Response of soybean genotypes from Northeast China to Heterodera glycines races 4 and 5, and characterisation of rhg1 and Rhg4 genes for soybean resistance

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Minghui Huang ◽  
Ruifeng Qin ◽  
Chunjie Li ◽  
Mingze Wang ◽  
Ye Jiang ◽  
...  
Keyword(s):  
Northeast China ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Pcr Amplification ◽  
Molecular Characteristics ◽  
Breeding Lines ◽  
Soybean Genotypes ◽  
The Usa ◽  
Local Breeding ◽  
Scn Resistance

Summary Soybean cyst nematode (SCN, Heterodera glycines) is a devastating plant-parasitic nematode worldwide. Two SCN races, race 4 (HG Type 1.2.3.5.6.7) and race 5 (HG Type 2.5.7), with increased virulence were previously identified in Northeast China. To obtain new resistance sources to these SCN populations, the response of 62 genotypes, including 51 local varieties and breeding lines, and 11 indicator lines for SCN race and HG Type identification, were evaluated. Four new primers in the regions of two loci of GmSHMT08 (Rhg4) and GmSNAP18 (rhg1) were designed for PCR amplification and subsequent sequencing to characterise haplotypes instead of genome resequencing. Results indicated three haplotypes among 51 local genotypes; there were 26 lines in Haplotype I carrying both the rhg1-a and Rhg4-a resistant loci as in ‘Peking’, 13 lines in Haplotype II containing only the resistant rhg1-a locus but Rhg4-b susceptible loci, and 12 lines in Haplotype III with rhg1-c and Rhg4-b susceptible loci. Interestingly, there was no ‘PI 88788’-type resistance identified in Northeast China, although it accounts for 90% of sources in the USA. Two local breeding lines in Haplotype I displayed resistance to both SCN races. The resistance lines carried higher copy number (>1) of the tandem duplication at the rhg1 locus compared with susceptible lines (⩽1). The combination of the two microsatellite markers, Sat_162 on Chr 8 and 590 on Chr 18, distinguished the three haplotypes and predicted the resistance/susceptibility for SCN race 5. The knowledge of the phenotypes and molecular characteristics of 51 local breeding lines in Northeast China will accelerate the utilisation of sources for broad-based SCN resistance and marker-assisted selection.

Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Ik-Young Choi ◽  
Prakash Basnet ◽  
Hana Yoo ◽  
Neha Samir Roy ◽  
Rahul Vasudeo Ramekar ◽  
...  
Keyword(s):  
Soybean Cyst Nematode ◽  
Gene Families ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Soybean Breeding ◽  
Resistant Varieties ◽  
Soybean Genotypes ◽  
Glycine Max L ◽  
Scn Resistance

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.

scholarly journals Soybean Stem Colonization by Genotypes A and B of Cadophora gregata Increases with Increasing Population Densities of Heterodera glycines

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1297-1301 ◽  
Author(s):  
G. M. Tabor ◽  
G. L. Tylka ◽  
C. R. Bronson
Keyword(s):  
Population Density ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Stem Rot ◽  
Population Densities ◽  
Brown Stem Rot ◽  
Phialophora Gregata ◽  
Soybean Genotypes ◽  
Genotype A

Growth chamber experiments were conducted to investigate whether parasitism by increasing population densities of Heterodera glycines, the soybean cyst nematode, increases the incidence and severity of stem colonization by the aggressive genotype A and the mild genotype B of Cadophora gregata (Phialophora gregata), causal agents of brown stem rot of soybeans. Soybean genotypes with three combinations of resistance and susceptibility to H. glycines and genotype A of C. gregata were inoculated with each genotype of C. gregata alone or each genotype with two population densities of H. glycines eggs, 1,500 or 10,000 per 100 cm3 of soil. Stems of two H. glycines-susceptible soybeans were more colonized by both aggressive and mild genotypes of C. gregata in the presence of high than in the presence of low H. glycines population density.

scholarly journals Genetic diversity in soybean genotypes with resistance to Heterodera glycines

2011 ◽  
Vol 11 (4) ◽  
pp. 304-312 ◽  
Author(s):  
Éder Matsuo ◽  
Tuneo Sediyama ◽  
Cosme Damião Cruz ◽  
Rosângela D'Arc de Lima Oliveira ◽  
Rita de Cássia Teixeira Oliveira ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Morphological Characteristics ◽  
Optimization Method ◽  
Genotypic Differences ◽  
Performance Tests ◽  
Breeding Programs ◽  
Soybean Genotypes ◽  
Superior Genotypes

The purpose of this study was to analyze the genetic diversity among soybean genotypes inoculated with Heterodera glycines race 3. The experiments were conducted in a greenhouse. In two performance tests of morphological characteristics and resistance to the pathogen, 27 soybean genotypes were assessed. The coefficient of genotypic determination was estimated by the method of analysis of variance and the genetic diversity analyzed based on dendrograms and optimization method. The estimated coefficients of determination indicated a predominantly genetic origin of the genotypic differences in the traits. The genetic variability was maintained in the superior genotypes, which can be used in breeding programs for resistance to soybean cyst nematode.

scholarly journals Heterodera glycines Infection Increases Incidence and Severity of Brown Stem Rot in Both Resistant and Susceptible Soybean

