Kinetics of in Vitro Lactoferrin Deposition on Silicone Hydrogel and FDA Group II and Group IV Hydrogel Contact Lens Materials

ABSTRACT Aim The purpose of this study was to evaluate the fracture strength of roots instrumented with the self-adjusting file (SAF; ReDent-Nova, Ra'anana, Israel) and the Reciproc reciprocating file and that were and were not obturated using the warm vertical lateral compaction technique. Materials and methods In total, 75 mandibular premolar teeth were sectioned at or below the cementoenamel junction to obtain roots 13 mm in length. The roots were balanced with respect to buccolingual and mesiodistal diameters and weight. They were distributed into four experimental groups and one control group (n = 15): No instrumentation (group I), instrumentation with SAF files but no obturation (group II), instrumentation with SAF files and obturated with warm vertical lateral compaction (group III), instrumentation with Reciproc File but no obturation (group IV), and instrumentation with Reciproc File and obturated with warm vertical lateral compaction (group V). AH Plus sealer (Dentsply DeTrey, Konstanz, Germany) was used along with gutta-percha points. One week later, a vertical load was applied to the specimen's canal until fracture occurred. Data were statistically analyzed using one-way analysis of variance (p = 0.05). Results The mean fracture load was 312.83 N for group I, 297.35 N for group II, 359.15 N for group III, 231.51 N for group IV, and 275.81 N for group V. Conclusion The fracture resistances exhibited a statistically significant difference between all the groups. Teeth instrumented by SAF exhibited a better fracture resistance. How to cite this article Tyagi S, Choudhary E, Kabra P, Chauhan R. An in vitro comparative Evaluation of Fracture Strength of Roots Instrumentated with Self-adjusting File and Reciproc Reciprocating File, with and without Obturation. Int J Clin Dent Res 2017;1(1):20-25.

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Videokeratoscopic assessment of silicone hydrogel contact lens wettability using a new in-vitro method

Contact Lens and Anterior Eye ◽

10.1016/j.clae.2019.07.005 ◽

2019 ◽

Vol 42 (6) ◽

pp. 614-619 ◽

Cited By ~ 1

Author(s):

Erol Havuz ◽

Muveyla N. Gurkaynak

Keyword(s):

Contact Lens ◽

In Vitro Method ◽

Silicone Hydrogel

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Quantity and Conformation of Lysozyme Deposited on Conventional and Silicone Hydrogel Contact Lens Materials Using an In Vitro Model

Eye & Contact Lens Science & Clinical Practice ◽

10.1097/01.icl.0000244155.87409.f6 ◽

2007 ◽

Vol 33 (3) ◽

pp. 138-143 ◽

Cited By ~ 68

Author(s):

Maciej Suwala ◽

Mary-Ann Glasier ◽

Lakshman N. Subbaraman ◽

Lyndon Jones

Keyword(s):

Contact Lens ◽

In Vitro Model ◽

Silicone Hydrogel ◽

Vitro Model

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In vitro release of phospholipid from a silicone hydrogel contact lens

Contact Lens and Anterior Eye ◽

10.1016/s1367-0484(11)60077-5 ◽

2011 ◽

Vol 34 ◽

pp. S15 ◽

Cited By ~ 1

Author(s):

William G Pitt ◽

Yibei Zhao ◽

Daniel R Jack ◽

Jared L Nelson ◽

John D Pruitt

Keyword(s):

Contact Lens ◽

In Vitro Release ◽

Silicone Hydrogel ◽

Vitro Release

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In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials

Clinical Ophthalmology ◽

10.2147/opth.s270575 ◽

2020 ◽

Vol Volume 14 ◽

pp. 2821-2828

Author(s):

Han Qiao ◽

Doerte Luensmann ◽

Miriam Heynen ◽

Elizabeth Drolle ◽

Lakshman N Subbaraman ◽

...

