Rhamnetin Attenuates Oxidative Stress and Matrix Metalloproteinase in Animal Model of Ischemia/Reperfusion: A Possible Antioxidant Therapy in Stroke

2013 ◽  
Vol 5 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Anand Kumar Pandey ◽  
Pallab Bhattacharya ◽  
Sudip Paul ◽  
Ranjana Patnaik
Keyword(s):  
Oxidative Stress ◽  
Animal Model ◽  
Matrix Metalloproteinase ◽  
Ischemia Reperfusion ◽  
Antioxidant Therapy

scholarly journals A Randomized clinical trial of antioxidant therapy in patients with septic shock. Reference study to propose adjuvant therapy in patients with critical organic damage by COVID-19

2020 ◽  
Author(s):  
Alfredo Aisa-Alvarez ◽  
Israel Perez-Torres ◽  
Gilberto Camarena-Alejo ◽  
Juvenal Franco-Granillo ◽  
Enrique Martínez-Rodríguez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Clinical Trial ◽  
Septic Shock ◽  
Organ Failure ◽  
Randomized Clinical Trial ◽  
Ischemia Reperfusion ◽  
Research Question ◽  
Antioxidant Therapy ◽  
Random Allocation ◽  
Vit C

Abstract Background: Oxidative stress (OS) participates in the pathophysiology of patients with septic shock having multiple organ failure (MOF), ischemia-reperfusion injury and acute respiratory failure syndrome (ARDS). Antioxidants have been proposed in their therapy.Objective: To evaluate the effect of antioxidant treatment in patients with septic shock with MOF and levels OS after treatment.Research question: Will the administration of specific antioxidant therapy decrease deregulatory factors of oxidative stress and organ failure in patients with septic shock?Study design and Methods: Double-blind, placebo-controlled randomized clinical trial run in 2 ICU in Mexico City between May 2018 and January 2020. The random allocation sequence was generated using computer methods. Patients older than 18 years of either sex, with septic shock were included, were excluded when informed consent could not be obtained, they received chronic or recent use of steroids, statins, or antioxidants or if they had contraindications to the use of antioxidants. All antioxidants were administered by mouth or nasogastric tube during 5 days and were added to standard.Results: 97 patients were included with median age of 66 years. 20 were treated with MT and 18 with Vit C and they showed post-treatment decreased SOFA scores [p=0.007 and p<0.001 respectively]. Also, total antioxidant capacity (TAC) was improved by NAC. All patients had decreased basal levels of Vit C and patients that received Vit C had decreased levels of the NO3−/NO2− (p=0.02) and RCP levels [p=0.045]. Procalcitonin (PCT) levels were reduced by Vit E, [p=0.047], NAC; [p=0.001] and MT [p=0.045]. LPO was reduced in patients that received MT p=0.042 Conclusion: In septic shock, antioxidant therapy associated with standard intensive care unit therapy reduces MOF, the oxidative and inflammatory state. These results could be a reference to use adjuvant antioxidant therapy in patients with septic shock in COVID19.Trial Registration: ClinicalTrials.gov Identifier: NCT 03557229

scholarly journals Effect of protocatechuic acid against renal ischemia reperfusion damage on extracellular matrix integrity and related signal pathways

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Fatma Yıldız ◽  
Hakan Şentürk ◽  
Mustafa Uyanoğlu
Keyword(s):  
Oxidative Stress ◽  
Matrix Metalloproteinase ◽  
Protein Expression ◽  
Protocatechuic Acid ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Matrix Metalloproteinase 2 ◽  
Signal Pathways ◽  
Expression Levels ◽  
Group I