Plant Disease ◽  
2003 ◽  
Vol 87 (6) ◽  
pp. 655-661 ◽  
Author(s):  
G. M. Tabor ◽  
G. L. Tylka ◽  
J. E. Behm ◽  
C. R. Bronson
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Stem Rot ◽  
Growth Chamber ◽  
Susceptible Genotype ◽  
Brown Stem Rot ◽  
Soybean Cultivars ◽  
Phialophora Gregata ◽  
Soybean Genotypes

Growth chamber experiments were conducted to investigate whether parasitism by Heterodera glycines, the soybean cyst nematode, increases incidence and severity of brown stem rot (BSR) of soybean, caused by Phialophora gregata, in both resistant and susceptible soybean cultivars. Soybean genotypes with various combinations of resistance and susceptibility to both pathogens were inoculated with P. gregata alone or P. gregata plus H. glycines. In most tests of H. glycines-susceptible genotypes, incidence and severity of internal stem discoloration, characteristic of BSR, was greater in the presence than in the absence of H. glycines, regardless of susceptibility or resistance to BSR. There was less of an increasing effect of H. glycines on stem symptoms in genotypes resistant to both BSR and H. glycines; however, P. gregata colonization of these genotypes was increased. Stems of both a BSR-resistant and a BSR-susceptible genotype were colonized earlier by P. gregata in the presence than in the absence of H. glycines. Our findings indicate that H. glycines can increase the incidence and severity of BSR in soybean regardless of resistance or susceptibility to either pathogen.

Heterodera glycines (soybean cyst nematode).

2021 ◽  
Author(s):  
David Hunt
Keyword(s):  
Soviet Union ◽  
Former Soviet Union ◽  
Plant Material ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Growing Season ◽  
Cyst Nematode ◽  
The Usa ◽  
Major Pest ◽  
Semi Arid

Abstract The soybean cyst nematode H. glycines is a known major pest of soybean in regions of the USA particularly semi-arid areas. The nematode has now been found as a pest of soybean outside the USA in Argentina, Brazil, Colombia, China, Egypt, Indonesia, Iran, Italy, Japan, Korea, Paraguay and the former Soviet Union. Other hosts include Phaseolus beans. It can survive in a semi-dried state and is easily spread in soil or on plant material. It is a pest in temperate areas and does not develop below 15°C or above 33°C. Once introduced, the populations of the nematode can rapidly increase as it will complete 6-7 generations per growing season.

scholarly journals Soybean Resistance to the Soybean Cyst Nematode Heterodera glycines: An Update

2016 ◽  
Vol 106 (12) ◽  
pp. 1444-1450 ◽  
Author(s):  
Melissa G. Mitchum
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Natural Resistance ◽  
Biochemical Basis ◽  
The World ◽  
Scn Resistance ◽  
Soybean Resistance ◽  
Nematode Virulence

The soybean cyst nematode (SCN), Heterodera glycines, remains a serious threat to soybean production throughout the world. A lack of genetic diversity in resistant soybean cultivars has led to a widespread shift toward virulence in SCN populations, leaving farmers with few proven options other than nonhost rotation to manage this nematode. Recent advances in our understanding of the genes controlling resistance to the nematode have led to improved molecular markers, which are, in turn, increasing the efficiency and precision of the breeding pipeline. A better understanding of the molecular and biochemical basis of SCN resistance and nematode virulence will provide information useful for the development of a long-term strategic plan for diversification and the deployment of cultivars that protect current sources of natural resistance while identifying new targets for engineering novel resistance.

scholarly journals Effect of Soybean Cyst Nematode Resistance Source and Seed Treatment on Population Densities of Heterodera glycines, Sudden Death Syndrome, and Yield of Soybean

Plant Disease ◽  
2017 ◽  
Vol 101 (12) ◽  
pp. 2137-2143 ◽  
Author(s):  
Yuba R. Kandel ◽  
Kiersten A. Wise ◽  
Carl A. Bradley ◽  
Martin I. Chilvers ◽  
Adam M. Byrne ◽  
...  
Keyword(s):  
Sudden Death ◽  
Soybean Cyst Nematode ◽  
Seed Treatment ◽  
Heterodera Glycines ◽  
Plant Introduction ◽  
Cyst Nematode ◽  
Sudden Death Syndrome ◽  
Population Densities ◽  
Scn Resistance ◽  
Pi 88788

A three-year study was conducted in Illinois, Indiana, Iowa, Michigan, and Ontario, Canada, from 2013 through 2015 to determine the effect of soybean (Glycine max) cultivars’ source of soybean cyst nematode (SCN; Heterodera glycines) resistance on SCN population densities, sudden death syndrome (SDS; caused by Fusarium virguliforme), and yield of soybean. Five cultivars were evaluated with and without fluopyram seed treatment at each location. Cultivars with no SCN resistance had greater SDS severity, greater postharvest SCN egg counts (Pf), and lower yield than cultivars with plant introduction (PI) 548402 (Peking) and PI 88788-type of SCN resistance (P < 0.05). Cultivars with Peking-type resistance had lower Pf than those with PI 888788-type and no SCN resistance. In two locations with HG type 1.2-, cultivars with Peking-type resistance had greater foliar disease index (FDX) than cultivars with PI 88788-type. Fluopyram seed treatment reduced SDS and improved yield compared with a base seed treatment but did not affect SCN reproduction and Pf (P > 0.05). FDX and Pf were positively correlated in all three years (P < 0.01). Our results indicate that SDS severity may be influenced by SCN population density and HG type, which are important to consider when selecting cultivars for SCN management.

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