Description of Ektaphelenchus masseyi n. sp. (Rhabditida: Ektaphelenchinae) found in dead wood, from Iran

Nematology ◽  
2020 ◽  
pp. 1-12
Author(s):  
Fariba Heydari ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Large Subunit ◽  
The Body ◽  
Body Region ◽  
Lsu Rdna ◽  
Similar Species ◽  
Natural Forests ◽  
Lateral Field ◽  
Rotten Wood ◽  
Lateral Lines

Summary Ektaphelenchus masseyi n. sp. is described and illustrated. The new species was isolated from dead/rotten wood collected from natural forests in north Iran. It is characterised by 501-758 μm long females having three lines in lateral field, more or less continuous cephalic region separated from the body only by a shallow depression, relatively short tripartite stylet (total length 13-14 μm) lacking basal knobs or swellings, excretory pore at base of metacorpus or slightly anterior to it, post-vulval uterine sac 24-32 μm long, rectum and anus absent, posterior body region conical with finely rounded terminus, and males rare, with a dorsally convex and ventrally flat tail with a small mucron at terminus, 13-15 μm long spicules, and seven caudal papillae. The new species was morphologically compared with typologically similar species of Ektaphelenchus having three lines in the lateral field, namely E. joyceae, E. macrobulbosus, E. taiwanensis, and E. winteri. It was further compared with E. olitorius and E. prolobos having a similar female posterior body end shape and with an unknown number of incisures in the lateral field. It was also compared with three species currently placed under Ektaphelenchoides, namely E. andrassyi and E. poinari with three lines in the lateral field, and E. compsi with obscure lateral lines. The molecular phylogenetic relationships of the new species with other ektaphelenchid species were investigated using partial small, and large subunit ribosomal RNA gene (SSU and LSU rDNA D2-D3) sequences using the maximal number of currently sequenced ektaphelenchid and seinurid taxa.

Ektaphelenchoides shiroodensis n. sp. (Rhabditida: Ektaphelenchinae) a new member of the genus

Nematology ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. 493-503 ◽  
Author(s):  
Farzad Aliramaji ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
The Body ◽  
Body Region ◽  
Lsu Rdna ◽  
Similar Species ◽  
Mazandaran Province ◽  
Lateral Field ◽  
Posterior Body Region

Summary Ektaphelenchoides shiroodensis n. sp. is described and illustrated based upon morphological, morphometric and molecular data. It was recovered from the bark samples of a dead alder tree (Alnus sp.) from countryside around Shirood city, Mazandaran province, in the north of Iran. The new species is characterised by 768-985 μm long females, its lip region separated from the body contour by constriction, lateral field with three barely visible lines, forming two weak bands, 26-29 μm long stylet with wide lumen without conophore and basal swellings, excretory pore always at the level with median bulb, post-vulval uterine sac (PUS) 43-76 μm long, elongate conoid posterior body region ending to a long filiform part with pointed tip, males common with dorsally convex conical tail ending to a short narrower region with pointed tip, seven precloacal + cloacal + caudal papillae and arcuate spicules with wide condylus, blunt rostrum and simple end. Based upon the similar posterior body region (‘tail’) and comparable PUS length, the new species was compared to three known species, viz., E. attenuata, E. hunti and E. pini. Comparisons with the aforementioned species and similar species under the genus Seinura are discussed. The phylogenetic affinities of the new species, based upon two partial small and large subunit ribosomal DNA (SSU and LSU rDNA D2-D3) sequences, are discussed. Furthermore, several ektaphelenchid and seinurid species previously described from Iran were recovered in the present study, sequenced for their aforementioned genomic markers, and included in the phylogenetic analyses.

Description of Aphelenchoides hamospiculatus n. sp. (Aphelenchoidea: Aphelenchoididae) from Golestan province, north Iran

Nematology ◽  
2020 ◽  
pp. 1-13
Author(s):  
Parnaz Mortazavi ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Large Subunit ◽  
The Body ◽  
Lsu Rdna ◽  
Similar Species ◽  
Natural Forests ◽  
Rdna Sequences ◽  
Female Tail ◽  
Golestan Province ◽  
Type Population

Summary Two populations of Aphelenchoides hamospiculatus n. sp. were recovered from natural forests in eastern regions of Golestan province, north Iran. Both populations were morphologically similar. The new species is described and illustrated herein. The females of the type population, recovered from soil, measured 467-550 μm long, and females of the second population, from bark, measured 545-666 μm long. It is mainly characterised by having five lines in the lateral fields, a subcylindrical tail and a single centrally located mucron at the female tail tip with fine nodules, giving it a warty appearance. It is further characterised by having a cephalic region separated from the rest of the body by a shallow constriction, stylet with small swellings at base, large median bulb with slightly post-centrally located, well sclerotised valvular plates and post-vulval uterine sac 56-63 μm long in the type population. The males have spicules with a remarkable narrowing at the distal two-thirds, well-developed condylus with rounded end, small rostrum slightly bent inwards and a hook-like tip of the dorsal limb; and three pairs of cloacal + caudal papillae (the single precloacal papilla, P1, is lacking). The new species was morphologically compared with three species of the genus having five lines in the lateral fields, similar species with no data on their lateral lines, and those with similar spicules and a female tail end bearing a warty mucron. The molecular phylogenetic relationships of the new species using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences are discussed.