Keyword(s):

Cholesteryl Ester ◽

Contact Lens ◽

In Vitro Evaluation ◽

Silicone Hydrogel

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Parasympathomimetic Effect of Shilajit Accounts for Relaxation of Rat Corpus Cavernosum

American Journal of Men s Health ◽

10.1177/1557988312462738 ◽

2012 ◽

Vol 7 (2) ◽

pp. 119-127 ◽

Cited By ~ 5

Author(s):

Sarabjeet Kaur ◽

Pravin Kumar ◽

Deo Kumar ◽

M. D. Kharya ◽

Nityanand Singh

Keyword(s):

Corpus Cavernosum ◽

Saline Group ◽

Group Iv ◽

In Vivo Study ◽

Group Iii ◽

Group I ◽

Group Ii ◽

Vitro Effect

Previous studies have reported an enhancement of central cholinergic signal cascade by shilajit. For the present study, it was hypothesized that parasympathomimetic effect of shilajit accounting for relaxation of rat corpus cavernosum may be one of the major mechanisms attributing to its traditional role as an aphrodisiac. To test this hypothesis, the acute peripheral effect of standard acetylcholine (ACh), shilajit, and their combination was evaluated on cardiorespiratory parameters such as mean arterial blood pressure (MABP), heart rate (HR), respiratory rate (RR), and neuromuscular transmission (NMT). Furthermore, in vitro effect of standard ACh, shilajit, and their combination was tested on the rat corpus cavernosum. Six groups were used for the in vivo study ( N = 5): Group I (control-saline), Group II (ACh), Group III (Sh), Group IV (Sh followed by ACh), Group V (Atropine followed by ACh), and Group VI (Atropine followed by Sh). The in vitro study included four groups: Group I (control-saline), Group II (ACh), Group III (Sh), and Group IV (Sh followed by ACh). The results of the in vivo study confirmed the peripheral parasympathomimetic effect of shilajit (400 µg/mL). The in vitro results revealed that shilajit (400 and 800 µg/mL) relaxed cavernous strips’ concentration dependently and enhanced ACh-mediated relaxations. The peripheral parasympathomimetic effects of shilajit were confirmed by blockade of shilajit-induced relaxations (in vitro) and shilajit-induced lowering of MABP and HR (in vivo) by atropine.

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Enhancement of in Vitro Developmental Outcome of Cloned Goat Embryos After Epigenetic Modulation of Somatic Cell-Inherited Nuclear Genome with Trichostatin A

Annals of Animal Science ◽

10.2478/aoas-2019-0063 ◽

2020 ◽

Vol 20 (1) ◽

pp. 97-108

Author(s):

Maria Skrzyszowska ◽

Marcin Samiec

Keyword(s):

Somatic Cell ◽

Trichostatin A ◽

Nuclear Genome ◽

Donor Cell ◽

Group Iv ◽

Control Group ◽

Group I ◽

Donor Cells ◽

Group Ii

AbstractIn this study, the effect of trichostatin A (TSA)-mediated epigenomic modulation of nuclear donor cells on the in vitro developmental potential of caprine somatic cell cloned embryos was examined. The enucleated ex vivo-matured oocytes were subzonally injected with adult ear skin-derived fibroblast cells exposed or not exposed to TSA (at a concentration of 50 nM). The experiment was designed on the basis of three different approaches to TSA-dependent modulation of donor cell-descended genome: before being used for somatic cell nuclear transfer/SCNT (Group I); immediately after activation of nuclear-transferred (NT) oocytes (Group II); or combined treatment both before being used for SCNT and after activation of NT oocytes (Group III). In the control Group IV, donor cell nuclei have not been treated with TSA at any stage of the experimental design. In TSA-treated Groups I and II and untreated Group IV, cleavage activities of cloned embryos were at the similar levels (80.6%, 79.8% and 77.1%, respectively). But, significant difference was observed between Groups III and IV (85.3 vs. 77.1%). Moreover, in the experimental Groups I and III, the percentages of cloned embryos that reached the blastocyst stages remarkably increased as compared to those noticed in the control Group IV (31.2% vs. 36.7% vs. 18.9%, respectively). In turn, among embryos assigned to Group II, blastocyst formation rate was only slightly higher than that in the control Group IV, but the differences were not statistically significant (25.8% vs. 18.9%). To sum up, TSA-based epigenomic modulation of somatic cell-inherited nuclear genome gave rise to increased competences of caprine cloned embryos to complete their development to blastocyst stages. In particular, sequential TSA-mediated modulation of both nuclear donor cells and activated NT oocytes led to improvement in the blastocyst yields of cloned goat embryos, which can result from enhanced donor cell nuclear reprogrammability.

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