Hepatozoon langii n. sp. and Hepatozoon vacuolatus n. sp. (Apicomplexa: Adele­orina: Hepatozoidae) from the crag lizard (Sauria: Cordylidae) Pseudocordylus langi from the North Eastern Drakensberg escarpment, Eastern Free State, South Africa

Zootaxa ◽  
2013 ◽  
Vol 3608 (5) ◽  
pp. 345-356 ◽  
Author(s):  
JOHANN VAN AS ◽  
ANGELA J. DAVIES ◽  
NICO J. SMIT
Keyword(s):  
South Africa ◽  
High Altitude ◽  
The Other ◽  
Host Cells ◽  
Giemsa Staining ◽  
Free State ◽  
Mixed Infections ◽  
The North ◽  
North Eastern ◽  
Eastern Free State

Two new haemogregarine species, Hepatozoon langii n. sp. and Hepatozoon vacuolatus n. sp., are described from the pe-ripheral blood of the high altitude crag lizard, Pseudocordylus langi, collected between October 2006 and April 2009 from the North Eastern Drakensberg, Eastern Free State. Hepatozoon langii n. sp. has maturing and mature gamonts that appear encapsulated and have narrow, curved tails. Their cytoplasm stains pinkish-purple with Giemsa, while their nuclei are pur-ple stained with stranded chromatin. Mature gamonts measure 19.1 ± 1.0 (15.4–28.1) μm long by 6.2 ± 1.1 (3.5–7.9) μm wide. Hepatozoon vacuolatus n. sp. gamonts are mostly broader at one pole than the other, have bluish-pink cytoplasm characterised by distinctive rounded and oval vacuoles, and demonstrate pink granules with Giemsa staining. Nuclei stain purple and are mainly coarsely granular. Mature gamonts measure 16.5 ± 1.0 (14.7 - 17.6) μm long by 5.9 ± 1.2 (4.0 - 7.7) μm wide. Both species parasitize erythroblasts, as well as erythrocytes and can dehaemoglobinize the cytoplasm of their host cells. Hepatozoon langii n. sp occurred in the absence of H. vacuolatus n. sp., but the latter haemogregarine always formed mixed infections with the former; no stages intermediate between the two haemogregarine types were observed.

scholarly journals Prevalence of Theileria equi and Babesia caballi infections in horses belonging to resource-poor farmers in the north-eastern Free State Province, South Africa

2008 ◽  
Vol 75 (2) ◽  
Author(s):  
M. Y. Motloang ◽  
O. M.M. Thekisoe ◽  
A. Alhassan ◽  
M. Bakheit ◽  
M. P. Motheo ◽  
...  
Keyword(s):  
South Africa ◽  
Free State ◽  
Serum Samples ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Free State Province ◽  
The North ◽  
North Eastern ◽  
Study Sites ◽  
Eastern Free State

The prevalence of Theileria equi and Babesia caballi infections in the north-eastern Free State Province of South Africa was determined by examination of thin and thick Giemsa-stained blood smears, IFAT and PCR. No parasites were detected by microscopy from any blood samples collected at five study sites, Qwaqwa, Kestell, Harrismith, Vrede and Warden. Of the tested serum samples, 28/29 (96.5%), 20/21 (95.2%) nd 42/42( 100%) were positive by IFAT for T. equi infections in Harrismith, Kestell and Qwaqwa, respectively, and 5/29 (17.2%), 13/21 (61.9%) and 30/42 (71.4%) were sero-positive for B. caballi infections in Harrismith, Kestell and Qwaqwa, respectively A. ll DNA samples from the study sites were negative for B. caballi infections by PCR, but five samples, two from each of Kestell and Warden and one from Vrede, were PCR positive for T. equi infections. The high prevalence of antibodies against T. equi and B. caballiin the sampled horses indicates that the animals had been exposed to T. equi and B. caballi infections but the absence of parasitaemia and very low number of positive PCR samples, however, imply that T. equi and B. caballie are endemically stable in the north-eastern Free State Province.

scholarly journals A sero-epidemiological survey of blood parasites in cattle in the north-eastern Free State, South Africa

2004 ◽  
Vol 71 (1) ◽  
Author(s):  
M.S. Mtshali ◽  
D.T. De Waal ◽  
P.A. Mbati
Keyword(s):  
Fluorescent Antibody ◽  
Boophilus Microplus ◽  
Free State ◽  
Blood Parasites ◽  
Enzyme Linked Immunosorbent Assay ◽  
Babesia Bigemina ◽  
The North ◽  
Blood Smears ◽  
North Eastern ◽  
Eastern Free State

A survey to determine the incidence of parasites in cattle (n = 386) was conducted in the north eastern Free State between August 1999 and July 2000. Giemsa-stained blood smears were negative for blood parasites. A total of 94 % of the cattle were sero-positive for Babesia bigemina by indirect fluorescent antibody test while 87 % were sero-positive for Anaplasma by enzyme-linked immunosorbent assay. The observation of negative blood smears but high incidence of positive serological results for Anaplasma and Babesia for the same group of cattle indicates that this area is endemic for these diseases but with a stable disease situation. All the animals were sero-negative for B. bovis and this is probably because the tick vector (Boophilus microplus) which transmits the disease is not present in the Free State Province. Two tick species belonging to the family Ixodidae were found on cattle, namely Boophilus decoloratus and Rhipicephalus evertsi evertsi. In the present study significant differences in seasonal burdens of B. decoloratus occurred, with the highest infestations recorded from February to June. The presence of R. evertsi evertsi throughout the year without any or with small fluctuations in winter months was observed, with a peak from February to May

On Probstmayria reptiliae n. sp., from Homopus femoralis and Some Notes on the Genus Probstmayria

1958 ◽  
Vol 32 (4) ◽  
pp. 211-218 ◽  
Author(s):  
W. M. Fitzsimmons
Keyword(s):  
South Africa ◽  
Alimentary Canal ◽  
The Other ◽  
Free State ◽  
The Subject

This worm was very abundant in the rectum of a tortoise, Homopus femoralis, from the Bloemfontein area, Orange Free State, South Africa. It was found together with several other oxyurids the description of which will form the subject of a later paper. Although the Probstmayria far exceeded the other oxyurids in number, male specimens of this genus were extremely rare for only one male was found after a prolonged search during the course of which many hundreds of females were encountered. In order that no specimens should be lost the entire alimentary canal together with its contents was submerged in hot 70% alcohol after scraping the mucosa.

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