Reassessment of the species Stigeoclonium polyrhizum (Chaetophoraceae, Chaetophorales) based on morphological and molecular data

Four specimens of Stigeoclonium spp., sampled in China between 2015 and 2016, were identified as the species Stigeoclonium polyrhizum (Chaetophoraceae, Chaetophorales) due to their unique morphology. A large part of the main filament and branches was tightly enclosed by numerous rhizoidal branches, which extended downward to the base of the plant to form an expanded holdfast. S. polyrhizum has previously been regarded as a synonym of S. longipilum or other related species by most phycologists. Therefore, a reassessment of S. polyrhizum based on morphological and molecular data was conducted. Rhizoidal branch development of S. polyrhizum was also described, showing the abundant rhizoidal branches present from the juvenile stage to the mature stage under controlled culture conditions. Phylogenetic evidence, using nuclear-encoded SSU rDNA data, clearly revealed that the Chaetophoraceae diverged into two well-supported sister clades: the Chaetophora-clade and the Fritschiella-clade. S. polyrhizum was included in the Fritschiella-clade instead of the Chaetophora-clade where Chaetophora draparnaldioides (S. longipilum) was found. The morphological and molecular data unambiguously show that S. polyrhizum is a valid species rather than a synonym of S. longipilum or other related species. Thylakoid bands appressed to the periphery of the pyrenoid matrix of S. polyrhizum was also described.

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Morphological and molecular diversity in the freshwater crab Potamon ruttneri-P. gedrosianum species complex (Decapoda, Brachyura) indicate the need for taxonomic revision

Crustaceana ◽

10.1163/15685403-00003512 ◽

2016 ◽

Vol 89 (2) ◽

pp. 129-139 ◽

Cited By ~ 2

Author(s):

Alireza Keikhosravi ◽

Reza Naderloo ◽

Christoph D. Schubart

Keyword(s):

New Record ◽

Molecular Diversity ◽

Taxonomic Status ◽

Taxonomic Revision ◽

Molecular Data ◽

Valid Species ◽

Indus River ◽

Mitochondrial 16S Rdna ◽

Morphological And Molecular Data ◽

Incomplete Sampling

Many species and subspecies of Potamon have been described from the easternmost distribution of the genus in the western tributaries of the Indus River. Most of them were synonymised subsequently under the two names of currently valid species known from the region: Potamon gedrosianum Alcock, 1909 and Potamon ruttneri Pretzmann, 1962. Genetic and morphological information, based on mitochondrial 16S rDNA and the first male gonopod (G1), were gathered in the course of the present study. The corresponding results suggest the occurrence of four groups and question the taxonomic status of both species. We also revise the distribution range of both species, in particular that of P. gedrosianum, with a new record from Iran. Overall, the study reveals the need for a major revision using further morphological and molecular data. Because of the complexity of this necessary revision and the incomplete sampling, we here refrain from proposing any taxonomic conclusions.

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Experimental taxonomy exposes ontogenetic variability and elucidates the taxonomic value of claw configuration in MilnesiumDoyère, 1840 (Tardigrada: Eutardigrada: Apochela)

Contributions to Zoology ◽

10.1163/18759866-08502003 ◽

2016 ◽

Vol 85 (2) ◽

pp. 173-200 ◽

Cited By ~ 40

Author(s):

Witold Morek ◽

Piotr Gąsiorek ◽

Daniel Stec ◽

Brian Blagden ◽

Łukasz Michalczyk

Keyword(s):

Nuclear Dna ◽

Mitochondrial Gene ◽

Molecular Data ◽

Valid Species ◽

Species Differentiation ◽

28S Rrna ◽

Configuration Change ◽

Gene Coi ◽

Diagnostic Key ◽

Morphological And Molecular Data

In this paper we describe a new apochelan species, Milnesium variefidumsp. nov. from Scotland and provide novel morphological and molecular data for Milnesium berladnicorumCiobanu et al., 2014. The new species differs from the most similar M. berladnicorumby the presence of developmental dimorphism in claw configuration, absent or weakly developed cuticular bars under claws I-III, a different arrangement of cuticular pseudoplates, and by differences in the sequences of three nuclear DNA fragments: 18S rRNA (p-distance: 0.6%), 28S rRNA (2.0%), ITS-2 (9.3%), and on mitochondrial gene COI (12.4%). Although ontogenetic claw configuration change was suspected to occur in some Milnesiumspecies, we are the first to document it through the combined use of traditional, molecular and experimental methodologies. We discuss the implications of the observed phenomenon for the taxonomy of the genus and propose a new diagnostic key to all Milnesiumspecies described up to the end of 2015. We also review other traits used for species differentiation in the genus and offer recommendations to improve the quality of future descriptions as well as suggest a need for integrative redescriptions of the known species. Finally, we propose to suppress M. dujiangensisand M. tardigradum trispinosumand suggest that M. alpigenumand M. quadrifidumare valid species that require thorough redescriptions.

