scholarly journals CD40 Expressed in Endothelial Cells Promotes Upregulation of ICAM-1 But Not Pro-Inflammatory Cytokines, NOS2 and P2X7 in the Diabetic Retina

2021 ◽  
Vol 62 (12) ◽  
pp. 22
Author(s):  
Jin-Sang Yu ◽  
Jad Daw ◽  
Jose-Andres C. Portillo ◽  
Carlos S. Subauste
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Cytokines ◽  
Diabetic Retina ◽  
Pro Inflammatory Cytokines

Inhibition of Pro-Inflammatory Cytokine Secretion by Select Antioxidants in Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 90 (1-2) ◽  
pp. 103-112 ◽  
Author(s):  
Michael J. Haas ◽  
Marilu Jurado-Flores ◽  
Ramadan Hammoud ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Coronary Artery ◽  
Inflammatory Cytokines ◽  
Inflammatory Cytokine ◽  
Culture Media ◽  
Cytokine Secretion ◽  
Human Coronary Artery ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract. Inflammatory and oxidative stress in endothelial cells are implicated in the pathogenesis of premature atherosclerosis in diabetes. To determine whether high-dextrose concentrations induce the expression of pro-inflammatory cytokines, human coronary artery endothelial cells (HCAEC) were exposed to either 5.5 or 27.5 mM dextrose for 24-hours and interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF α) levels were measured by enzyme immunoassays. To determine the effect of antioxidants on inflammatory cytokine secretion, cells were also treated with α-tocopherol, ascorbic acid, and the glutathione peroxidase mimetic ebselen. Only the concentration of IL-1β in culture media from cells exposed to 27.5 mM dextrose increased relative to cells maintained in 5.5 mM dextrose. Treatment with α-tocopherol (10, 100, and 1,000 μM) and ascorbic acid (15, 150, and 1,500 μM) at the same time that the dextrose was added reduced IL-1β, IL-6, and IL-8 levels in culture media from cells maintained at 5.5 mM dextrose but had no effect on IL-1β, IL-6, and IL-8 levels in cells exposed to 27.5 mM dextrose. However, ebselen treatment reduced IL-1β, IL-6, and IL-8 levels in cells maintained in either 5.5 or 27.5 mM dextrose. IL-2 and TNF α concentrations in culture media were below the limit of detection under all experimental conditions studied suggesting that these cells may not synthesize detectable quantities of these cytokines. These results suggest that dextrose at certain concentrations may increase IL-1β levels and that antioxidants have differential effects on suppressing the secretion of pro-inflammatory cytokines in HCAEC.

CD40 expression and function in human dermal endothelial cells is regulated by pro-inflammatory cytokines

1998 ◽  
Vol 16 ◽  
pp. S176
Author(s):  
Sareeta R. Singh ◽  
Diane Hollenbaugh ◽  
Robert A. Swerlick
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Cytokines ◽  
Cd40 Expression ◽  
And Function ◽  
Pro Inflammatory Cytokines

scholarly journals Multiple expression assessments of ACE2 and TMPRSS2 SARS-CoV-2 entry molecules in the urinary tract and their associations with clinical manifestations of COVID-19

2020 ◽  
Author(s):  
Xiaohan Ren ◽  
Xiyi Wei ◽  
Guangyao Li ◽  
Shancheng Ren ◽  
Xinglin Chen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Urinary Tract ◽  
Inflammatory Cytokines ◽  
Kidney Diseases ◽  
Single Cells ◽  
Expression Patterns ◽  
Clinical Manifestations ◽  
Gene Set Enrichment Analysis ◽  
Human Testis ◽  
Pro Inflammatory Cytokines

AbstractBackgroundSince December 2019, the novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first spread quickly in Wuhan, China, then globally. From previously published evidence, ACE2 and TMPRSS2, are both pivotal entry molecules that enable cellular infection by SARS-CoV-2. Meanwhile, increased expression of pro-inflammatory cytokines, or a “cytokine storm,” is associated with multiple organ dysfunction syndrome that is often observed in critically ill patients.MethodsWe investigated the expression pattern of ACE2 and TMPRSS2 in major organs in the human body, especially under specific disease conditions. Multiple sequence alignment of ACE2 in different species was used to explain animal susceptibility. Moreover, the cell-specific expression patterns of ACE2 and cytokine receptors in the urinary tract were assessed using single-cell RNA sequencing (scRNA-seq). Additional biological relevance was determined through Gene Set Enrichment Analysis (GSEA) using an ACE2 specific signature.ResultsOur results revealed that ACE2 and TMPRSS2 were highly expressed in genitourinary organs. ACE2 was highly and significantly expressed in the kidney among individuals with chronic kidney diseases or diabetic nephropathy. In single cells, ACE2 was primarily enriched in gametocytes in the testis, and renal proximal tubules. The receptors for pro-inflammatory cytokines, especially IL6ST, were remarkably concentrated in endothelial cells, macrophages, and spermatogonial stem cells in the testis, and renal endothelial cells, which suggested the occurrence of alternative damaging mechanisms via autoimmune attacks.ConclusionsThis study provided new insights into the pathogenicity mechanisms of SARS-CoV-2 that underlie the clinical manifestations observed in the human testis and kidney. These observations might substantially facilitate the development of effective treatments for this rapidly spreading disease.

