an unknown number of incisures in the lateral field. It was also compared with three species currently placed under Ektaphelenchoides, namely E. andrassyi and E. poinari with three lines in the lateral field, and E. compsi with obscure lateral lines. The molecular phylogenetic relationships of the new species with other ektaphelenchid species were investigated using partial small, and large subunit ribosomal RNA gene (SSU and LSU rDNA D2-D3) sequences using the maximal number of currently sequenced ektaphelenchid and seinurid taxa.

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Molecular and morphological characterisation of Paurodontella persica n. sp. (Hexatylina: Sphaerulariidae) from soil in Iran

Nematology ◽

10.1163/15685411-00003031 ◽

2017 ◽

Vol 19 (1) ◽

pp. 57-68 ◽

Cited By ~ 2

Author(s):

Mehrab Esmaeili ◽

Ramin Heydari ◽

Weimin Ye

Keyword(s):

New Species ◽

Large Subunit ◽

Small Subunit ◽

Body Contour ◽

Pharyngeal Bulb ◽

Lateral Field ◽

Rdna Gene ◽

Posterior Extension ◽

Large Subunit Rdna ◽

A New Species

A new species ofPaurodontella,P. persican. sp., collected from the Alborz rangelands of Iran, is described and illustrated. The new species is characterised by its body length of 506 (416-599) μm and 424 (388-461) μm in female and male, respectively, four incisures in lateral field, lip region annulated and continuous with body contour, total stylet 6.2 (5.5-7.0) μm long. Basal pharyngeal bulb with small posterior extension projecting reaching to the intestine. Excretory pore situated at the level of basal pharyngeal bulb region. Post-uterine sac short, 4.5 (3.0-6.0) μm long and uterus with an offset diverticulum. Tail of both sexes similar, conoid pointed, terminus tapering into a long pointed mucron. Male with adanal bursa and spicules 16.5 (16.0-17.0) μm long. The new species comes close in morphology and morphometrics to four known species of the genus, namelyP. auriculata,P. myceliophaga,P. minutaandP. niger. Molecular analyses of the partial small subunit rDNA gene (SSU), D2-D3 expansion segments of the large subunit rDNA gene (LSU) and internal transcribed spacer (ITS) revealed this to be a new species.

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Description of Ektaphelenchus taiwanensis sp. n. (Nematoda: Ektaphelenchinae) found in packaging wood from Taiwan

Nematology ◽

10.1163/15685411-00002676 ◽

2013 ◽

Vol 15 (3) ◽

pp. 329-338 ◽

Cited By ~ 6

Author(s):

Jianfeng Gu ◽

Jiangling Wang ◽

Xianfeng Chen

Keyword(s):

New Species ◽

Phylogenetic Trees ◽

Morphological Characters ◽

Feeding Habit ◽

The Body ◽

Body Contour ◽

Lateral Field ◽

Male Tail

Ektaphelenchus taiwanensis sp. n. is described and figured. The new species was isolated from pine packaging wood from Taiwan and inspected in Ningbo harbour, China, in 2009. Ektaphelenchus taiwanensis sp. n. is characterised by the lip region lacking a clear constriction separating it from the body contour, stylet relatively short (12-17 μm) and without basal knobs, and presence of three lines in the lateral field. The female post-uterine sac is less than a body diam. long, the rectum and anus are absent and the tail is conoid with a sharply pointed terminus. The male tail is conoid and has a hair-like mucron (0.5-2.5 μm), the spicules are mitten-shaped, 12.5-14.4 μm long (chord) and are smoothly curved with a prominent rounded condylus, prominent pointed rostrum, and broadly rounded distal end. There are three pairs of subventral caudal papillae. Ektaphelenchus taiwanensis sp. n. is similar to E. betulae, E. josephi, E. joyceae, E. prolobos, E. propora and E. tuerkorum from which it can be separated by a combination of various morphometric and morphological characters. The D2D3 LSU and partial SSU region sequences were analysed and aligned using ClustalW implemented in MEGA version 4.0. Phylogenetic trees were generated with the Neighbour Joining (NJ) method using the Tajima-Nei distance option. The feeding habit of the new species is briefly discussed.

