scholarly journals Fate and Effects of Adult Bone Marrow Cells in Lungs of Normoxic and Hyperoxic Newborn Mice

2009 ◽  
Vol 40 (5) ◽  
pp. 575-587 ◽  
Author(s):  
James A. Fritzell ◽  
Quanfu Mao ◽  
Sravanthi Gundavarapu ◽  
Terry Pasquariello ◽  
Jason M. Aliotta ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Newborn Mice ◽  
Adult Bone Marrow ◽  
Adult Bone ◽  
Marrow Cells

scholarly journals The Characterization, Molecular Cloning, and Expression of a Novel Hematopoietic Cell Antigen From CD34+ Human Bone Marrow Cells

Blood ◽  
1997 ◽  
Vol 89 (8) ◽  
pp. 2706-2716 ◽  
Author(s):  
Nobuko Uchida ◽  
Zhi Yang ◽  
Jesse Combs ◽  
Olivier Pourquié ◽  
Megan Nguyen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Molecular Cloning ◽  
Bone Marrow Cells ◽  
Hematopoietic Cell ◽  
Cell Antigen ◽  
Adult Bone Marrow ◽  
Cd34 Cells ◽  
Adult Bone ◽  
Marrow Cells ◽  
Myeloid Progenitors

Abstract The adhesion molecule BEN/SC1/DM-GRASP (BEN) is a marker in the developing chicken nervous system that is also expressed on the surface of embryonic and adult hematopoietic cells such as immature thymocytes, myeloid progenitors, and erythroid progenitors. F84.1 and KG-CAM, two monoclonal antibodies to rat neuronal glycoproteins with similarity to BEN, cross-react with an antigen on rat hematopoietic progenitors, but F84.1 only also recognizes human blood cell progenitors. We have defined the antigen recognized by F84.1 as the hematopoietic cell antigen (HCA). HCA expression was detected on 40% to 70% of CD34+ fetal and adult bone marrow cells and mobilized peripheral blood cells. Precursor cell activity for long-term in vitro bone marrow cell culture was confined to the subset of CD34+ cells that coexpress HCA. HCA is expressed by the most primitive subsets of CD34+ cells, including all rhodamine 123lo, Thy-1+, and CD38−/lo CD34+ adult bone marrow cells. HCA was also detected on myeloid progenitors but not on early B-cell progenitors. We also describe here the cloning and characterization of cDNAs encoding two variants of the human HCA antigen (huHCA-1 and huHCA-2) and of a cDNA clone encoding rat HCA (raHCA). The deduced amino acid sequences of huHCA and raHCA are homologous to that of chicken BEN. Recombinant proteins produced from either human or rat HCA cDNAs were recognized by F84.1, whereas rat HCA but not human HCA was recognized by antirat KG-CAM. Expression of either form of huHCA in CHO cells conferred homophilic adhesion that could be competed with soluble recombinant huHCA-Fc. The molecular cloning of HCA and the availability of recombinant HCA should permit further evaluation of its role in human and rodent hematopoiesis.

scholarly journals Thrombopoietin Is Synthesized by Bone Marrow Stromal Cells

Blood ◽  
1997 ◽  
Vol 90 (9) ◽  
pp. 3444-3455 ◽  
Author(s):  
Anastasia Guerriero ◽  
Lydia Worford ◽  
H. Kent Holland ◽  
Gui-Rong Guo ◽  
Kevin Sheehan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Cell Sorting ◽  
Bone Marrow Cells ◽  
Adult Bone Marrow ◽  
Hla Dr ◽  
Adult Bone ◽  
Marrow Cells

Abstract We have previously characterized stromal progenitor cells contained in fetal bone marrow by fluorescence-activated cell sorting (FACS) using the differential expression of CD34, CD38, and HLA-DR, and found that a small number were contained within the CD34+ cell fraction. In the present study, the frequency of stromal progenitors in both the CD34+ and CD34− subpopulations from samples of fetal and adult bone marrow was approximately one in 5,000 of the mononuclear cell fraction. Using multiparameter single-cell sorting, one in 20 fetal bone marrow cells with the CD34+, CD38−, HLA-DR−, CDw90+ phenotype were clonogenic stromal progenitors, whereas greater than one in five single cells with the CD34−, CD38−, HLA-DR−, CDw90+ phenotype formed stromal cultures. We found that cultures initiated by hematopoietic and stromal progenitors contained within the CD34+ fraction of bone marrow cells formed mixed hematopoietic/stromal cell cultures that maintained the viability of the hematopoietic progenitor cells for 3 weeks in the absence of added hematopoietic cytokines. We characterized some of the hematopoietic cytokines synthesized by stromal cultures derived from either CD34+ or CD34− bone marrow cells using reverse transcriptase–polymerase chain reaction (RT-PCR) amplification of interleukin-3 (IL-3), stem cell factor (SCF), CD34, Flt3/Flk2 ligand (FL), and thrombopoietin (TPO) mRNA sequences. We found ubiquitous expression of TPO mRNA in greater than 90% of stromal cultures initiated by either CD34+ or CD34− cells, and variable expression of SCF, FL, and CD34 mRNA. In particular, SCF and CD34 mRNA were detected only in stromal cultures initiated by CD34+ bone marrow cells, although the differences between CD34+ and CD34− stromal cells were not statistically significant. IL-3 mRNA was not found in any stromal cultures. An enzyme-linked immunosorbent assay (ELISA) of soluble SCF and TPO present in culture supernatants demonstrated that biologically significant amounts of protein were secreted by some cultured stromal cells: eight of 16 samples of conditioned media from stromal cultures initiated by fetal and adult bone marrow contained more than 32 pg/mL SCF (in the linear range of the ELISA), with a median value of 32 pg/mL (range, 9 to 230), while 13 of 24 samples of conditioned media had more than 16 pg/mL TPO (in the linear range of the ELISA), with a median of 37 pg/mL (range, 16 to 106). Our data indicate that stromal cultures initiated by single bone marrow cells can make FL, SCF, and TPO. Local production of early-acting cytokines and TPO by stromal cells may be relevant to the regulation of hematopoietic stem cell self-renewal and megakaryocytopoiesis in the bone marrow microenvironment.

