Systems view of Bordetella pertussis booster vaccination in adults primed with whole-cell vs. acellular vaccine

Background: Bordetella pertussis, a highly contagious respiratory. Notably, the resurgence of pertussis has recently been associated with the lacking production of vaccine virulence factors. This study aimed to screen pertactin (Prn) and filamentous hemagglutinin (Fha) production in Iran with 50 years' whole cell vaccine (WCV) immunization program. Methods: Overall, 130 B. pertussis isolates collected from Pertussis Reference Laboratory of Iran during 2005-2018. Real-time PCR was performed by targeting IS481, ptxP, IS1001 and IS1002 for species confirmation of B. pertussis. Western-blot was used to evaluate the expression of virulence factors (pertactin and filamentous hemagglutinin). Results: All tested B. pertussis isolates expressed Prn and all except two isolates expressed Fha. We have sequenced genomes of these strains and identified differences compared with genome reference B. pertussis Tohama I. Conclusion: Many countries reporting Prn and Fha-deficiency due to acellular vaccine (ACV) pressure. Our results demonstrate in a country with WCV history, Fha-deficient isolates may rise independently. However, Prn-deficient isolates are more under the ACV pressure in B. pertussis isolates. Continues surveillance will provide a better understanding of the effect of WCV on the evolution of the pathogen deficiency.

Download Full-text

Long-term cellular memory immunity against Bordetella pertussis and other components of the DTP-IPV-Hib vaccine in Dutch children: comparison of a whole cell vaccine (WCV) with an acellular vaccine (ACV)

http://isrctn.org/> ◽

10.1186/isrctn65428640 ◽

2012 ◽

Cited By ~ 1

Author(s):

Lotte Hendrikx

Keyword(s):

Bordetella Pertussis ◽

Cell Vaccine ◽

Cellular Memory ◽

Whole Cell ◽

Whole Cell Vaccine ◽

Dutch Children ◽

Acellular Vaccine

Download Full-text

Quantity and Quality of Antibodies After Acellular Versus Whole-cell Pertussis Vaccines in Infants Born to Mothers Who Received Tetanus, Diphtheria, and Acellular Pertussis Vaccine During Pregnancy: A Randomized Trial

Clinical Infectious Diseases ◽

10.1093/cid/ciz778 ◽

2019 ◽

Vol 71 (1) ◽

pp. 72-80 ◽

Cited By ~ 7

Author(s):

Nasamon Wanlapakorn ◽

Kirsten Maertens ◽

Sompong Vongpunsawad ◽

Jiratchaya Puenpa ◽

Thao Mai Phuong Tran ◽

...

Keyword(s):

Growth Inhibition ◽

Bordetella Pertussis ◽

Controlled Trial ◽

Booster Vaccination ◽

Control Group ◽

Term Infants ◽

Growth Inhibition Assay ◽

Whole Cell ◽

Vaccine Responses

Abstract Background The blunting effect of pertussis immunization during pregnancy on infant antibody responses induced by whole-cell pertussis (wP) vaccination is not well-defined. Methods This randomized controlled trial (NCT02408926) followed term infants born to mothers vaccinated with tetanus, diphtheria, and acellular pertussis (Tdap) vaccine during pregnancy in Thailand. Infants received either acellular pertussis (aP)- or wP-containing vaccine at 2, 4, 6, and 18 months of age. A comparison group comprised wP-vaccinated children born to mothers not vaccinated during pregnancy. Antibodies against pertussis toxin (PT), filamentous hemagglutinin (FHA), and pertactin (PRN) were evaluated using commercial enzyme-linked immunosorbent assays. Functionality of antibodies against Bordetella pertussis was measured using Bordetella pertussis growth inhibition assay. Results After maternal Tdap vaccination, 158 infants vaccinated with aP-containing vaccines possessed higher antibody levels (P < .001) against all tested B. pertussis antigens postpriming compared to 157 infants receiving wP-containing vaccines. At 1 month postbooster, only anti-FHA and anti-PRN antibodies were still significantly higher (P < .001) in the aP group. Significantly higher anti-PT and anti-FHA (P < .001), but not anti-PRN immunoglobulin G, were observed among 69 wP-vaccinated infants born to control mothers compared with wP-vaccinated infants of Tdap-vaccinated mothers after primary and booster vaccination. The antibody functionality was higher in all wP-vaccinated infants at all times. Conclusions Maternal Tdap vaccination inhibited more pertussis-specific responses in wP-vaccinated infants compared to aP-vaccinated infants, and the control group of unvaccinated women had highest PT-specific responses, persisting until after the booster dose. Antibody functionality was better in the wP groups. Clinical Trials Registration NCT02408926. Infant whole-cell pertussis (wP) vaccine responses are blunted after maternal Tdap vaccination. Pertussis antibody titers are higher in acellular pertussis (aP)– than wP-vaccinated infants of immunized mothers, yet quality of antibodies, measured as serum-mediated bacterial growth inhibition, is better after wP than aP vaccination.