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Ektaphelenchoides shiroodensis n. sp. (Rhabditida: Ektaphelenchinae) a new member of the genus

Nematology ◽

10.1163/15685411-00003318 ◽

2020 ◽

Vol 22 (5) ◽

pp. 493-503 ◽

Cited By ~ 1

Author(s):

Farzad Aliramaji ◽

Ebrahim Pourjam ◽

Majid Pedram

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

The Body ◽

Body Region ◽

Lsu Rdna ◽

Similar Species ◽

Mazandaran Province ◽

Lateral Field ◽

Posterior Body Region

Summary Ektaphelenchoides shiroodensis n. sp. is described and illustrated based upon morphological, morphometric and molecular data. It was recovered from the bark samples of a dead alder tree (Alnus sp.) from countryside around Shirood city, Mazandaran province, in the north of Iran. The new species is characterised by 768-985 μm long females, its lip region separated from the body contour by constriction, lateral field with three barely visible lines, forming two weak bands, 26-29 μm long stylet with wide lumen without conophore and basal swellings, excretory pore always at the level with median bulb, post-vulval uterine sac (PUS) 43-76 μm long, elongate conoid posterior body region ending to a long filiform part with pointed tip, males common with dorsally convex conical tail ending to a short narrower region with pointed tip, seven precloacal + cloacal + caudal papillae and arcuate spicules with wide condylus, blunt rostrum and simple end. Based upon the similar posterior body region (‘tail’) and comparable PUS length, the new species was compared to three known species, viz., E. attenuata, E. hunti and E. pini. Comparisons with the aforementioned species and similar species under the genus Seinura are discussed. The phylogenetic affinities of the new species, based upon two partial small and large subunit ribosomal DNA (SSU and LSU rDNA D2-D3) sequences, are discussed. Furthermore, several ektaphelenchid and seinurid species previously described from Iran were recovered in the present study, sequenced for their aforementioned genomic markers, and included in the phylogenetic analyses.

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First record of the genus Sakia Khan, 1964 (Nematoda: Tylenchidae) from Iran, with proposal of Sakia arboris n. sp.

Nematology ◽

10.1163/15685411-00003150 ◽

2018 ◽

Vol 20 (5) ◽

pp. 449-460 ◽

Cited By ~ 2

Author(s):

Yousef Panahandeh ◽

Ebrahim Pourjam ◽

Ali Roshan-Bakhsh ◽

Majid Pedram

Keyword(s):

New Species ◽

Ribosomal Dna ◽

18S Rdna ◽

Phylogenetic Analyses ◽

Large Subunit ◽

First Record ◽

Small Subunit ◽

28S Rdna ◽

Northern Iran ◽

Body Contour

Summary The genus Sakia is recorded from Iran for the first time and Sakia arboris n. sp., recovered from two geographical points in northern Iran, is described. The new species is characterised by its smooth cuticle under light microscopy (LM), absence of lateral fields, head continuous with body contour, flattened lip region, 9.3 (9.0-10.0) μm long stylet, vulva at 60.2 (59.3-61.3)%, bilobed spermatheca, 117 (102-128) μm long tail, and 14.3 (14-15) μm long spicules. By lacking lateral fields, the new species comes close to three known species of the genus, namely: S. alii, S. castori and S. indica. Molecular phylogenetic analyses using sequences of the near full-length fragment of the small subunit of ribosomal DNA (18S rDNA) and the D2-D3 expansion segments of the large subunit ribosomal DNA (28S rDNA) were performed using Bayesian inference (BI) and maximum likelihood (ML) methods. In the reconstructed Bayesian tree using the 18S rDNA sequence of the type population, the new species occupied a position in a clade including two isolates of Sakia sp. and some species of Lelenchus, with maximal BPP and high ML BS values (1.00/99%). In the reconstructed 28S rDNA phylogenetic tree, two newly sequenced isolates of S. arboris n. sp. formed a well-supported clade with Lelenchus spp.

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Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

Parasites & Vectors ◽

10.1186/s13071-020-04430-6 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Yi-Fan Cao ◽

Hui-Xia Chen ◽

Yang Li ◽

Dang-Wei Zhou ◽

Shi-Long Chen ◽

...

