scholarly journals Pre-beta-very low density lipoproteins as precursors of beta-very low density lipoproteins. A model for the pathogenesis of familial dysbetalipoproteinemia (type III hyperlipoproteinemia).

1988 ◽  
Vol 82 (2) ◽  
pp. 628-639 ◽  
Author(s):  
D A Chappell
Keyword(s):  
Low Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Familial Dysbetalipoproteinemia

Two-dimensional immunoelectrophrophoretic pattern of low- and very-low-density lipoproteins, with particular reference to Fredrickson's type III.

1977 ◽  
Vol 23 (2) ◽  
pp. 186-194 ◽  
Author(s):  
J E Rerabek
Keyword(s):  
Laboratory Diagnosis ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Two Dimensional ◽  
Lipoprotein Profile ◽  
Very Low Density Lipoproteins ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Crossed Immunoelectrophoresis ◽  
Antigenic Properties

Abstract I report the pattern of low-density lipoproteins (LDL) and very-low-density lipoproteins (VLDL) as revealed by two-dimensional (crossed) immunoelectrophoresis in hyperlipoproteinemia types, II, III, and IV, preliminarily diagnosed by means of a complex lipoprotein profile examination. The higher affinity of Oil Red stain for VLDL- than for LDL-immunoprecipitation zones is peculiar to this lipoprotein class, and crossed immunoelectrophoretograms may thus be helpful for laboratory diagnosis of types IIb and IV in problematic cases. The position and the medium affinity for Oil Red of "broad beta" zones appearing in immunoelectrophoretograms of type III hyperlipoproteinemia are suggested as useful criteria for the diagnosis of this condition. From the structural and staining characteristics of VLDL-immunoprecipitation zones, alteration of both composition and antigenic properties of VLDL in hyperlipoproteinemias IIb-IV is deduced. I present in detail the procedure for crossed immunoelectrophoresis as well as that for the lipoprotein profile examination used in this study.

Improved Techniques for Assessment of Serum Lipoprotein Patterns. II. Rapid Method for Diagnosis of Type III Hyperlipoproteinemia without Ultracentrifugation

1973 ◽  
Vol 19 (10) ◽  
pp. 1139-1141 ◽  
Author(s):  
Heinrich Wieland ◽  
Dietrich Seidel
Keyword(s):  
Rapid Method ◽  
Serum Lipoprotein ◽  
Low Density Lipoproteins ◽  
Plasma Lipoproteins ◽  
Agarose Gel ◽  
Low Density ◽  
Electrophoretic Separation ◽  
Very Low Density Lipoproteins ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia

Abstract Based on the previously described technique [Clin. Chem.19, 737 (1973)] of precipitating plasma lipoproteins with polyanions after their electrophoretic separation in gels, a new method is presented for diagnosing type III hyperlipoproteinemia without need for ultracentrifugation or immunologic techniques. Used in the procedure are 0.1 mol/liter MgCl2, 1.5 g/liter heparin, and 10 g/liter NaCl to visualize selectively the very-low-density lipoproteins in agarose gel after electrophoresis. The technique is simple, inexpensive, accessible to every laboratory, and provides the answer in less than 2 h after electrophoresis of the patient’s whole serum. The results obtained are the same as those obtained by ultracentrifugation followed by lipoprotein electrophoresis of the isolated fractions.

Diagnosis of Type III Hyperlipoproteinemia by Chromatography of Plasma Lipoproteins on Columns Containing Agarose

1975 ◽  
Vol 21 (13) ◽  
pp. 1887-1891 ◽  
Author(s):  
James Shepherd ◽  
Christopher J Packard ◽  
Frances J Dryburgh ◽  
Jane L H C Third
Keyword(s):  
Low Density Lipoproteins ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Type Iii ◽  
Elution Pattern ◽  
Type Iii Hyperlipoproteinemia ◽  
Primary Type ◽  
Relative Deficiency

Abstract Agarose column chromatography has been used to separate plasma lipoproteins into very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL) and high-density lipoproteins (HDL). Applied to the diagnosis of primary type III hyperlipoproteinemia, the procedure is capable of demonstrating three characteristic and specific changes from normality in the elution pattern of lipoproteins from patients with this condition. In the type III profile there is (a) incomplete separation of VLDL from putative LDL material, (b) early elution of the type III LDL with respect to a normal LDL marker, and (c) relative deficiency of type III LDL with elution characteristics of normal LDL. We advocate the use of this method in the diagnosis of type III hyperlipoproteinemia.

scholarly journals Degradation of lipoproteins by human monocyte-derived macrophages. Evidence for two distinct processes for the degradation of abnormal very-low-density lipoprotein from subjects with type III hyperlipidaemia

1984 ◽  
Vol 218 (1) ◽  
pp. 101-111 ◽  
Author(s):  
A K Soutar ◽  
B L Knight
Keyword(s):  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Human Monocyte ◽  
Normal Subjects ◽  
Peritoneal Macrophages ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Free Medium ◽  
Very Low Density Lipoproteins ◽  
Type Iii

Human blood monocyte-derived macrophages that had been cultured in medium containing human serum for 7 days degraded the abnormal very-low-density lipoproteins (VLDL) from the plasma of subjects with type III hyperlipoproteinaemia by two distinct saturable processes. One process was stimulated when cells from normal subjects were preincubated with lipoprotein-free medium, was inhibited by excess unlabelled low-density lipoproteins (LDL) and was absent from cells from subjects with homozygous familial hypercholesterolaemia; on these criteria it was identified as an LDL-receptor-dependent process. Degradation by the second process was of equal magnitude in both cell types and was unaffected by excess unlabelled LDL or acetylated LDL. The activity of this process was reduced when the cells were preincubated in lipoprotein-free medium. The abnormal VLDL from the plasma of cholesterol-fed rabbits were also degraded by this process, which was similar to that in mouse peritoneal macrophages mediated by the receptor for VLDL of beta-electrophoretic mobility [Goldstein, Ho, Brown, Innerarity & Mahley (1980) J. Biol. Chem. 255, 1839-1848].

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