Enhanced Cultivation of Hair Follicle Outer Root Sheath Cells with K + M Culture Medium

Hair follicle stem cells are vital seed cells for hair follicle and tissue engineering skin. However, there is no valid approach to obtain abundant cells within short times in vitro. The purpose of this study was to establish a new medium for culture and expansion of hair follicle outer root sheath (ORS) cells. ORS cells were harvested from human samples and cultured with a medium mixed with keratinocyte serum free medium and mouse embryonic fibroblasts suspension. The proliferation and vitality of ORS cells were evaluated. The cells exhibited typical cobblestone morphology with good adhesion and colony-forming ability. The cells were also characterized through detecting specific markers with immunofluorescent staining and the results showed that partial ORS cells could express hair follicle stem cell markers of CK15, CK19 and FST. At last, ORS cells and fibroblasts were used as seed cells and preliminarily constructed tissue engineering skin successfully. In conclusion, these findings suggested that this new medium could effectively accelerate cell proliferation and maintain the fundamental characteristics of ORS cells.

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Enhanced Proliferation and Functions of In Vitro Expanded Human Hair Follicle Outer Root Sheath Cells by Low Oxygen Tension Culture

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2011.0489 ◽

2012 ◽

Vol 18 (8) ◽

pp. 603-613 ◽

Cited By ~ 1

Author(s):

Lingling Xia ◽

Qing Liu ◽

Wenjie Zhang ◽

Guangdong Zhou ◽

Yilin Cao ◽

...

Keyword(s):

Hair Follicle ◽

Oxygen Tension ◽

Human Hair ◽

Low Oxygen ◽

Human Hair Follicle ◽

Outer Root Sheath ◽

Root Sheath ◽

Low Oxygen Tension ◽

Outer Root Sheath Cells

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VEGF165 modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro

Journal of Dermatological Science ◽

10.1016/j.jdermsci.2013.10.002 ◽

2014 ◽

Vol 73 (2) ◽

pp. 152-160 ◽

Cited By ~ 18

Author(s):

Xian-Jie Wu ◽

Jian-Wei Zhu ◽

Jing Jing ◽

Dan Xue ◽

Hai Liu ◽

...

Keyword(s):

Hair Follicle ◽

Outer Root Sheath ◽

Root Sheath ◽

Outer Root Sheath Cells ◽

Sheath Cells

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The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers

Biomolecules ◽

10.3390/biom11020154 ◽

2021 ◽

Vol 11 (2) ◽

pp. 154

Author(s):

Hanluo Li ◽

Federica Francesca Masieri ◽

Marie Schneider ◽

Alexander Bartella ◽

Sebastian Gaus ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Hair Follicle ◽

Cell Populations ◽

Stem Cell Markers ◽

Outer Root Sheath ◽

Root Sheath ◽

Gene And Protein Expression ◽

Stem Cell Populations

Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.

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