scholarly journals Measuring In Vivo Free Radical Production by the Outer Retina

2015 ◽  
Vol 56 (13) ◽  
pp. 7931 ◽  
Author(s):  
Bruce A. Berkowitz ◽  
Bryce X. Bredell ◽  
Christopher Davis ◽  
Marijana Samardzija ◽  
Christian Grimm ◽  
...  
Keyword(s):  
Free Radical ◽  
Outer Retina ◽  
Free Radical Production ◽  
Radical Production

scholarly journals Correcting QUEST Magnetic Resonance Imaging–Sensitive Free Radical Production in the Outer Retina In Vivo Does Not Correct Reduced Visual Performance in 24-Month-Old C57BL/6J Mice

2021 ◽  
Vol 62 (6) ◽  
pp. 24
Author(s):  
Bruce A. Berkowitz ◽  
Robert H. Podolsky ◽  
Karen Lins Childers ◽  
Robin Roberts ◽  
Michael Schneider ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Free Radical ◽  
Magnetic Resonance ◽  
Visual Performance ◽  
Outer Retina ◽  
Resonance Imaging ◽  
Free Radical Production ◽  
Radical Production

scholarly journals Direct evidence of iNOS-mediated in vivo free radical production and protein oxidation in acetone-induced ketosis

2008 ◽  
Vol 295 (2) ◽  
pp. E456-E462 ◽  
Author(s):  
Krisztian Stadler ◽  
Marcelo G. Bonini ◽  
Shannon Dallas ◽  
Danielle Duma ◽  
Ronald P. Mason ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Nadph Oxidase ◽  
Knockout Mice ◽  
Protein Oxidation ◽  
Ketone Bodies ◽  
Free Radical Production ◽  
Radical Production ◽  
Inos Knockout Mice

Diabetic patients frequently encounter ketosis that is characterized by the breakdown of lipids with the consequent accumulation of ketone bodies. Several studies have demonstrated that reactive species are likely to induce tissue damage in diabetes, but the role of the ketone bodies in the process has not been fully investigated. In this study, electron paramagnetic resonance (EPR) spectroscopy combined with novel spin-trapping and immunological techniques has been used to investigate in vivo free radical formation in a murine model of acetone-induced ketosis. A six-line EPR spectrum consistent with the α-(4-pyridyl-1-oxide)- N-t-butylnitrone radical adduct of a carbon-centered lipid-derived radical was detected in the liver extracts. To investigate the possible enzymatic source of these radicals, inducible nitric oxide synthase (iNOS) and NADPH oxidase knockout mice were used. Free radical production was unchanged in the NADPH oxidase knockout but much decreased in the iNOS knockout mice, suggesting a role for iNOS in free radical production. Longer-term exposure to acetone revealed iNOS overexpression in the liver together with protein radical formation, which was detected by confocal microscopy and a novel immunospin-trapping method. Immunohistochemical analysis revealed enhanced lipid peroxidation and protein oxidation as a consequence of persistent free radical generation after 21 days of acetone treatment in control and NADPH oxidase knockout but not in iNOS knockout mice. Taken together, our data demonstrate that acetone administration, a model of ketosis, can lead to protein oxidation and lipid peroxidation through a free radical-dependent mechanism driven mainly by iNOS overexpression.

The Effect of Antioxidants on free Radical Production During IN VIVO Metabolism of Carbon Tetrachloride

1983 ◽  
Vol 2 (3) ◽  
pp. 195-206 ◽  
Author(s):  
Paul B. McCay ◽  
M. Margaret King ◽  
Edward K. Lai ◽  
J. Lee Poyer
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
In Vivo Metabolism ◽  
Free Radical Production ◽  
Radical Production

scholarly journals Chemiluminescence response induced by mesenteric ischaemia/reperfusion: effect of antioxidative compounds ex vivo

2010 ◽  
Vol 3 (3) ◽  
pp. 105-108 ◽  
Author(s):  
Viera Nosáľová ◽  
Ružena Sotníková ◽  
Katarína Drábiková ◽  
Silvia Fialová ◽  
Daniela Košťálová ◽  
...  
Keyword(s):  
Free Radical ◽  
Cell Injury ◽  
Ex Vivo ◽  
Inhibitory Effect ◽  
High Morbidity ◽  
Mesenteric Ischaemia ◽  
Free Radical Production ◽  
Ischaemia Reperfusion ◽  
Radical Production

Chemiluminescence response induced by mesenteric ischaemia/reperfusion: effect of antioxidative compoundsex vivoIschaemia and reperfusion (I/R) play an important role in human pathophysiology as they occur in many clinical conditions and are associated with high morbidity and mortality. Interruption of blood supply rapidly damages metabolically active tissues. Restoration of blood flow after a period of ischaemia may further worsen cell injury due to an increased formation of free radicals. The aim of our work was to assess macroscopically the extent of intestinal pathological changes caused by mesenteric I/R, and to study free radical production by luminol enhanced chemiluminescence (CL) of ileal samples. In further experiments, the antioxidative activity of the drugs tested was evaluated spectrophotometrically by the use of the DPPH radical. We studied the potential protectiveex vivoeffect of the plant origin compound arbutin as well as of the pyridoindole stobadine and its derivative SMe1EC2. I/R induced pronounced haemorrhagic intestinal injury accompanied by increase of myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAGA) activity. Compared to sham operated (control) rats, there was only a slight increase of CL response after I/R, probably in association with neutrophil increase, indicated by enhanced MPO activity. All compounds significantly reduced the peak values of CL responses of the ileal samplesex vivo, thus reducing the I/R induced increase of free radical production. The antioxidants studied showed a similar inhibitory effect on the CL response influenced by mesenteric I/R. If provedin vivo, these compounds would represent potentially useful therapeutic antioxidants.

scholarly journals P-cresylsulphate, the main in vivo metabolite of p-cresol, activates leucocyte free radical production

2006 ◽  
Vol 22 (2) ◽  
pp. 592-596 ◽  
Author(s):  
E. Schepers ◽  
N. Meert ◽  
G. Glorieux ◽  
J. Goeman ◽  
J. Van der Eycken ◽  
...  
Keyword(s):  
Free Radical ◽  
Free Radical Production ◽  
Radical Production

Is There Evidence for Excessive Free Radical Production In Vivo during Ultrasound-Assisted Liposuction?

2003 ◽  
Vol 111 (1) ◽  
pp. 425-429 ◽  
Author(s):  
Jürgen Herr ◽  
Holger Hofheinz ◽  
Catherine Hertl ◽  
Rolf-Rüdiger Olbrisch ◽  
Eberhard Wieland
Keyword(s):  
Free Radical ◽  
Free Radical Production ◽  
Radical Production ◽  
Ultrasound Assisted

scholarly journals Using MRI to measure in vivo free radical production and perfusion dynamics in a mouse model of elevated oxidative stress and neurogenic atrophy

Redox Biology ◽  
2019 ◽  
Vol 26 ◽  
pp. 101308
Author(s):  
Bumsoo Ahn ◽  
Nataliya Smith ◽  
Debra Saunders ◽  
Rojina Ranjit ◽  
Parker Kneis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Mouse Model ◽  
Free Radical Production ◽  
Radical Production ◽  
Neurogenic Atrophy
Sign in / Sign up

Export Citation Format

Share Document