Download Full-text

Reduced Risk of Pertussis in Whole-Cell Compared to Acellular Vaccine Recipients Is Not Supported When Data Are Stratified by Age

Clinical Infectious Diseases ◽

10.1093/cid/cit552 ◽

2013 ◽

Vol 57 (11) ◽

pp. 1658-1660 ◽

Cited By ~ 3

Author(s):

P. Andre ◽

D. R. Johnson ◽

D. P. Greenberg ◽

M. D. Decker

Keyword(s):

Whole Cell ◽

Reduced Risk ◽

Acellular Vaccine

Download Full-text

Bordetella pertussis outer membrane vesicle vaccine confers equal efficacy in mice with milder inflammatory responses compared to a whole-cell vaccine

Scientific Reports ◽

10.1038/srep38240 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 23

Author(s):

René H. M. Raeven ◽

Jolanda Brummelman ◽

Jeroen L. A. Pennings ◽

Larissa van der Maas ◽

Wichard Tilstra ◽

...

Keyword(s):

Outer Membrane ◽

Bordetella Pertussis ◽

Inflammatory Responses ◽

Membrane Vesicle ◽

Cell Vaccine ◽

Outer Membrane Vesicle ◽

Whole Cell ◽

Whole Cell Vaccine ◽

Equal Efficacy

Download Full-text

Restricted antibody response to Bordetella pertussis filamentous hemagglutinin induced by whole-cell and acellular pertussis vaccines

Infectious Diseases ◽

10.3109/23744235.2015.1093655 ◽

2015 ◽

Vol 48 (2) ◽

pp. 127-132 ◽

Cited By ~ 1

Author(s):

Hossein Asgarian-Omran ◽

Maryam Golara ◽

Sara Abdolmaleki ◽

Shadi Sadat Navabi ◽

Hadi Alipour ◽

...

Keyword(s):

Antibody Response ◽

Bordetella Pertussis ◽

Whole Cell ◽

Filamentous Hemagglutinin

Download Full-text

Economic evaluation of pertussis prevention by whole-cell and acellular vaccine in Germany

European Journal of Pediatrics ◽

10.1007/s004310050837 ◽

1998 ◽

Vol 157 (5) ◽

pp. 395-401 ◽

Cited By ~ 26

Author(s):

G. Tormans ◽

E. Van Doorslaer ◽

P. van Damme ◽

R. Clara ◽

H. J. Schmitt

Keyword(s):

Economic Evaluation ◽

Whole Cell ◽

Acellular Vaccine

Download Full-text

Primary Immunization of Infants With an Acellular Pertussis Vaccine in a Double-Blind Randomized Clinical Trial

PEDIATRICS ◽

10.1542/peds.82.3.293 ◽

1988 ◽

Vol 82 (3) ◽

pp. 293-299

Author(s):

Margareta Blennow ◽

Marta Granström ◽

Eva Jäätmaa ◽

Patrick Olin

Keyword(s):

Clinical Trial ◽

Randomized Clinical Trial ◽

Pertussis Vaccine ◽

Acellular Pertussis Vaccine ◽

Cell Vaccine ◽

Primary Immunization ◽

Double Blind ◽

Whole Cell ◽

Whole Cell Vaccine ◽

Acellular Vaccine

The rate of adverse reactions and the immunogenicity of a two-component acellular pertussis vaccine as compared with a plain whole-cell vaccine and a placebo were evaluated for primary immunization in 319 6-month-old infants in a double-blind randomized clinical trial. The acellular vaccine produced few and mild systemic and local reactions. Fever (≥38°C) occurred in 6% to 8% of acellular vaccinees as opposed to 25% to 37% of whole-cell vaccinees. Redness (≥1 cm) appeared in 2% to 13% of the acellular vaccine and 24% to 32% of the whole-cell vaccine recipients. Antibody response to pertussis toxin measured in a neutralization test was obtained in 97% to 100% of the infants receiving either two or three doses of the acellular vaccine as compared to 59% after three doses of whole-cell vaccine.

Download Full-text