Species Delimitation In Rhabdosciadium (Apiaceae): Morphological and Molecular

Phytotaxa ◽  
2020 ◽  
Vol 437 (1) ◽  
pp. 1-13
Author(s):  
SHAHRAM MEHRI ◽  
IMAN KOLBADI ◽  
HASSAN SHIRAFKAN-AJIRLOU
Keyword(s):  
Species Delimitation ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Mantel Test ◽  
Species Structure ◽  
Genetic Affinity ◽  
Data Set ◽  
Geographical Populations ◽  
Morphological And Molecular Data ◽  
Issr Molecular Markers

Rhabdosciadium is a distinct and small Apiaceae genus represented by only 8 species worldwide, 5 of which are distributed in Turkey and 3 occur in Iran. They all are narrow endemic, rare mountain species. The genus is characterized by single-fruited umbellules, linear-cylindrical fruits, parennial, glabrous, and junciform habit, reduced cauline leaves, and fibrous collar at the base of the stem. Till present time, there has been no detailed information available on molecular phylogeny and genetic structure of these species in the country. Therefore, the present study was conducted with the aim to investigate species delimitation by both morphological and molecular data and to reveal genetic diversity and population structure in these three Rhabdosciadium species. For this study, 98 randomly collected plants from 15 geographical populations in Rhabdosciadium species were used. We encountered extensive within species genetic and morphological diversity. ISSR molecular markers could delimit the studied species. STRUCTURE analysis revealed the occurrence gene flow between these species. The Mantel test showed correlation between genetic distance and geographical distance of the populations studied. Phylogenetic tree was constructed based on ITS data set which separated out-groups from the studied species. Genetic affinity of the studied species have been discussed.

scholarly journals Species delimitation in Capparis (Capparaceae): Morphological and molecular

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 609-627
Author(s):  
Sakine Najafian ◽  
Iraj Mehregan ◽  
Alireza Iranbakhsh ◽  
Mostafa Assadi ◽  
Silvio Fici
Keyword(s):  
Species Delimitation ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Mantel Test ◽  
Joint Disease ◽  
Species Structure ◽  
Data Set ◽  
Geographical Populations ◽  
Capparis Spinosa ◽  
Morphological And Molecular Data

Capparis spinosa L. (Capparidaceae) is the largest genus of the family Capparaceae, distributed in pantropical region. C. spinosa is known as a medicinal plant species. In Iran, different parts of caper bush plants are used as diuretics, tonics and in treatment of malaria and joint disease. Till present time, there has been no detailed information available on molecular phylogeny and genetic structure of these species in the country. Therefore, the present study was conducted with the aim to investigate species delimitation by both morphological and molecular data and to reveal genetic diversity and population structure in these five of Capparis species. For this study, 108 randomly collected plants from 20 geographical populations in of Capparis species were used. We encountered extensive within species genetic and morphological diversity. ISSR molecular markers could delimit the studied species. STRUCTURE analysis revealed the occurrence gene flow between these species. The Mantel test showed correlation between genetic distance and geographical distance of the populations studied. Phylogenetic tree was constructed based on ITS data set which separated out-groups from the studied species. Genetic affinity of the studied species have been discussed.

scholarly journals Gene flow and population structure in Allochrusa (caryophylloideae, caryophyllaceae) with the use of molecular markers

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 799-812
Author(s):  
Weimiao Chen ◽  
Majid Khayatnezhad ◽  
Nima Sarhadi
Keyword(s):  
Population Structure ◽  
Molecular Markers ◽  
Genetic Structure ◽  
Species Delimitation ◽  
Morphological Diversity ◽  
Diagnostic Value ◽  
Mantel Test ◽  
Geographical Distance ◽  
Genetic Affinity ◽  
Geographical Populations

Allochrusa Bunge is a genus of subfamily Caryophylloideae Rabeler & Bittrich contains about 8 species distributed in Turkey, Central Asia, Afghanistan, Caucasus, Transcaucasus and Iran. Three species of Allochrusa versicolor, A. bungei and A. persica occur in Iran show some degree of morphological overlaps that make the species delimitation difficult. Till present time, there has been no detailed information available on morphological and genetic structure of these species in the country. The aims of the present study are: 1) to find the diagnostic value of SCoT markers in delimitation of Allochrusa species, 2) to find the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship. For this study, 97 randomly collected plants from 7 geographical populations in three Allochrusa species were used. We encountered extensive within species genetic and morphological diversity. SCoT molecular markers could delimit the studied species. AMOVA and STRUCTURE analysis revealed that the species of Allochrusa are genetically differentiated. The Mantel test showed correlation between genetic distance and geographical distance of the populations studied. Genetic affinity of the studied species has been discussed.