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Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the “Crangonyx floridanus complex”

Zootaxa ◽

10.11646/zootaxa.4691.4.1 ◽

2019 ◽

Vol 4691 (4) ◽

pp. 301-332 ◽

Cited By ~ 1

Author(s):

ANDREW G. CANNIZZARO ◽

THOMAS R. SAWICKI

Keyword(s):

New Species ◽

Related Species ◽

Species Delimitation ◽

Species Complex ◽

Morphological Characteristics ◽

Molecular Data ◽

Closely Related Species ◽

Leon County ◽

Two New Species ◽

Morphological And Molecular Data

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses.

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“Ino Budensis var. Mollis” Grum-Grshimailo, 1893 (Lepidoptera: Zygaenidae) from Eastern Asia recognized as a valid species on the base of morphological and molecular analysis

Zootaxa ◽

10.11646/zootaxa.4619.3.5 ◽

2019 ◽

Vol 4619 (3) ◽

pp. 518-526

Author(s):

KONSTANTIN A. EFETOV ◽

GERHARD M. TARMANN ◽

EKATERINA V. PARSHKOVA

Keyword(s):

Molecular Analysis ◽

Molecular Data ◽

Identification Key ◽

Valid Species ◽

Eastern Asia ◽

Morphological And Molecular Data

Based on morphological and molecular data, Ino budensis var. mollis Grum-Grshimailo, 1893, from China, so far treated as a synonym of Jordanita (Roccia) paupera (Christoph, 1887), is here recognized as a good species, Jordanita (Roccia) mollis (Grum-Grshimailo, 1893), stat. nov. This species is recorded as new for the fauna of Russia and Korea. An identification key for this species is provided.

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A revision of the Trimeresurus puniceus-complex (Serpentes: Viperidae: Crotalinae) based on morphological and molecular data

Zootaxa ◽

10.11646/zootaxa.1293.1.1 ◽

2006 ◽

Vol 1293 (1) ◽

pp. 1 ◽

Cited By ~ 2

Author(s):

PATRICK DAVID ◽

GERNOT VOGEL ◽

S. P. VIJAYAKUMAR ◽

NICOLAS VIDAL

Keyword(s):

Species Concept ◽

Distinct Species ◽

Morphological Characters ◽

Molecular Data ◽

Biological Species ◽

Valid Species ◽

Biological Species Concept ◽

Phylogenetic Species Concept ◽

Morphological And Molecular Data ◽

A New Species

The brown Asian pitvipers of the genus Trimeresurus related to Trimeresurus puniceus (informal Trimeresurus puniceus-complex) are revised on the basis of morphological and molecular analyses. Variation in morphological characters were investigated among 119 specimens from 62 populations of the whole range of the pitvipers currently known as Trimeresurus puniceus (Boie, 1827), Trimeresurus borneensis (Peters, 1872) and Trimeresurus brongersmai Hoge, 1969. Molecular and morphological analyses clearly differentiate two groups of taxa, referrable to the informal Trimeresurus puniceus-group and Trimeresurus borneensis-group, and confirm the distinct specific status of T. puniceus and T. borneensis. Morphological univariate and multivariate analyses differentiate six clusters of populations that are morphologically diagnosable, of which five are here considered to represent independent lineages and one is placed incertae sedis pending the availability of further specimens. These clusters are considered to be distinct species following the Biological Species Concept and the Phylogenetic Species Concept. One of them is described as a new species, Trimeresurus andalasensis spec. nov. (T. borneensis-group), which includes populations from northern Sumatra. Trimeresurus wiroti Trutnau, 1981 is revalidated to accommodate populations from Thailand and West Malaysia. Trimeresurus borneensis is here considered endemic to Borneo. Trimeresurus puniceus is known from Java and from South Sumatra, but the taxonomy of this species in Sumatra is left unresolved. Also left unresolved is the taxonomic position of specimens from western Sumatra and the Mentawai Archipelago, and from the Natuna Islands and Anamba Islands. Although belonging to the T. puniceus-group, they show some differences to other specimens of the group. They are not referred to any taxon pending the collection of additional specimens. Lastly, Trimeresurus brongersmai is confirmed as a valid species from the Mentawai Archipelago. A key to these taxa is provided.

