Novel High-Selectivity Fluorescent Probe for Detecting Alkaline Phosphatase Activity in Marine Environment

2020 ◽  
Vol 20 (6) ◽  
pp. 3348-3355 ◽  
Author(s):  
Jingya Sun ◽  
Mei Liu ◽  
Peng Wang ◽  
Zhiwei Gao ◽  
Jun Mu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Fluorescent Probe ◽  
Marine Environment ◽  
Photophysical Properties ◽  
Earth Metal ◽  
E Coli ◽  
Ph Values ◽  
Alp Activity ◽  
Alkaline Earth Metal Ions

In marine environment, alkaline phosphatase (ALP) is a nonspecific phosphatase with metal ions as its active site. Metal ions have different effects on ALP activity. Therefore, a probe that specifically detects ALP needs to be developed. In this paper, to eliminate the interference of acid phosphatase, we designed and synthesized a highly selective fluorescent probe CyP based on pH to detect ALP activity. The response mechanism of detecting ALP was explained. The photophysical properties, enzyme kinetics, stability, selectivity, and potential quantitative ability of the probe under different pH values were investigated. The effects of metal ions on the ALP activity of marine Chlorella vulgaris and Escherichia coli were also analyzed. Excessive metal ions such as Zn2+, Cu2+, Hg2+, Pb2+, and Cd2+ inhibit while Mn2+, Co2+, and alkaline-earth metal ions promote the ALP activity of Chlorella and E. coli.

Difference of Coordination between Alkali- and Alkaline-Earth-Metal Ions to a Symmetrical α,α′,δ,δ′-Tetramethylcucurbit[6]uril

2011 ◽  
Vol 50 (15) ◽  
pp. 6956-6964 ◽  
Author(s):  
Wen-Jian Chen ◽  
Da-Hai Yu ◽  
Xin Xiao ◽  
Yun-Qian Zhang ◽  
Qian-Jiang Zhu ◽  
...  
Keyword(s):  
Metal Ions ◽  
Alkaline Earth ◽  
Earth Metal ◽  
Alkaline Earth Metal ◽  
Alkaline Earth Metal Ions

Membranes for Separation of Alkali/Alkaline Earth Metal Ions: A Review

2021 ◽  
pp. 119640
Author(s):  
Yiran Wang ◽  
Wen Zhang ◽  
Xianjie Zeng ◽  
Tao Deng ◽  
Jianyou Wang
Keyword(s):  
Metal Ions ◽  
Alkaline Earth ◽  
Earth Metal ◽  
Alkaline Earth Metal ◽  
Alkaline Earth Metal Ions

scholarly journals Dansyl - Substituted Aza Crown Ethers: Complexation with Alkali, Alkaline Earth Metal Ions and Ammonium

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Nelly Mateeva ◽  
Shihab Deiab ◽  
Edikan Archibong ◽  
Donka Tasheva ◽  
Bereket Mochona ◽  
...  
Keyword(s):  
Metal Ions ◽  
Crown Ethers ◽  
Alkaline Earth ◽  
Earth Metal ◽  
Alkaline Earth Metal ◽  
Alkaline Earth Metal Ions ◽  
Aza Crown Ethers

Development of Ca2+ homeostasis in epithelial cells from embryonic and neonatal intestine

1992 ◽  
Vol 263 (3) ◽  
pp. G371-G379
Author(s):  
B. L. Black ◽  
J. O. Rogers
Keyword(s):  
Alkaline Phosphatase ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Fluorescent Probe ◽  
Cell Suspensions ◽  
Fura 2 ◽  
Alp Activity ◽  
High Viability ◽  
Cytoplasmic Compartment ◽  
Highly Correlated

The fluorescent probe fura-2 was used to assay Ca2+ levels in epithelial cell suspensions from embryonic and neonatal chick duodenum. Cell preparations maintained high viability, completely hydrolyzed fura-2/AM to fura-2, retained 92% of cellular fura-2 within the cytoplasmic compartment, and gave low autofluorescence values during assay. Fura-2 leakage from loaded cells occurred at all ages, but could be corrected for in subsequent calculations of cellular Ca2+. Cytoplasmic Ca2+ concentration was 76-80 nM in cells from 14- to 16-day embryonic intestine, rose significantly to 92-98 nM at 17-20 days, and reached 209 nM at 1-day post-hatch when assayed in buffers containing 1.3 mM Ca2+. The developmental rise in cytoplasmic Ca2+ was accompanied by an enhanced ability of cells to maintain a constant Ca2+ concentration at increased levels of extracellular Ca2+ and by a highly correlated rise in alkaline phosphatase (ALP) activity. Epithelial Ca2+ subsequently decreased to the "adult" value of 133-142 nM and was constant along the crypt-villus axis of neonatal intestine. These results verify that fura-2 can be used to compare baseline cytoplasmic Ca2+ values of epithelial cells from developing intestine, reveal that significant changes in Ca2+ homeostasis occur during ontogeny, and suggest that epithelial Ca2+ may modulate ALP activity during the differentiation of embryonic enterocytes.

scholarly journals Changes in Stereoselectivity in the Triose Aldol Condensation with Increased Concentration of Alkaline-Earth Metal Ions.

1988 ◽  
Vol 42b ◽  
pp. 546-549 ◽  
Author(s):  
Svein Morgenlie ◽  
Anja Oasmaa ◽  
Curt R. Enzell ◽  
Jan-Eric Berg ◽  
W. Michael Moore ◽  
...  
Keyword(s):  
Metal Ions ◽  
Alkaline Earth ◽  
Aldol Condensation ◽  
Earth Metal ◽  
Alkaline Earth Metal ◽  
Alkaline Earth Metal Ions
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