Intra-Arterial Transplantation of Adult Bone Marrow Cells Restores Blood Flow and Regenerates Skeletal Muscle in Ischemic Limbs

2009 ◽  
Vol 43 (5) ◽  
pp. 433-443 ◽  
Author(s):  
Qi Liu ◽  
Zhiqiang Chen ◽  
Toya Terry ◽  
Janice M. McNatt ◽  
James T. Willerson ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Bone Marrow ◽  
Blood Flow ◽  
Bone Marrow Cells ◽  
Adult Bone Marrow ◽  
Adult Bone ◽  
Marrow Cells

scholarly journals Monoclonal origin of B lymphocyte colony-forming cells in spleen colonies formed by multipotential hemopoietic stem cells

1978 ◽  
Vol 148 (6) ◽  
pp. 1468-1477 ◽  
Author(s):  
PK Lala ◽  
GR Johnson
Keyword(s):  
Bone Marrow ◽  
B Lymphocyte ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Absolute Number ◽  
Hemopoietic Stem Cell ◽  
Adult Bone Marrow ◽  
Clonal Origin ◽  
Adult Bone ◽  
Marrow Cells

Spleen colonies produced by transplanting lethally irradiated mice with either 12 day fetal liver or adult bone marrow cells were found to contain B- lymphocyte colony-forming cells (BL-CFC) . The proportion of BL-CFC positive spleen colonies did not increase substantially between 8 and 14 days after transplantation, the range being 18-45 percent. However, the absolute number of BL-CFC per spleen colony varied considerably (between 1 and 10,318), although the majority of colonies contained less than 200 BL-CFC. Irrespective of the time after transplantation, smaller spleen colonies were found to have a higher frequency of BL-CFC than larger spleen colonies. To determine the possible clonal origin of BL-CFC from spleen colony- forming unit (CFU-S), CBA mice were injected with equal numbers of CBA and CBA T(6)/T(6) fetal liver or adult bone marrow cells. Analysis of 7-15-day spleen colonies demonstrated that 90 percent were either exclusively T(6) positive or T(6) negative and approximately equal numbers ofboth colony types were observed. B-lymphocyte colonies were grown and successfully karyotyped from 19 spleen colonies. When compared with the original spleen colony karyotype the B-lymphocyte colony cells karyotype was identical in all 19 cases. In 3 of the 19 colonies analyzed a mixture of T(6) positive and T(6) negative karyotypes was present and identical proportions of the karyotypes were present in the pooled B-lymphocyte colony cells and spleen colony cells. The data indicate that the B-lymphocyte colony-forming cells detected in spleen colonies are genuine members of the hemopoietic clone derived from the initiating hemopoietic stem cell (CFU-S).

Generation of Induced Pluripotent Stem Cells by Efficient Reprogramming of Adult Bone Marrow Cells

2010 ◽  
Vol 19 (2) ◽  
pp. 229-238 ◽  
Author(s):  
Atsushi Kunisato ◽  
Mariko Wakatsuki ◽  
Yuuki Kodama ◽  
Haruna Shinba ◽  
Isao Ishida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Marrow Cells ◽  
Adult Bone Marrow ◽  
Induced Pluripotent ◽  
Adult Bone ◽  
Marrow Cells

scholarly journals In vitro tolerance induction of neonatal murine B cells as a probe for the study of B-cell diversification.

1977 ◽  
Vol 145 (5) ◽  
pp. 1382-1386 ◽  
Author(s):  
E S Metcalf ◽  
N H Sigal ◽  
N R Klinman
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
B Cell ◽  
Bone Marrow Cells ◽  
Tolerance Induction ◽  
Adult Bone Marrow ◽  
Late Expression ◽  
Adult Bone ◽  
Marrow Cells

The susceptibility to in vitro tolerance induction has been implicated as a characteristic of B cells early in their development, since DNP-reactive B cells are tolerizable only during the first days after birth, and 25% of adult bone marrow cells are tolerizable. In the present study, a modification of the in vitro splenic focus technique was utilized to determine if PC-specific B cells, by virtue of their late expression (approximately 1 wk post-parturition), also display susceptibility to tolerance induction. The results demonstrate that at 7-10 days after birth, when over 90% of the DNP-specific splenic B cells are resistant to tolerance induction, the majority of PC-specific B cells are tolerizable. These results re-emphasize tolerance susceptibility as a characteristic of developing clones, confirm the late acquisition of PC-specific B cells, and support the contention that the acquisition of the specificity repertoire is a highly ordered, specifically predetermined process which is independent of antigen-driven events.

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