Lipoprotein Profile in Populations from Regions of the Russian Federation: ESSE-RF Study

This study aimed to describe the dyslipidemia prevalence and pattern among adult populations from different regions (n = 13) of the Russian Federation (RF). Randomly selected samples (n = 22,258, aged 25–64) were studied according to the ESSE-RF protocol. Lipoprotein parameters were estimated by routine methods. Statistical analyses were performed using R software (v.3.5.1). The overall dyslipidemia prevalence was 76.1% (76.9/75.3% for men/women). In women, total cholesterol (TC) and low-density lipoprotein (LDL)-C levels gradually increased with age (from 4.72 to 5.93 and from 2.76 to 3.79 mmol/L, respectively); in men, they reached a maximum by 45–54 (5.55 and 3.55 mmol/L, respectively) and then decreased. No differences in high-density lipoprotein (HDL)-C in men of different ages were found, but slight decreases in HDL-C and apo AI were observed in women by 55–64 years. No pronounced associations between education and lipid levels in men were observed; higher-educated women showed significantly better lipoprotein profiles. Similar associations between lipids and income level were detected. Women from rural areas had higher TC and triglycerides than urban residents. Regardless of sex, rural residents had higher HDL-C and apo AI, and reduced apo B/apo AI. Conclusion: Information on the peculiarities of dyslipidemia prevalence and lipoprotein profile depending on sex, age, residential place, and socioeconomic status is useful for assessing the global ASCVD risk, and for risk modeling based on national data.

1 Prospective associations of aerobic fitness and lipoprotein subclasses in a cohort of norwegian schoolchildren: the active smarter kids (ASK) study

AimAerobic fitness is associated with cardiometabolic risk factors in children. Associations with traditional measures of lipid metabolism are uncertain. We investigated whether higher levels of fitness benefit lipid metabolism by exploring cross-sectional and prospective associations between aerobic fitness and a comprehensive lipoprotein profile.MethodsWe used targeted proton nuclear magnetic resonance (1H NMR) spectroscopy to profile 29 measures of lipoprotein metabolism for 811 fifth-grade Norwegian schoolchildren (50.1% girls; mean age 10.2 years). Serum samples were taken on two occasions across the academic year. Aerobic fitness was measured at baseline using the Andersen aerobic fitness test. We used multiple linear regression adjusted for potential confounders to examine both cross-sectional and prospective — adjusted for baseline lipoprotein measure — associations between aerobic fitness and lipoprotein profiles.ResultsHigher levels of aerobic fitness were associated with all measures of lipoprotein metabolism in the cross-sectional analysis. There were inverse associations with the apolipoprotein B-containing (apo B) lipoprotein subclasses, including cholesterol and triglyceride concentration. The associations between aerobic fitness and the concentration of high-density lipoprotein (HDL) particles were divergent between larger and smaller subclasses. In the prospective analysis, the inverse associations between aerobic fitness and the measures of larger apo B-containing lipoprotein subclasses persisted as did all but one of the associations with triglyceride concentrations. Additional adjustment for adiposity attenuated most associations in both cross-sectional and prospective models, but an independent effect of fitness remained for certain measures.ConclusionsHigher levels of aerobic fitness are associated with a favourable lipoprotein profile, partly independent of adiposity. Associations tended to be stronger and more consistent over time for the larger apo B-containing lipoprotein measures and those of triglyceride concentration. Our results suggest that improving children’s fitness levels should have beneficial effects on lipoprotein metabolism, though a concomitant reduction in adiposity would likely be more effective.ReferencesAnderssen SA, Cooper AR, Riddoch C, Sardinha LB, Harro M, Brage S, et al. Low cardiorespiratory fitness is a strong predictor for clustering of cardiovascular disease risk factors in children independent of country, age and sex. Eur J Cardiovasc Prev Rehabil 2007.Mintjens S, Menting MD, Daams JG, van Poppel MNM, Roseboom TJ, Gemke RJBJ. Cardiorespiratory fitness in childhood and adolescence affects future cardiovascular risk factors: a systematic review of longitudinal studies. Sports Med 2018 Nov 1;48(11):2577–605.