Morphological and molecular characterisation of Ektaphelenchoides tonekabonensis n. sp. (Rhabditida: Ektaphelenchinae) from northern Iran

Nematology ◽  
2019 ◽  
Vol 21 (10) ◽  
pp. 1011-1022 ◽  
Author(s):  
Farzad Aliramaji ◽  
Ebrahim Pourjam ◽  
Esmaeil Miraeiz ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Body Region ◽  
Lsu Rdna ◽  
Similar Species ◽  
Northern Iran ◽  
Body Contour ◽  
Phylogenetic Affinities

Summary Ektaphelenchoides tonekabonensis n. sp. is described and illustrated based upon morphological, morphometric and molecular characters. The new species is characterised by 476-659 μm long females, lip region broad and continuous with body contour, two weak lateral bands barely visible in lateral view and cross section, needle-like stylet with its conus forming ca 50% of the total length and with a very narrow lumen in the shaft, excretory pore located just opposite the metacorpus base or slightly posterior, a dense (sclerotised) mass just anterior to the vagina, a short post-uterine sac 5-7 μm long, elongate conoid distal body region ending in a long filiform part, males common with a dorsally convex conical tail ending to a filiform tip, 7-8 μm long spicules with a small projection at the capitulum close to the condylus, its tip posteriorly bent, and five precloacal + caudal papillae. Based upon morphological similarities and phylogenetic affinities, it is similar to six known species of the genus, namely: E. attenuata, E. caspiensis, E. fuchsi, E. kelardashtensis, E. musae, and E. ruehmi. Ektaphelenchoides caspiensis is typologically most similar to the new species and can be separated based on vulval position, excretory pore position and male tail characters. The next most similar species, E. kelardashtensis, has a longer stylet and posteriorly located excretory pore. Comparisons with the other aforementioned species and typologically similar species under Seinura are also discussed. Molecular phylogenetic analyses were performed using Bayesian inference (BI) based upon two partial small and large subunit ribosomal DNA (SSU and LSU rDNA D2-D3) sequences and the phylogenetic affinities of the new species in both phylogenies were discussed.

Ottolenchus sinipersici n. sp. (Rhabditida: Tylenchidae) from the Persian Gulf mangrove forests, Iran

Nematology ◽  
2021 ◽  
pp. 1-15
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Joaquín Abolafia ◽  
Akbar Karegar ◽  
Reza Ghaderi ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Phylogenetic Trees ◽  
Large Subunit ◽  
Small Subunit ◽  
The Body ◽  
Lsu Rdna ◽  
Mangrove Forests ◽  
Body Contour ◽  
Lateral Field

Summary A new species of Tylenchidae from the rhizosphere of mangrove trees in Hormozgan and Khuzestan provinces, Iran, is described based on morphological and molecular data. Ottolenchus sinipersici n. sp., is characterised by a slightly fusiform body 560-665 μm long, lateral field in the form of a narrow band with two faint incisures that are not visible in fatter females, indistinct transverse annuli under the light microscope, cephalic region continuous with the body contour, smooth and flattened dorsoventrally, longitudinal and narrow sigmoid amphidial slits, stylet delicate, 10.1-11.2 μm long, with small rounded to slightly posteriorly sloping knobs, well-developed median bulb, offset and pyriform pharyngeal basal bulb, vulva located at 66.9-69.6% of body length, offset spermatheca, short post-vulval uterine sac, spicules 18.5-20.5 μm long with highly curved blades, and a 113-135 μm long filiform tail with a hook-like or coiled terminus. In Bayesian inference phylogenetic trees based on the partial small subunit ribosomal DNA (SSU rDNA) and D2-D3 expansion segment of large subunit ribosomal DNA (D2-D3 LSU rDNA) genes, the new species clustered together with O. facultativus (KJ869310) in SSU, and forms a clade with three isolates of O. discrepans in LSU phylogeny. Ottolenchus fungivorus n. comb. (= Filenchus fungivorus) is proposed.

Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Molecular Characteristics ◽  
Lsu Rdna ◽  
Northern Iran ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 

New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran

Nematology ◽  
2019 ◽  
Vol 21 (9) ◽  
pp. 937-956 ◽  
Author(s):  
Yousef Panahandeh ◽  
Ebrahim Pourjam ◽  
Sergio Álvarez-Ortega ◽  
Farahnaz Jahanshahi Afshar ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Small Subunit ◽  
Lsu Rdna ◽  
Natural Forests ◽  
Electron Microscopic ◽  
Pharyngeal Bulb ◽  
Ribosomal Rna Gene ◽  
Molecular Approaches

Summary During nematological surveys in grasslands and natural forests of north and north-western Iran, three species of Miculenchus, including two new and one known species, were recovered and characterised based upon morphological and molecular approaches. Miculenchus brevisalvus n. sp., the first new species, is mainly characterised by its short females 334-388 μm long and with a short 6.0-7.5 μm long stylet, pyriform to pyriform-elongate pharyngeal bulb, 4-8 μm long post-uterine sac (PUS), offset rounded spermatheca filled with small spheroid sperm, elongate conoid tail 62-83 μm long with a sharp tip, and males with simple cloacal lips. Miculenchus muscus n. sp., the second new species, is characterised by a combination of the following features: body 401-467 μm long, well-developed protuberant labial plate at the anterior end under light microscopy, stylet 7-9 μm long, pyriform pharyngeal bulb, PUS 4-9 μm long, gradually narrowing conical tail 62-74 μm long with a finely pointed or sharp end and bearing several fine bristles at tip, and a male with projecting cloacal lips. Both newly described species were morphologically compared with four currently known species of the genus, viz., M. elegans, M. salmae, M. salvus, and M. tesselatus. Miculenchus salmae was also recovered and reported from Iran for the first time. It is mainly characterised by lacking a PUS and the characteristic vagina shape. Miculenchus muscus n. sp. and M. salmae were both characterised using scanning electron microscopic images, yielding new morphological observations for the genus. All three species are studied for their molecular phylogenetic characters using sequences of near-full length fragments of the small subunit ribosomal RNA gene (SSU rDNA) and the D2-D3 expansion segments of the large subunit ribosomal RNA gene (LSU rDNA D2-D3). In both SSU and LSU phylogenies, all currently sequenced species of Miculenchus formed a monophyletic group with maximal clade support in both Bayesian inference and maximum likelihood analysis.

Molecular and morphological characterisation of Aphelenchoides kheirii n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus nigra in north-western Iran

Nematology ◽  
2018 ◽  
Vol 20 (9) ◽  
pp. 877-888
Author(s):  
Behrouz Golhasan ◽  
Ramin Heydari ◽  
Mehrab Esmaeili ◽  
Natsumi Kanzaki
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Pinus Nigra ◽  
Small Subunit ◽  
Similar Species ◽  
Western Iran ◽  
Lateral Lines ◽  
Pine Trees ◽  
Group 2 ◽  
North Western

Summary Aphelenchoides kheirii n. sp. was isolated during a survey of nematodes associated with bark samples of pine trees (Pinus nigra) in West Azerbaijan province, north-western Iran. The new species has a body length of 500 (448-520) μm in the female and 442 (402-480) μm in the male. The cuticle is weakly annulated with four lateral lines and the lip region is offset. The stylet is 10.4 (10-11) μm long with small basal swellings and the excretory pore located immediately posterior to base of metacorpus; hemizonid invisible. The post-vulval uterine sac length 37.7 (28-45) μm. Spicules are relatively short (18-20 μm in dorsal limb) and the end of the dorsal limb clearly curved ventrally like a hook. The male tail has usual three pairs of caudal papillae (2 + 2 + 2) and a well-developed mucron. The female tail is conical, terminating in a complicated step-like projection, usually with many tiny nodular protuberances. The new species belongs to the Group 2 category of Aphelenchoides species sensu Shahina in which ten known species among Groups 2 and 4 sensu Shahina, namely: A. arcticus, A. blastophthorus, A. fuchsi, A. parasaprophilus, A. paraxui, A. xui, A. dactylocercus, A. gynotylurus, A. iranicus and A. saprophilus are the most similar species. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rRNA supported the morphological results.

