Bovine Serum Albumin Conjugation in Superparamagnetic/Poly(methyl methacrylate) Nanoparticles as an Alternative for Magnetic Enzyme-Linked Immunosorbent Assays

2021 ◽  
Vol 21 (11) ◽  
pp. 5493-5498
Author(s):  
Paulo Emilio Feuser ◽  
Camila Guindani ◽  
Jonathann Correa Possato ◽  
Jaqueline Pereira Guessi ◽  
Arthur Poester Cordeiro ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Protein Quantification ◽  
Elisa Assay ◽  
Poly Methyl Methacrylate ◽  
Transmission Electron ◽  
Superparamagnetic Properties ◽  
Significant Attention

Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM) and Lowry protein quantification assay. The superparamagnetic properties were confirmed by vibrating sample magnetometer. BSA conjugated MNPs-PMMA nanoparticles presented higher interactions with antibody than free BSA. The BSA + MNPs-PMMA nanoparticles (magnetic ELISA assay) reduced the time and increased the sensibility of traditional ELISA assay, reinforcing the idea that the use these nanomaterials are an excellent alternative for the immunoassays field.

Bovine serum albumin conjugation on poly(methyl methacrylate) nanoparticles for targeted drug delivery applications

2020 ◽  
Vol 56 ◽  
pp. 101490 ◽  
Author(s):  
Camila Guindani ◽  
Paulo Emílio Feuser ◽  
Arthur Poester Cordeiro ◽  
Alessandra Cristina de Meneses ◽  
Jonathann Corrêa Possato ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Targeted Drug Delivery ◽  
Bovine Serum ◽  
Poly Methyl Methacrylate ◽  
Targeted Drug ◽  
Drug Delivery Applications

Adsorption of bovine serum albumin onto poly(methyl methacrylate) stereocomplex films with a molecularly regulated nanostructure

2003 ◽  
Vol 41 (12) ◽  
pp. 1807-1812 ◽  
Author(s):  
Ken-Ichi Hamada ◽  
Keiko Yamashita ◽  
Takeshi Serizawa ◽  
Tatsuki Kitayama ◽  
Mitsuru Akashi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Poly Methyl Methacrylate

Fouling Characterization of Polysulfone-Grafted-Methyl Methacrylate Membrane

2013 ◽  
Vol 465-466 ◽  
pp. 819-823 ◽  
Author(s):  
Muhamad Zaini Yunos ◽  
Zawati Harun ◽  
Hatijah Basri ◽  
Hariati Taib ◽  
Ahmad Fauzi Ismail
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Pure Water ◽  
Water Flux ◽  
Optimum Time ◽  
Membrane Performance ◽  
Pure Water Flux

This study investigates the effects of different grafting time on the polysulfone grafted methyl methacrylate (PSf-g-MMA) membrane performance. PSf-g-MMA was successfully prepared via UV radiation method. Methyl methacrylate (MMA) and benzophenon (BP) were used as a monomer and initiator respectively. Membrane performances were measured based on permeation and rejection of bovine serum albumin (BSA). Degree of grafting was found to increase as grafting time increases and the optimum time was found at 100 minutes. The results revealed that the pure water flux and rejection were significantly improved with the addition of grafting time. The present of MMA also were found to improve fouling properties of the membrane.

Binding of Methyl Methacrylate to Bovine Serum Albumin

1980 ◽  
Vol 59 (12) ◽  
pp. 2056-2061 ◽  
Author(s):  
Sechiro Fujisawa ◽  
Echi Masuhara
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum
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