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Redescriptions of Eunice filamentosa and E. denticulata and description of E. tovarae n. sp. (Polychaeta: Eunicidae), highlighted with morphological and molecular data

Zootaxa ◽

10.11646/zootaxa.2880.1.5 ◽

2011 ◽

Vol 2880 (1) ◽

pp. 51 ◽

Cited By ~ 4

Author(s):

LUIS F. CARRERA-PARRA ◽

SERGIO I. SALAZAR-VALLEJO

Keyword(s):

Genetic Divergence ◽

Sequence Variation ◽

Molecular Data ◽

Morphological Data ◽

Valid Species ◽

Caribbean Region ◽

Tropical Eastern Pacific ◽

Mexican Pacific ◽

Nucleotide Sequence Variation ◽

Morphological And Molecular Data

In a series of studies on eunicids, three Grand Caribbean species Eunice filamentosa, E. denticulata, and E. conglomerans were regarded as synonyms, or part of a species complex with an amphiamerican distribution. The revision of type and additional materials collected in the Grand Caribbean Region (GCR) and along the Mexican Pacific coasts, allowed us to clarify that E. conglomerans is a junior synonym of E. denticulata. Thus, E. filamentosa and E. denticulata are valid species in the GCR; while the specimens from the Tropical Eastern Pacific belong to a newly described species, E. tovarae n.sp. Herein we describe these three species, and some morphological features described in previous studies are reevaluated. Additionally, we found an important genetic divergence in nucleotide sequence variation of COI, which supported the morphological data. E. filamentosa and E. denticulata have a genetic divergence of 19.6%; whereas E. tovarae n. sp. has a genetic divergence of 20.7% from E. denticulata, and a 12.9% divergence from E. filamentosa.

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A new species of Stenchaetothrips (Thysanoptera, Thripidae) from Bamboo, based on morphological and molecular data

Zootaxa ◽

10.11646/zootaxa.4323.2.13 ◽

2017 ◽

Vol 4323 (2) ◽

pp. 295

Author(s):

LAURENCE A. MOUND ◽

DISNA N. GUNAWARDANA ◽

DONGMEI LI

Keyword(s):

New Species ◽

New Zealand ◽

Southeast Asia ◽

Host Plant ◽

Related Species ◽

Molecular Data ◽

Morphological And Molecular Data ◽

A New Species

Stenchaetothrips martini sp.n. is distinguished from related species on morphological and molecular character states. It is the eleventh species in this genus to be recorded from a species of bamboo, but only the fourth of these 11 species to have a prominent spinula on the mesothoracic furca. Described here from New Zealand, this thrips is presumably introduced from Southeast Asia, together with its host plant, Phyllostachys aurea.

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Morphological and molecular systematic review of Marphysa Quatrefages, 1865 (Annelida: Eunicidae) species from South Africa

PeerJ ◽

10.7717/peerj.10076 ◽

2020 ◽

Vol 8 ◽

pp. e10076

Author(s):

Jyothi Kara ◽

Isabel C. Molina-Acevedo ◽

Joana Zanol ◽

Carol Simon ◽

Izwandy Idris

Keyword(s):

