Histochemical and Immunocytochemical Identification of Alveolar Type II Epithelial Cells Isolated from Fetal Rat Lung

1988 ◽  
Vol 137 (3) ◽  
pp. 525-530 ◽  
Author(s):  
Martin Post ◽  
Barry T. Smith
Keyword(s):  
Epithelial Cells ◽  
Alveolar Type ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

Isolation of alveolar type II cells from fetal rat lung by differential adherence in monolayer culture

1988 ◽  
Vol 960 (3) ◽  
pp. 441-453 ◽  
Author(s):  
Joseph J. Batenburg ◽  
Caroline J.M. Otto-Verberne ◽  
Ank A.W. Ten Have-Opbroek ◽  
Wies Klazinga
Keyword(s):  
Monolayer Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat ◽  
Fetal Rat Lung

Growth of distal fetal rat lung epithelial cells in a defined serum-free medium

1991 ◽  
Vol 27 (8) ◽  
pp. 625-632 ◽  
Author(s):  
D. Jassal ◽  
R. N. N. Han ◽  
I. Caniggia ◽  
M. Post ◽  
A. K. Tanswell
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Rat Lung ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Fetal Rat Lung

scholarly journals Maternal administration of dexamethasone stimulates choline-phosphate cytidylyltransferase in fetal type II cells

1987 ◽  
Vol 241 (1) ◽  
pp. 291-296 ◽  
Author(s):  
M Post
Keyword(s):  
Enzyme Activity ◽  
Specific Activity ◽  
Active Form ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Dexamethasone Treatment ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Choline Phosphate

Administration of dexamethasone to pregnant rats at 19 days gestation increased phosphatidylcholine synthesis (45%) from radioactive choline in type II cells. This enhanced synthesis of phosphatidylcholine was accompanied by an increased conversion of choline phosphate into CDP-choline. Similar results were obtained by incubating organotypic cultures of 19-day-fetal rat lung with cortisol. The increased conversion of choline phosphate into CDP-choline correlated with an enhanced choline-phosphate cytidylyltransferase activity (31% after dexamethasone treatment; 47% after cortisol exposure) in the cell homogenates. A similar increase (26% after dexamethasone treatment; 39% after cortisol exposure) was found in the microsomal-associated enzyme. No differences in cytosolic enzyme activity were observed. The specific activity of the microsomal enzyme was 3-4 times that of the cytosolic enzyme. Most of the enzyme activity was located in the microsomal fraction (58-65%). The treatments had no effect on the total amount of enzyme recovered from the cell homogenates. These results, taken collectively, are interpreted to indicate that the active form of cytidylyltransferase in type II cells is the membrane-bound enzyme and that cytidylyltransferase activation in type II cells from fetal rat lung after maternal glucocorticoid administration occurs by binding of inactive cytosolic enzyme to endoplasmic reticulum.

scholarly journals Cigarette Smoke Induces Apoptosis by Activation of Caspase-3 in Isolated Fetal Rat Lung Type II Alveolar Ep-ithelial Cells <i>in Vitro</i>

2013 ◽  
Vol 03 (01) ◽  
pp. 4-12 ◽  
Author(s):  
Asra Ahmed ◽  
James A. Thliveris ◽  
Anthony Shaw ◽  
Michael Sowa ◽  
James Gilchrist ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Caspase 3 ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung
Sign in / Sign up

Export Citation Format

Share Document