A new Crocus L. (Iridaceae) species from SE Turkey, based on morphological and molecular data

Phytotaxa ◽  
2015 ◽  
Vol 239 (3) ◽  
pp. 223 ◽  
Author(s):  
Osman Erol ◽  
Doerte Harpke ◽  
Hasan Yıldırım
Keyword(s):  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Morphological Characters ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Phylogenetic Position ◽  
Full Description ◽  
New Taxon ◽  
Internal Transcribed Spacer Region ◽  
Se Turkey

Crocus musagecitii is described as a new species. Diagnostic morphological characters, a full description and detailed illustrations are provided on the basis of the type specimen and wild specimens. Morphologically, C. musagecitii is close to Crocus biflorus subsp. pseudonubigena. Crocus musagecitii differs from C. biflorus subsp. pseudonubigena by the lack of stripes or narrow purplish tongue on outside of outer tepals, wider tepals, and homogenously yellow anthers. In order to clarify the phylogenetic position of this species within the Crocus adamii species complex, we sequenced the internal transcribed spacer region (ITS: ITS1 + 5.8SrDNA + ITS2) and 5’ external transcribed spacer (ETS) of the nuclear ribosomal DNA (rDNA). A phylogenetic tree obtained by Bayesian phylogenetic inference is given. Phylogenetic analyses revealed that the new taxon is close to C. munzurensis. Crocus musagecitii differs from its phylogenetically closest relative C. munzurensis by the corm tunics (C. musagecitii: coriaceus; C. munzurensis: membranous), the number of leaves (C. musagecitii: up to 8; C. munzurensis: up to 4) and non-hairy leaf margins.

scholarly journals Phylogenetic relationships and taxonomic position of genus Hyperacrius (Rodentia: Arvicolinae) from Kashmir based on evidences from analysis of mitochondrial genome and study of skull morphology

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10364
Author(s):  
Natalia I. Abramson ◽  
Fedor N. Golenishchev ◽  
Semen Yu. Bodrov ◽  
Olga V. Bondareva ◽  
Evgeny A. Genelt-Yanovskiy ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
De Novo ◽  
Phylogenetic Analyses ◽  
Complete Mitochondrial Genome ◽  
Morphological Characters ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Skull Morphology ◽  
Protein Coding ◽  
Protein Coding Genes

In this article, we present the nearly complete mitochondrial genome of the Subalpine Kashmir vole Hyperacrius fertilis (Arvicolinae, Cricetidae, Rodentia), assembled using data from Illumina next-generation sequencing (NGS) of the DNA from a century-old museum specimen. De novo assembly consisted of 16,341 bp and included all mitogenome protein-coding genes as well as 12S and 16S RNAs, tRNAs and D-loop. Using the alignment of protein-coding genes of 14 previously published Arvicolini tribe mitogenomes, seven Clethrionomyini mitogenomes, and also Ondatra and Dicrostonyx outgroups, we conducted phylogenetic reconstructions based on a dataset of 13 protein-coding genes (PCGs) under maximum likelihood and Bayesian inference. Phylogenetic analyses robustly supported the phylogenetic position of this species within the tribe Arvicolini. Among the Arvicolini, Hyperacrius represents one of the early-diverged lineages. This result of phylogenetic analysis altered the conventional view on phylogenetic relatedness between Hyperacrius and Alticola and prompted the revision of morphological characters underlying the former assumption. Morphological analysis performed here confirmed molecular data and provided additional evidence for taxonomic replacement of the genus Hyperacrius from the tribe Clethrionomyini to the tribe Arvicolini.

scholarly journals Campanula teutana, a new isophyllous Campanula (Campanulaceae) from the Adriatic region

Phytotaxa ◽  
2014 ◽  
Vol 162 (1) ◽  
pp. 1 ◽  
Author(s):  
Sandro Bogdanović ◽  
Salvatore Brullo ◽  
Ivana Rešetnik ◽  
Zlatko Satovic ◽  
Zlatko Liber
Keyword(s):  
Balkan Peninsula ◽  
Comparative Morphology ◽  
Distinct Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Sem Analysis ◽  
Phylogenetic Position ◽  
New Taxon ◽  
Floral Characters ◽  
Adriatic Region