South Africa ◽

Systematic Review ◽

Taxonomic Status ◽

Molecular Data ◽

Valid Species ◽

Molecular Systematic ◽

Widespread Species ◽

Morphological And Molecular Data ◽

Taxonomic Framework ◽

Marphysa Sanguinea

A vast polychaete fauna is hidden behind complexes of cryptic and pseudo-cryptic species, which has greatly hindered our understanding of species diversity in several regions worldwide. Among the eunicids, Marphysa sanguinea Montagu, 1813 is a typical example, recorded in three oceans and with various species considered its junior synonyms. In South Africa, specimens previously misidentified as M. sanguinea are now known as Marphysa elityeni Lewis & Karageorgopoulos, 2008. Of the six Marphysa Quatrefages, 1865a species recorded from the same region, three have their distributions restricted to South Africa while the others are considered to have worldwide distributions. Here, we evaluated the taxonomic status of the indigenous M. elityeni and investigated the presence of the widespread species Marphysa macintoshi Crossland, 1903 and Marphysa depressa Schmarda, 1861 in South Africa using morphological and molecular data. Our results reveal that M. elityeni is a junior synonym of Marphysa haemasoma, a species previously described from South Africa which is herein reinstated as a valid species. Both M. macintoshi and M. depressa are not present in South Africa and their status as being distributed worldwide deserves further investigation. Marphysa durbanensis Day, 1934 and the new species described here, M. sherlockae n. sp., had been misidentified as M. macintoshi and M. depressa respectively. Thus, the number of Marphysa species with distributions restricted to South Africa increased from three to five. This study reiterates the importance of implementing an integrated taxonomic framework to unravel local biodiversity.

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Branching patterns of the afferent branchial arteries and their phylogenetic significance in rays (Batoidea)

Scientific Reports ◽

10.1038/s41598-021-02145-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Karla D. A. Soares ◽

Mônica Toledo-Piza

Keyword(s):

Spatial Relationship ◽

Molecular Data ◽

Data Matrix ◽

Morphological Data ◽

Valid Species ◽

Recent Analysis ◽

Common Trunk ◽

Close Spatial Relationship ◽

Morphological And Molecular Data ◽

Branchial Arteries

AbstractRays of the superorder Batoidea comprise the most diverse group of chondrichthyans in terms of valid species and morphological disparity. Up to the present little agreement is observed in studies based on morphological and molecular data focused on uncovering the interrelationships within Batoidea. Morphology-based phylogenies of batoids have not included characters related to the afferent branchial arteries, and little is known about the variation in this anatomical complex in rays. Herein, representatives of 32 genera from 19 families currently recognized of rays were examined as well as some shark taxa. Seven new characters are proposed and tested in two different analyses, one on their own and in the other they were added to the morphological data matrix of the most recent analysis of interrelationships within Batoidea. The arrangement of afferent branchial arteries differs mainly among orders and families of batoids. The absence of a common trunk from which the three posteriormost afferent arteries branch is interpreted as a synapomorphy for Myliobatiformes and the presence of a coronary cranial artery as an autapomorphy for Mobula hypostoma. A close spatial relationship between the second and third afferent arteries within the common branch from the ventral aorta is proposed as a synapomorphy for Rajiformes with a secondary modification in Sympterygia. Data about patterns in afferent branchial arteries in additional taxa such as Squaliformes and Chimaeriformes are needed to better understand the evolution of this character complex among chondrichthyans.

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Morphological ontogeny and molecular analyses of geographic strains of two closely related Neoseiulus species (Acari: Phytoseiidae)

Acarologia ◽

10.24349/acarologia/20214440 ◽

2021 ◽

Vol 61 (2) ◽

pp. 432-452

Author(s):

Mohamed W. Negm ◽

Tomoko Matsuda ◽

Takumi Kayukawa ◽

Chyi-Chen Ho ◽

Yu-Tzu Hsu ◽

...

Keyword(s):

Ribosomal Dna ◽

Related Species ◽

Molecular Data ◽

Predatory Mites ◽

Nuclear Ribosomal Dna ◽

Immature Stages ◽

Closely Related Species ◽

Molecular Analyses ◽

Neoseiulus Longispinosus ◽

Morphological And Molecular Data

The predatory mites, Neoseiulus longispinosus (Evans) and N. womersleyi (Schicha) (Phytoseiidae) are two morphologically close species. Although they can be mainly separated by the length of the dorsal seta S5, misidentifications may occur. The present study investigated various strains of the two closely related species collected from Japan, Taiwan and/or Thailand using a combination of morphological and molecular data. We described and illustrated the adults and immature stages. We also sequenced the 28S region of nuclear ribosomal DNA to determine whether these strains represent different species. The two species could be clearly separated based on the length of seta S5 and the ratio of setae Z5/S5. In a 28S tree, the examined strains also formed separate clades with 100% bootstrap values. We conclude that a combination of different methods is often necessary to precisely distinguish these closely related taxa.

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