Adsorption of bovine serum albumin onto poly(methyl methacrylate) stereocomplex films with a molecularly regulated nanostructure

2003 ◽  
Vol 41 (12) ◽  
pp. 1807-1812 ◽  
Author(s):  
Ken-Ichi Hamada ◽  
Keiko Yamashita ◽  
Takeshi Serizawa ◽  
Tatsuki Kitayama ◽  
Mitsuru Akashi
2021 ◽  
Vol 21 (11) ◽  
pp. 5493-5498
Author(s):  
Paulo Emilio Feuser ◽  
Camila Guindani ◽  
Jonathann Correa Possato ◽  
Jaqueline Pereira Guessi ◽  
Arthur Poester Cordeiro ◽  
...  

Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM) and Lowry protein quantification assay. The superparamagnetic properties were confirmed by vibrating sample magnetometer. BSA conjugated MNPs-PMMA nanoparticles presented higher interactions with antibody than free BSA. The BSA + MNPs-PMMA nanoparticles (magnetic ELISA assay) reduced the time and increased the sensibility of traditional ELISA assay, reinforcing the idea that the use these nanomaterials are an excellent alternative for the immunoassays field.


2020 ◽  
Vol 56 ◽  
pp. 101490 ◽  
Author(s):  
Camila Guindani ◽  
Paulo Emílio Feuser ◽  
Arthur Poester Cordeiro ◽  
Alessandra Cristina de Meneses ◽  
Jonathann Corrêa Possato ◽  
...  

2013 ◽  
Vol 465-466 ◽  
pp. 819-823 ◽  
Author(s):  
Muhamad Zaini Yunos ◽  
Zawati Harun ◽  
Hatijah Basri ◽  
Hariati Taib ◽  
Ahmad Fauzi Ismail

This study investigates the effects of different grafting time on the polysulfone grafted methyl methacrylate (PSf-g-MMA) membrane performance. PSf-g-MMA was successfully prepared via UV radiation method. Methyl methacrylate (MMA) and benzophenon (BP) were used as a monomer and initiator respectively. Membrane performances were measured based on permeation and rejection of bovine serum albumin (BSA). Degree of grafting was found to increase as grafting time increases and the optimum time was found at 100 minutes. The results revealed that the pure water flux and rejection were significantly improved with the addition of grafting time. The present of MMA also were found to improve fouling properties of the membrane.


1980 ◽  
Vol 59 (12) ◽  
pp. 2056-2061 ◽  
Author(s):  
Sechiro Fujisawa ◽  
Echi Masuhara

Langmuir ◽  
2008 ◽  
Vol 24 (19) ◽  
pp. 10717-10722 ◽  
Author(s):  
Chuanxin He ◽  
Jianhong Liu ◽  
Xiaodong Ye ◽  
Laiyong Xie ◽  
Qianling Zhang ◽  
...  

Author(s):  
G. D. Gagne ◽  
M. F. Miller

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.


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