A new entomopathogenic nematode, Steinernema hebeiense sp. n. (Rhabditida: Steinernematidae), from North China

Nematology ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 563-574 ◽  
Author(s):  
Maurice Moens ◽  
Sergei Spiridonov ◽  
Aihua Yan ◽  
Shulong Chen ◽  
Xiuhua Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Total Body Length ◽  
Molecular Data ◽  
Body Region ◽  
Lsu Rdna ◽  
Lateral Field ◽  
Total Body

AbstractA new species of Steinernema (Rhabditida), S. hebeiense sp. n. was isolated from sandy soil, Hebei Province, China. Diagnostic characters of infective juveniles of S. hebeiense sp. n. include total body length of 658 (610-710) μm, distance from anterior end to excretory pore of 48 (43-51) μm, tail length of 66 (63-71) μm, and E% ratio of 72 (65-80). The lateral field has eight ridges at the mid-body region (two very thin submarginal ridges, poorly discernible under light microscope, but visible in SEM); and the anterior end is slightly offset and flattened. First generation males have a body length of 1177 (1036-1450) μm, spicule length = 57 (51-63) μm, and gubernaculum length = 46 (38-50) μm. The spicules are light brown in colour and slightly curved; the manubrium is longer than wide. A tail mucron is present in second generation males. These morphometrical and morphometric features classify S. hebeiense sp. n. within the ' feltiae-kraussei-oregonense ' group. Molecular data also classify the new species within this group. Within this group, the smallest differences between the sequences of S. hebeiense sp. n. is with S. weiseri – 73 bp in the ITS rDNA and 13 bp in the studied partial sequence of the D2D3 expansion segment of LSU rDNA.

A new species of Basilaphelenchus Pedram, Kanzaki, Giblin-Davis & Pourjam, 2018 (Aphelenchoidea: Tylaphelenchinae), from natural forests of Golestan province, Iran

Nematology ◽  
2020 ◽  
Vol 22 (4) ◽  
pp. 361-371 ◽  
Author(s):  
Farzad Aliramaji ◽  
Zeinab Mirzaie Fouladvand ◽  
Ebrahim Pourjam ◽  
Parnaz Mortazavi ◽  
Farahnaz Jahanshahi Afshar ◽  
...  
Keyword(s):  
New Species ◽  
Body Region ◽  
Natural Forests ◽  
Rotten Wood ◽  
Golestan Province ◽  
The Common ◽  
Taxonomic Approach ◽  
Two Populations ◽  
A New Species ◽  
Small Valve

Summary Two populations of an aphelenchoidid nematode were recovered from natural forests in Golestan province, Iran. Both populations resembled each other morphologically. The females of the first population, from rotten wood of Fagus orientalis in Golestan National Park, measured 294-338 μm long, and females of the second population, from forests of Ramian county, measured 365-464 μm long. A reverse taxonomic approach, based upon SSU and LSU phylogenies using a maximal number of Aphelenchoididae genera, revealed the two isolates to be conspecific and representatives of a new species of Basilaphelenchus. Basilaphelenchus magnabulbus n. sp. is described and illustrated herein and is mainly characterised by the unique form of the metacorpus, this being elongate and filling the corresponding body region similar to the common form in other aphelenchoidids (vs smaller with well posteriorly located small valve in previously described species of the genus). The new species has an elevated lip region, fine stylet with three elongate posteriorly directed knobs, female tail elongate conoid and ventrally bent, and male tail short conical with three pairs of caudal papillae. It was morphologically compared with four previously known species of the genus, viz., B. persicus, B. brevicaudatus, B. gorganensis and B. grosmannae, and three species of Aphelenchoides having a broadly similar morphology.

Seinura caverna n. sp. (Tylenchomorpha: Aphelenchoididae), an androdioecious species isolated from bat guano in a calcareous cave

Nematology ◽  
2019 ◽  
Vol 21 (2) ◽  
pp. 207-225 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Taisuke Ekino ◽  
Hayato Masuya
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Reproductive Mode ◽  
Similar Species ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Rna Genes ◽  
A New Species ◽  
Basal Position ◽  
Bat Guano

Summary A new species of a predator aphelenchoidid, Seinura caverna n. sp., is described and illustrated. The new species was isolated from bat guano collected from a calcareous cave in Japan. The new species is characterised by its three-lined lateral field, secretory-excretory pore at the level of the posterior two-thirds of the metacorpus, a long post-uterine sac, hermaphrodite tail shape elongate conoid with a filiform tip, and an androdioecious reproductive mode. The new species is typologically and biologically close to S. steineri, but is distinguished by its longer post-uterine sac, slightly longer stylet and slightly larger median bulb. The comparisons with other morphologically similar species, i.e., S. chertkovi, S. tenuicaudata and S. steineri, are discussed. A molecular phylogenetic analysis based on two small and large subunit ribosomal RNA genes revealed that the new species is located at the basal position of clade 3 of the Aphelenchoididae, clearly separate from S. demani, its congener with unclear rectum and anus, which is located at the derived position, suggesting that further generic revision is necessary for the genus. Aphelenchoides lii n. comb. (= Seinura lii) is proposed.

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