The Balkan Peninsula and the amphi-Adriatic region are biodiversity centres with a high number of Campanula species, some of them with restricted and isolated distributions. Among those is the morphologically, karyologically and genetically well-defined linage of isophyllous Campanula species of the garganica clade, taxonomically treated as Campanula ser. Garganicae. In the central Adriatic, on the Island of Vis (Croatia), an isophyllous Campanula was found growing in rocky crevices of calcareous sea cliffs among chasmophytic vegetation with extremely challenging ecological conditions. Based on morphological characters (monopodial growth form, cordate to ovate basal leaves, campanulate corolla, presence of obtuse hairs at the base of filaments, capsule opening by basal pores and brown shiny seeds) the unidentified plant from the Island of Vis falls into ser. Garganicae. To establish the phylogenetic position and relationships of the unknown Campanula among other species of the garganica clade, nuclear ITS and chloroplast trnL-F sequences as well as comparative morphology, karyology and scanning electron microscopy of seeds were used. Due to its campanulate corolla the plants from Vis are morphologically close to C. portenschlagiana from the eastern Adriatic, from which they differ in several quantitative floral characters. The karyological analysis revealed a diploid chromosome number (2n = 34), while SEM analysis showed that seed coat of new taxon has weakly striate testa, thin wavy striped radial walls and slightly marked lumen. Additionally, molecular data show that the Vis taxon is clearly separated as a distinct species from other species of ser. Garganicae. Therefore, it is described and illustrated as a new species, Campanula teutana Bogdanović & Brullo.  Because of the small number of individuals within the single population, C. teutana should be included on the Red List of the vascular flora of Croatia as an endangered species (EN).

Affinities of four freshwater putative “Chantransia” stages (Rhodophyta) in Southern China from molecular and morphological data

Phytotaxa ◽  
2020 ◽  
Vol 441 (1) ◽  
pp. 47-59
Author(s):  
JIN-FEN HAN ◽  
FANG-RU NAN ◽  
JIA FENG ◽  
JUN-PING LV ◽  
QI LIU ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Sequence Data ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
Morphological Description

Four putative “Chantransia” isolates were collected from four locations in Hubei and Yunnan Provinces, China. Morphological analyses were conducted on all isolates. Two specimens (HB26 and YN2) fit the morphological description of A. pygmaea, while the other two isolates (YN1 and YN3) varied in morphology, but were within the circumscription of Audouinella hermannii. Due to the fact that the morphological characters of the “Chantransia” stages of order Batrachospermales and the species of genus Audouinella are too similar to be distinguished, a molecular analysis was performed to clarify the phylogenetic position of these four isolates based on rbcL and psbA sequences. Two “pygmaea” specimens collected from Jiugong Mountain, Hubei Province (HB26) and Shimen Gorge, Yunnan Province (YN2), such as S. jiugongshanensis and S. shimenxiaensis, are proposed primarily based on the DNA sequence data generated in this study. The description of these two new species provides more molecular data for phylogenetic analysis of the genus Sheathia. In addition to these newly described species, the results strongly support that those “hermannii” isolates (YN1 and YN3) collected from Yunnan Province were the “Chantransia” of S. arcuata. However, their gametophyte stages have not been found, meaning that critical diagnostic morphological features were unavailable and molecular methods were the only means for ascertaining their phylogenetic position. Considering the extensive application of the rbcL and psbA genes in phylogenetic analyses of freshwater red algae, we recommend using these two genes to identify species when no morphological characteristics are available.

Allium schisticola, a new species with peculiar morphological characters and an unexpected phylogenetic position

Phytotaxa ◽  
2020 ◽  
Vol 450 (3) ◽  
pp. 246-256
Author(s):  
ALIREZA DOLATYARI ◽  
HAMID MOAZZENI ◽  
SAEIDE HOSSEINI ◽  
FRANK R. BLATTNER ◽  
REINHARD M. FRITSCH
Keyword(s):  
New Species ◽  
Flower Color ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
Phylogenetic Position ◽  
Morphological Description ◽  
New Taxon ◽  
Meiotic Chromosomes ◽  
Relationship Of ◽  
A New Species

Allium schisticola is described from West Azarbaijan (Iran) as a new species. It is closely related to A. sabalense and A. sahandicum in having a similar flower color but differs by leaf, filament, and tepal characters. We investigated the phylogenetic relationship of the new species based on sequences of the chloroplast trnL-trnF and nuclear ribosomal DNA internal transcribed spacer (ITS) regions in A. subg. Melanocrommyum compared with 109 accessions of this subgenus. Our results confirm its placement in A. subg. Melanocrommyum. However, the ITS tree showed that the new species should belong to A. sect. Melanocrommyum despite the fact that it represents many morphological characters of A. sect. Acanthoprason. Most peculiar for the new species are obtuse tepals broadest near the tip (vs. very narrowly lanceolate up to triangular tepals in A. sect. Acanthoprason), with adaxially inconspicuous median vein (vs. conspicuous median vein in sect. Acanthoprason). The new species is diploid with a chromosome number of 2n = 2x = 16. Karyotype features and meiotic chromosomes behavior are presented for the new taxon. A detailed morphological description, illustrations, and a distribution map of the new species are given.