Keyword(s):

New Species ◽

Ribosomal Dna ◽

Dna Sequences ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Small Subunit ◽

Sister Relationship ◽

Tibetan Antelope ◽

A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

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New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran

Nematology ◽

10.1163/15685411-00003267 ◽

2019 ◽

Vol 21 (9) ◽

pp. 937-956 ◽

Cited By ~ 1

Author(s):

Yousef Panahandeh ◽

Ebrahim Pourjam ◽

Sergio Álvarez-Ortega ◽

Farahnaz Jahanshahi Afshar ◽

Majid Pedram

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Large Subunit ◽

Small Subunit ◽

Lsu Rdna ◽

Natural Forests ◽

Electron Microscopic ◽

Pharyngeal Bulb ◽

Ribosomal Rna Gene ◽

Molecular Approaches

Summary During nematological surveys in grasslands and natural forests of north and north-western Iran, three species of Miculenchus, including two new and one known species, were recovered and characterised based upon morphological and molecular approaches. Miculenchus brevisalvus n. sp., the first new species, is mainly characterised by its short females 334-388 μm long and with a short 6.0-7.5 μm long stylet, pyriform to pyriform-elongate pharyngeal bulb, 4-8 μm long post-uterine sac (PUS), offset rounded spermatheca filled with small spheroid sperm, elongate conoid tail 62-83 μm long with a sharp tip, and males with simple cloacal lips. Miculenchus muscus n. sp., the second new species, is characterised by a combination of the following features: body 401-467 μm long, well-developed protuberant labial plate at the anterior end under light microscopy, stylet 7-9 μm long, pyriform pharyngeal bulb, PUS 4-9 μm long, gradually narrowing conical tail 62-74 μm long with a finely pointed or sharp end and bearing several fine bristles at tip, and a male with projecting cloacal lips. Both newly described species were morphologically compared with four currently known species of the genus, viz., M. elegans, M. salmae, M. salvus, and M. tesselatus. Miculenchus salmae was also recovered and reported from Iran for the first time. It is mainly characterised by lacking a PUS and the characteristic vagina shape. Miculenchus muscus n. sp. and M. salmae were both characterised using scanning electron microscopic images, yielding new morphological observations for the genus. All three species are studied for their molecular phylogenetic characters using sequences of near-full length fragments of the small subunit ribosomal RNA gene (SSU rDNA) and the D2-D3 expansion segments of the large subunit ribosomal RNA gene (LSU rDNA D2-D3). In both SSU and LSU phylogenies, all currently sequenced species of Miculenchus formed a monophyletic group with maximal clade support in both Bayesian inference and maximum likelihood analysis.

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Deladenus nitobei n. sp. (Tylenchomorpha: Allantonematidae) isolated from Sirex nitobei (Hymenoptera: Siricidae) from Aomori, Japan, a new member of the siricidicola superspecies

Nematology ◽

10.1163/15685411-00003025 ◽

2016 ◽

Vol 18 (10) ◽

pp. 1199-1217 ◽

Cited By ~ 6

Author(s):

Natsumi Kanzaki ◽

Suguru E. Tanaka ◽

Katrin Fitza ◽

Hajime Kosaka ◽

Bernard Slippers ◽

...

Keyword(s):

New Species ◽

Cryptic Species ◽

Pinus Densiflora ◽

Large Subunit ◽

Small Subunit ◽

Body Cavity ◽

The Body ◽

Malt Extract ◽

Internal Transcribed Spacer Region ◽

Mtcoi Gene

Deladenus nitobein. sp., a parasite of a woodwasp species,Sirex nitobei, is described based on its typological characters and molecular profiles of part of the small subunit D2-D3 expansion segments of the large subunit and internal transcribed spacer region of the ribosomal RNA gene, as well as part of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene. Adult host woodwasps emerging from dead Japanese red pine logs,Pinus densiflora, collected at Aomori, Japan, were dissected and examined for nematode association. The new species was isolated from the body cavity and reproductive system ofS. nitobeias large parasitic females and small parasitic juveniles. The nematodes were cultured successfully on 1.0% malt extract agar medium, inoculated withSirex-associated fungus,Amylostereumareolatum. The mycophagous adult nematodes were characterised by the relative position of the excretory pore, located at 25 (19-28) and 25 (18-30)μm anterior to the hemizonid in the male and female, respectively, and a broad female tail with a rounded distal end. Typologically, the new species forms a cryptic species complex withD. siricidicolaandD. canii. In addition, the new species andD. siricidicolashare the same host wasp, tree and fungal species in Japan. However, the cryptic species can be separated from each other based on the described morphological and molecular sequence differences in the mtCOI gene.

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