Effect of siRNA Interference with Upregulated Gene 11 Expression on the Biological Functions of Osteosarcoma Cell Line MG63 and Their Mechanisms

This study investigated the effect of siRNA interference with URG11 expression on biological function of osteosarcoma cell line MG63 and its mechanism. MG63 cells cultured in vitro were apportioned to make up a control group (not transfected), NC-siRNA group (transfected nonspecific NC-siRNA), and URG11-siRNA group (transfected URG11-siRNA). The expression of URG11 was detected through reverse-transcription polymerase chain reaction (RT-PCR). The metastasis, infiltration, and apoptosis of MG63 cells were examined through CCK-8, Transwell, and flow cytometry techniques, respectively. The expressions of URG11 protein in each group of cells and of proteins β-catenin, c-Myc, cyclin D1, MMP-2, and survivin, which are related to the Wnt/β-catenin signal pathway, were visualized by the Western blot method. In comparison with the control group, transfected URG11-siRNA may reduce the expression levels of URG11 and URG11 mRNA, β-catenin, CyclinD1, c-Myc, MMP-2, and survivin proteins in MG63 cells, and may decrease the metastasis and infiltration of MG63 cells and enhance apoptosis; nevertheless, there was no obvious change in MG63 cells after NC-siRNA transfection. The proliferation and infiltration of MG63 cells can be inhibited by interference with URG11 expression, and the apoptosis of MG63 cells can be promoted by interference with URG11 expression. The mechanism of interference with URG11 expression may relate to inhibiting the activation of the Wnt/β-catenin pathway.

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Glucose Transporter Protein 1–Targeted RNA Interference Inhibits Growth and Invasion of the Osteosarcoma Cell Line MG63 In Vitro

Cancer Biotherapy & Radiopharmaceuticals ◽

10.1089/cbr.2010.0784 ◽

2010 ◽

Vol 25 (5) ◽

pp. 521-527 ◽

Cited By ~ 10

Author(s):

Jian Fan ◽

Jia-Qian Zhou ◽

Guang-Rong Yu ◽

Dong-Dong Lu

Keyword(s):

Rna Interference ◽

Cell Line ◽

Glucose Transporter ◽

Osteosarcoma Cell Line ◽

Osteosarcoma Cell ◽

Transporter Protein ◽

Osteosarcoma Cell Line Mg63

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Investigating the in vitro interactive effects of ionizing radiation and pamidronate in a canine osteosarcoma cell line

Veterinary and Comparative Oncology ◽

10.1111/j.1476-5810.2004.0045c.x ◽

2004 ◽

Vol 2 (2) ◽

pp. 99-100 ◽

Cited By ~ 2

Author(s):

S. C. Charney ◽

T. M. Fan ◽

L. P. de Lorimier ◽

H. I. Lacoste

Keyword(s):

Ionizing Radiation ◽

Cell Line ◽

Interactive Effects ◽

Osteosarcoma Cell Line ◽

Osteosarcoma Cell ◽

Canine Osteosarcoma

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LRP8 activates STAT3 to induce PD-L1 expression in osteosarcoma

Tumori Journal ◽

10.1177/0300891620952872 ◽

2020 ◽

pp. 030089162095287

Author(s):

Shiqin Zheng ◽

Yuxi Wei ◽

Yu Jiang ◽

Yi Hao

Keyword(s):