Effects of Cranberry Juice Supplementation on Cardiovascular Disease Risk Factors in Adults with Elevated Blood Pressure: A Randomized Controlled Trial

Emerging cardiovascular disease (CVD) risk factors, including central vascular function and HDL efflux, may be modifiable with food-based interventions such as cranberry juice. A randomized, placebo-controlled, crossover trial was conducted in middle-aged adults with overweight/obesity (n = 40; mean BMI: 28.7 ± 0.8 kg/m2; mean age: 47 ± 2 years) and elevated brachial blood pressure (mean systolic/diastolic BP: 124 ± 2/81 ± 1 mm Hg). Study participants consumed 500 mL/d of cranberry juice (~16 fl oz; 27% cranberry juice) or a matched placebo juice in a randomized order (8-week supplementation periods; 8-week compliance break), with blood samples and vascular measurements obtained at study entry and following each supplementation period. There was no significant treatment effect of cranberry juice supplementation on the primary endpoint of central systolic blood pressure or central or brachial diastolic pressure. Cranberry juice significantly reduced 24-h diastolic ambulatory BP by ~2 mm Hg compared to the placebo (p = 0.05) during daytime hours. Cranberry juice supplementation did not alter LDL-C but significantly changed the composition of the lipoprotein profile compared to the placebo, increasing the concentration of large LDL-C particles (+29.5 vs. −6.7 nmol/L; p = 0.02) and LDL size (+0.073 vs. −0.068 nm; p = 0.001). There was no effect of treatment on ex vivo HDL efflux in the total population, but exploratory subgroup analyses identified an interaction between BMI and global HDL efflux (p = 0.02), with greater effect of cranberry juice in participants who were overweight. Exploratory analyses indicate that baseline C-reactive protein (CRP) values may moderate treatment effects. In this population of adults with elevated blood pressure, cranberry juice supplementation had no significant effect on central systolic blood pressure but did have modest effects on 24-hr diastolic ambulatory BP and the lipoprotein profile. Future studies are needed to verify these findings and the results of our exploratory analyses related to baseline health moderators.

Outstanding Improvement of the Advanced Lipoprotein Profile in Subjects with New-Onset Type 1 Diabetes Mellitus After Achieving Optimal Glycemic Control

Background: An altered lipoprotein profile is often hidden in normolipidemic subjects with uncontrolled type 1 diabetes mellitus (T1D). Conceivably, its amelioration after glycemic control may be overlooked in controlled T1D subjects. In this regard, we investigated the influence of glycemic optimization on lipoprotein subfraction parameters in normolipidemic new-onset T1D subjects.Methods: Twelve subjects at onset of T1D (5 women and 7 men) were studied. Serum lipid and advanced lipoprotein profiles were determined by routine laboratory methods and 1H-NMR spectroscopy shortly after diabetes diagnosis (baseline), and after achieving optimal glycemic control (HbA1c<7%) at a follow-up of 11.3 to 25.6 weeks.Results: Advanced lipoprotein analysis revealed a significant reduction from baseline in plasma concentrations of triglycerides (TG) and cholesterol (C), and in apolipoprotein (Apo)B-containing lipoproteins of treated subjects (VLDL-TG: -21%, LDL-TG: -36%, IDL-TG: -32%, LDL-TG: -36%, P<0.05; VLDL-C: -46%, IDL-C: -46%, LDL-C: -16%; P<0.05). Additionally, neither VLDL-TG and LDL-TG nor LDL-C of T1D subjects at follow-up differed from those of non-diabetic subjects. Decreased VLDL and LDL lipids were mainly attributed to concomitant reductions in the concentration of medium-sized VLDL (-39%) and medium-sized LDL (34%) and, to a lesser extent, to large-sized LDL (13%), respectively. Notably, proatherogenic IDL characteristics and related surrogates of atherogenicity were resolved upon intensive glycemic control. However, neither the concentration of HDL-P, nor the relative proportion of HDL sizes was influenced by the glucose-lowering therapy.Conclusions: In otherwise normolipidemic T1D subjects according to the conventional lipid profile, the achievement of optimal glycemic control after diabetes onset is associated with normalization of profound derangements in ApoB-containing lipoproteins (VLDL, IDL, and LDL). Whether higher cardiovascular risk in poorly controlled T1D is partially mediated by these proatherogenic lipid alterations should be elucidated by further studies.