Acute Inhibitory Effects of Antidepressants on Lacrimal Gland Secretion in the Anesthetized Rat

Dry eye syndrome (DES) is a corneal disease often characterized by an irritating, itching feeling in the eyes and light sensitivity. Inflammation and endoplasmic reticulum (ER) stress may play a crucial role in the pathogenesis of DES, although the underlying mechanism remains elusive. Aster koraiensis has been used traditionally as an edible herb in Korea. It has been reported to have wound-healing and inhibitory effects against insulin resistance and inflammation. Here, we examined the inhibitory effects of inflammation and ER stress by A. koraiensis extract (AKE) in animal model and human retinal pigmented epithelial (ARPE-19) cells. Oral administration of AKE mitigated DE symptoms, including reduced corneal epithelial thickness, increased the gap between lacrimal gland tissues in experimental animals and decreased tear production. It also inhibited inflammatory responses in the corneal epithelium and lacrimal gland. Consequently, the activation of NF-κB was attenuated by the suppression of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Moreover, AKE treatment ameliorated TNF-α-inducible ocular inflammation and thapsigargin (Tg)-inducible ER stress in animal model and human retinal pigmented epithelial (ARPE-19) cells. These results prove that AKE prevents detrimental functional and histological remodeling on the ocular surface and in the lacrimal gland through inhibition of inflammation and ER stress, suggesting its potential as functional food material for improvement of DES.

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Effect of calcium antagonism or chelation on rabbit lacrimal gland secretion and membrane potentials

The Journal of Physiology ◽

10.1113/jphysiol.1980.sp013331 ◽

1980 ◽

Vol 304 (1) ◽

pp. 397-403 ◽

Cited By ~ 15

Author(s):

Stella Y. Botelho ◽

Darlene A. Dartt

Keyword(s):

Lacrimal Gland ◽

Membrane Potentials ◽

Gland Secretion ◽

Calcium Antagonism

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Interaction of EGF family growth factors and neurotransmitters in regulating lacrimal gland secretion

Experimental Eye Research ◽

10.1016/s0014-4835(03)00202-1 ◽

2004 ◽

Vol 78 (3) ◽

pp. 337-345 ◽

Cited By ~ 40

Author(s):

Darlene A. Dartt

Keyword(s):

Growth Factors ◽

Lacrimal Gland ◽

Gland Secretion ◽

Family Growth ◽

Egf Family

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c-Jun NH2-terminal kinase mediates interleukin-1beta-induced inhibition of lacrimal gland secretion

Journal of Neurochemistry ◽

10.1111/j.1471-4159.2005.03529.x ◽

2006 ◽

Vol 96 (1) ◽

pp. 126-135 ◽

Cited By ~ 14

Author(s):

Driss Zoukhri ◽

Elizabeth Macari ◽

Sun H. Choi ◽

Claire L. Kublin

Keyword(s):

Lacrimal Gland ◽

Gland Secretion ◽

Interleukin 1Beta

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Alteration in nerves and neurotransmitter stimulation of lacrimal gland secretion in the TSP-1−/− mouse model of aqueous deficiency dry eye

Mucosal Immunology ◽

10.1038/s41385-018-0002-y ◽

2018 ◽

Vol 11 (4) ◽

pp. 1138-1148 ◽

Cited By ~ 8

Author(s):

Sumit Bhattacharya ◽

Laura García-Posadas ◽

Robin R. Hodges ◽

Helen P. Makarenkova ◽

Sharmila Masli ◽

...

Keyword(s):

Mouse Model ◽

Dry Eye ◽

Lacrimal Gland ◽

Gland Secretion ◽

Stimulation Of

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Stimulation of rabbit lacrimal gland secretion with biologically active peptides

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1988.254.3.g300 ◽

1988 ◽

Vol 254 (3) ◽

pp. G300-G306

Author(s):

D. A. Dartt ◽

M. Shulman ◽

K. L. Gray ◽

S. R. Rossi ◽

C. Matkin ◽

...

Keyword(s):

Protein Secretion ◽

Lacrimal Gland ◽

Excretory Duct ◽

Biologically Active ◽

Gland Secretion ◽

Dependent Manner ◽

Active Peptides ◽

Biologically Active Peptides ◽

Dose Dependent ◽

Stimulation Of

To determine whether biologically active peptides can stimulate lacrimal gland secretion, we measured fluid and protein secretion from the cannulated lacrimal gland excretory duct of anesthetized rabbits after arterial injection of various peptides. Vasoactive intestinal peptide (VIP, 0.003-3 nmol) and porcine histidine isoleucine-containing peptide (PHI, 0.01-3 nmol) stimulated fluid and protein secretion in a dose-dependent manner. Cholecystokinin octapeptide (CCK-8, 0.01-3 nmol) stimulated fluid but not protein secretion. Neither bombesin nor eledoisin in doses as high as 3 nmol stimulated fluid or protein secretion. When combinations of high or low doses of VIP and the cholinergic agonist acetylcholine (ACh) were injected simultaneously, fluid and protein secretion was additive. We concluded that VIP and PHI stimulated secretion of lacrimal gland fluid and protein, CCK-8 stimulated secretion of fluid, and bombesin and eledoisin did not stimulate either fluid or protein secretion. VIP and ACh, both found in lacrimal gland nerve endings, stimulate lacrimal gland fluid and protein secretion by separate pathways.

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