Cell Line ◽

Culture System ◽

Nuclear Translocation ◽

Interleukin 2 ◽

Dominant Negative ◽

Jurkat Cells ◽

Osteosarcoma Cell Line ◽

Osteosarcoma Cell ◽

Normal Tissues

Purpose: Targeting programmed death-ligand 1 (PD-L1) may be an effective intervention for osteosarcoma and PD-L1 expression is controlled by diverse regulatory factors. Low-density lipoprotein receptor-related protein 8 (LRP8) regulates osteoblast differentiation and it is unclear whether and how LRP8 could contribute to osteosarcoma pathogenesis. In this study, we investigated the LRP8/signal transducer and activator of transcription 3 (STAT3)/PD-L1 network in osteosarcoma. Methods: The expression of LRP8, STAT3, and PD-L1 was measured in osteosarcoma tissues and paired normal tissues. The effects of LRP8 on STAT3 and PD-L1 expression were investigated in an osteosarcoma cell line. The effects on immunosuppression were investigated in an in vitro co-culture system with Jurkat cell line and osteosarcoma cell line. The effects of LRP8 were blocked by a LRP8 neutralizing antibody, dominant-negative STAT3, or STAT3 inhibitor. Results: LRP8 was overexpressed in osteosarcoma compared to normal tissues and its level was correlated with phospho-STAT3 (p-STAT3) level in osteosarcoma tissues. In osteosarcoma cell lines, LRP8 increased p-STAT3 level and promoted nuclear translocation of STAT3. STAT3 activation also increased PD-L1 mRNA, protein, and promoter activity. In addition, LRP8 enhanced PD-L1 expression via STAT3. In a co-culture system, LRP8 overexpression in an osteosarcoma cell line impaired viability and interleukin-2 secretion of Jurkat cells and induced apoptosis of Jurkat cells. The effects of LRP8 could be blocked by neutralizing LRP8 antibody or STAT3 inhibitor. Blocking LRP8 inhibits proliferation and induces apoptosis of osteosarcoma cells. Conclusions: Our results provide evidence for a novel regulation network of LRP8/STAT3/PD-L1 in osteosarcoma and LRP8 may be a potential therapeutic target in osteosarcoma.

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Inhibitory Effects of Indomethacin in Human MNNG/HOS Osteosarcoma Cell Line In Vitro

Cancer Investigation ◽

10.1080/07357907.2019.1698592 ◽

2019 ◽

Vol 38 (1) ◽

pp. 23-36 ◽

Cited By ~ 2

Author(s):

Saied Mirshahidi ◽

Rosalia de Necochea-Campion ◽

Annie Moretta ◽

Nadine L. Williams ◽

Mark E. Reeves ◽

...

Keyword(s):

Cell Line ◽

Osteosarcoma Cell Line ◽

Osteosarcoma Cell ◽

Inhibitory Effects

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Formononetin Induces Apoptosis of Human Osteosarcoma Cell Line U2OS by Regulating the Expression of Bcl-2, Bax and MiR-375 In Vitro and In Vivo

Cellular Physiology and Biochemistry ◽

10.1159/000430220 ◽

2015 ◽

Vol 37 (3) ◽

pp. 933-939 ◽

Cited By ~ 19

Author(s):

Wei Hu ◽

ZengMing Xiao

Keyword(s):

Cell Line ◽

Cell Apoptosis ◽

Osteosarcoma Cell Line ◽

Osteosarcoma Cell ◽

Human Osteosarcoma Cell Line ◽

Human Osteosarcoma ◽

Human Osteosarcoma Cell ◽

U2os Cells

Background/Aims: Phytoestrogens are known to prevent tumor progression by inhibiting proliferation and inducing apoptosis in cancer cells. Formononetin is one of the main components of red clover plants, and is considered as a typical phytoestrogen. This study investigates formononetin induction of apoptosis of human osteosarcoma cell line U2OS by regulating Bcl-2 and Bax expression in vitro and in vivo. Methods: U2OS cells were treated with different concentrations of formononetin and the proliferation of the cells was measured using an MTT assay. Cell apoptosis was examined by flow cytometry. The levels of miR-375, Bax and Bcl-2 protein expression in treated cells were determined by Western blot and RT-PCR. The antitumor activity of formononetin was also evaluated in vivo in nude mice bearing orthotopic tumor implants. Results: High concentrations of formononetin significantly suppress the proliferation of U2OS cells and induce cell apoptosis. Moreover, compared to control group the expression of Bcl-2 and miR-375 decreases with formononetin in the U2OS cells, while Bax increases. Conclusion: Formononetin has inhibitory effects on the proliferation of U2SO cells, both in vitro and in vivo. This antitumor effect is directly correlated with formononetin concentration.

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