scholarly journals A new terrestrial species of Chiropterotriton (Caudata: Plethodontidae) from central Mexico

Zootaxa ◽  
2017 ◽  
Vol 4363 (4) ◽  
pp. 489 ◽  
Author(s):  
MIRNA G. GARCÍA-CASTILLO ◽  
SEAN M. ROVITO ◽  
DAVID B. WAKE ◽  
GABRIELA PARRA-OLEA
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Molecular Data ◽  
New Taxon ◽  
Ecological Diversity ◽  
Undescribed Species ◽  
Morphometric Data ◽  
One New Species ◽  
Small Genus

Chiropterotriton is a relatively small genus that comprises 15 species with great morphological and ecological diversity. In previous studies, molecular data provided evidence for a considerable number of species that remain undescribed. In this study, we describe one new species, Chiropterotriton chico sp. nov. based on molecular and morphological characters. We present mtDNA phylogenetic analyses using Bayesian inference and maximum likelihood that include all described and several undescribed species. Morphometric data from eight recognized species provide evidence for the distinctiveness of the new taxon. Description of this new species adds to the already high salamander diversity of the state of Hidalgo, which is an important area for the diversification of the genus. 

Crocus heilbronniorum, a new Turkish species of Series Lyciotauri (Iridaceae)

Phytotaxa ◽  
2017 ◽  
Vol 298 (2) ◽  
pp. 173
Author(s):  
OSMAN EROL ◽  
DOERTE HARPKE ◽  
ALMILA ÇİFTÇİ
Keyword(s):  
New Species ◽  
Chromosome Number ◽  
Its Region ◽  
Type Specimen ◽  
Morphological Characters ◽  
Phylogenetic Position ◽  
Full Description ◽  
Sw Turkey ◽  
A New Species ◽  
Half Length

Crocus heilbronniorum is described as a new species from Fethiye, Muğla province (SW Turkey). Diagnostic morphological characters, a full description and detailed illustrations are provided based on the type specimen and wild specimens. Morphologically, C. heilbronniorum is close to Crocus atrospermus, but the newly described species differs by its indistinct corm tunic neck, the presence of teeth on rings, basal leaf number and narrower leaves, star-like flowers, longer filaments, almost half length shorter anthers, and style always longer than stamens. Furthermore, Crocus heilbronniorum possesses a different chromosome number (2n = 2x = 12) with regard to C. atrospermus (2n = 10). In order to clarify the phylogenetic position of this species within Series Lyciotauri, data on ITS region sequences are also reported.

Five new morphological types of virgulate and microcotylous xiphidiocercariae based on morphological and molecular phylogenetic analyses

2019 ◽  
Vol 94 ◽  
Author(s):  
S.V. Shchenkov ◽  
S.A. Denisova ◽  
G.A. Kremnev ◽  
A.A. Dobrovolskij
Keyword(s):  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
The Family ◽  
Family Level ◽  
Molecular Phylogenetic ◽  
Morphological And Molecular Data ◽  
First Time

Abstract The phylogenetic position of most xiphidiocercariae from subgroups Cercariae virgulae and Cercariae microcotylae remains unknown or unclear, even at the family level. In this paper, we studied the morphology and molecular phylogeny of 15 microcotylous and virgulate cercariae (11 new and four previously described ones). Based on morphological and molecular data, we suggested five distinct morphological types of xiphidiocercariae, which are a practical alternative to Cercariae virgulae and Cercariae microcotylae subgroups. Four of these types correspond to actual digenean taxa (Microphallidae, Lecithodendriidae, Pleurogenidae and Prosthogonimidae), while the fifth is represented by Cercaria nigrospora Wergun, 1957, which we classified on the basis of molecular data for the first time. We reassessed the relative importance of morphological characters used for the classification of virgulate and microcotylous cercariae, and discussed the main evolutionary trends within xiphidiocercariae. Now stylet cercariae can be reliably placed into several sub-taxa of Microphalloidea on the basis of their morphological features.

scholarly journals Appearances are deceptive: molecular phylogeny recovers the Scaly Gecko Hemidactylus scabriceps (Reptilia: Squamata: Gekkonidae) as a member of a scansorial and rupicolous clade