AbstractObjectiveIn this study, possible protective effects of protocatechuic acid (PCA) against experimentally-induced acute renal ischemia/reperfusion (I/R) damage in rats, on matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and the associated signal transduction pathways were investigated.MethodsA total of 3–4 month-old, 200–250 g Sprague Dawley rats were divided into groups of five (n=7). A right kidney nephrectomy surgery was conducted to all groups under anesthesia. Rats were administered polyethylene glycol 1 h prior to ischemia (Group I, II) and PCA (Group III, IV, V) intraperitoneally. Forty five minutes before the ischemia during 24 h reperfusion on all rats except those in Group I. At the end of the experiment, blood urea nitrogen (BUN), creatinine values and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme levels were investigated in blood serum. MMP-2 and MMP-9 gene expression levels were determined by RT-PCR, and p38 and p-p38 protein expression levels Western blotting method. Renal tissue was examined histologically and immunohistochemically.ResultsIt is assumed that 80 and 120 mg/kg of PCA might have a protective effect against oxidative stress damage caused by renal I/R.ConclusionIn our study, PCA has been shown to modulate the increased expression of MMP-2 and MMP-9 mRNA along with increased oxidative stress during renal I/R, as well as oxidative damage-induced p38 protein expression. It was determined that particularly 120 mg kg−1 PCA reduced the renal I/R injury at a rate of 35–45%.

Effects of Off-Pump Versus On-Pump Coronary Artery Bypass Grafting: Apoptosis, Inflammation, and Oxidative Stress

2014 ◽  
Vol 17 (5) ◽  
pp. 271 ◽  
Author(s):  
Murat Bicer ◽  
Tunay Senturk ◽  
Murat Yanar ◽  
Ahmet Tutuncu ◽  
Arzu Yilmaztepe Oral ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ischemia Reperfusion ◽  
Artery Bypass ◽  
Bypass Grafting ◽  
Artery Bypass Grafting ◽  
Cabg Surgery ◽  
Off Pump ◽  
Pump Cabg ◽  
Hs Crp ◽  
And Oxidative Stress

<strong>Background</strong>: It has been suggested that off-pump coronary<br />artery bypass grafting (CABG) surgery reduces myocardial<br />ischemia-reperfusion injury, postoperative systemic<br />inflammatory response, and oxidative stress. The aim of this<br />study was to measure serum malondialdehyde (MDA), highsensitivity<br />C-reactive protein (hs-CRP), M30, and M65 levels<br />and to investigate the relationship between M30 levels and<br />oxidative stress and inflammation in patients undergoing onand<br />off-pump CABG surgery.<br /><strong>Methods</strong>: Fifty patients were randomly assigned to onpump<br />or off-pump CABG surgery (25 patients off-pump and<br />25 on-pump CABG surgery), and blood samples were collected<br />prior to surgery, and 30 minutes, 60 minutes, 6 hours,<br />and 24 hours after CABG surgery.<br /><strong>Results</strong>: Compared to the on-pump group, serum MDA<br />levels at 30 minutes, 60 minutes, 6 hours, and 24 hours after<br />the CABG surgery were significantly lower in the off-pump<br />group (P = .001, P = .001, P = .001, and P = .001, respectively).<br />Serum M30 levels were found to be elevated in both groups,<br />returning to baseline at 24 hours. When compared to baseline,<br />the hs-CRP level reached its peak at 24 hours at 13.28 ±<br />5.32 mg/dL in the on-pump group, and 15.44 ± 4.02 mg/dL<br />in the off-pump group.<br /><strong>Conclusion</strong>: CABG surgery is associated with an increase<br />in inflammatory markers and serum M30 levels, indicating<br />epithelial/endothelial apoptosis in the early period.

Effects of Genipin at NO synthesis and Ischemia-Reperfusion Induced Oxidative Stress in Old Rat Hearts

2010 ◽  
Vol 1 (4) ◽  
pp. 335-344 ◽  
Author(s):  
Yulia V. Goshovska ◽  
Yulia P. Korkach ◽  
Tatiana V. Shimanskaya ◽  
Anatolii V. Kotsuruba ◽  
Vadym F. Sagach
Keyword(s):  
Oxidative Stress ◽  
Ischemia Reperfusion ◽  
Rat Hearts
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