scholarly journals Internalization ofStaphylococcus aureusby Bovine Endothelial Cells is Associated with the Activity State of NF-κB and Modulated by the Pro-inflammatory Cytokines TNF-α and IL-1β

2008 ◽  
Vol 67 (2) ◽  
pp. 169-176 ◽  
Author(s):  
J. Oviedo-Boyso ◽  
J. G. Barriga-Rivera ◽  
J. J. Valdez-Alarcón ◽  
A. Bravo-Patiño ◽  
A. Cárabez-Trejo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Cytokines ◽  
Tnf Α ◽  
Bovine Endothelial Cells ◽  
Activity State ◽  
Pro Inflammatory Cytokines

Manidipine reduces pro-inflammatory cytokines secretion in human endothelial cells and macrophages

2010 ◽  
Vol 62 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Sara Costa ◽  
Francesca Zimetti ◽  
Matteo Pedrelli ◽  
Giovanni Cremonesi ◽  
Franco Bernini
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Cytokines ◽  
Human Endothelial Cells ◽  
Cytokines Secretion ◽  
Pro Inflammatory Cytokines

An Extract of the Medicinal Mushroom Agaricus blazei Murill Differentially Stimulates Production of Pro-inflammatory Cytokines in Human Monocytes and Human Vein Endothelial Cells in vitro

Inflammation ◽  
2006 ◽  
Vol 29 (4-6) ◽  
pp. 147-153 ◽  
Author(s):  
Soosaipillai Bernardshaw ◽  
Geir Hetland ◽  
Linda Kathrine Ellertsen ◽  
Anne Merete Aaland Tryggestad ◽  
Egil Johnson
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Cytokines ◽  
Human Monocytes ◽  
Medicinal Mushroom ◽  
Agaricus Blazei ◽  
Agaricus Blazei Murill ◽  
Pro Inflammatory Cytokines

scholarly journals Protection of cultured brain endothelial cells from cytokine-induced damage by α-melanocyte stimulating hormone

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4774 ◽  
Author(s):  
András Harazin ◽  
Alexandra Bocsik ◽  
Lilla Barna ◽  
András Kincses ◽  
Judit Váradi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Rat Brain ◽  
Inflammatory Cytokines ◽  
Nuclear Translocation ◽  
Brain Endothelial Cells ◽  
Melanocyte Stimulating Hormone ◽  
Brain Pericytes ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines ◽  
Stimulating Hormone

The blood–brain barrier (BBB), an interface between the systemic circulation and the nervous system, can be a target of cytokines in inflammatory conditions. Pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) induce damage in brain endothelial cells and BBB dysfunction which contribute to neuronal injury. The neuroprotective effects of α-melanocyte stimulating hormone (α-MSH) were investigated in experimental models, but there are no data related to the BBB. Based on our recent study, in which α-MSH reduced barrier dysfunction in human intestinal epithelial cells induced by TNF-α and IL-1β, we hypothesized a protective effect of α-MSH on brain endothelial cells. We examined the effect of these two pro-inflammatory cytokines, and the neuropeptide α-MSH on a culture model of the BBB, primary rat brain endothelial cells co-cultured with rat brain pericytes and glial cells. We demonstrated the expression of melanocortin-1 receptor in isolated rat brain microvessels and cultured brain endothelial cells by RT-PCR and immunohistochemistry. TNF-α and IL-1β induced cell damage, measured by impedance and MTT assay, which was attenuated by α-MSH (1 and 10 pM). The peptide inhibited the cytokine-induced increase in brain endothelial permeability, and restored the morphological changes in cellular junctions visualized by immunostaining for claudin-5 and β-catenin. Elevated production of reactive oxygen species and the nuclear translocation of NF-κB were also reduced by α-MSH in brain endothelial cells stimulated by cytokines. We demonstrated for the first time the direct beneficial effect of α-MSH on cultured brain endothelial cells, indicating that this neurohormone may be protective at the BBB.

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