2018 ◽  
Vol 10 (9) ◽  
pp. 12147
Author(s):  
Achyuthan N. Srikanthan ◽  
Gandla Chethan Kumar ◽  
Aishwarya J. Urs ◽  
Sumaithangi Rajagopalan Ganesh
Keyword(s):  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Molecular Data ◽  
Habitat Associations ◽  
Phylogenetic Position ◽  
Peninsular India ◽  
Rock Formations ◽  
Clade B ◽  
Voucher Specimens ◽  
Genetic Alliance

We reassess the systematics of Hemidactylus scabriceps, a recently rediscovered and poorly known gecko, and elucidate its phylogenetic position using molecular data for the first time.  Contrary to previous speculations prompted by its morphological resemblance to other terrestrial Hemidactylus, our phylogenetic analyses recovered H. scabriceps to be a part of a clade consisting of the large-bodied, rock-dwelling Hemidactylus – the H. prashadi group.  Hemidactylus scabriceps also shows high levels of intraspecific genetic divergence, indicative of cryptic diversity.  We also confirm the synonymy of the monotypic genus Lophopholis (erected for H. scabriceps) with Hemidactylus.  We elaborate on the morphology of the type specimen and other recent voucher specimens, and compare it with sister species and other ground-dwelling Hemidactylus in peninsular India.  Species distribution of this ‘outlier’ clade member has been modeled using MaxEnt.  These exercises confirm that it is primarily a smooth-scaled, plain-dwelling, terrestrial species unlike other members in its clade.  This unexpected pattern of genetic alliance and contrasting body form plus habitat associations further underscores the unstudied complexity of peninsular India’s geological history.  Historical denudation of rock formations could have driven evolution of some of these otherwise rupicolous, scansorial gekkonids into smaller terrestrial lizards.  

Revisit of European-Asiatic connections in Tayloria rudolphiana (Splachnaceae, Bryophyta) based on molecular data and new morphological evidence

Phytotaxa ◽  
2020 ◽  
Vol 438 (4) ◽  
pp. 247-255
Author(s):  
YAN-JUN YI ◽  
XIAO-XUAN XIAO ◽  
XIAO-XIA XU ◽  
SI HE
Keyword(s):  
Yunnan Province ◽  
Phylogenetic Analyses ◽  
Molecular Study ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Features ◽  
Central China ◽  
Morphological Evidence ◽  
Full Description ◽  
Photographic Images

A molecular study of a rare moss, Tayloria rudolphiana has revealed a first new occurrence of this species in Central China after its first collecting more than 100 years ago from southwestern Yunnan Province. The phylogenetic analyses based on rps4 and trnL-F sequences along with its morphological features affirm the taxonomic identity of this new population, representing the latest evidence of European-Asiatic connections of this species. Photographic images detailing key morphological characters and a full description of the species are provided. The taxonomic problems of this species within China are discussed.

scholarly journals The Complete DNA Sequence of the Mitochondrial Genome of a “Living Fossil,” the Coelacanth (Latimeria chalumnae)

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 995-1010 ◽  
Author(s):  
Rafael Zardoya ◽  
Axel Meyer
Keyword(s):  
Mitochondrial Genome ◽  
Tandem Repeats ◽  
Phylogenetic Analyses ◽  
Large Data ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Data Set ◽  
Living Fossil ◽  
Latimeria Chalumnae ◽  
Relationship Of

The complete nucleotide sequence of the 16,407-bp mitochondrial genome of the coelacanth (Latimeria chalumnae) was determined. The coelacanth mitochondrial genome order is identical to the consensus vertebrate gene order which is also found in all ray-finned fishes, the lungfish, and most tetrapods. Base composition and codon usage also conform to typical vertebrate patterns. The entire mitochondrial genome was PCR-amplified with 24 sets of primers that are expected to amplify homologous regions in other related vertebrate species. Analyses of the control region of the coelacanth mitochondrial genome revealed the existence of four 22-bp tandem repeats close to its 3′ end. The phylogenetic analyses of a large data set combining genes coding for rRNAs, tRNA, and proteins (16,140 characters) confirmed the phylogenetic position of the coelacanth as a lobe-finned fish; it is more closely related to tetrapods than to ray-finned fishes. However, different phylogenetic methods applied to this largest available molecular data set were unable to resolve unambiguously the relationship of the coelacanth to the two other groups of extant lobe-finned fishes, the lungfishes and the tetrapods. Maximum parsimony favored a lungfish/coelacanth or a lungfish/tetrapod sistergroup relationship depending on which transversion:transition weighting is assumed. Neighbor-joining and maximum likelihood supported a lungfish/tetrapod sistergroup relationship.

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