Species delimitation and population structure in three Onosma (Boraginaceae) species

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Masoud Sheidai ◽  
Samira Sadeghi ◽  
Mahnaz-Arab Ameri ◽  
Ahmad-Reza Mehrabeian
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Species Delimitation ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Morphological Diversity ◽  
Mantel Test ◽  
Geographical Distance ◽  
Nj Tree ◽  
Issr Molecular Markers

AbstractThe present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.

Taxonomic reassessment of the freshwater mussel genus Unio (Bivalvia: Unionidae) in Russia and Ukraine based on morphological and molecular data

Zootaxa ◽  
2017 ◽  
Vol 4286 (1) ◽  
pp. 93 ◽  
Author(s):  
OLGA KLISHKO ◽  
MANUEL LOPES-LIMA ◽  
ELSA FROUFE ◽  
ARTHUR BOGAN ◽  
LYUDMILA VASILIEV ◽  
...  
Keyword(s):  
Freshwater Mussel ◽  
Integrated Approach ◽  
Molecular Data ◽  
Coi Gene ◽  
Data Set ◽  
Widespread Species ◽  
Molecular Analyses ◽  
Morphological Variations ◽  
Mtdna Coi ◽  
Morphological And Molecular Data

The taxonomy of species within the genus Unio (Bivalvia: Unionidae: Unioninae) in Russia and Ukraine has been contentious due to the lack of correspondence between three concurrent yet divergent classifications. In order to clarify which classification system best reflects the evolutionary relationships among these taxa, we performed detailed morphological analyses on 720 Ukrainian and Russian specimens, complemented with molecular data (COI) from a selected number of specimens. The morphological character data set shows the existence of only three widespread species with slight eco-morphological variations. Statistical analyses of shell morphometric parameters and molecular analyses based on mtDNA COI gene fragment sequences confirm the existence of the same three species within a single genus, Unio, in Russia and Ukraine, that is U. pictorum, U. tumidus and U. crassus. Results from molecular analyses suggest the existence of an additional subgroup within the U. crassus lineage, U. crassus cf. courtilieri that deserves further research. The present integrated approach confirms the validity of the classification by Zhadin (1952) and rejects the complex classifications of Starobogatov et al. (2004) and Bogatov & Kijashko (2016). 

Phylogenetic Relationships of Species of Hypselobarbus (Cypriniformes: Cyprinidae): An Enigmatic Clade Endemic to Aquatic Systems of India

Zootaxa ◽  
2012 ◽  
Vol 3499 (1) ◽  
pp. 63
Author(s):  
M. ARUNACHALAM ◽  
M. RAJA ◽  
M. MURALIDHARAN ◽  
RICHARD L. MAYDEN
Keyword(s):  
Genetic Diversity ◽  
Morphological Study ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Mitochondrial Genes ◽  
Morphological Evidence ◽  
Species Relationships ◽  
Peninsular India ◽  
Morphological And Molecular Data ◽  
Eleven Species

Very little is known about the diversity and systematics of the genus cypriniform genus Hypselobarbus. Currently, the genusincludes at least eleven species, all endemic to freshwater systems of Peninsular India. While these species are commonlyknown in India and are frequently used as a food source, little is known about the morphological diversity within and betweenspecies and nothing is known regarding intraspecific genetic diversity or species relationships. Herein, we examine the geneticdiversity in the genus for 11 mitochondrial genes for eleven populations representing nine of the known 11 species.Hypselobarbus is resolved as monophyletic, with the inclusion of P. carnaticus, and species relatioships are very stronglysupported. Because of the unambiguous relationships strongly supported B. carnaticus is allocated to Hypselobarbus. Thisresearch and ongoing morphological and molecular work with the genus supports the existence of additional new species inpeninsular India in need of further molecular and morphological study. Genetic diversity in the genus is high; for the twospecies wherein more than one sample, and the two of each are suspected to represent undescribed taxa, these populationsexhibited greater genetic divergence than that observed between any two of the other currently recognized species,corroborating our hypothesis based on morphological evidence. Clearly the genus warrants more thorough geographicsampling and examination of morphological and molecular data/analyses to reveal the natural lineages existing in this endemic and enigmatic genus.

scholarly journals A revision of Onychelmis Hinton, 1941 (Coleoptera: Elmidae), with description of new species, DNA barcoding and notes on the geography of the genus

2021 ◽  
Vol 739 ◽  
pp. 1-35
Author(s):  
Marek Linský ◽  
Zuzana Čiamporová-Zaťovičová ◽  
Fedor Čiampor Jr
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Species Delimitation ◽  
Molecular Data ◽  
Distribution Map ◽  
Line Drawings ◽  
The Andes ◽  
Long Time ◽  
New Material ◽  
Morphological And Molecular Data

