Direct Electrochemistry of Cytochrome c on Graphene Aerogel/Gold Nanoparticle Hybrid for Hydrogen Peroxide Biosensing

2015 ◽  
Vol 9 (5) ◽  
pp. 330-334 ◽  
Author(s):  
Bojun Wang ◽  
Shancheng Yan ◽  
Zixia Lin ◽  
Yi Shi ◽  
Dong Hu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cytochrome C ◽  
Gold Nanoparticle ◽  
Direct Electrochemistry ◽  
Graphene Aerogel

Direct electrochemistry of cytochrome c immobilized on a graphene oxide–carbon nanotube composite for picomolar detection of hydrogen peroxide

RSC Advances ◽  
2014 ◽  
Vol 4 (54) ◽  
pp. 28229-28237 ◽  
Author(s):  
Bose Dinesh ◽  
Veerappan Mani ◽  
Ramiah Saraswathi ◽  
Shen-Ming Chen
Keyword(s):  
Hydrogen Peroxide ◽  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Cytochrome C ◽  
Direct Electrochemistry ◽  
Multiwalled Carbon Nanotube ◽  
Amperometric Biosensor ◽  
Multiwalled Carbon ◽  
Carbon Nanotube Composite

A new bionanocomposite electrode based on cytochrome c immobilized graphene oxide-multiwalled carbon nanotube is used to fabricate a highly selective and sensitive amperometric biosensor for the picomolar level detection of hydrogen peroxide which may find application in bioimaging and healthcare

scholarly journals Macrophage variants in oxygen metabolism.

1980 ◽  
Vol 152 (4) ◽  
pp. 808-822 ◽  
Author(s):  
G Damiani ◽  
C Kiyotaki ◽  
W Soeller ◽  
M Sasada ◽  
J Peisach ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cytochrome C ◽  
Superoxide Anion ◽  
Oxygen Metabolism ◽  
Hexose Monophosphate ◽  
Phagocytic Cells ◽  
Form Complex ◽  
Hexose Monophosphate Shunt ◽  
Cytochrome C Reduction ◽  
Generate Superoxide Anion

Whereas phagocytic cells from normal individuals have the capacity to kill ingested bacteria and parasites, those from patients with several uncommon genetic deficiency diseases are known to be defective in bactericidal activity. Studies on neutrophils of these patients have revealed fundamental defects in their ability to reduce molecular oxygen and metabolize it to superoxide anion, hydrogen peroxide, and oxygen radicals. In the present experiments, we describe a clone of a continuous murine macrophage-like cell line, J774.16, that, upon appropriate stimulation, activates the hexose monophosphate shunt, and produces superoxide anion and hydrogen peroxide. With nitroblue tetrazolium to select against cells capable of being stimulated by phorbol myristate acetate to reduce the dye to polymer--formazan--which is toxic fot cells, we have selected for variants that are defective in oxygen metabolism. Four of these subclones have been characterized and found to be lacking in the ability (a) to generate superoxide anion, as measured by cytochrome c reduction; (b) to produce hydrogen peroxide, as measured by the ability to form complex I with cytochrome c peroxidase; and (c) to be stimulated to oxidize glucose via the hexose monophosphate shunt. These variants appear to represent a useful model for studying the molecular basis for macrophage cytocidal activity.

Gold nanoparticle-composite nanofibers for enzymatic electrochemical sensing of hydrogen peroxide

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 5025 ◽  
Author(s):  
Anitha Devadoss ◽  
Hyungkyu Han ◽  
Taeseup Song ◽  
Young-Pil Kim ◽  
Ungyu Paik
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticle ◽  
Composite Nanofibers ◽  
Electrochemical Sensing
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