The genus Onychelmis Hinton, 1941 was for a long time regarded as a small taxon with only three known species distributed in the Andes. A study of new material from Ecuador, using morphological and molecular data, has resulted in the discovery of five new species: Onychelmis lenkae sp. nov., O. lobata sp. nov., O. minor sp. nov., O. onorei sp. nov. and O. splendida sp. nov. We also revised the entire genus and redescribed the three known species, O. longicollis (Sharp, 1882), O. leleupi Delève, 1968 and O. whiteheadi Spangler & Santiago, 1991. Habitus photographs of adults are provided, together with line drawings of male and female genitalia, and schematic illustrations of the distribution of femoral tomentum for each species. DNA sequences for barcoding the COI mtDNA fragment were used to support species delimitation and to suggest possible relationships among species. The revision includes a key to adults of all species of Onychelmis and notes on the biogeography of the genus, with an updated distribution map.

Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the “Crangonyx floridanus complex”

Zootaxa ◽  
2019 ◽  
Vol 4691 (4) ◽  
pp. 301-332 ◽  
Author(s):  
ANDREW G. CANNIZZARO ◽  
THOMAS R. SAWICKI
Keyword(s):  
New Species ◽  
Related Species ◽  
Species Delimitation ◽  
Species Complex ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Closely Related Species ◽  
Leon County ◽  
Two New Species ◽  
Morphological And Molecular Data

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses. 

scholarly journals First characterisation of minor and neglected Vitis vinifera L. cultivars from Mount Etna

2018 ◽  
Vol 45 (No. 1) ◽  
pp. 37-46
Author(s):  
Filippo Ferlito ◽  
Elisabetta Nicolosi ◽  
Stefano La Malfa ◽  
Antonio Cicala ◽  
Alessandra Gentile
Keyword(s):  
Vitis Vinifera ◽  
Ssr Markers ◽  
Agronomic Traits ◽  
Germplasm Conservation ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Mount Etna ◽  
Conservation Strategy ◽  
Vitis Vinifera L ◽  
Morphological And Molecular Data

Eight minor and neglected cultivars of Vitis vinifera L. were characterised according to their ampelographic and agronomic traits and discriminated by molecular analysis with SSR markers. These results are the first complete ampelographic description of these minor and neglected cultivars from the Mount Etna region. The results of this study reveal high morphological diversity of ancient grapevines growing in this region. SSR markers enabled us to discriminate the cultivars and revealed the genetic divergence between them and several autochthonous cultivars. Our efforts could contribute to a better knowledge of grape biodiversity based on morphological and molecular data and could be useful for the development of a reliable germplasm conservation strategy. 

Phylogenetic analysis of the druid flies (Diptera: Schizophora: Clusiidae) based on morphological and molecular data

2010 ◽  
Vol 41 (3) ◽  
pp. 231-274 ◽  
Author(s):  
Jinzhong Fu ◽  
Owen Lonsdale ◽  
Brian Wiegmann ◽  
Stephen Marshall
Keyword(s):  
Cytochrome Oxidase ◽  
Dna Sequences ◽  
Phylogenetic Reconstruction ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Cytochrome Oxidase Subunit ◽  
Data Set ◽  
Oxidase Subunit ◽  
Morphological And Molecular Data

AbstractIn this paper, the Clusiidae (Diptera: Schizophora) is analyzed phylogenetically using morphological and molecular data sets, and then redefined on the basis of derived morphological characters. The biology and distribution of the Clusiidae are also reviewed, a key is provided to the World genera, the status of the genus Craspedochaeta Czerny is reevaluated and the type of Heterochroa pictipennis Wulp is discussed. Molecular data sets include genomic DNA sequences from the mitochondrial genes COI (cytochrome oxidase subunit I) and COII (cytochrome oxidase subunit II), the large ribosomal nuclear subunit 28S, and the nuclear protein-coding carbomoylphosphate synthase (CPS) domain of CAD (or “rudimentary”). Genes were analyzed separately, in combination with each other, and in combination with a morphological data set. Although individual molecular data sets often provided conflicting phylogenetic signals, the topologies of the cladograms produced from each data set alone or in combination were largely similar. Most genus-level relationships and several basal divergences were unresolved, but Apiochaeta was very strongly and consistently supported as Sobarocephalinae, not Clusiinae. The Clusiinae and Sobarocephalinae are subsequently redefined using an adjusted morphological tree — retaining Apiochaeta in the Sobarocephalinae — that is only slightly longer (8.4%, or seven steps) than the most parsimonious tree. Our results illustrate the benefits of multiple independent data sets for phylogenetic reconstruction in order to verify and refine existing classifications.

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